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Citations for Qiagen :
3451 - 3500 of 3857 citations for 6 Chloro 4 hydroxy 2 methyl 2H thieno 2 3 e 1 2 thiazine 3 carboxylic acid methyl ester 1 1 dioxide since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2023Quote: ... Expressed proteins were harvested by centrifugation 6 days post transfection and immediately purified from the supernatant on a Ni-NTA Superflow column (#30430, Qiagen). Eluted protein fraction was buffer exchanged into PBS pH 7.4 using a HiPrep 26/10 Desalting column (#GE17-5087-01 ...
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bioRxiv - Microbiology 2023Quote: ... cells were grown in TSB for 6 hr and total bacterial RNA was isolated using the RNeasy mini kit (Qiagen) with on-column DNA digestion according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2023Quote: ... RNA was harvested at 6 and 24 h post IFN-treatment (three independent biological triplicates) and isolated using the RNeasy Mini Kit (Qiagen). RNA-Seq was performed by Novogene using a PE150 sequencing strategy on the Illumina NovaSeq 6000 platform ...
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bioRxiv - Molecular Biology 2023Quote: Total RNA was extracted from ∼70% confluent HeLa cells grown in 6-wells plate using a RNeasy Micro Kit (Qiagen) with a DNase step according to the manufacturer’s instructions ...
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bioRxiv - Plant Biology 2022Quote: RNA was isolated from 6-day old Arabidopsis plants using the QIAGEN RNeasy Mini Kit (ID: 74104, QIAGEN, Hilden, Germany). RNA quality was determined using a Nanodrop 2000/2000c spectrophotometer (Thermo Fisher Scientific ...
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bioRxiv - Cancer Biology 2023Quote: Total RNA was extracted from FACS-sorted L-GMPs from BL/6 and Il21-/- AML mice using the RNeasy Micro Kit (Qiagen). RNA purity was checked using the NanoPhotometer® spectrophotometer (Implen) ...
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bioRxiv - Systems Biology 2023Quote: ... DAPT was washed out and cells were allowed to signal for 6 hours before cells were harvested for RNA extraction using QIAshredder columns (Qiagen) and the RNeasy Mini Kit per the manufacturer’s instructions (see next section for further details of the plated ligand assay) ...
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bioRxiv - Biochemistry 2022Quote: ... complexes were either concentrated using a 50 kDa MWCO concentrator and further purified on a Superose 6 Increase 10/300 column (Cytiva) equilibrated in low-salt CSFxB or loaded directly onto Ni2+ agarose (Qiagen), eluted using 300 mM imidazole ...
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bioRxiv - Cell Biology 2023Quote: ... HEK293T cells from single wells of a 6-well plate were pelleted and total RNA was extracted using the RNeasy MiniKit (QIAGEN), following the manufacturer’s instructions ...
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bioRxiv - Genomics 2023Quote: ... Swabs were incubated at 37 degrees Celsius for 30 minutes then bead beaten for 6 minutes at 30Hz in a TissueLyser II (Qiagen). The rest of the protocol was followed increasing the volumes to account for the additional lysis buffer ...
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bioRxiv - Evolutionary Biology 2023Quote: We extracted total RNA from liver and BAT from each sample (n = ∼6 per genotype/sex/treatment/tissue) using the RNeasy PowerLyzer Kit (QIAGEN). We generated Illumina cDNA libraries from 1 μg of purified RNA using KAPA Stranded mRNA-Seq Kit (Illumina) ...
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bioRxiv - Developmental Biology 2023Quote: ... we used 6 mL of 55 °C Cell Lysis Solution from The Gentra Puregene® Cell and Tissue Kit (Qiagen) with 0.1 mg/mL proteinase K and 1% β-mercaptoethanol ...
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bioRxiv - Biochemistry 2023Quote: ... Proteins harvested after 6 days post transfection were purified from the supernatant on a Ni-NTA Superflow column (#30430, Qiagen). Immediately after the elution ...
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bioRxiv - Neuroscience 2023Quote: Monolayer of 1.0 × 106 cells grown in 6 well plate was lysed in 500 μl/well QIAzol Lysis Reagent (Qiagen #79306) and RNA extraction was performed with QIAzol miRNeasy Mini Kit (Qiagen #217004 ...
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bioRxiv - Cell Biology 2024Quote: ... Azure Cielo 6 (Azure Biosystem) was used to detect the signal of mRNA expression level with QuiantiNova SYBR Green (Qiagen). Target genes included ZEB1 and FN1 for mesenchymal epithelial transition markers and TEAD1 and CTGF for Yap-associated markers ...
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bioRxiv - Immunology 2024Quote: BMDM treated in 6-well plates were washed twice with PBS before total RNA purification using the RNeasy kit (Qiagen) following the manufacturer’s recommendations ...
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bioRxiv - Cancer Biology 2024Quote: RNA was extracted from cell lines grown in 6-well plates or from cell pellets using the RNAeasy Mini Kit (Qiagen) as per the manufacturer’s instructions ...
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Braf-mutant Schwann cells divert to a repair phenotype to induce congenital demyelinating neuropathybioRxiv - Developmental Biology 2024Quote: ... at 6 m/s for 50 sec and total RNA extracted with the RNeasy Lipid Tissue Mini Kit (#74804; Qiagen). For SCP (D18 ...
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bioRxiv - Microbiology 2024Quote: DNA extraction from cultured treponemes propagated in 6-well plates following transformation and expansion was performed using the QIAamp DNA mini kit (Qiagen) according to the manufacturer’s instructions ...
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bioRxiv - Genomics 2022Quote: ... Nucleic acids (DNA/RNA) were isolated from flash-frozen tissues using the All Prep DNA/RNA Mini Kit (Qiagen, Germantown, MD) as previously described [127–129] ...
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bioRxiv - Neuroscience 2019Quote: RNA was isolated from vehicle- or bile acid-treated microglia and astrocyte cultures using RNeasy Plus Mini Kit (Qiagen, Hilden, Germany). First-strand cDNA was synthesized by reverse transcription using the iScript cDNA Synthesis Kit (Bio-Rad ...
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bioRxiv - Neuroscience 2021Quote: ... double-digoxigenin locked nucleic acid probe (RNO-MIR-124-3P: CATTCACCGCGTGCCTTA, Tm: 84°C, 339111 YD00614870-BGC, miRCURY LNA™ miRNA Detection Probe, Qiagen) was used at a final concentration of 30 nM ...
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bioRxiv - Cancer Biology 2020Quote: ... cells were washed twice in PBS and incubated for 20 min at 54 °C (60 min at 52 °C for tissue sections) in mercury locked-nucleid acid (LNA) miRNA ISH Buffer 80 nM hybridiziation buffer (100 nM for tissue section) (Qiagen, 339450). Digoxigenin labeled miRCURY LNA probes (HSA-MIR-10B-5P ...
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bioRxiv - Microbiology 2021Quote: Samples were homogenized and nucleic acids were extracted using the MoBio RNA PowerSoil® Total RNA Isolation Kit (Qiagen, CA, USA) and the MoBio RNA PowerSoil® DNA Elution Accessory Kit (Qiagen ...
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bioRxiv - Cancer Biology 2020Quote: Frozen tissues were weighed and homogenized in RLT and nucleic acids were extracted using the AllPrep DNA/RNA Mini Kit (QIAGEN, #80204) according to the manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2021Quote: ... Total RNA was isolated using the RecoverAll Total Nucleic Acid Isolation Kit (Thermo #AM1975) and concentrated using the RNeasy MinElute Cleanup Kit (Qiagen #74204). Library preparation and sequencing were performed by the Genome Sequencing Facility at St ...
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bioRxiv - Molecular Biology 2020Quote: ... The supernatant was divided into two parts: one part was directly combined with nickel-nitrilotriacetic acid (Ni-NTA) affinity resin (Qiagen, China); the other part was heated in a 65 °C water bath for 60 minutes and then centrifuged at 14000 rpm for 60 minutes ...
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bioRxiv - Immunology 2021Quote: ... The plasmid was transfected to 293T cells and the recombinant S protein trimers were purified by Ni-NTA (nickel-nitrilotriacetic acid) chromatography (QIAGEN, Germany), followed by size exclusion to further purify the trimers ...
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bioRxiv - Microbiology 2022Quote: ... nucleic acid extractions were performed by combining equal amounts of cell culture supernatants with RLT Lysis Buffer (Qiagen, Germantown, MA, USA), with 200 µL of the lysate used for magnetic bead-based extraction according to the manufacturer’s protocol ...
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bioRxiv - Biochemistry 2023Quote: ... The supernatant was adjusted to 500 mM NaCl and 20 mM imidazole before affinity purification by incubation with nickel-nitrilotriacetic acid (Ni-NTA) resin (Qiagen, 30210) for 90 min at 4 °C ...
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bioRxiv - Biochemistry 2022Quote: Lysates of His-tagged α-catenin WT and Δmod were bound to Ni-NTA (Ni2+-nitrilotriacetic acid)-sepharose affinity chromatography (Qiagen), washed with 50 mM Tris pH 7.8 ...
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Macrodomain Mac1 of SARS-CoV-2 Nonstructural Protein 3 Hydrolyzes Diverse ADP-ribosylated SubstratesbioRxiv - Biochemistry 2023Quote: ... Frozen cells were thawed on ice and used for protein purification with nickel-nitrilotriacetic acid (Ni-NTA) Fast Start column as recommended by the manufacturer (Qiagen, Germany). The bound proteins were eluted from the column with elution buffer containing 50 mM NaH2PO4 ...
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First complete genome characterization of an Indian pigeon pox virus directly from a clinical samplebioRxiv - Evolutionary Biology 2023Quote: ... The extraction of nucleic acid from infected scab samples was performed by using DNeasy blood and tissue purification Kits (QIAGEN, USA) with some modification established by Sarker et al. ...
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bioRxiv - Neuroscience 2024Quote: Ribonucleic acid (RNA) was purified from the dissected ventral mesencephalon containing SNpr using RNeasy Microarray Tissue Kit (Qiagen, Copenhagen, Denmark, DK) with an on-column deoxyribonuclease (DNase ...
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bioRxiv - Biochemistry 2024Quote: ... the EcNhaA triple mutant was extracted from membranes with n-Dodecyl β-D-maltoside (DDM; Glycon) and purified by Ni-nitrilotriacetic acid (Ni-NTA; Qiagen) affinity chromatography ...
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bioRxiv - Molecular Biology 2024Quote: ... cells in each well were transfected with 50 μM of each ASO locked nucleic acid GapmeR1 or GapmeR2 (Qiagen, custom design) with Negative Control GapmeR A (30300019-2 ...
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bioRxiv - Developmental Biology 2021Quote: ... Lysates were treated with 4 μl of 100 mg/ml RNase A (QIAGEN) at 37 °C shaking at 500 RPM for 3 hours ...
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bioRxiv - Immunology 2021Quote: ... and kept at 4°C RNA was purified using RNEASY Mini Kit (Qiagen) and its concentration determined using a Nanodrop 1000 ...
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bioRxiv - Neuroscience 2021Quote: ... 4°C for 10 min and the pellet was lysed with RLT+ (Qiagen). For scRNA-sequencing ...
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bioRxiv - Microbiology 2021Quote: ... at 4°C until RNA was extracted using the RNeasy Mini Kit (Qiagen)
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bioRxiv - Microbiology 2020Quote: The MoBio PowerSoil-htp 96 kit (now Qiagen Cat No./Id: 12955-4), with minor modifications ...
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bioRxiv - Cancer Biology 2020Quote: ... cell pellets were lysed in a 4% SDS buffer using a Qiashredder (Qiagen). Relative densitometry for western blots was determined using ImageJ software and normalized to the density of loading control β-ACTIN ...
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bioRxiv - Microbiology 2021Quote: ... into a volume of 4 μL PBS sc 1X (Qiagen, Cat. No. 150345). To avoid evaporation of the PBS sc 1X during the sort ...
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bioRxiv - Cell Biology 2019Quote: J774 cells were silenced using a pool of 4 different Flexitube siRNAs (Qiagen) for SYK with Lipofectamine RNAiMAX reagent as described previously 20 ...
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bioRxiv - Genomics 2021Quote: ... 4 μL of room temperature Stop solution (REPLI-g Single Cell Kit, Qiagen) was then added and the samples were vortexed and spun down ...
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bioRxiv - Molecular Biology 2023Quote: ... Effectene (4 μL of enhancer and 5 μL of Effectene reagent; Qiagen 301427) was used to transfect 500 ng of the indicated eGFP plasmid ...
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bioRxiv - Synthetic Biology 2022Quote: ... 18 µl of Omniscript enzyme at 4 U/µl (Omniscript RT Kit, Qiagen), and 37 µl water ...
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bioRxiv - Evolutionary Biology 2023Quote: ... Crystals were grown at 4 °C in EasyXtal-15-Well Tools plates (Qiagen), using hanging-drop vapour diffusion ...
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bioRxiv - Cell Biology 2023Quote: ... cells were transfected with dsHsc70-4 with the Effectene Transfection reagent (301427, QIAGEN) and incubated for 72 h ...
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bioRxiv - Biophysics 2022Quote: ... The supernatant was incubated overnight at 4 °C with Strep-Tactin Beads (Qiagen), typically using 0.75 ml packed beads per two liters of original culture volume ...