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Citations for Qiagen :
3501 - 3550 of 6247 citations for hsa mir 150 3p RT PCR Primer Set since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Developmental Biology 2023Quote: ... and purification with QIAquick PCR Purification Kit (28104, Qiagen). DNA levels in input and immunoprecipitated solutions were determined by qPCR and fold enrichments was calculated as the ration between the input and the immunoprecipitated DNAs ...
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bioRxiv - Genomics 2023Quote: ... DNA was purified using Qiagen PCR kit (Qiagen 28104). ChIP DNA libraries were made using Ovation® Ultralow System V2 kit (Tecan Genomics 0344NB-32 ...
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bioRxiv - Developmental Biology 2023Quote: ... Amplicons were purified by QIAquick PCR purification kit (Qiagen) and submitted for Amplicon-EZ Illumina-based sequencing by Azenta/Genewiz (New Jersey ...
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bioRxiv - Microbiology 2023Quote: ... ARGs were amplified using a multiplex PCR kit (Qiagen) with the following reaction conditions ...
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bioRxiv - Genetics 2023Quote: ... followed by purification using QIAquick PCR Purification Kit (Qiagen). Samples were further purified by 2% E-Gel SizeSelect agarose gel (Invitrogen) ...
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bioRxiv - Biochemistry 2023Quote: ... PCR products were purified (gel extraction; Qiagen, Hilden, Germany), incubated with Dpn I (New England Biolabs Inc. ...
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bioRxiv - Molecular Biology 2023Quote: ... Amplified libraries were purified with PCR cleanup kit (Qiagen) and library quality was assessed on a Tapestation (Agilent Technologies) ...
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bioRxiv - Microbiology 2023Quote: ... 6.5 µL of PCR grade nuclease-free water (Qiagen), and 10 µL of 2x Platinum Hot Start DNA Polymerase (Thermo Fisher Scientific ...
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bioRxiv - Molecular Biology 2023Quote: ... the PCR products were purified using MinElute columns (Qiagen) and eluted in 30 µl elution buffer (EB) ...
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bioRxiv - Molecular Biology 2023Quote: ... Each library was cleaned with PCR purification kit (Qiagen) and eluted with 30 μL of RNase-free water ...
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bioRxiv - Microbiology 2023Quote: ... selected genomic regions were PCR amplified and cleaned (Qiagen) and sent for sequencing to the Dartmouth College Genomics and Molecular Biology Shared Resources Core ...
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bioRxiv - Cancer Biology 2023Quote: ... PCR products were gel extracted using spin columns (Qiagen) and sequenced ...
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bioRxiv - Cell Biology 2023Quote: ... DNA was extracted with QIAquick PCR purification kit (Qiagen). DNA elution was used in qPCR analysis to measure the abundance of specific DNA sequences.
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bioRxiv - Microbiology 2023Quote: ... before purification with the QIAquick PCR purification kit (Qiagen). Reactions were performed in technical triplicates in a MasterCycler RealPlex (Eppendorf ...
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bioRxiv - Genomics 2024Quote: ... purified with the MinElute PCR Purification Kit (Qiagen, 28004), and quantified using a Bioanalyzer High-Sensitivity DNA Assay (Agilent Technologies ...
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bioRxiv - Cancer Biology 2024Quote: ... DNA was purified using QIAquick PCR Purification Kit (Qiagen). The experiments were performed in biological duplicate and the data were presented as mean ± SEM ...
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bioRxiv - Biophysics 2024Quote: ... All PCR products were cleaned up (Qiagen, Cat# 28104) prior to use.
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bioRxiv - Bioengineering 2024Quote: ... with QuantiTect SYBR Green PCR Kit (QIAGEN Cat#204145). Expression of genes in the measurement of arbitrary units was calculated relative to RN18S using the following equation and reported in bar graphs for each gene analyzed ...
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bioRxiv - Immunology 2024Quote: ... DNA was purified with MinElute PCR Purification Kit (QIAGEN). Tagmented DNA was amplified with Kapa Hifi Hot Start Kit (Kapa Biosystems ...
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bioRxiv - Cancer Biology 2024Quote: ... cleaned up with a PCR purification kit (#28104, Qiagen) and ligated into the cut LT3GEPIR vector with T4 DNA Ligase at a 3:1 insert ...
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bioRxiv - Neuroscience 2024Quote: ... DNA was purified by QIAquick PCR Purification Kit (Qiagen) and quantified by real-time qPCR ...
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bioRxiv - Cell Biology 2024Quote: ... followed by a QIAGEN PCR cleanup kit (Qiagen, #28106). The libraries were then sequenced on NovaSeq (Illumina ...
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bioRxiv - Immunology 2024Quote: ... RT2 Profiler PCR Array Human Inflammatory Response & Autoimmunity (Qiagen) was used according to the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2024Quote: ... purified by MinElute 96 UF PCR Purification Kit (Qiagen), eluted in 50 µL pure water and quantified using the Qubit dsDNA broad range (BR ...
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bioRxiv - Molecular Biology 2024Quote: ... PCR products were visualized via capillary electrophoresis (QIAxcel, QIAGEN) and then purified and normalized via an NGS Normalization 96-Well Kit (Norgen Biotek Corporation) ...
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bioRxiv - Genomics 2024Quote: ... DNA was purified using QIAquick PCR purification kit (Qiagen). The recovered DNA was subjected to library construction using NEBNext Ultra DNA library prep kit (NEB) ...
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bioRxiv - Pathology 2024Quote: PCR products were purified using Qiaquick purification kit (Qiagen) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2024Quote: ... using QuantiTect multiplex PCR mix (Qiagen, Germantown, MD, USA), using the following PCR cycling conditions ...
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bioRxiv - Cell Biology 2024Quote: ... the PCR product gel purified (Gel Extraction Kit, Qiagen) and Sanger-sequenced (Retrogen) ...
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bioRxiv - Microbiology 2021Quote: Total RNA isolated from HUVEC was used for miRNA quantification using the miRCURY™ LNA™ miRNA RT Kit (Qiagen). Briefly ...
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bioRxiv - Microbiology 2019Quote: ... The three MDA product replicates were then pooled together with 17 µl of the remaining un-amplified RT product and purified using DNeasy Blood & Tissue kit (QIAGEN). Concentration of DNA was determined using the Qubit dsDNA HS kit and the Qubit 3 fluorometer (Invitrogen/ThermoFisher Scientific) ...
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bioRxiv - Bioengineering 2020Quote: ... 100 ng equivalent of pooled total RNA (5 slices per animal; 7 rats) was used to synthesize cDNA using an RT First Strand Kit (Qiagen). Pre-validated primers targeting rat CXCR4 ...
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bioRxiv - Molecular Biology 2020Quote: ... cDNA was synthesized using the miScript II RT Kit with Hiflex buffer from 12 μL of extracted total RNA according to product manual (Qiagen). Expression of mRNA was quantified using QuantiTect SYBR Green PCR kit (Qiagen ...
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bioRxiv - Plant Biology 2021Quote: The total RNA used for RT-qPCR analysis was isolated from rice or Arabidopsis tissues using an RNeasy system (Qiagen) following the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2022Quote: ... Real-time qPCR reactions were run using the MESA Green qPCR MasterMix Plus for SYBR Green assay (Eurogenetec, RT-SY2X-03+WOU) on the Rotor-Gen Q device (Qiagen). Each sample was tested in triplicate ...
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bioRxiv - Systems Biology 2022Quote: ... Real-time qPCR reactions were run using the MESA Green pPCR MasterMix Plus for SYBR Green assay (Eurogenetec, RT-SY2X-03+WOU) on the Rotor-Gen Q device (Qiagen). Each sample was tested in triplicate ...
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bioRxiv - Neuroscience 2021Quote: Two-step reverse transcription qPCR (RT-qPCR) was performed by first converting total RNA into cDNA via QuantiTect Reverse Transcription Kit (Qiagen). Resulting cDNA was queried for expression of genes of interest and the 18s reference gene via qPCR using TaqMan probes (Applied Biosystems ...
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bioRxiv - Microbiology 2020Quote: ... buffer with lysozyme (1mg/mL) and incubated at RT for 2 min prior to adding 700 μL RLT Buffer (Qiagen) with 10ul/mL B-mercaptoethanol (Sigma) ...
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bioRxiv - Molecular Biology 2020Quote: ... cDNA was synthesized using 200ng RNA and the miScript II RT Kit according to the manufacturer’s protocol (Qiagen, Crawley, UK). qPCR mastermix was prepared using the miScript SYBR Green PCR Kit (Qiagen ...
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bioRxiv - Plant Biology 2019Quote: ... cDNA was synthesised from the total RNA using the Qiagen Quantitect RT kit according to the manufacturer’s instructions (Qiagen, Germany). The resulting cDNA was diluted 1:4 with molecular biology grade water prior to use in RT-qPCR ...
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bioRxiv - Molecular Biology 2019Quote: ... 1pM RT4 was added to the Qiazol before RNA extraction as internal control and 5 uL of total RNA was used as input for reverse transcription reactions using the miScript RT II System (Qiagen) followed by QuantiTec SYBR Green PCR kit (Qiagen) ...
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bioRxiv - Molecular Biology 2021Quote: We performed RT-qPCR, using the AceQ qPCR SYBR Green Master Mix (Q111-03, Vazyme) on the thermocyclers Rotor-Gene Q (Qiagen) or LightCycler 96 thermal cycler Instrument (Roche Applied Science ...
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bioRxiv - Microbiology 2020Quote: ... EAV genome copy number was determined by one step RT-qPCR using the QuantiTect™ Virus + ROX Vial Kit (Qiagen). Each RNA sample was tested in triplicate ...
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bioRxiv - Neuroscience 2019Quote: ... cDNA libraries of miRNAs were created from 20ng of total RNA with the mirCURY LNA RT Kit (Qiagen, Germantown, MD). PCR reactions were performed using the miRCURY LNA SYBR Green PCR Kit and the following locked nucleic acid (LNA ...
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bioRxiv - Cell Biology 2020Quote: ... The RNA concentration was measured using Geneflow Nanophotometer and RT-qPCR was performed with one-step QuantiFast SYBR Green qPCR kit (Qiagen) using 50ng of RNA in each sample ...
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bioRxiv - Cell Biology 2020Quote: ... samples were centrifuged for 10 minutes at 20000 g at RT and pellets obtained pellets were resuspended in 10 μl of EB (Qiagen) shaking at 55°C for 10 minutes and finally purified with a PCR purification kit ...
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bioRxiv - Microbiology 2021Quote: ... A small portion of the RNA was then reverse transcribed into cDNA using the miScript II RT kit (Qiagen 218160) followed by qPCR ...
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bioRxiv - Plant Biology 2022Quote: ... RT-qPCR reactions were performed in triplicate with a Qiagen Rotor-Gene Q 5Plex real-time cycler (Qiagen, Germantown, MD). Transcript levels of each gene were calculated relative to that of PP2A ...
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bioRxiv - Physiology 2023Quote: ... small RNA fragment enriched total RNA isolated from each sample was reverse transcribed into cDNA using a miScript II RT Kit (Qiagen). RT-qPCR was performed using miScriptSYBR Green PCR Kit (Qiagen ...
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bioRxiv - Cancer Biology 2024Quote: ... A total of 1 μg of RNA was used as input for cDNA synthesis reactions by using the Omniscript RT Kit (Qiagen) along with random hexamers ...