Labshake search
Citations for Qiagen :
351 - 400 of 458 citations for Annexin V Solution APC since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Neuroscience 2021Quote: ... The titer of the individual lentiviral solution was quantified by Lenti-X p24 Rapid Titer Kit (Qiagen, 632200) at the first round of production and then Lenti-X GoStix Plus Kit (Qiagen ...
-
bioRxiv - Microbiology 2020Quote: ... pH 8.0) containing 15 mg/mL of lysozyme + 15 µL of proteinase K solution (20 mg/mL, Qiagen), and then incubated for 8–10 min ...
-
bioRxiv - Microbiology 2020Quote: ... with YFV-C (1-10) K8ac peptide were grown from a solution containing 0.1 M HEPES (pH 7.0) (QIAGEN), 30% PEG 6000 (QIAGEN ...
-
bioRxiv - Microbiology 2020Quote: ... with YFV-C (1-10) K4ac/K8ac peptide were grown from a solution containing 0.1 M HEPES (pH 7.0) (QIAGEN), 30% PEG 6000 (QIAGEN ...
-
bioRxiv - Microbiology 2022Quote: ... The dissected midguts were lysed in the lysis solution supplied with the kit using the TissueLyser system (Qiagen). The library for RNA-seq was prepared using a HyperPrep kit (KAPA ...
-
bioRxiv - Microbiology 2021Quote: ... RNA was extracted from 140 μL of PEDV-HB3 solution using a QIAamp Viral RNA mini kit (QIAGEN), following the manufacturer’s instructions ...
-
bioRxiv - Biochemistry 2022Quote: ... Drops were prepared by mixing 0.1 µl of protein solution with 0.1 µl of precipitant solution containing 0.2 M magnesium chloride and 20% PEG3350 (NeXtal PACT Suite, Qiagen). Crystals were harvested after 2 weeks ...
-
bioRxiv - Biochemistry 2022Quote: ... or 0.2 or 0.4 μL of the protein solution and 0.2 μL of a series of commercially available crystallization screens (Qiagen). Conditions that provided initial hits were optimized using bespoke screens prepared at the crystallization facility ...
-
bioRxiv - Microbiology 2024Quote: ... Bacteria were centrifuged at 4°C and washed with pre-cold PBS containing RNA stabilization solution (RNAprotect, Qiagen) before flash freezing of the pellets and storage at -80°C ...
-
bioRxiv - Evolutionary Biology 2024Quote: ... the lysates were cooled on ice and mixed with 200 µL of Protein Precipitation Solution (Qiagen ref. 158910) for 5 minutes ...
-
bioRxiv - Cell Biology 2020Quote: ... Cells were washed once with 1 % BSA and incubated with a solution containing 200 μg/ml RNase A (Qiagen) and 50 μg/ml Propodium Iodide (Sigma ...
-
bioRxiv - Immunology 2021Quote: ... A total of 30 μL RNA solution was collected from each sample using a RNeasy Mini Kit (QIAGEN, Germany) according to the manufacturer’s instructions ...
-
bioRxiv - Genomics 2020Quote: ... The total RNA solution from 12 wells are passed through one column of RNeasy mini kit (cat.74104, QIAGEN) to obtain approximately 100 g of purified total RNA ...
-
Vaccinia virus-based vaccines confer protective immunity against SARS-CoV-2 virus in Syrian hamstersbioRxiv - Immunology 2021Quote: ... A total of 30 μl RNA solution was collected from each sample using an RNeasy Mini Kit (QIAGEN, Germany) according to the manufacturer’s instructions ...
-
bioRxiv - Cancer Biology 2021Quote: ... De-paraffinization of FFPE tumor and adjacent normal specimens was performed using QIAGEN de-paraffinization solution (QIAGEN, Valencia, CA). DNA and RNA from 19 tumor and adjacent normal samples was extracted using the AllPrep DNA/RNA FFPE Kit (QIAGEN ...
-
bioRxiv - Microbiology 2021Quote: ... A total of 30 μL RNA solution was collected from each lung sample using RNeasy Mini Kit (QIAGEN, Germany) according to the manufacturer’s instructions ...
-
bioRxiv - Microbiology 2020Quote: ... Crystals of Brd4(BD1) with YFV-C (1-10) K4ac peptide were grown from a solution containing 0.2 M sodium chloride (QIAGEN), 0.1 M Tris (pH 7.0 ...
-
bioRxiv - Biophysics 2021Quote: The soluble fraction of the disrupted cell solution was isolated by centrifugation at 14,000xg for 20 minutes and loaded into a Ni-NTA column system (Qiagen). The column was washed with a volume corresponding to 5 column volumes (CV ...
-
bioRxiv - Genetics 2021Quote: ... 10 mM imidazole (final concentration) was added to the sample and the solution was bound to 0.5 ml NiNTA resin (Qiagen). The resin was washed with NTA buffer A1 (50 mM Tris-HCl pH 7.5 ...
-
bioRxiv - Genetics 2021Quote: ... We extracted DNA from approximately 1-2 million cells of actively growing culture by first pelleting the cells and resuspending them in 1.0 mL Cell Lysis Solution (Qiagen). The samples were incubated with RNase A solution at 37°C for 40 min ...
-
bioRxiv - Microbiology 2023Quote: ... Bacterial biomass from strains grown on SKV agar plates was resuspended directly in 300 μl of PowerBead Solution (Qiagen) and vortexed to mix ...
-
bioRxiv - Plant Biology 2022Quote: ... The lysed solution was centrifuged at 12,000 × g for 30 min and the supernatant was incubated with Ni-NTA agarose (Qiagen) at 4 □ for 3 hours ...
-
bioRxiv - Physiology 2023Quote: Livers were powdered in liquid nitrogen then homogenized using GCTU solution (6M guanidinium chloride and 1.5M thiourea) and a hand-held homogenizer (TissueRuptor II, Qiagen). Liver and plasma aliquots were injected into 96-well plates (2.4 mL/well ...
-
bioRxiv - Cancer Biology 2023Quote: ... red blood cells were lysed in five times the amount of red blood cell lysis solution (Qiagen, Hilden, Germany), followed by 15 min of incubation at room temperature (24°C) ...
-
bioRxiv - Synthetic Biology 2022Quote: ... single flies were homogenized in 30μL Tris-EDTA buffer pH 8.0 with 5M NaCl and Proteinase-K (proK) Qiagen solution (from Qiagen #69504). Samples were incubated at 37°C for 30 minutes and then at 95°C for four minutes ...
-
bioRxiv - Evolutionary Biology 2023Quote: ... Bacterial biomass from isolates grown on SKV agar plates was resuspended directly in 300 μl of PowerBead Solution (Qiagen) and vortexed to mix ...
-
bioRxiv - Microbiology 2023Quote: TG was quantified in snap-frozen liver tissue stored at −80 □C until cryo-grinding in liquid nitrogen and 50 ±5 mg tissue added 0.9 mL of a 2:1 chloroform:methanol solution and homogenized 1 min at 50 os/sec using a TissueLyser LT (Qiagen) with beads ...
-
bioRxiv - Biophysics 2024Quote: The supernatant solution was incubated with pre-equilibrated with lysis buffer containing 7mM imidazole Ni–NTA agarose beads (QIAGEN) and kept for binding for 3 hours on a rotating platform at 4°C ...
-
bioRxiv - Microbiology 2024Quote: ... approximately 3 g of each soil was mixed with 2 volumes of LifeGuard Soil Preservation Solution (Qiagen, Hilden, Germany) directly in the field ...
-
bioRxiv - Genomics 2020Quote: ... at which point we combined the resulting solution with 12 mL of PB buffer and followed standard MinElute PCR Purification Kit (Qiagen) protocol ...
-
bioRxiv - Genetics 2020Quote: ... Analyses of HTT CAG repeat size in both HttQ111 mice and in patient fibroblasts was performed by PCR using human-specific HTT primers and Taq PCR Core Kit with Q solution (Qiagen), as previously described (17 ...
-
bioRxiv - Evolutionary Biology 2021Quote: ... The solidified plugs were submerged in Lysis Buffer solution (Bionano) and Proteinase K (Puregene/Qiagen, 66.8 µl per ml Buffer) for two hours at 50°C ...
-
bioRxiv - Immunology 2021Quote: ... and distal colon (the final 3.5 cm adjacent to the rectum) and the single tumors were immediately preserved in 2 ml of RNAlater stabilization solution (Qiagen) according to manufacturer instructions and stored at −20°C ...
-
bioRxiv - Microbiology 2021Quote: Swab RNA was extracted from 0.2 ml of swab solutions (1ml of DMEM with 2% fetal bovine serum [Cytiva]) using QIAamp Viral RNA Minikit (QIAGEN) and subjected to real-time RT-PCR for viral RNA quantitation [40] using QuantiTect Probe RT-PCR Kit (Qiagen ...
-
bioRxiv - Microbiology 2021Quote: ... The beetle microbiomes were extracted by crushing individual whole beetles with a micropestle in 450 µL of MBL solution from the QIAamp BiOstic Bacteremia DNA kit by Qiagen© (one beetle per tube) ...
-
bioRxiv - Cancer Biology 2021Quote: ... the transposed DNA fragments were extracted from the reaction solution using the Mini Elute PCR purification kit (Qiagen Cat# 28004) and then amplified by a 12-cycle PCR amplification step with specific primers as described by Buenrostro et al. ...
-
The spatial landscape of progression and immunoediting in primary melanoma at single cell resolutionbioRxiv - Cancer Biology 2022Quote: ... The retrieved tissue mROIs were deposited with 2 µl PBS into PCR tubes containing 18 µl of lysis buffer: 1:16 mix of Proteinase K solution (QIAGEN) in PKD buffer (QIAGEN) ...
-
bioRxiv - Cell Biology 2022Quote: RNA was isolated from bone marrow cells using the gDNA eliminator solution for purification of total RNA (RNeasy Plus Mini Kit, Qiagen). cDNA was synthesized by using AMV reverse transcriptase with random primers (Promega) ...
-
bioRxiv - Bioengineering 2022Quote: ... A high salt solution in combination with isopropanol was utilized for precipitation of RNA before purification with the RNeasy Mini Kit (Qiagen) according to the manufacturer’s protocol ...
-
bioRxiv - Neuroscience 2021Quote: ... DRG neurons were transfected in serum free DMEM solution with predesigned fluorescently labelled siRNA directly against mouse TMEM120A (TACAN) from Qiagen-FlexiTube (cat ...
-
bioRxiv - Bioengineering 2021Quote: ... The histidine-tagged TEV and MBP were extracted from the solution using a Ni-NTA agarose resin (Qiagen, Hilden, Germany) as described in[20].
-
bioRxiv - Microbiology 2022Quote: ... from the top A horizon were immediately collected for DNA extractions in 15 mL tubes containing 5 mL Lifeguard Soil Preservation Solution (QIAGEN) using chilled soil-processing trays ...
-
bioRxiv - Genetics 2022Quote: ... The tissue powder was then transferred to a 1.5 mL centrifuge tube where 400 μL of 1% PVP-40 Buffer AP1 solution and 4 μL of RNase A were added (Qiagen DNeasy Plant Kit Qiagen Inc ...
-
bioRxiv - Genomics 2020Quote: 3 million cells from cell lines or patient samples were pelleted and resuspended in 1 mL of Cell Lysis Solution (Qiagen) mixed with 500 μg of RNase A ...
-
bioRxiv - Genomics 2020Quote: ... The ethanol was then poured out and the pellet was air-dried for 15 minutes before resuspension in 50 to 100 μL of DNA Hydration Solution (Qiagen). DNA was sequenced with paired end Illumina sequencing at 30x coverage.
-
bioRxiv - Cancer Biology 2021Quote: ... Tissue microregions were deposited with 2 µl PBS into PCR tubes containing 18 µl of lysis buffer: 1:16 mix of Proteinase K solution (QIAGEN) in PKD buffer (QIAGEN) ...
-
bioRxiv - Biochemistry 2021Quote: ... before extraction solution was added to achieve a concentration of 20 mg/mL before immediate lysis in a benchtop TissueLyser LT (Qiagen) operated at 50 Hz for 3 minutes ...
-
bioRxiv - Immunology 2021Quote: ... Cells were rinsed at rested at 37°C for a minimum of 1h before undergoing red-blood-cell lysis by 5-10 ml RBC lysis solution (Qiagen) for 20 min at room temperature ...
-
bioRxiv - Molecular Biology 2023Quote: ... and sperm using the Bio Basic EZ-10 Spin Column Genomic Minipreps kit for animal sample according to the manufacturer’s protocol except for sperm samples for which the kit ACL lysis solution was replaced by 300 μl of RTL lysis buffer (Qiagen) and 3 μl of mercaptoethanol ...
-
bioRxiv - Microbiology 2023Quote: ... Cells were sorted into 96-well plates containing lysis buffer with proteinase K solution in PKD buffer (1:16) (Qiagen) and were stored at -80ºC ...