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Citations for Qiagen :
351 - 400 of 2762 citations for HLA A*0201 WT 1 complex Protein Human HEK293 His since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2021Quote: ... The His-tagged BASP1 derivatives were prepared using Ni-NTA beads following the manufacturers instructions (Qiagen). Purified proteins were dialysed into Buffer D and stored at −80°C.
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bioRxiv - Immunology 2022Quote: ... and His-tagged Fabs were isolated from cell supernatants using Ni-NTA columns (Qiagen, Hilden, Germany). IgGs ...
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bioRxiv - Biophysics 2023Quote: ... the glass was additionally treated with biotinylated anti-hexahistidine monoclonal antibody (Penta-His Biotin Conjugate; Qiagen) as in Duchi et al.23 and Dulin et al.31.
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bioRxiv - Microbiology 2024Quote: ... sodium dodecyl-sulfate polyacrylamide gel electrophoresis (SDS-PAGE) and Western blotting using Penta-His antibody (QIAGEN)) were pooled and concentrated using Amicon filter devices with 10 kDa molecular weight cut-off (Millipore ...
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bioRxiv - Immunology 2022Quote: ... The cleaved protein was passed over a 1 mL Ni-NTA agarose (Qiagen) gravity column to remove TEV protease ...
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bioRxiv - Molecular Biology 2023Quote: The eluted protein was mixed with 1 ml Strep-Tactin Superflow agarose (Qiagen) and incubated at 4 °C for 30 min ...
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bioRxiv - Cell Biology 2020Quote: RNA was isolated from mEos3.2-lamin A/C WT#21 cells using the RNeasy mini plus kit (Qiagen) according to manufacturer’s instructions ...
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bioRxiv - Cell Biology 2021Quote: ... RNA was extracted from mid–log phase WT and ykl075cΔ cells using the RNeasy kit (Qiagen, Germantown, MD). RNA quality was analyzed with Agilent 2100 Bioanalyzer using a Plant RNA Nano chip and RNA Integrity Number (RIN ...
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bioRxiv - Molecular Biology 2022Quote: ... genomic DNA from wild type (WT) and YBX1 KO cells was extracted using DNeasy Blood & Tissue Kit (Qiagen) and amplified using the primers 5’-GGGTAATGAGGCTACAACTGTTT-3’ and 5’-GTCAACTCTAACACATTTCCACGTAT-3’ ...
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bioRxiv - Cancer Biology 2020Quote: ... Two fragments encompassing 1.3 times the wt HBV genome were purified using the QIAquick PCR Purification Kit (Qiagen) after PCR using the following primer pairs ...
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bioRxiv - Physiology 2022Quote: ... tissue was isolated from kidneys from WT and Irs4-/- mice using miRNeasy Mini kits from Qiagen (Germantown, MD). First-strand cDNA was synthesized by iScript™ cDNA synthesis kit (Bio-Rad ...
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bioRxiv - Developmental Biology 2023Quote: RNA was extracted from four replicates of WT and NR2F2-KO COV434 cells using RNeasy Plus Kit (QIAGEN). The RNA quality and quantity were assayed on NanoDrop spectrophotometer and Qubit fluorometer (Thermo Fisher Scientific) ...
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bioRxiv - Cell Biology 2022Quote: Genomic DNA was isolated from U2OS WT and ZNF524 KO clones using QIAamp DNA Blood Mini Kit (Qiagen) with RNase treatment ...
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bioRxiv - Genetics 2024Quote: Total RNA was extracted from the dorsal skin of HL and WT male mice using RNeasy Kit (Qiagen). RNA samples corresponding to postnatal days ...
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bioRxiv - Cell Biology 2021Quote: ... human myosin-18A (synthesized by Qiagen)
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bioRxiv - Molecular Biology 2021Quote: RNA was isolated from INS-1 and human islets 48 hours post transfection using RNeasy cleanup kit (Qiagen) according to manufacturer’s protocol ...
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bioRxiv - Biophysics 2021Quote: ... Media containing his-tagged Ecads was passed through a chromatography column containing Ni-NTA agarose beads (Qiagen). Beads were then washed with a pH 7.5 biotinylation buffer (25mM HEPES ...
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bioRxiv - Biophysics 2022Quote: ... Media containing his-tagged Ecads was passed through a chromatography column containing Ni-NTA agarose beads (Qiagen). Beads were then washed with a pH 7.5 biotinylation buffer (25 mM HEPES ...
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bioRxiv - Molecular Biology 2022Quote: ... The JAK3 PCR fragment was purified and cloned into the pQE-Tri System His-Strep2 vector (Qiagen). The final pQE-JAK3 construct was used to transfect GC using the Xfect transfection kit (Takara Bio ...
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bioRxiv - Synthetic Biology 2020Quote: ... The plates were coated with 100 μL of 4 μg/mL murine anti-His mAb (Qiagen, #34660), and after blocking ...
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bioRxiv - Biochemistry 2021Quote: ... His-tagged Sic1PY conjugates (polyubiquitylated Sic1PY, Ubn-Sic1PY) were purified by incubating with Ni-NTA resin (Qiagen) at 4 °C for 1 h ...
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bioRxiv - Bioengineering 2023Quote: ... Purification of His-tagged GA-MatryoshCaMP6s was performed with Ni-NTA agarose beads (Qiagen, cat. No: 30210). Before applying the lysate ...
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bioRxiv - Biochemistry 2024Quote: ... The His-Sortase A 7M enzyme was purified away from the reaction using Ni-NTA resin (Qiagen) with 50 mM Tris pH 7.5 ...
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bioRxiv - Plant Biology 2024Quote: ... The His-fused ZmbZIP75 was expressed in Transetta (DE3) cells and purified with Ni-HA beads (QIAGEN). The recombinant protein was incubated with biotin-labeled probe ...
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bioRxiv - Biochemistry 2024Quote: ... Protein in the soluble lysate was bound to 1 mL Ni-NTA resin (Qiagen), washed with 5 mL low salt wash buffer (20 mM Tris HCl pH 8.0 ...
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bioRxiv - Immunology 2021Quote: ... RNA of cells exposed to SARS-CoV-2 WT was isolated with the QIAamp Viral RNA Mini Kit (Qiagen) according to the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2021Quote: RNA was extracted from cultured mid-log phase WT or ykl075cΔ cells using the RNeasy kit (QIAGEN, Germantown, MD). Genomic DNA contamination was removed using TURBO DNA-free Kit (Ambion ...
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bioRxiv - Molecular Biology 2022Quote: Total RNA was extracted from asynchronous or metaphase-arrested WT and Suv39h dn ESCs (500.000 cells) using RNeasy mini Kit (74106, Qiagen). Extracted RNA was treated twice with Turbo DNA free (AM1907 ...
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bioRxiv - Cell Biology 2021Quote: Total RNA was extracted from adult WT and homozygous CatSpert-134del mutant male testes using RNeasy Mini kit (QIAGEN). 500 ng of the extracted RNA was used for cDNA synthesis using iScriptTM cDNA Synthesis kit (Bio-Rad ...
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bioRxiv - Microbiology 2020Quote: Viral RNA was extracted from 10,000 TCID50 WT and NS1R38A virus containing inoculum using the QIAamp Viral RNA mini kit (Qiagen), according to the manufactures protocol ...
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bioRxiv - Plant Biology 2021Quote: RNA was extracted from three Col-0_rpw8 and three Col-0 WT individual plants using the RNeasy Plant Mini Kit (QIAgen) and treated with RNase-Free DNase Set (QIAGEN) ...
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bioRxiv - Genomics 2021Quote: Tissue lysates were prepared from 30 pooled 2 dpf WT and MZdnmt3aa-/- mutant by homogenization using TissueLyser LT (QIAGEN) with zirconia beads for 10 s at 50 Hz ...
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bioRxiv - Genetics 2020Quote: ... and cells treated with transfection mix without siRNA (Wild type (WT)) or non-targeting control siRNA (scrambled (SCR)) (Qiagen catalog SI03650318 ...
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bioRxiv - Cell Biology 2024Quote: Erc1 was genetically knocked down in B16-F1 WT cells using specific siRNA oligonucleotides targeting Rab6ip (Erc1) (Qiagen; 1027416). The cells were transfected using Lullaby transfection reagent according to the manufacturer’s instructions with a pool of 10 nM of Mus musculus Rab6ip siRNA (2.5 nM each ...
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bioRxiv - Molecular Biology 2023Quote: ... EGFP-ZMYM2(WT) (pAS4329) or EGFP-ZMYM2(SIMmut) (pAS4330) plasmids using Polyfect Transfection Reagent (Qiagen, Cat. No. ID: 301105) for 24 hours ...
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bioRxiv - Immunology 2023Quote: ... 24 hours) treated and untreated control U2OS WT and ZBTB48 KO clones using the RNeasy Plus Mini Kit (Qiagen) with additional on-column DNaseI digestion following the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2023Quote: ... genomic DNA from each OCT4 knockout clone or WT was extracted using DNeasy blood and tissue kit (QIAGEN, 69504) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2024Quote: ... RNA from four different biological replicates from WT and HET cells was extracted using the miRNeasy Mini Kit (Qiagen) followed by on-column DNAse digestion ...
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bioRxiv - Immunology 2024Quote: Total RNA was extracted from the FACS sorted WT FRCs (CD45- CD31-PDPN+) cells using RNeasy Mini kit (Qiagen) and cDNA was synthesized using cDNA reverse transcription kit (BioRad) ...
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bioRxiv - Cancer Biology 2024Quote: ... tumors and intestinal organoids of ApcMin/+-Hkdc1ΔIEC and WT littermate mice was extracted using the RNeasy Mini Kit (Qiagen) according to the manufacturer’s protocol ...
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bioRxiv - Bioengineering 2020Quote: ... each SeraCare panel member was diluted 1:10 in human plasma to a final volume of 100μL and processed by QIAGEN QIAamp viral mini kit ...
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bioRxiv - Biochemistry 2020Quote: ... DNA constructs were amplified in Escherichia coli DH5α and purified using a Hi-Speed Plasmid Maxi Kit (Qiagen).
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bioRxiv - Biochemistry 2020Quote: ... (2010) using commercial antibodies directed against the His-tag following the instructions of the manufacture (Qiagen, Hilden, Germany).
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bioRxiv - Microbiology 2020Quote: A 6.5 nM concentration of His-tagged 16055 SOSIP.v8.3 trimer in TBS was added to a 96-well Ni-NTA plate (Qiagen) for 2 h at RT ...
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bioRxiv - Microbiology 2020Quote: ... His-SFP1 was purified using Ni-NTA affinity purification under native conditions using the standard manufacturer’s protocol (Qiagen). Recombinant His-SFP1 was used to immunize female New Zealand white rabbits (Covalab ...
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bioRxiv - Biochemistry 2021Quote: DNA constructs were amplified in Escherichia coli DH5α and purified using a Hi-Speed Plasmid Maxi Kit (Qiagen).
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bioRxiv - Plant Biology 2024Quote: ... All fractions were analyzed by SDS-polyacrylamide gel electrophoresis (SDS-PAGE) and Western blotting using anti-His (Qiagen) and anti-strep (MilliporeSigma ...
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bioRxiv - Biochemistry 2022Quote: ... Lysates of His-tagged talin constructs were purified by Ni-NTA-sepharose affinity chromatography (Ni2+-nitrilotriacetic acid, Qiagen), followed by a gel filtration column (Superdex 200 ...
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bioRxiv - Biochemistry 2022Quote: ... Lysates of His-tagged vinculin constructs were purified by Ni-NTA-sepharose affinity chromatography (Ni2+-nitrilotriacetic acid, Qiagen), followed by a Q-Sepharose ion exchange column ...
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bioRxiv - Cell Biology 2023Quote: ... siRNA for ANXA2 (Human, Qiagen, Hs_ANXA2_8, SI02632385) or siRNA for Anxa2 (Mouse ...