Labshake search
Citations for Qiagen :
3751 - 3800 of 3900 citations for 4 Methoxy 2 2 3 4 5 5 Hexacb 13C12 99% 50 Ug Ml In Toluene since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Molecular Biology 2022Quote: ... Isolated colonies were inoculated into 5 mL of LB with appropriate antibiotics and plasmids were purified using the alkaline lysis protocol and Qiaquick miniprep kit (Qiagen). Plasmids were verified by sequencing (GATC ...
-
bioRxiv - Biophysics 2022Quote: ... peak fractions were supplemented with 10 mM imidazole and 10 µg/mL aprotinin/leupeptin and bound to NiNTA beads (Qiagen) for 2 hours ...
-
bioRxiv - Genetics 2022Quote: Total RNA was extracted from nearly 100 µl of tightly packed age-synchronized adult worm pellet collected in 1 ml TRIzol reagent provided by Qiagen RNeasy Mini kit (Cat ...
-
bioRxiv - Plant Biology 2022Quote: ... the supernatant was discarded before resuspending the nuclei pellet in 30 mL prewarmed (37 C) G2 lysis buffer (Qiagen, Germany). Resuspended pellets were incubated with RNase A (50 μg/ml ...
-
bioRxiv - Microbiology 2022Quote: ... pH 8.0) containing 1 mg/mL of lysozyme and supplemented with 10 μL of Proteinase K (QIAGEN, cat. no. 19131). The mixture was incubated on a shaker at room temperature for 30 min with intermittent vortexing ...
-
bioRxiv - Microbiology 2022Quote: ... The protein complex was adjusted to 12 mg/ml and screened for crystallization using the 384 conditions of the JCSG Core Suite (Qiagen) on our robotic CrystalMation system (Rigaku ...
-
bioRxiv - Microbiology 2022Quote: ... from 1 mL of culture were frozen at −80°C for later DNA extraction using the DNeasy PowerSoil Kit (Qiagen) according to the manufacturer’s procedure ...
-
bioRxiv - Microbiology 2022Quote: RNA was isolated from approximately 107 cells grown to a density of 106 cells/ml using the RNAeasy Mini Kit (Qiagen), including an on-column DNase I treatment (Qiagen) ...
-
bioRxiv - Neuroscience 2023Quote: ... 1mM DTT and 100 µg/ml cycloheximide) and eluted in 350 µl of RLT Plus buffer from the RNAeasy Plus Micro kit (Qiagen) supplemented with 2-mercaptoethanol (Bio-Rad).
-
bioRxiv - Neuroscience 2022Quote: ... The lysate was centrifuged at 10000g for 10 minutes and the supernatant was allowed to bind with 1 ml of Ni-NTA beads (Qiagen) for 1 hour ...
-
bioRxiv - Microbiology 2022Quote: ... Left lungs were harvested in 2 ml Eppendorf (Stevenage, UK) Safe-Lock micro test tubes containing 500 µl DMEM and a stainless-steel carbide bead (Qiagen), and snap frozen on dry ice until virus titration ...
-
bioRxiv - Microbiology 2022Quote: ... Tissue sections collected for virology were disrupted in 1 mL of sterile PBS using the TissueLyser LT (Qiagen, Germantown, MD) at 30 Hz for 5 min twice ...
-
bioRxiv - Immunology 2022Quote: ... The protein complex was adjusted to 11 mg/ml and screened for crystallization using the 384 conditions of the JCSG Core Suite (Qiagen) on our robotic CrystalMation system (Rigaku ...
-
bioRxiv - Immunology 2022Quote: ... For bacterial load determination one lobe of the lung was collected in 1 mL PBS with 1 metal bead and smashed using a tissue lyser (Qiagen). 25 μL of lysed lung were put in duplicates on CYE-plates and grown for 3-4 days at 37°C in a non-CO2 incubator.
-
bioRxiv - Neuroscience 2024Quote: ... 1mM DTT and 100 µg/ml cycloheximide) and eluted in 350 µl of RLT Plus buffer (RNAeasy Plus Micro kit, Qiagen) supplemented with 2-mercaptoethanol (Bio-Rad).
-
bioRxiv - Synthetic Biology 2024Quote: ... Plasmid DNA was extracted from five 10-mL aliquots of this culture using the QIAprep Spin Miniprep Kit (Qiagen, 27104).
-
bioRxiv - Neuroscience 2024Quote: ... Overnight cultures of the positive clones were grown in LB Kanamycin (30 μg/mL) media and plasmid DNA was extracted using the QIAprep Spin Miniprep Kit (Qiagen). Purified plasmid DNA was sequenced using ABI BigDye Terminator v3.1 Sequencing at the Australian with the following primers ...
-
bioRxiv - Systems Biology 2024Quote: ... and left brain hemisphere using the phenol-chloroform extraction method with 1 mL of Qiazol Lysis Reagent (Qiagen, Hilden, Germany), as previously described in Ederer et al ...
-
bioRxiv - Neuroscience 2024Quote: ... and 20 U/mL RNasin) and resuspended in 100 µL of RLT buffer from the RNeasy Micro Kit (Qiagen 74004) supplemented with 1% β-mercaptoethanol ...
-
bioRxiv - Evolutionary Biology 2024Quote: ... The bits containing the powder was immediately transferred and rinsed in a 2 ml Eppendorf (DNA/RNA and DNase/RNase free tube) containing 1 ml of Tissue Lysis buffer ATL from the QIAamp DNA Micro Kit (Qiagen) and stored at 4°C until the DNA extraction process (i.e ...
-
bioRxiv - Genomics 2024Quote: ... The resulting lysate was then made up to 3 mL with QIAzol Lysis Reagent and mixed thoroughly before 1 mL lysate aliquots were processed using RNeasy Lipid Tissue Kit 74804 (QIAGEN) according to the manufacturer’s instructions ...
-
bioRxiv - Biochemistry 2024Quote: ... RNAse A lysis buffer was prepared from IP buffer but is supplemented with 80 μg/mL of RNase A (Qiagen). Cells were gently resuspended and incubated for 30 minutes rocking on a tube rotator at 4°C ...
-
bioRxiv - Microbiology 2023Quote: During this time a stock of genomic DNA (gDNA) from Escherichia coli (REL606) from ∼72 mL of overnight culture was made using the “DNeasy Ultraclean Microbial kit” (Qiagen), resulting in a stock of ∼30 mL of gDNA at 20 μg/mL (stored at -20 °C) ...
-
bioRxiv - Cancer Biology 2023Quote: ... Proteinase K 20mg/ml was added and incubated at 65 for 1 hour and DNA was purified with PCR purification Kit from Qiagen. Then ...
-
bioRxiv - Microbiology 2023Quote: ... Then 4 μl RNase A (100 mg/ml) were added to the tubes followed by 150 μl of buffer AL (lysis buffer, Qiagen). After mixing by vortexing ...
-
bioRxiv - Microbiology 2022Quote: ... transferred to 2 ml lysis matrix B tubes (MPBio) and subjected to bead beating for 15 min at 30 Hz (Tissuelyser II, Qiagen) with RNAse A added ...
-
bioRxiv - Biochemistry 2023Quote: ... The pellets were resuspended with buffer A and loaded onto a 10 mL column of Ni-NTA agarose (Qiagen, Inc.) equilibrated with buffer A ...
-
bioRxiv - Microbiology 2023Quote: ... The protein concentrations of all the collected samples were adjusted to 400 μg mL−1 using the Qubit protein broad range assay kits (Qiagen). The Cyclic AMP XP® kit ELISA plates were prepared according to the manufacturer’s protocols ...
-
bioRxiv - Biophysics 2023Quote: ... We grew sequence-positive clones for the plasmid extraction at 37°C overnight in presence of a selective antibiotic Chloramphenicol (30 µg/ml) and isolated the final plasmid using a QIAprep Spin Miniprep kit (Qiagen) the following day ...
-
bioRxiv - Biophysics 2023Quote: ... We grew sequence-positive clones for the plasmid extraction at 30°C overnight in presence of a selective antibiotic Ampicillin (100 µg/ml) and isolated the final plasmid via a Midiprep using a Qiafilater plasmid midi kit (Qiagen) the following day.
-
bioRxiv - Microbiology 2023Quote: DNA was extracted from enrichment material in technical duplicates (1 ml digestate or 0.25 g soil) using the PowerLyzer™ Soil DNA extraction kit (QIAGEN) with modified bead beating using a MP Biomedicals™ FastPrep®-24 (Thermo Fischer Scientific Inc ...
-
bioRxiv - Microbiology 2023Quote: ... We could not get a PCR product for the R escaper and therefore extracted the total DNA from the escaper and the ancestor λ-vir phage lysate after enrichment for high phage titer (> 107 pfu/ mL) DNA with the DNeasy blood and tissue kit (QIAGEN), starting from the Proteinase-K treatment step to lyse the phages 92 ...
-
bioRxiv - Molecular Biology 2023Quote: The libraries were purified with a 30-minute incubation at 37 °C in the presence of 160 μg/mL RNase A (Qiagen), a treatment with 200 μg/mL Proteinase K (Qiagen ...
-
bioRxiv - Cancer Biology 2023Quote: ... a ∼20 mg chunk of frozen lung tissue was collected using a scalpel and placed in 1 mL RLT buffer (Qiagen) with 1:100 β-mercaptoethanol in a Precellys silicone bead dissociation tube (Bertin-Corp) ...
-
bioRxiv - Immunology 2023Quote: ... Cells were stimulated with LPS (1 μg/ml) for 6 hours at 37°C followed by RNA isolation using a RNeasy Kit (QIAGEN). Extracted RNA was converted to cDNA with an iScript cDNA Synthesis Kit (Bio-Rad) ...
-
bioRxiv - Neuroscience 2023Quote: ... brain hemispheres were homogenized in freshly prepared lysis buffer (0.6 ml per 30 mg of brain tissue) containing 1% β-mercaptoethanol using a TissueRuptor homogenizer (Qiagen).
-
bioRxiv - Physiology 2023Quote: ... were immersed in the Transnetyx microbiome sample collection tube (1.5 mL, prefilled with DNA stabilization liquid and glass beads) and sent to Transnetyx (Cordova, TN) for automated DNA extraction (Qiagen DNeasy 96 PowerSoil Pro QIAcube HT extraction kit) ...
-
bioRxiv - Immunology 2023Quote: ... Plasmid DNA from overnight liquid cultures (LB with 100 mg/ mL ampicillin) was isolated with QIAprep Spin Miniprep Kit (Qiagen).
-
bioRxiv - Synthetic Biology 2023Quote: ... The organs were weighed and lysed in 1 mL Triton X-100 solution (0.1% in PBS) using a TissueLyser II (QIAGEN, Germany) according to the manufacturer protocol ...
-
bioRxiv - Cancer Biology 2023Quote: Investigation of the cell cycle related genes in LoVo cell line on treatment with FA FOS I was performed as described above by using the synchronized and 24 h FA FOS I (198 µg/ml) treated cells profiled by using RT2 Prolifer PCR Array- Human Cell cycle from Qiagen. The data was analysed by using Gene globe platform and reactome pathway analysis.
-
bioRxiv - Genetics 2023Quote: ... Cells were washed three times with ice-cold PBS and lysed at room temperature with 1 ml/well QIAzol lysis reagent (QIAGEN). Lysates were incubated at room temperature for 5 min and homogenised by pipetting up and down ...
-
bioRxiv - Bioengineering 2023Quote: ... The supernatants were then supplemented with 20 mM imidazole and then loaded onto 1 mL bed volume of Ni-NTA agarose beads (Qiagen) that were pre-washed with 5 column volumes of 1x PBS ...
-
bioRxiv - Genomics 2023Quote: ... After ligation nuclei were pelleted and resuspended in cold PBS with DAPI to a final concentration of 300nM and GFP+ cells were FAC-sorted into a 96 well plate with 2ul lysis buffer (20mM Tris pH 8, 20mM NaCl, 0.15% Triton X-100, 25mM DTT, 1mM EDTA, 500nM Carrier ssDNA, and 15ug/mL Qiagen Protease) and lysed for 1 hour at 50°C and inactivated 15 minutes at 70°C ...
-
bioRxiv - Genomics 2023Quote: ... then stopped with transposome removal buffer (300nM NaCl, 45 mM EDTA, 0.01% Triton X-100 with 100ug/mL Qiagen Protease) and incubated at 50°C for 40 minutes and 70°C for 20 minutes ...
-
bioRxiv - Developmental Biology 2023Quote: ... we used 6 mL of 55 °C Cell Lysis Solution from The Gentra Puregene® Cell and Tissue Kit (Qiagen) with 0.1 mg/mL proteinase K and 1% β-mercaptoethanol ...
-
bioRxiv - Microbiology 2023Quote: ... was added to the supernatant to give a final concentration of 1 mM and the resultant suspension was applied to a 1 ml Ni2+-NTA column (Qiagen). The resin was then washed sequentially with 10 ml of lysis buffer (without Triton X-100) ...
-
bioRxiv - Genomics 2023Quote: ... tubes were incubated at 56°C for 1 hour followed by addition of 2ul of RNAse A (100mg/ml, QIAGEN) to 1ml of saliva sample and incubated at room temperature for 5 minutes ...
-
bioRxiv - Microbiology 2023Quote: ... RpaB was purified following the same protocol39 using Precellys 24 (Bertin Technologies) to disrupt the cells and 0.5 mL of bedded Ni-NTA agarose beads (Qiagen GmbH) to bind 6×His-tagged proteins ...
-
bioRxiv - Molecular Biology 2023Quote: ... Resulting DNA pellets were washed with 1.0 mL 70% ice-cold ethanol and re-suspended in 30 µL of EB buffer (Qiagen, Germany) after drying.
-
bioRxiv - Biochemistry 2023Quote: ... about 100 mg of tissue samples were homogenised in 1 mL of TRI reagent at room temperature using a tissue lyser (Qiagen). The homogenised samples were transferred to fresh microcentrifuge tubes for RNA isolation ...