Labshake search
Citations for Qiagen :
3451 - 3500 of 10000+ citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Bioengineering 2024Quote: ... followed by purification with a RNeasy Mini Kit (Qiagen) according to the manufacturer’s protocol ...
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bioRxiv - Biochemistry 2024Quote: ... The supernatant was incubated with Ni-nitrilotriacetic acid (Ni-NTA) resin (Qiagen, Germany) on a rocker for 1 hour at 4 °C ...
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bioRxiv - Biochemistry 2024Quote: ... RNA was extracted using the RNeasy Mini Kit (Qiagen) and processed according to the Illumina TruSeq RNA Sample Prep Kit ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... Allele-specific expression was measured using RT-ddPCR with the One-Step RT-ddPCR Advanced Kit for Probes (Qiagen) on a QX200 ddPCR Droplet Reader (BioRad ...
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bioRxiv - Developmental Biology 2024Quote: ... we used the RNeasy Mini Kit (Qiagen 74104) to isolate RNA and the High-Capacity RNA-to-cDNA Kit (Applied Biosystems 4387406 ...
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bioRxiv - Neuroscience 2024Quote: ... MinElute PCR Purification Kit buffer PB (Qiagen) was added to stop the reaction and tagmented DNA was purified and eluted in 10 μl of EB ...
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bioRxiv - Biochemistry 2024Quote: ... v5.2.1 (Qiagen) was used to assemble processed sequences into contigs and ORFs were identified by FragGeneScan ...
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bioRxiv - Biochemistry 2024Quote: ... v5.2.1 (Qiagen) to generate mapped raw read counts for each ORF ...
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bioRxiv - Microbiology 2024Quote: ... viral RNA was extracted from 50 ml cell culture medium using QIAamp Viral RNA Mini Kit according to protocol provided by the manufacturer (Qiagen, Cat. no. 52904). RNA concentration and quality were evaluated using NanoDrop 2000 Spectrophotometer (Thermo Fischer Scientific ...
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bioRxiv - Immunology 2024Quote: ... with DNase treatment (Cat# 79254; Qiagen). RNA concentration was measured with a Qubit RNA HS Assay Kit (Cat# #Q32855 ...
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bioRxiv - Cancer Biology 2024Quote: ... RNA extraction was performed using RNeasy mini kit from Qiagen (catalog number 74106).
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bioRxiv - Cancer Biology 2024Quote: ... Genomic DNA was extracted using the QIAamp Blood Maxi Kit (Qiagen, Hilden, Germany). Two polymerase chain reactions (PCRs ...
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bioRxiv - Cancer Biology 2024Quote: ... Plasmid DNA was prepared (Qiagen, 27104) and sequenced from T7 primer by Sanger sequencing.
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bioRxiv - Cell Biology 2024Quote: ... p.H 8.0 (Qiagen 12162) to a total volume of 250µl ...
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bioRxiv - Cell Biology 2024Quote: Total RNA was extracted using the RNeasy mini kit (Qiagen). Library preparation was carried out by the Edinburgh Clinical Research Facility from 500 ng of each RNA sample using the NEBNext Ultra II Directional RNA library kit with PolyA enrichment module (New England Biolabs) ...
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bioRxiv - Cell Biology 2024Quote: ... The DNAs were purified using the MinElute PCR Purification Kit (Qiagen).
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bioRxiv - Cell Biology 2024Quote: ... cut out from the gel and purified using QIAquick gel extraction kit (Qiagen).
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bioRxiv - Cell Biology 2024Quote: ... cut out from the gel with blade and tweezer and purified using QIAquick gel extraction kit (Qiagen). ° PAUSE POINT PCR fragments can be stored at −20 °C up to several months
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Development of a genetically encoded sensor for probing endogenous nociceptin opioid peptide releasebioRxiv - Neuroscience 2024Quote: ... HEK293T cells were transfected with Effectene transfection kit (QIAGEN). 24 hours after transfection cells were disscociated with Versene (Thermo Fisher ...
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bioRxiv - Microbiology 2024Quote: ... No siRNAs were added to mock samples and 50 pmol of scrambled siRNA (SI03650318; QIAGEN) was used as a negative control ...
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bioRxiv - Systems Biology 2024Quote: ... were transfected with one of three different commercial siRNA sequences designed to target the EGFR or an Allstars negative control siRNA sequence (final concentration 1 nM; all Qiagen Ltd., UK) using INTERFERin transfection reagent according to the manufacturer’s instructions (Polyplus Transfection ...
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bioRxiv - Neuroscience 2024Quote: ... RNA samples were extracted from 1 million cells using the RNeasy Mini Kit (Qiagen, 74136) and treated with TURBO DNA-freeTM Kit (Thermo Fisher Scientific ...
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bioRxiv - Synthetic Biology 2024Quote: ... and either Mini-prepped (using a Qiagen Mini-prep kit) or Midi-prepped (using a Macharey-Nagel NucleoBond Xtra Midi kit ...
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bioRxiv - Synthetic Biology 2024Quote: ... The supernatant was applied to a Ni-NTA agarose drip column (Qiagen) and eluted with buffer A supplemented with 250 mM imidazole ...
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bioRxiv - Neuroscience 2024Quote: ... These samples were subjected to RNA extraction using the RNeasy Plus Mini kit (Qiagen, 74136). Library preparation (polyA-selected ...
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bioRxiv - Developmental Biology 2024Quote: ... RNA was then extracted using the RNeasy Mini Kit (Qiagen, Hilden, Germany). RNA quality was assessed using a NanoDrop 2000 Spectrophotometer (Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2024Quote: Total RNA was extracted using the RNeasy Mini Kit (Qiagen) following the manufacturer’s protocol ...
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bioRxiv - Developmental Biology 2024Quote: ... Reverse transcription was then performed using QuantiTect reverse transcription kit (Qiagen). The qPCR assays for detecting KLF4 (Mr04421256_mr) ...
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bioRxiv - Cancer Biology 2024Quote: ... The band containing the construct (13,200 bp) was gel-purified (0.8% agarose gel) using the QIAquick Gel Extraction Kit (#28704, Qiagen) and diluted in microinjection buffer containing 10 mM Tris-HCl (pH 7.8 ...
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bioRxiv - Developmental Biology 2024Quote: ... Cells were transfected using Effectene (Qiagen) in 12-well plates ...
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bioRxiv - Cancer Biology 2024Quote: Viably frozen single-cell suspensions of primary KS skin biopsy samples were thawed and DNA was extracted from isolated cells using DNeasy kit (Qiagen). Then ...
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bioRxiv - Cancer Biology 2024Quote: ... DNA was prepared from a bacterial extract using the EndoFree Plasmid Maxi Kit (#12362, Qiagen) and digested with MluI to release the plasmid backbone ...
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bioRxiv - Immunology 2024Quote: ... Total RNA was the purified from cells by using RNeasy Micro Kit (Qiagen) and checked for purity and integrity in a Bioanalyzer-2100 (Agilent Technologies ...
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bioRxiv - Evolutionary Biology 2024Quote: ... Genomic DNA was extracted from 22 ethanol-preserved specimens using the Qiagen DNeasy Blood & Tissue Kit (Qiagen, Hilden, Germany), and from four formalin-fixed specimens using the QIAamp DNA FFPE Tissue Kit (Qiagen ...
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bioRxiv - Evolutionary Biology 2024Quote: ... We used 100 mg of each dried sample for DNA extraction following the Qiagen DNeasy Plant Mini Kit protocol (Qiagen, Hilden, Germany). The extractions’ quality and concentration were evaluated using GelRed 1.5% agarose gels and a NanoDrop spectrophotometer (Thermo Fisher Scientific ...
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bioRxiv - Evolutionary Biology 2024Quote: ... RNA was extracted from homogenized tissues using a RNeasy column purification kit (Qiagen). We assessed concentration and purity of extracted RNA using a NanoDrop 2000 spectrophotometer (ThermoFisher Scientific) ...
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bioRxiv - Evolutionary Biology 2024Quote: ... and from four formalin-fixed specimens using the QIAamp DNA FFPE Tissue Kit (Qiagen, Hilden, Germany) following the manufacturer’s instructions ...
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bioRxiv - Evolutionary Biology 2024Quote: ... We extracted RNA from these tissues using a guanidium thiocyanate and phenol-chloroform protocol combined with an RNeasy column purification kit (Qiagen) based on the keystone dental gene protocol (43) ...
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bioRxiv - Evolutionary Biology 2024Quote: ... We collected three biological replicates of first molars from both species at three postnatal stages (P1, P15, and P21) and immediately preserved them at −80°C in lysis buffer (Buffer RLT; Qiagen) supplemented with 40 μM dithiothreitol ...
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Insulin Resistance Increases TNBC Aggressiveness and Brain Metastasis via Adipocyte-derived ExosomesbioRxiv - Cancer Biology 2024Quote: ... cDNA sample was mixed with RT2 SYBR Green ROX qPCR Mastermix (Qiagen, 330522) and PCR reactions were performed and results were analyzed using a 7500 Fast Real-Time PCR instrument ...
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bioRxiv - Microbiology 2024Quote: ... Viral DNA was purified and extracted from 100 μL of DNase I-treated culture supernatant using a QIAamp DNA Blood Mini Kit (QIAGEN GmbH, Hilden, Germany). To quantify viral DNA copies ...
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bioRxiv - Microbiology 2024Quote: cDNA was generated from 1 µg of RNA using qScript cDNA SuperMix (QuantaBio) and the cDNA was purified using a QIAquick PCR Purification Kit (Qiagen). 2 µL of cDNA was used in a PCR reaction with primers specific to EF_B0058 and EF_B0059 (Table S2) ...
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bioRxiv - Microbiology 2024Quote: ... PCR product was purified with QIAquick Gel Extraction Kit (Qiagen, Germany) following the manufacturer’s instructions ...
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bioRxiv - Microbiology 2024Quote: ... The resulting reads were mapped to the phi47 genome (NCBI accession ON086985.1) and SNPs were identified using CLC Genomics Workbench v20 (Qiagen) with a minimum coverage of 15 ...
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bioRxiv - Physiology 2024Quote: ... DNase digest was performed on column using the RNase-Free DNase Set (Qiagen 79254). RNA concentration and purity were assessed by UV spectroscopy at ODs of 260 and 280 nm using a Nanodrop (ThermoFisher One/One UV spectrophotometer ...
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Insulin Resistance Increases TNBC Aggressiveness and Brain Metastasis via Adipocyte-derived ExosomesbioRxiv - Cancer Biology 2024Quote: ... RNA was then used to prepare cDNA using QuantiTect Reverse Transcription Kit (Qiagen, 205313). Mouse RT2 Profiler™ PCR Array ...
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Insulin Resistance Increases TNBC Aggressiveness and Brain Metastasis via Adipocyte-derived ExosomesbioRxiv - Cancer Biology 2024Quote: Total RNA was isolated from cells using RNeasy Plus Mini Kit (74136, Qiagen). RNA was then used to prepare cDNA using QuantiTect Reverse Transcription Kit (Qiagen ...
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Insulin Resistance Increases TNBC Aggressiveness and Brain Metastasis via Adipocyte-derived ExosomesbioRxiv - Cancer Biology 2024Quote: ... were purchased from Qiagen. cDNA sample was mixed with RT2 SYBR Green ROX qPCR Mastermix (Qiagen ...
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bioRxiv - Immunology 2024Quote: RNA isolation was performed with the RNeasy+ isolation kit (Qiagen) according to the manufacturer’s instructions ...
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bioRxiv - Genomics 2024Quote: ... and a Rotor-Gene Q (Qiagen) RT-qPCR machine for cycling ...