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Citations for Qiagen :
3401 - 3450 of 10000+ citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genetics 2024Quote: ... Total RNA was further purified using an RNeasy mini RNA isolation kit (Qiagen, Valencia, CA, USA). Column DNase digestion at 25°C for 15 min removed residual DNA ...
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bioRxiv - Genetics 2024Quote: DNA was isolated from frozen retinal tissue with the QIAamp DNA mini kit (QIAGEN, Aarhus, Denmark) following the standard protocol ...
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bioRxiv - Genetics 2024Quote: ... using DNeasy Blood & Tissue Kits (catalog no. 69506; QIAGEN, Valencia, CA). Approximately 1.5 µg of DNA per sample was shipped to Neogen (Lincoln ...
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bioRxiv - Genetics 2024Quote: Total RNA from post-mortem adult human neural retina was extracted following manufacturer’s guidelines (RNeasy Mini kit®, Qiagen) followed by DNase treatment (ArcticZymes ...
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bioRxiv - Genetics 2024Quote: ... A RNeasy Mini Kit (Qiagen #74104) was used for RNA purification ...
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bioRxiv - Immunology 2024Quote: ... and a stainless-steel bead added to allow for tissue disruption and homogenization in a tissue lyser (Qiagen). RNA extraction was completed as instructed by manufacture (Invitrogen ...
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bioRxiv - Immunology 2024Quote: ... The His-tagged IA2 was then purified with a NiNTA Agarose bead (Qiagen, Cat#1018244) packed column fraction collection ...
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bioRxiv - Immunology 2024Quote: ... to be washed and perform a DNAse digest step (Qiagen). RNA was eluted in RNAse-free water ...
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bioRxiv - Genetics 2024Quote: ... All plasmids were purified from bacterial culture using QIAprep Spin Miniprep Kit (Qiagen) and all PCR products were generated with Phusion® High-Fidelity DNA Polymerase (New England BioLabs ...
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bioRxiv - Genetics 2024Quote: ... purified using the RNeasy Mini Kit (Qiagen) and RNA integrity was determined using TapeStation (Agilent Technologies 2200) ...
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bioRxiv - Genetics 2024Quote: ... This was followed by cell lysis in smartseq2 lysis buffer or RLT plus buffer (QIAGEN), respectively.
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bioRxiv - Genetics 2024Quote: ... Total RNA was extracted from PAXgene tubes using whole blood gene RNA purification kits (QIAGEN). RNA from all samples was run on an Agilent Bioanalyzer to assess RNA quality and estimate RNA concentrations ...
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bioRxiv - Genetics 2024Quote: ... The eluate was separated from the beads and RNA was purified using RNeasy Plus Mini Kit (QIAGEN) according to manufacturer’s protocol.
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bioRxiv - Immunology 2024Quote: ... Total RNA was isolated from larvae using the Qiagen miRNeasy mini kit according to the manufacturer’s instructions (Qiagen, Venlo, Netherlands). Quality and integrity of the RNA was checked on an Agilent Bioanalyzer 2100 total RNA Nano series II chip (Agilent ...
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bioRxiv - Immunology 2024Quote: ... RNA was isolated using an RNeasy Plus Mini kit (Qiagen, 74134). RNA was sequenced by the Genome Technology Access Center at the McDonnell Genome Institute at Washington University School of Medicine on an Illumina NovaSeq-6000 to generate 150 bp paired end reads ...
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bioRxiv - Genetics 2024Quote: ... and purified using an RNeasy Mini Kit (Qiagen, CA, United States). The quality of RNA was assessed using an Agilent 2100 Bioanalyzer (Agilent ...
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bioRxiv - Immunology 2024Quote: mRNA was isolated from dura meninges by using RNeasy Mini Kit (Qiagen) following the manufactureŕs protocol ...
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bioRxiv - Immunology 2024Quote: ... The collected media was spun down and passed over a Ni-NTA column (Qiagen) before being stored in 5 mM 3- (N-morpholino ...
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bioRxiv - Genomics 2024Quote: ... Total RNA was extracted with the RNeasy Mini Kit (QIAGEN Cat#74104) according to manufacturer’s instructions and quantified with NanoDrop spectrophotometer ...
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bioRxiv - Genomics 2024Quote: Leaf samples were collected from a single plant of each cultivar and subjected to high-molecular-weight genomic DNA extraction using a Genomic-tip 500/G (Qiagen, Hilden, Germany). Short-read sequence data were obtained to estimate the genome sizes of the Helaly and Fahl cultivars ...
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bioRxiv - Genomics 2024Quote: RNA sequencing—Total RNA from G9 and F3 was extracted using the RNeasy Plant Mini Kit (Qiagen, Hilden, Germany). Each strain had four short-read libraries prepared using the TruSeq Stranded mRNA Library Prep Kit (Illumina ...
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bioRxiv - Genomics 2024Quote: ... A-addition and adapter ligation using the Gene Read DNA Library I Core Kit (Qiagen) according to the manufacturer’s protocol ...
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bioRxiv - Genomics 2024Quote: ... qPCR was performed as above except that RNA was extracted using the RNeasy mini kit (Qiagen). cDNA was then created using the High Capacity cDNA Reverse Transcription Kit (Applied Biosciences ...
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bioRxiv - Genomics 2024Quote: ... genomic DNA was extracted from bulk-transduced cells that did not undergo HCR using the Puregene Cell Kit (Qiagen). Meanwhile ...
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bioRxiv - Genomics 2024Quote: ... PCR products were pyrosequenced with the Pyromark Q48 system (QIAGEN), according to the manufacturer’s protocol and analyzed with PyroMark Q48 Autoprep (QIAGEN).
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bioRxiv - Genetics 2024Quote: ... Briefly approximately 20 mg of liver were mechanically disrupted using a TissueLyser (Qiagen, Manchester, UK) in 600 RLT plus buffer containing β-mercaptoethanol ...
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bioRxiv - Genomics 2024Quote: ... Primers used for the PCR were designed with PyroMark Assay Design 2.0 software (QIAGEN) and listed in Table 3 ...
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bioRxiv - Genetics 2024Quote: ... DNA was then extracted using AllPrep DNA/RNA Mini kit (Qiagen, Manchester UK). Briefly approximately 20 mg of liver were mechanically disrupted using a TissueLyser (Qiagen ...
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bioRxiv - Genetics 2024Quote: ... DNA was then extracted using AllPrep DNA/RNA Mini kit (Qiagen, Manchester UK). Briefly approximately 20 mg of liver were mechanically disrupted using a TissueLyser (Qiagen ...
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bioRxiv - Genetics 2024Quote: ... After PCR clean-up (Qiagen), the UTR library amplicons were appended sequentially with EGFP CDS and CMV promoter sequences (derived from EGFP-N1 vector ...
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bioRxiv - Genetics 2024Quote: ... PCR products were purified with QIAquick PCR Purification Kit (QIAGEN) according to the manufacturer’s protocol ...
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bioRxiv - Genetics 2024Quote: ... PCR products were run in a 2% agarose gel and the correct band (around 200 bp) was purified with QiaQuick gel extraction Kit (QIAGEN, catalog number 28706) (at least 2 columns ...
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bioRxiv - Genetics 2024Quote: ... Briefly approximately 20 mg of liver were mechanically disrupted using a TissueLyser (Qiagen, Manchester, UK) in 600 RLT plus buffer containing β-mercaptoethanol ...
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bioRxiv - Genetics 2024Quote: ... we generated 13 pools of 10 FGOs per genotype in a single well of 8-well strips containing 2.5μl of Buffer RLT (QIAGEN; 79216). Samples were stored in a -80C freezer ...
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bioRxiv - Genetics 2024Quote: ... and eluted in 15μl Buffer EB (QIAGEN). 1ng of pre-amplified DNA was used for tagmentation reaction (7min @55°C ...
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bioRxiv - Genetics 2024Quote: ... Genomic DNA was purified using Quick-DNA microprep plus kit according to the manufacturer’s instructions (Qiagen). Further processing steps through high-throughput sequencing were performed at the University of Michigan Advanced Genomics Core ...
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bioRxiv - Genetics 2024Quote: ... DNA was extracted using the QIAamp DNA mini kit (Qiagen) following the manufacturer’s protocol with the addition of 1mM EDTA added to buffer ATL ...
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bioRxiv - Genetics 2024Quote: ... The digest was incubated for 2 hours at 37°C then purified using a QiaQuick PCR purification kit (Qiagen Cat#28106). Fragments of 2,100 bp were size-selected using a SageELF instrument (Sage Science ...
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bioRxiv - Genetics 2024Quote: ... DNA was extracted from surviving cells (Lucigen QuickExtract or Qiagen DNeasy DNA blood and tissue kit) for characterization and quantification of CRISPR-Cas9-induced mutations ...
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bioRxiv - Genetics 2024Quote: Genomic DNA was extracted with Qiagen DNeasy 96 tissue kits (Qiagen, Valencia, California). TaqMan-PCR using Fluidigm 96.96 Dynamic Arrays (Fluidigm ...
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bioRxiv - Genetics 2024Quote: ... followed by a Qiagen MAXI plasmid prep (Qiagen Cat#12963).
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bioRxiv - Genetics 2024Quote: ... bisulfite-convered genomic DNA was amplified with the PyroMark PCR kit (35 cycles; Qiagen) with primers containing handle sequences ...
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bioRxiv - Genetics 2024Quote: ... PCR products were purified using a MinElute PCR purification kit (Qiagen Cat#28006) using 11 µL water for the final elution ...
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bioRxiv - Genetics 2024Quote: Genomic DNA was isolated from tissues using the DNeasy DNA blood and tissue kit (Qiagen). Somatic CAG instability analysis was performed using a human-specific PCR assay that amplifies the HTT CAG repeat from the knock-in allele (Mouro Pinto et al. ...
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bioRxiv - Genetics 2024Quote: ... 10μl of PCR-product and 2µl of sequencing primer (4μM) were then pyrosequenced on a Pyromark Q48 Autoprep (Qiagen), as described before 22 ...
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bioRxiv - Genetics 2024Quote: ... and PCR amplified at the age-associated regions with the PyroMark PCR kit (Qiagen). 10μl of PCR-product and 2µl of sequencing primer (4μM ...
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bioRxiv - Genetics 2024Quote: ... RNA extraction was performed using AllPrep DNA/RNA Micro Kit (QIAGEN). RNA eluate concentration was quantified using the QUBIT HS RNA kit (Thermo Fisher ...
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bioRxiv - Developmental Biology 2024Quote: ... The resultant libraries were purified again using the MinElute PCR Purification Kit (Qiagen), multiplexed ...
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bioRxiv - Developmental Biology 2024Quote: Genomic DNA was extracted from tissues indicated (30 mg) using the DNeasy Blood & Tissue Kit (Qiagen), according to the manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2024Quote: ... RNA was extracted from each pooled sample using RNeasy® QIAGEN Mini Kit (74104, QIAGEN Inc.) according to manufacture instructions and RNA concentration and quality was confirmed using a NanoDrop UV-Vis Spectrophotometer (Thermo Fisher Scientific ...