Labshake search
Citations for Qiagen :
451 - 500 of 1224 citations for Mouse Anti Human Papilloma virus types 16 18 716 F10 since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Genomics 2022Quote: ... The mixture was incubated at 16°C for 2 h and the resulting double-stranded DNA was purified using the MinElute PCR Purification kit (Qiagen) with 20μl EB for elution ...
-
bioRxiv - Microbiology 2023Quote: ... Cells were sorted into 96-well plates containing lysis buffer with proteinase K solution in PKD buffer (1:16) (Qiagen) and were stored at -80ºC ...
-
bioRxiv - Microbiology 2023Quote: ... and Chl523R (5’ CCY YMC GTA TTA CCG CAG CT 3’) targeting the 16S rRNA gene on a QIAcuity One digital PCR device (Qiagen) as recommended by the manufacturer ...
-
bioRxiv - Cancer Biology 2023Quote: RNA from 100 µL of serum (35 µL for samples from pregnant dams and 15 µL for samples from P14-16 pups) or 200 µl conditioned media was isolated using the miRNeasy Serum/Plasma Advanced Kit (Qiagen) according to the manufacturer’s instructions ...
-
bioRxiv - Bioengineering 2023Quote: ... Total RNA was isolated from iPSCs and organoids on days 16 and 31 by using the RNeasy Plus Mini Kit (74134, Qiagen) with the manufacturer’s recommended protocol ...
-
bioRxiv - Microbiology 2023Quote: ... The polyA selection step was replaced by an rRNA depletion step with the QIAseq FastSelect - 5S/16S/23S bacterial rRNA removal kit (Qiagen). Libraries were quantified by a fluorometric method (QubIT ...
-
bioRxiv - Developmental Biology 2024Quote: ... thresholds were used to prepare Reduced Representation Bisulfite Sequencing (RRBS) libraries using the Ovation RRBS Methyl-Seq System 1-16 (NuGen) and the EpiTect Fast DNA Bisulfite kit (Qiagen). Libraries were sequenced using the NextSeq500 platform (Illumina ...
-
bioRxiv - Genetics 2024Quote: ... PCR was performed with 2 µL (∼16 ng) or 4 µL (∼32 ng) of BT-converted DNA with HotStar Taq Polymerase (Qiagen), depending on the genomic region ...
-
bioRxiv - Cell Biology 2019Quote: ... Human primers were pre-validated Quantitect primers (Qiagen, Manchester, UK). Comparative quantification normalised target gene mRNA to β-actin (ACTB ...
-
bioRxiv - Microbiology 2022Quote: ... DNA from human samples was extracted with PowerSoil Pro (Qiagen) on the QiaCube HT (Qiagen) ...
-
bioRxiv - Microbiology 2021Quote: ... the human Stress & Toxicity PathwayFinder RT2 Profiler™ technology (Qiagen), assessing expression of 84 stress response-related genes ...
-
bioRxiv - Cancer Biology 2022Quote: ... individual Human SHANK3 siRNA_2 (Cat. no. S100717710 Hs_SHANK3_2 siRNA, Qiagen) and individual ON-TARGETplus Human SHANK3 siRNA_7 (Cat ...
-
bioRxiv - Cancer Biology 2022Quote: ... and Human Serum/Plasma miRCURY LNA miRNA PCR array (Qiagen, Catalog Number ...
-
bioRxiv - Molecular Biology 2023Quote: A siRNA library targeting all human kinases (Qiagen, Hilden, Germany) was utilized ...
-
bioRxiv - Immunology 2023Quote: ... RNA from human PBMCs was isolated using RNeasy Kit (QIAGEN) according to the manufacturer’s instructions ...
-
bioRxiv - Synthetic Biology 2020Quote: ... For SARS-CoV-2 antigen detection the blots were incubated with 1:2000 Anti-His mouse monoclonal primary antibody (Qiagen, #34660) and then incubated with 1:10000 peroxidase labelled anti-mouse IgG secondary antibody (GE Healthcare) ...
-
bioRxiv - Biochemistry 2022Quote: ... Memorial Sloan Kettering Cancer Center) at a dilution of 1:5,000) and His (mouse anti-penta-His antibody (Qiagen, cat. #34660) at a dilution of 1:10,000 ...
-
bioRxiv - Microbiology 2021Quote: ... Nucleic acid was extracted from 200 μl of each sample using the QIAamp MiniElute™ Virus Spin Kit nucleic acid (for DNA and RNA) according to the manufacturer (QIAgen Canada, Toronto, ON, Canada). DNA samples were stored at −80°C and assayed in duplicate by qPCR ...
-
bioRxiv - Genetics 2021Quote: ... The PCR mix (final volume of 15.75 µL) consisted of: 7.5 µL Type-it Multiplex PCR Master Mix (QIAGEN), 3 µL Q solution (5X) ...
-
bioRxiv - Cancer Biology 2019Quote: The cDNA of wild-type ecATCase and regulatory chain of ecATCase-holo were amplified by PCR (Qiagen Kit) using BL21(DE3 ...
-
bioRxiv - Molecular Biology 2022Quote: ... genomic DNA from wild type (WT) and YBX1 KO cells was extracted using DNeasy Blood & Tissue Kit (Qiagen) and amplified using the primers 5’-GGGTAATGAGGCTACAACTGTTT-3’ and 5’-GTCAACTCTAACACATTTCCACGTAT-3’ ...
-
bioRxiv - Microbiology 2021Quote: Total RNA extraction was performed from the wild-type and mutant clones using RNeasy plus mini kit (QIAGEN). RNA was quantified by qubit 4 ...
-
bioRxiv - Biochemistry 2021Quote: Total RNAs were extracted from wild-type or DACR HAP-1 with the RNeasy Plus Mini Kit (Qiagen) according to the manufacturer’s instructions ...
-
bioRxiv - Neuroscience 2022Quote: ... the tissue was homogenised to a fine powder using a ball mill-type grinder (Tissuelyser II Qiagen, 85300). For the homogenization of the frozen material one liquid nitrogen cooled 5 mm stainless steel metal balls was added to each Eppendorf tube and the frozen material was disintegrated for 1 min at 25 Hz.
-
bioRxiv - Genomics 2023Quote: DNA from both types of samples was extracted using a commercially available kit (DNeasy Blood & Tissue Kit, QIAGEN) following the manufacturer’s protocol ...
-
bioRxiv - Molecular Biology 2021Quote: ... 2000 ng of plasmid DNA was transfected into each well using Effectene (16 μL of enhancer and 30 μL of Effectene reagent; Qiagen 301427). As above ...
-
bioRxiv - Microbiology 2021Quote: We extracted the total genomic DNA (DNA) from 16 samples (Data S1) using an automated DNA extraction platform with DNeasy PowerSoil Kit (QIAGEN, Germany) according to manufacturer’s instructions ...
-
bioRxiv - Systems Biology 2022Quote: Extraction of DNA for both 16S rRNA gene sequencing and shotgun metagenomics was performed using a DNeasy UltraClean Microbial Kit (Qiagen, Germany), and the extracted DNA was quantitated with a Qubit fluorometer ...
-
bioRxiv - Genetics 2022Quote: ... and non-inoculated G305-3M leaf segments collected along 10 different time points (0, 3, 6, 9, 12, 16, 24, 36, 48, 72 hpi) using the RNeasy Plant Mini Kit (Qiagen, Germany). The cDNA was synthesized from total RNA using a qScript™ cDNA Synthesis Kit (Quantabio ...
-
bioRxiv - Developmental Biology 2023Quote: Total RNA was isolated from 16 placentas (3-4 placentas/fetal sex/group) using an RNAeasy kit (Qiagen, Venlo, The Netherlands). Isolated RNA was stored at −80 °C in nuclease-free water ...
-
bioRxiv - Genomics 2023Quote: The bacterial 16S rRNA V4 variable region was amplified for 5 cycles with the HotStarTaq Plus Mastermix Kit (Qiagen, Hilden, Germany). Samples were purified with Ampure XP beads (Beckman Coulter ...
-
bioRxiv - Cell Biology 2024Quote: ... into 4 million HEK293-GP cells in 300 µl Buffer EC with 16 µl Enhancer and 60 µl Effectene Transfection Reagent (Qiagen 301425) (Morgenstern and Land ...
-
bioRxiv - Bioengineering 2021Quote: ... Membranes were blocked with 5% milk in PBST (Phosphate buffer saline, 0.05% Tween 20) and incubated with monoclonal mouse anti-His antibody (Penta His, Qiagen, dilution 1:1000) according to the manufacturers’ instructions ...
-
bioRxiv - Cancer Biology 2021Quote: ... RNA of PDOs grown in ‘Type 1’ culture medium was isolated according to the manufacturer’s protocol using the RNeasy Mini Kit (QIAGEN). Quality and quantity of the RNA samples and the libraries were measured with Agilent’s Bioanalyzer2100 and Invitrogen™ Qubit™ 3.0 Fluorometer ...
-
bioRxiv - Immunology 2021Quote: The indicated cell types were harvested and stored at −80°C until RNA was isolated using RNeasy kit (Qiagen) following manufacturer’s instructions ...
-
bioRxiv - Immunology 2019Quote: dram1 or dram1Δ19N (negative control) RNA was isolated from wild type or dram1Δ19n/Δ19n embryos using QIAzol lysis reagent (79306, QIAGEN) and purified with the RNeasy MinElute Cleanup kit (74204 ...
-
bioRxiv - Neuroscience 2020Quote: Total RNA was isolated from wild type and auts2ancb104 heterozygote and homozygote embryos using an RNeasy Mini kit (QIAGEN), and first-strand cDNA was synthesized from 1 ⎧g of total RNA by oligo(dT ...
-
bioRxiv - Genomics 2021Quote: RNA was extracted from validated clones and wild-type HAP1 cells using the RNeasy Mini Kit (Qiagen cat # 74104) following the manufacturer’s instructions and then treated with TURBO DNA-free™ Kit (Life Technologies cat # AM1907 ...
-
bioRxiv - Microbiology 2020Quote: ... cells were transfected with wild-type CrPV or CrPV-DcDV RNA using the TransMessenger transfection reagent (Qiagen, Hilden, Germany) according to the manufacturer’s instructions and the transfected cells were incubated at 26 °C ...
-
bioRxiv - Genetics 2020Quote: ... and cells treated with transfection mix without siRNA (Wild type (WT)) or non-targeting control siRNA (scrambled (SCR)) (Qiagen catalog SI03650318 ...
-
bioRxiv - Evolutionary Biology 2023Quote: ... DNA and RNA were extracted from the B14804-/- and wild-type cells using AllPrep DNA/RNA Mini kit (Qiagen). cDNA was synthesized from extracted RNA using SuperScript™ III Reverse Transcriptase (Invitrogen ...
-
bioRxiv - Molecular Biology 2023Quote: ... rdr1;2;6 Cen5-ATHILA5 ddm1 (hp5) and their corresponding DDM1 wild-type siblings with the DNeasy Plant Pro Kit (Qiagen). From each sample ...
-
bioRxiv - Microbiology 2023Quote: ... oxydans wild-type cultures at OD600 0.3 units (Exp) or 3 units (Sta) using the RNeasy Mini Kit (Qiagen), following the manufacturer’s instructions ...
-
bioRxiv - Neuroscience 2021Quote: ... astrocytes and human brain tissue using the RNeasy Mini kit (Qiagen) and reverse transcribed using the High-Capacity RNA-to-cDNA kit (ThermoFisher Scientific) ...
-
bioRxiv - Cancer Biology 2021Quote: The Human Cancer Stem Cells RT² Profiler PCR Array from Qiagen was used to profile the expression of 84 genes linked to cancer stem cells (CSCs ...
-
bioRxiv - Cancer Biology 2022Quote: Human EMT qPCR arrays were purchased from Qiagen (Cat. #: PAHS-021Z), performed as described using RNA from PDX mammary tumors grown in SOFT and STIFF Col1/rBM hydrogels ...
-
bioRxiv - Physiology 2022Quote: ... Mouse Obesity (PAMM- 017ZC-12) array and Mouse Aging (PAMM-178ZC) from Qiagen (Maryland, USA), according to the manufacturer’s instructions ...
-
bioRxiv - Microbiology 2019Quote: ... the V4 region of the bacterial 16S rRNA was amplified using the Hot Start Taq Plus Master Mix (Qiagen, Germantown, MD, USA) and indexed primers [52] ...
-
bioRxiv - Plant Biology 2021Quote: ... The DNA amplicons derived from the bacterial 16S rRNA gene were extracted from the gels using the QIAquick Gel Extraction Kit (Qiagen, Hilden, Germany). After determination of the DNA concentration of each amplicon (nanodrop ...
-
bioRxiv - Microbiology 2022Quote: ... Dual-index barcodes and adapters were added to amplified products through a second-round of PCR using the QIAseq 16S/ITS 96-Index I array (Qiagen, Hilden, Germany). Cycling conditions were ...