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Citations for Qiagen :
301 - 350 of 1224 citations for Mouse Anti Human Papilloma virus types 16 18 716 F10 since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2021Quote: ... Total Nucleic Acid (TNA) was extracted from using automated nucleic acid extraction on the QIAsymphony and the DSP Virus/Pathogen Mini Kit (Qiagen). Autopsy tissues were collected from lung ...
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bioRxiv - Microbiology 2019Quote: For viral genome copy measurements RNA was extracted from 2 µl sucrose purified virus using the RNeasy mini kit (QIAgen). The RNA was then treated with TURBO DNase (Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2022Quote: ... Viral RNA was extracted from supernatants of hamster nasal wash samples using the QIAsymphony DSP Virus/Pathogen Mini Kit on the QIAsymphony instrument (Qiagen). Real time RT-qPCR was then performed using the AgPath RT-PCR (Life Technologies ...
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bioRxiv - Microbiology 2022Quote: Viral RNA extraction was performed on an EZ1 Advanced XL device using the EZ1 virus mini kit V2.0 according to the manufacturer’s recommendations (Qiagen, Courtaboeuf, France). The qPCR was run on a Lightcycler® 480 thermocycler (Roche diagnostics ...
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bioRxiv - Microbiology 2022Quote: ... Viral RNA was extracted from cell culture supernatants or hamster nasal washes using the QIAsymphony DSP Virus/Pathogen Mini Kit on the QIAsymphony instrument (Qiagen). RT-qPCR was then performed using the AgPath RT-PCR (Life Technologies ...
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bioRxiv - Molecular Biology 2020Quote: Total viral RNA was extracted from deactivated samples using automated nucleic acid extraction on the QIAsymphony and the DSP Virus/Pathogen Mini Kit (QIAGEN). One step reverse transcription to cDNA and real-time PCR (RT-PCR ...
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bioRxiv - Microbiology 2020Quote: ... MB897-1) were used to generate infectious virus by transfecting 293T cells using the Effectene transfection reagent (QIAGEN, Valencia, CA). Supernatant containing virus was collected 2 days post transfection ...
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bioRxiv - Microbiology 2020Quote: ... viral RNA was extracted from either DF-1 cultures infected with the WT virus or DF-1P cells using the Qiamp viral RNA mini kit (Qiagen) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2020Quote: ... EAV genome copy number was determined by one step RT-qPCR using the QuantiTect™ Virus + ROX Vial Kit (Qiagen). Each RNA sample was tested in triplicate ...
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bioRxiv - Physiology 2021Quote: ... We infected Sf9 cells with the recombinant virus to express and purify the His-tagged proteins using a nickel-nitrilotriacetic acid (Ni-NTA, Qiagen) column ...
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bioRxiv - Evolutionary Biology 2020Quote: ... RNA from the original material used for next-generation sequencing of the serum and passage 3 cultured MRI103 virus was used as the template for amplification using the One-Step Ahead RT-PCR kit (Qiagen).
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bioRxiv - Microbiology 2020Quote: ... virus pellets were resuspended by vortexing and community DNA was extracted using the QIAamp Viral RNA Mini Kit (Qiagen, Germany) following the manufacturer’s protocol ...
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bioRxiv - Immunology 2020Quote: ... RT-qPCR was performed with a primer set targeting partial regions of the ORF1b gene in the SARS-CoV-2 virus using the QIAGEN OneStep RT-PCR kit (Qiagen) as previously reported (51) ...
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bioRxiv - Genomics 2021Quote: ... Total Nucleic Acid (TNA) was extracted using automated nucleic acid extraction on the QIAsymphony and the DSP Virus/Pathogen Mini Kit (Qiagen). RNA isolation and library preparation is fully described in Butler ...
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bioRxiv - Immunology 2022Quote: ... we isolated viral RNA directly from the Fluenz Tetra vaccine (AstraZeneca; season 2017/2018) virus using the Qiagen Viral RNA Isolation Kit (Qiagen) following the manufacturer’s instructions ...
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bioRxiv - Synthetic Biology 2022Quote: ... The cells were cultured in growth media containing virus for 60 hours and total RNA was then extracted with the RNeasy kit (Qiagen). From the total RNA ...
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bioRxiv - Microbiology 2023Quote: Total genomic DNA from the bacteriophages and bacterial isolates was extracted using QIAamp UltraSens Virus kit (Qiagen catalog number 53706) and DNeasy Blood & Tissue Kit (Qiagen catalog number 69504 ...
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bioRxiv - Systems Biology 2023Quote: Viral RNA was extracted from specimens using the QIAamp Viral RNA Minikit and the QIAamp 96 Virus QIAcube HT Kit (Qiagen). Viral transport media (VTM)-only controls were included in each extraction ...
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bioRxiv - Microbiology 2023Quote: DNase/RNase treated VLPs were used for genomic DNA extraction using Qiagen QIAamp DSP Virus Spin Kit (Qiagen, MD, U.S.A) following the manufacturer’s protocol ...
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bioRxiv - Microbiology 2023Quote: ... round 1 and 3 virus infections of GeCKO-A549 cells using the midi gDNA extraction kit (Qiagen, Germantown, MD, USA). The sgRNA’s DNA copies were PCR amplified from the extracted gDNAs for next generation sequencing (Fig 1) ...
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bioRxiv - Microbiology 2023Quote: ... Nucleic acids were extracted from 140 µl of each sample and also from the original virus dilutions with the “QIamp viral RNA extraction kit” (Qiagen) and eluted in 60 µl elution buffer according to manufacturer’s instructions ...
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bioRxiv - Microbiology 2023Quote: ... A single colony was picked with an inoculation loop and DNA extraction was performed on the QIAsymphony using the DSP Virus/Pathogen Mini Kit (Qiagen). Library preparation performed using Nextera XT (Illumina Inc. ...
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bioRxiv - Microbiology 2024Quote: 200µL of lung of infectious cell supernatant (virus stock and HAE) was inactivated with an equal volume of VXL lysis buffer (Qiagen) and viral RNA was extracted using an EZ1 Advanced XL robot with the EZ1 mini virus 2.0 kit (both from Qiagen ...
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bioRxiv - Microbiology 2024Quote: ... reverse transcription quantitative PCR (RT-qPCR) assays were performed with the QuantiTect Virus + ROX Vial Kit (Qiagen, Toronto, ON, Canada) in a LightCycler® 480 system (Roche Molecular System) ...
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bioRxiv - Microbiology 2022Quote: ... 27620, 1:2,000 dilution) (rabbit anti-Spike S1, Cell Signaling, Cat #: 99423, 1:2,000 dilution) (mouse anti-Strep, Qiagen, Cat #: 34850, 1:2,000 dilution) (rabbit anti-ORF8 ...
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bioRxiv - Genomics 2020Quote: ... kidney, gut, skin and vertebrae) and 18 pcw (skin, kidney and heart) samples were fixed in PAXgene Tissue FIX (Qiagen) for 4 and 24 hours respectively and embedded in paraffin using a Tissue-Tek VIP machine (Sakura) ...
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bioRxiv - Microbiology 2021Quote: ... The remaining 18 μl of RT-PCR DNA was purified by a QIAquick PCR Purification kit (Qiagen, Germantown, MD, USA). Sequences of the purified RT-PCR products were generated using a BigDye Terminator v3.1 cycle sequencing kit (Applied Biosystems ...
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bioRxiv - Biochemistry 2020Quote: Initial crystals of Nup133NTD-VHH-SAN5 were obtained at 18°C in 1 day as part of the Protein Complex suite (Qiagen) in a 96-well sitting drop tray with a reservoir containing 15% PEG MME 2,000 ...
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bioRxiv - Microbiology 2020Quote: ... DNA from site AC at 18 cm depth was extracted by the Loy lab using the DNeasy PowerSoil Kit (Qiagen), following manufacturer’s instructions ...
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bioRxiv - Microbiology 2021Quote: DNA was extracted from 18 cultures that were growing in LB+colistin (2mg/L) using DNeasy Blood and Tissue Kit (QIAGEN)
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bioRxiv - Neuroscience 2021Quote: ... and rplp0 (all QuantiTect Primer Assays: 18s Cat. No. QT02448082, gapdh Cat. No. QT01658692, hprt1 Cat. No. QT00166768, rplp0 Cat. No. QT00249375; Qiagen). We analyzed the relative expression of the following genes of interest ...
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bioRxiv - Microbiology 2021Quote: ... (1 μl) for 18 h and the RNA was extracted by using the mirCury cell and plant isolation kit (QIAGEN). A final number of 108 treated cells were lysed using 500 μL of glass beads and the 600 μL of the Lysis buffer provided with the kit ...
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bioRxiv - Microbiology 2020Quote: ... iniae QMA0248 grown for 18 h on Todd-Hewitt agar from a master seed stock (non-subcultured) with the Genomic Tip 20 kit (Qiagen). Pre-incubation for 2 h at 37°C of cells suspended in 500 μL 50 mM EDTA containing 200 units of mutanolysin and 2 mg lysozyme was found to improve cell lysis prior to following the manufacturers protocol for purification of high molecular weight DNA ...
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bioRxiv - Microbiology 2020Quote: ... and 18 centimeters below seafloor (cmbsf) from station AC of Van Keulenfjorden (Supp. Table 1) using the DNeasy PowerSoil Kit (Qiagen) according to the manufacturer’s protocol ...
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bioRxiv - Microbiology 2022Quote: ... A total of 1,046,709,676 reads from 18 samples were generated and the fastq files were imported into CLC Genomics Workbench version 21.0.5 (QIAGEN Bioinformatics, Inc.). The reads were aligned to Homo sapiens hg38 reference genome (downloaded from Ensembl via the CLC Genomics Workbench ...
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bioRxiv - Neuroscience 2023Quote: ... Total RNA of pools of larval eyes (18-28 per sample) was extracted using the RNeasy Plus Mini kit (Qiagen). RNA was reverse transcribed to cDNA using the Super Script III First-strand synthesis system (Invitrogen ...
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bioRxiv - Evolutionary Biology 2023Quote: ... The supernatant was then purified using a modified PB buffer and eluted using 2 washes in 18 μl buffer EB (QIAGEN) - with 3 min of incubation time at 37°C (Dabney et al ...
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bioRxiv - Microbiology 2023Quote: Genomic DNA was extracted as previously described with standard methods (18) using the DNeasy blood and tissue extraction kit (Qiagen) and eluted in 200 ul of MilliQ water ...
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bioRxiv - Biochemistry 2023Quote: ... Lysates were clarified by centrifugation at 5 °C at 18 500 rpm for 30 min and loaded to gravity flow Ni-NTA agarose resin (QIAGEN) previously equilibrated with wash buffer WB2_1 (50 mM Tris-HCl pH 8 ...
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bioRxiv - Microbiology 2021Quote: ... putida type strain cells (200 μl) was extracted using MagAttract HMW DNA Kit (Qiagen). The DNA was eluted in 100 uL AE buffer ...
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bioRxiv - Genetics 2023Quote: ... Multiplex reactions were carried out with the Type-it Microsatellite PCR Kit (Qiagen, Germany). The final volume of the PCR reactions was optimised to 6 μl ...
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bioRxiv - Neuroscience 2024Quote: Total RNA from all cell types was isolated using the RNeasy Mini Kit (Qiagen) following manufacturer’s instructions ...
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bioRxiv - Cell Biology 2023Quote: ... siRNA for ANXA2 (Human, Qiagen, Hs_ANXA2_8, SI02632385) or siRNA for Anxa2 (Mouse ...
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bioRxiv - Biochemistry 2020Quote: ... The primary antibodies were used at the following dilutions: 1:1000 anti-penta-His mouse monoclonal (Qiagen), 1:5000 anti-cMyc mouse monoclonal (Sigma) ...
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bioRxiv - Microbiology 2021Quote: ... stored at −20° C and characterized by immnoblotting using mouse anti-2xStrep tag (Qiagen #34850, 1:1,000), and secondary goat anti-mouse IgG IRDye® 800CW (LI-COR # 926-32210 ...
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bioRxiv - Biochemistry 2022Quote: ... Anti-His Western blots were performed using Penta-His mouse monoclonal IgG1 as primary antibody (Qiagen, #34660) at 1:2000 dilution ...
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bioRxiv - Molecular Biology 2022Quote: ... Protein bands were transferred to a nitrocellulose membrane and probed with primary antibodies [mouse anti-His (Qiagen) to detect CAT ...
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bioRxiv - Cancer Biology 2021Quote: ... The expression of the BCL-2 anti-apoptotic genes in the NPC cell lines were accessed using the custom RT2 Human Apoptosis Profiler PCR array (Qiagen, Hilden, Germany). To delineate the contribution of MCL-1 for cell survival ...
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bioRxiv - Microbiology 2021Quote: ... To generate a recombinant A_NS237 virus, viral RNA was extracted from allantoic fluid using Trizol reagent (Thermo Fischer, Germany) and Qiagen RNeasy Kit (Qiagen, Germany) following the manufacturers’ guidelines ...
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bioRxiv - Genomics 2020Quote: ... Viral RNA was extracted from 300 µl of viral transport media (VTM, Himedia, India) using QIAamp 96 Virus QIAcube HT Kit (Qiagen, Germany), following the manufacturer’s instructions ...