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Citations for Qiagen :
3851 - 3900 of 3900 citations for 4 Methoxy 2 2 3 4 5 5 Hexacb 13C12 99% 50 Ug Ml In Toluene since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2024Quote: ... S2 cells were seeded at 1 × 106 cells/ml in a 6-well plate and transfected using Effectene (Qiagen, Germantown, MD). For coimmunoprecipitation (coIP ...
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bioRxiv - Developmental Biology 2024Quote: ... and 100 μg/mL streptomycin and were transfected with 800 ng of pAc5-3xFLAG-HRJDs using Effectene Transfection Reagent (QIAGEN, 301427) following the manufacturer’s protocol ...
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bioRxiv - Immunology 2024Quote: ... We collected 2.5 ml of CSF and whole blood for transcriptional profiling in blood PAX-gene® (Pre-AnalytiX, Qiagen, USA) tubes ...
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bioRxiv - Microbiology 2024Quote: ... using standards previously generated.65 We collected 2.5 ml of CSF and whole blood for transcriptional profiling in blood PAX-gene® (Pre-AnalytiX, Qiagen, USA) tubes ...
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bioRxiv - Evolutionary Biology 2020Quote: All genomic DNA was extracted using a Bioline Isolate II Genomic DNA Extraction Kit (Cat#. BIO-52067) following the recommended protocol with an additional DNAse free RNaseA (100mg/ml) (Qiagen cat. #19101) treatment before column purification ...
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bioRxiv - Bioengineering 2019Quote: ... Up to ∼2 million cells were resuspended in 100 μl High-TE buffer (10 mM Tris-Cl, pH 8, 10 mM EDTA, 25-100 μg/ml RNase A (QIAGEN, Cat. # 19101)) and lysed by adding 2.5 μl 20% SDS ...
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bioRxiv - Genetics 2019Quote: ... genomic DNA was extracted from a saturated culture of 100 ml under anaerobic condition (YPD) using the genomic tip-100 kit (Qiagen, Courtaboeuf, FRANCE). Paired-end Illumina sequencing libraries were prepared from sonicated genomic DNA according to manufacturer protocols (Genomic DNA Sample Preparation ...
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bioRxiv - Microbiology 2021Quote: ... ethanol-stored flies were rehydrated in water and transferred to 1.5 ml well plates for homogenisation using a bead beater (Qiagen Tissue Lyzer II). Protein was digested using Proteinase K ...
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bioRxiv - Microbiology 2020Quote: ... The cell lysate was further treated with 25 µL of proteinase K (20 mg/mL) (Qiagen Singapore Pte. Ltd, Cat. No. 19133), gently mixed by flicking of tube and incubated at 56 °C for 20 min ...
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bioRxiv - Biochemistry 2021Quote: ... His-tagged proteins were recovered from the clarified lysate using a drip column containing a 5.0 ml bed volume of Ni-NTA resin (Qiagen, Mississauga, ON, Canada). After extensive washing with buffer A ...
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bioRxiv - Biochemistry 2020Quote: ... A final 370,000 x g centrifugation at 4°C for 45 min yielded a myosin-containing supernatant that was loaded onto a 1 mL nickel-nitrilotriacetic acid agarose (Ni-NTA) (Qiagen, Cat# 30210) column equilibrated with column buffer (50 mM Tris-HCl pH 8.0 ...
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bioRxiv - Plant Biology 2020Quote: ... the root and leaf dry matter were ground into powder by placing the samples into glass vials with three opposing blades and shaking at a frequency of 30 Hz for 10 minutes using a Qiagen TissueLyser II (Qiagen, ML, USA). Ground root and leaf percent N was determined by the Dumas method using the Elementar rapid N exceed analyzer (Elementar Americas Inc ...
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Cell-free DNA as a biomarker for prostate cancer: elevated concentration and decreased fragment sizebioRxiv - Cancer Biology 2020Quote: ... DNA was extracted from 7 to 55 mL of plasma using the Qiagen QIAamp Circulating Nucleic Acid Kit (Qiagen, Redwood City, CA), and double eluted with 40 μL of Qiagen Elution Buffer ...
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bioRxiv - Microbiology 2022Quote: ... Samples and the DNA extraction negative control (1 ml PBS) were processed for DNA extraction using the QIAamp DNA Microbiome kit (Qiagen, Hilden, Germany) following the manufacturer’s protocol (Depletion of Host DNA protocol).
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bioRxiv - Microbiology 2022Quote: ... aeruginosa cells were lysed after 18 hours using 2.5mg/mL lysozyme and incubated at 37°C for 30 minutes and RNA was extracted using the RNeasy mini kit (QIAGEN, Germantown, MD). DNase treatment was done twice for better DNA removal using Qiagen RNase-free DNase during RNA extraction and using NEB DNase after RNA extraction ...
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bioRxiv - Cancer Biology 2021Quote: PBMCs were brought up to 15 mL volume in cold PBS and DNA was isolated with the DNeasy Blood & Tissue Kit (Qiagen catalog # 69504) according to the manufacturer’s protocol with 1 hour of incubation at 55°C for digestion ...
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bioRxiv - Bioengineering 2021Quote: Total RNA content of 3-4 biological replicates of control and samples treated with TNF-α at 40 ng/mL for 24 h were extracted using the RNeasy mini kit (QIAGEN, 74134) according to manufacturer’s instructions ...
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bioRxiv - Microbiology 2021Quote: ... Triton X-100 1.2% and lysozyme 20mg/ml) and incubated at 37°C for 1 h and DNA was extracted with the DNeasy blood&Tissue kit (Qiagen, Courtaboeuf, France) following the manufacturer’s instructions ...
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bioRxiv - Microbiology 2021Quote: ... High-molecular-weight (HMW) genomic DNA from cell pellets of 1.6 ml overnight cultures was extracted using MagAttract® HMW DNA Kit (Qiagen, Hilden, Germany) according to the manufacturer’s instructions and using a final elution volume of 100 μl ...
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bioRxiv - Cell Biology 2021Quote: ... cells were lysed in the plate using 1 mL of RTL buffer from an AllPrep DNA/RNA/Protein isolation kit (Qiagen, Hilden, Germany). Experiments were performed in triplicate.
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bioRxiv - Microbiology 2021Quote: ... DNA was extracted from cultures of LSUCC0096 that had reached max cell density (~106 cells mL−1) growing in JW1 medium (10) at room temperature using a MoBio PowerWater DNA Isolation kit (QIAGEN, Massachusetts, USA) following the manufacturer’s protocols ...
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bioRxiv - Immunology 2020Quote: Dissected specific areas from each three MFPE sections were measured and introduced into RNase-free 0.5 ml Eppendorf tubes with buffer PKD from the miRNeasy FFPE Kit (Qiagen, Valencia, CA, USA). Direct microdissection of IHC stained MFPE sections were attempted but failed to provide RNA with enough quality and yields for further microfluidic quantitative PCR assays.
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bioRxiv - Immunology 2020Quote: Dissected specific areas from each three MFPE sections were measured and introduced into RNase-free 0.5 ml Eppendorf tubes with buffer PKD from the miRNeasy FFPE Kit (Qiagen, Valencia, CA, USA). Direct microdissection of IHC stained MFPE sections were attempted but failed to provide RNA with enough quality and yields for further microfluidic quantitative PCR assays.
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bioRxiv - Microbiology 2023Quote: ... DNA was extracted from spiked blood (1×104 to 1 CFU/mL) using MolYsis Basic5 DNA extraction kit (Molzym, Bremen, Germany) combined with QIAamp UCP Pathogen Mini Kit (Qiagen, Venlo, Netherlands) following manufacturer instructions ...
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bioRxiv - Molecular Biology 2022Quote: ... An aliquot of cells was diluted to OD600 0.3 and 500 µL of diluted cells was added to 1 mL of RNAprotect Bacteria Reagent provided in the RNeasy Mini Kit (Qiagen; catalog #74104). Protocols provided in the “RNAprotect Bacteria Reagent Handbook 2015” were used for RNA extraction (“Protocol 1”) ...
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bioRxiv - Microbiology 2024Quote: Bacterial genomic DNA was extracted from 1 mL overnight cultures grown in LB media using a Qiagen DNeasy Blood and Tissue Kit (Qiagen, Germantown, MD) following the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2023Quote: Total DNA was separately extracted from each 10 mL skinning sample using the DNeasy® mericon® Food Kit (Qiagen, Hilden, Germany). Each extraction was performed following the manufacturer’s instructions for standard large-scale extractions with minor modifications ...
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bioRxiv - Systems Biology 2023Quote: ... for genomic DNA extraction using 1.5 mL of cell culture with a phenol chloroform extraction and phase lock tubes (Qiagen Sciences, Germantown, MD) to enhance phase separation ...
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bioRxiv - Microbiology 2023Quote: Cultured cells were washed once in 10 ml PBS before RNA extraction using the RNeasy Plus Micro kit (Qiagen, Cat. No. 74034). RNA was eluted in 30 µl RNase free H2O and stored at -80◦C until use.
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bioRxiv - Microbiology 2023Quote: ... each Sterivex filter membrane was sectioned in half (representing approximately 500 ml of sample) and placed in Qiagen DNeasy Powersoil DNA isolation kit bead-beating tubes (Qiagen; Hilden, Germany) for immediate DNA extraction and the other half for storage at -80 °C ...
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bioRxiv - Microbiology 2023Quote: DNA was extracted from 1 mL of each human plasma sample using the DNeasy Blood and Tissue kit (Qiagen, 69504, Hilden, Germany) according to the manufacturer protocol and recommendations ...
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bioRxiv - Microbiology 2024Quote: ... The clarified lysate was transferred into the glovebox and loaded onto a gravity column in with 1 mL nickel-nitrilotriacetic acid resin (Qiagen, Hilden, Germany) that was preequilibrated with wash buffer (50 mM Tris-HCl pH 7.5 ...
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bioRxiv - Biophysics 2024Quote: ... The clarified lysate was then applied to a 10 mL bed volume Nickel nitrilotriacetic acid (Ni-NTA) agarose (Qiagen, cat. no. 30230) column ...
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bioRxiv - Molecular Biology 2020Quote: ... about 20 mg of tissue was macerated with sterile blade and digested with 30 μl of Proteinase K (20mg/ml) and 300 μl of ATL buffer (Qiagen Inc., Hilden, Germany) overnight at 56°C ...
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bioRxiv - Genetics 2020Quote: ... we grew one aliquot overnight in 400 mL LB + carbenicillin medium at 30°C and performed three maxi preps and two mega preps (200 mL each) using Qiagen Plasmid Plus Purification kits (Qiagen #12963, #12965, #12981). For the Upstream library ...
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bioRxiv - Neuroscience 2020Quote: ... HSB was removed and beads were re-suspended in 400 µL supplemented RLT buffer (10 µL β-mercaptoethanol/10 mL RLT Buffer) from the RNeasy Plus Micro Kit (Qiagen, Hilden, Germany) and vortexed vigorously ...
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bioRxiv - Cell Biology 2021Quote: ... This was incubated in a 37 °C shaker for 1 hour before the addition of Proteinase K (10 µl, QIAGEN, >600 mAU / ml) and left at 55 °C for 1 hour ...
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bioRxiv - Microbiology 2021Quote: ... Lung homogenates were prepared by homogenizing 200 mg of tissue in a tube containing 1 mL DMEM and a steel bead with a TissueLyser LT (Qiagen, Germantown, MD, USA) for 30 s at 30 hertz ...
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bioRxiv - Neuroscience 2022Quote: PON2 level was quantified in the mouse whole brain homogenate in 100 mg per 1 ml of QIAzol® lysis reagent (Qiagen, Hilden, Germany). mRNA 500 ng was used for cDNA synthesis in 10 μl volume according to the manufacturer’s instructions of PrimeScriptTM RT-PCR kit (Takara Bio Inc. ...
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bioRxiv - Neuroscience 2020Quote: ... HSB was removed and beads were re-suspended in 400 µL supplemented RLT buffer (10 µL β-mercaptoethanol/10 mL RLT Buffer) from the RNeasy Plus Micro Kit (Qiagen, Hilden, Germany) and vortexed vigorously ...
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bioRxiv - Genomics 2023Quote: ... The still frozen leaf powder was transferred into a 2.0 ml Eppendorf tube containing 400 µl API Qiagen extraction buffer (Qiagen DNeasy Plant Mini Kit). The mixture was incubated at 40°C for 1.5 hours with gentle agitation ...
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bioRxiv - Neuroscience 2022Quote: ... Brain tissues were weighted using an analytical scale and homogenized in 1 ml of deionized water using TissueRuptor II electric homogenizer by Qiagen (Germantown, MA, USA). RO5256390 was extracted using acetonitrile/water 50%/50% (v/v ...
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bioRxiv - Microbiology 2023Quote: ... Plant tissue was homogenized in 1.5 ml microtubes with sterile plastic pestles and DNA was extracted using a DNeasy Plant Mini Kit (QIAGEN Sciences Inc., Germantown, MD) following the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2023Quote: ... Resuspend the cells in 10 ml of suspension buffer (10 mM EDTA pH8.0, 150 mM NaCl, 1% glycerol, Lysis blue (1×, from QIAGEN Plasmid Plus Midi Kit), RNase A (0.55 mg/ml) ...
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bioRxiv - Evolutionary Biology 2019Quote: ... Lysates were incubated for 1 hr at 37°C after adding 10 µl of RNAse A (20 mg/ml) and proteins were precipitated with 200 µl of Protein Precipitation Solution (Qiagen Gentra Puregen Cell kit). After centrifugation ...
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bioRxiv - Microbiology 2021Quote: ... Whole ear tissues were homogenised in sterile 1.5 ml flat-bottom tubes containing 400 µl of PowerBead Solution (Part of DNeasy UltraClean Microbial Kit; Qiagen, Cat #12224-250, Hilden, Germany) with an OMNI Tissue Homogeniser with plastic hard-tissue probes (OMNI International) ...
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bioRxiv - Cancer Biology 2022Quote: ... The lysate was cleared by centrifugation (35,000 x g, 1 h) and the supernatant was loaded onto a Ni-affinity chromatography column (Qiagen Ni-NTA Superflow, 20 mL) pre-equilibrated with Ni-NTA buffer ...
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bioRxiv - Microbiology 2023Quote: ... DNA was extracted from a 10 mL culture using the QIAGEN® Genomic DNA Buffer Set and Genomic-tips™ 100/G set (midi-prep) (QIAGEN, Hilden, Germany). This procedure adhered to the Sample Preparation and Lysis Protocol for Bacteria as outlined in the QIAGEN® Genomic DNA Handbook ...
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bioRxiv - Molecular Biology 2020Quote: ... 600 µl buffer RLT plus with 2-Mercaptoethanol (βME) at 10 µl βME/ml of RLT+ was added before placing the tube into a TissueLyser LT (QIAGEN Germantown, MD, USA; Cat#69980) set at 50 oscillations/second for 5 minutes ...
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bioRxiv - Synthetic Biology 2023Quote: ... 25 µL of samples was mixed with Triton X-100 solution and then incubated with 25 µL of Proteinase K (ProK; Qiagen, 19131; 100 µg/mL in PBS) at 37°C for 30 min prior to Tluc measurement.