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Citations for Qiagen :
3151 - 3200 of 3437 citations for Fipronil 3 Cyano Pyrazole 3 4 5 13C4 99%; 3 Cyano 5 15N2 98% 100 Ug Ml In Methanol since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2023Quote: ... The aqueous phase was removed and transferred to MaxTract High Density 2 mL tubes (Qiagen Inc, Valencia, CA, USA). Samples were then extracted a second time as described above and the aqueous phase from the repeated extractions for each sample were combined ...
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bioRxiv - Microbiology 2022Quote: ... 1 to 2 mL of culture were mixed with the same volume RNAprotect™ Bacteria Reagent (Qiagen, Hilden, Germany) incubated for 10 min and centrifuged ...
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bioRxiv - Immunology 2022Quote: His-tagged RBD of SARS-CoV S was diluted to a concentration of 0.8 μg/mL in Tris-buffered saline (TBS) and immobilized on NiNTA 96-well plates (Qiagen) for 2h at RT ...
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bioRxiv - Molecular Biology 2022Quote: ... cfDNA was extracted from 0.5 ml of plasma or serum using the QIAmp DNA Mini Blood kit (Qiagen, CA), according to the ‘‘Blood and body fluid protocol’’ and our own detailed protocol [37] ...
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bioRxiv - Microbiology 2022Quote: ... and then homogenized tissues in 1 ml PBS containing a penicillin/streptomycin mixture by using a TissueLyser II (Qiagen) at 30-Hz oscillation frequency for 3 min ...
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bioRxiv - Immunology 2022Quote: ... All samples were homogenized in PBS (1 mL for cecal content and 0.5 mL for fecal pellets and mLNs) for 2.5 min at 25 Hz in a TissueLyser (Qiagen, Germany). In the case of mLNs ...
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bioRxiv - Cancer Biology 2024Quote: ... and genomic DNA was extracted from a 0.5 mL aliquot of whole blood with the DNeasy Blood and Tissue Kit (69506, Qiagen) according the protocal’s instructions ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... The lysate was centrifuged at 50,000×g for 10 min and to the supernatant 1 mL of 50% Ni−NTA (Qiagen) was added and incubated for 1 h at 4 °C ...
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bioRxiv - Cell Biology 2023Quote: Undifferentiated myoblasts (day 0) and differentiated myotubes (day 7) were collected and lysed in 1 mL of Qiazol (Qiagen). RNA from undifferentiated and differentiated myoblasts was extracted using the Qiagen miRNeasy kit (Qiagen ...
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bioRxiv - Evolutionary Biology 2023Quote: ... Genomic DNA was extracted from 2 mL of each of the nine overnight cultures (DNeasy Blood & Tissue Kit; Qiagen). Library preparation and sequencing was performed by Novogene Europe (Cambridge ...
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bioRxiv - Plant Biology 2023Quote: ... Leaf discs were ground in 1 mL sterile water with the help of a tissue lyser (QIAGEN, TissueLyser II) using 1.5 mL safe-lock Eppendorf tubs containing three 2.4 mm metal beads ...
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bioRxiv - Cell Biology 2023Quote: ... Cells were harvested by centrifugation (2500 x g, 3.5 min) and washed with 1 mL of RNAse-free water (Qiagen) before being spun down (10k rpm ...
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bioRxiv - Immunology 2023Quote: ... Both biopsies were homogenized in 350 mL of Buffer RLT and then extracted using the RNeasy Micro kit (Qiagen) with on-column DNase digestion ...
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bioRxiv - Molecular Biology 2023Quote: ... Clarified bacterial lysates from a 1 l culture were bound to a 0.5 ml column of Ni-NTA resin (Qiagen) by gravity flow ...
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bioRxiv - Microbiology 2022Quote: ... 2 mL of logarithmic growth phase culture was pelleted by centrifugation and resuspended by vortexing in B1 buffer (Qiagen, Hilden Germany ...
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bioRxiv - Cell Biology 2023Quote: ... genomic DNA was extracted by incubation with 6 ml of lysis buffer (50 mM Tris pH 8.0, 50 mM EDTA, 1% SDS, 0.1 mg/ml proteinase K (Qiagen, 19131)) at 55°C for 16 hours ...
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bioRxiv - Biochemistry 2023Quote: ... The plasmid was transformed into stbl3 competent E.Coli and DNA was harvested from 200 mL growth media using an endotoxin free plasmid purification kit (Qiagen).
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bioRxiv - Microbiology 2023Quote: The supernatant was collected and at room temperature was passed over a 2.0 mL bed volume of Ni-NTA Sepharose resin (Qiagen). The column was then washed three times with 10.0 mL of wash buffer (50.0 mM Tris-HCl [pH 8.0] ...
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bioRxiv - Neuroscience 2023Quote: ... This was followed by three washes with 2X SSC at 37°C and treatment with RNaseA (200ng/ml; Qiagen) in 2X SSC at 37°C ...
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bioRxiv - Molecular Biology 2023Quote: ... Samples were moved on magnet and supernatant was transferred to clean 1.5 mL tubes for nucleic acid extraction using the MinElute PCR Purification Kit (Qiagen). Purified DNA was used for library preparation using the NEBNext Ultra Library prep Kit (Illumina ...
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bioRxiv - Immunology 2023Quote: ... The supernatant containing target proteins was passed through a polypropylene column containing 2 ml Ni-NTA resin from Qiagen. After the target protein bound to the Ni-NTA resin ...
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bioRxiv - Synthetic Biology 2022Quote: ... 0.1 mg/mL Lysostaphin (MilliporeSigma) was added in the pre-treatment step in combination with enzymatic lysis buffer (Qiagen). Bacterial strains are listed in Table S2.
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bioRxiv - Synthetic Biology 2022Quote: ... 0.1 mg/mL Lysostaphin (MilliporeSigma) was added in the pre-treatment step in combination with enzymatic lysis buffer (Qiagen). Purified DNA was stored at −20°C before further processing for NGS or cell-based detection.
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bioRxiv - Cancer Biology 2023Quote: DNA was extracted from fecal and cecal samples following 40 min of vortexing in 0.5 ml of Qiagen PM1 buffer (Qiagen) with 200 mg of 106/500 μm glass beads (Sigma ...
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bioRxiv - Immunology 2023Quote: ... The protein complexes were adjusted to 8.6–12 mg/ml and screened for crystallization using the 384 conditions of the JCSG Core Suite (Qiagen) on our robotic CrystalMation system (Rigaku ...
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bioRxiv - Biochemistry 2023Quote: ... Lysates were clarified by centrifugation at 24,000 g for 30 min and applied to a 2 mL column bed of Ni-NTA resin (Qiagen) for purification by IMAC ...
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bioRxiv - Evolutionary Biology 2023Quote: ... and its genomic DNA was amplified in the same 0.2-mL PCR tube using the REPLI-g single-cell WGA kit (QIAGEN) to obtain single-worm WGS data from LJ2050 ...
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bioRxiv - Developmental Biology 2023Quote: Blastocysts were washed with DPBS containing 1 mg/ml polyvinylpyrrolidone (PBS-PVP) and transferred into 50 μl droplets of 0.1% protease (Qiagen) to remove the zona pellucida ...
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bioRxiv - Bioengineering 2023Quote: ... Deparaffinization with Xylene and RNA extraction was performed in sterile 1.5 ml microcentrifuge tubes with the RNeasy FFPE Kit (Qiagen), according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2023Quote: ... The tissue pellets were resuspended in 1 ml of FSW using a tissue lyser (Tissue-Lyser II, Qiagen, Australia) and the resuspension was homogenized using sterile glass homogenizers for 30 s ...
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bioRxiv - Immunology 2023Quote: The post-caval lobe of the lung was weighed and homogenized in 1 mL of PBS (pH 7.4) using a TissueLyser LT (Qiagen). RNA was extracted using TRIzol™ LS Reagent (Invitrogen ...
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bioRxiv - Neuroscience 2023Quote: ... The resulting total cell lysate was clarified by centrifugation (30,000 g for 25 min) and the supernatant fraction was applied to a 4.5 mL packed Ni-NTA resin (Qiagen) and gently rocked at 4 °C for 1 h in 50 mL conical tubes ...
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bioRxiv - Genomics 2023Quote: ... Plasmid transfections were performed in six-well tissue-culture treated dishes at 1.8 × 10^6 cells/ml using Effectene (301427; QIAGEN) following the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2023Quote: ... Ribosome pellets were resuspended in 0.7 ml Qiazol and mRNA was recovered using the miRNeasy RNA extraction kit (Qiagen) according to the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2024Quote: ... The culture was then combined with LB + 25 µg/mL chloramphenicol + 0.2% glucose (w/v) culture for overnight growth (37 °C) and then miniprepped (Qiagen).
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bioRxiv - Microbiology 2024Quote: ... beads were resuspended in 0.2 mL of 1xPBS supplemented with Proteinase K from the DNeasy Blood and Tissue Kit (QIAGEN) and incubated at 37 °C in a thermomixer for 4 h ...
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bioRxiv - Biochemistry 2024Quote: ... High titre baculovirus was produced by transfecting bacmid DNA into Sf21 cells at 0.5 × 106 cells/ ml cells using Superfect (Qiagen) in 24 deep-well blocks ...
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bioRxiv - Molecular Biology 2024Quote: ... The lysate was centrifuged at 44,800 g for 45 min and the supernatant was incubated with 2.5 ml Ni-NTA agarose beads (Qiagen,30210) for 1 h at 4°C ...
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bioRxiv - Microbiology 2020Quote: ... protein were adjusted to around 11 mg/mL and screened for crystallization using the 384 conditions of the JCSG Core Suite (Qiagen) on our custom-designed robotic CrystalMation system (Rigaku ...
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bioRxiv - Microbiology 2020Quote: ... We immediately transferred all swabs and film to a microcentrifuge tube with 1 mL lysis buffer and garnet homogenization beads (Qiagen NV ...
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bioRxiv - Biochemistry 2021Quote: ... Cells with lysis buffer were pulverized in a 10 mL jar containing a 12 mm grinding ball using a TissueLyser II (QIAGEN) by performing 5 rounds of 3 min at 15 Hz ...
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bioRxiv - Genetics 2020Quote: ... Ligation was carried out overnight at 16°C followed by overnight cross-link removal with 20mg/ml Proteinase K (Qiagen). The samples were purified using phenol-chloroform and ethanol precipitated resulting in 3C libraries ...
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bioRxiv - Microbiology 2020Quote: ... We sampled 1 ml of the clear phase for living planktonic cells and extracted DNA using DNeasy Blood & Tissue Kit (Qiagen). The cultures were also plated on SHIEH-agar plates with dilutions for cfu/ml -estimation and colony-PCR.
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bioRxiv - Microbiology 2020Quote: ... Each complex was adjusted to 12 mg/ml and screened for crystallization using the 384 conditions of the JCSG Core Suite (Qiagen) on our custom-designed robotic CrystalMation system (Rigaku ...
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bioRxiv - Molecular Biology 2022Quote: ... A serial dilution series of E.coli ranging from 107 to 100 CFU/ml was established and 200µl of each dilution was extracted using the MagnaExtract method compared to the DNeasy™ (QIAGEN). Each extraction was performed in triplicate and underwent high resolution melt (HRM ...
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bioRxiv - Genomics 2020Quote: ... at which point we combined the resulting solution with 12 mL of PB buffer and followed standard MinElute PCR Purification Kit (Qiagen) protocol ...
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bioRxiv - Genomics 2019Quote: ... with the addition of 20 μl of proteinase K (20 mg/ml) followed by incubation at 56°C for 1-2 hr and the mitochondrial DNA was extracted by Qiagen DNeasy Blood & Tissue Kit (QIAGEN Inc.) ...
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bioRxiv - Molecular Biology 2019Quote: ... Pellet was treated with 20 μg lysostaphin (1 mg/ml) and RNA isolation was performed using the RNeasy Plus mini kit (QIAGEN) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2020Quote: ... Kinetochore proteins were conjugated to these beads using antibodies that recognize specific proteins or their tags: biotinylated anti-His-tag antibodies (6 µg ml−1, Qiagen), biotinylated anti-GFP antibodies (20 µg ml−1 ...
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bioRxiv - Microbiology 2019Quote: Genomic DNA was extracted from 2 mL of overnight growth in BHI broth using the DNeasy Blood and Tissue kit (Qiagen). Genome sequencing of the two isolates was performed on a Miseq platform using Illumina technology ...