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Citations for Qiagen :
3051 - 3100 of 3437 citations for Fipronil 3 Cyano Pyrazole 3 4 5 13C4 99%; 3 Cyano 5 15N2 98% 100 Ug Ml In Methanol since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genetics 2020Quote: ... plasmids were isolated from 200 mL of these cultures using Qiagen Plasmid Plus Purification kits (Qiagen #12963, #12965). The remaining 200 mL of the overnight cultures were spun down ...
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bioRxiv - Systems Biology 2020Quote: Three milliliters of cells from mid-log phase cultures were mixed with 6 mL RNAprotect Bacteria Reagent (Qiagen). Samples were mixed immediately by vortexing for 5 s ...
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bioRxiv - Biochemistry 2020Quote: ... the supernatant was applied to a 0.1 ml column of Ni2+-NTA Superflow resin (Qiagen Cat No. 30410) and protein was purified using the manufacturer’s protocol ...
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The formation of a fuzzy complex in the negative arm regulates the robustness of the circadian clockbioRxiv - Molecular Biology 2022Quote: ... The soluble fraction was split between two columns each with 2 ml Ni-NTA agarose beads (Qiagen, 30210) pre-equilibrated with Lysis Buffer ...
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bioRxiv - Developmental Biology 2022Quote: ... The concentrated SPACA6-containing media was applied onto a 2-mL column of Ni-NTA agarose resin (Qiagen). The Ni-NTA resin was washed with 10 column volumes (CV ...
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bioRxiv - Immunology 2022Quote: ... The upper aqueous phase containing RNA was carefully transferred to a 2 ml collection tube (cat. 990381, Qiagen) without touching the interphase and placed in a QIAcube instrument for extraction.RNA extraction was carried out using the recommended protocol (FIW-50-001-J_FW_MB and PLC program version FIW-50-002-G_PLC_MB ...
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bioRxiv - Microbiology 2021Quote: ... the cell lysate was loaded by gravity flow onto a column containing 1 ml Ni-NTA resin (Qiagen) equilibrated with buffer A ...
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bioRxiv - Developmental Biology 2022Quote: ... SPACA6 concentrated to 7 mg mL−1 in Buffer B was mixed in a 1:1 volumetric ratio (0.3:0.3 mL) with JCSG+ (Qiagen), Cryos (Qiagen) ...
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bioRxiv - Cell Biology 2022Quote: ... The clarified lysate was supplemented with Ni-NTA agarose (1.3 ml resin/ 1 l insect cell pellet, Qiagen) and incubated for 30 mins at 4 °C on a rotating wheel ...
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bioRxiv - Plant Biology 2020Quote: ... The clear supernatant was then gently mixed with 1 ml of Ni+2-NTA agarose beads (Qiagen, Germany) for 30 min and then added to the column with a flow rate of 0.5 ml/min ...
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bioRxiv - Biochemistry 2019Quote: ... At the indicated time point the samples were added immediately to 2 mL of RNAprotect Bacterial Reagent (QIAGEN), immediately vortexed ...
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bioRxiv - Molecular Biology 2020Quote: ... 1.5 mL of overnight culture was spun down and resuspended in 250 µL P1 buffer with RNAse (Qiagen) and 200 µL of glass beads (Sigma ...
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bioRxiv - Microbiology 2020Quote: ... We then washed beads four times in 1 ml of Modified RLT buffer (RLT buffer supplied by Qiagen with added 10 mM Tris pH 7.5 ...
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bioRxiv - Genomics 2021Quote: ... cfDNA was isolated from 2 ml of plasma using either the manual QIAamp circulating nucleic acid kit (Qiagen), or the semi-automated QIAsymphony DSP Circulating DNA Kit (Qiagen ...
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bioRxiv - Microbiology 2021Quote: ... PBS/G was replaced with PBS/G containing 0.2 mg/mL proteinase K (Qiagen N. V., Hilden, Germany) or various concentrations of sodium azide ...
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bioRxiv - Microbiology 2021Quote: Mouse lung tissues were homogenized in 1 ml of MEM-free media using TissueRuptor (Qiagen, Chadstone, Victoria, Australia). The homogenate was obtained by centrifugation at 6,000 × g for 5 min at 4℃ ...
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bioRxiv - Genetics 2019Quote: ... the filter was compressed inside of the microcentrifuge tube using a pipette tip and the supernatant was transferred to a clean 0.5mL (NaOH extraction) or 1.5 mL microcentrifuge tube (magnetic beads and QIagen).
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bioRxiv - Molecular Biology 2022Quote: ... cfDNA was extracted from 0.5 ml of plasma/serum using the QIAmp DNA Mini Blood kit (Qiagen, CA), performed according to the ‘‘Blood and body fluid protocol’’ and our own detailed protocol [37] ...
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bioRxiv - Microbiology 2022Quote: ... 20 μL of EDTA (final concentration 20 mM) and 1.3 μL of Proteinase K (20 mg/mL) (Qiagen) were added and incubated at 56 °C for 1.5 h ...
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bioRxiv - Microbiology 2022Quote: ... The purified DNA pellets were resuspended in 60 µL Tris-HCl pH 7.5 with 0.8 mg/mL RNAse A (Qiagen) and incubated at 37°C for 30 min ...
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bioRxiv - Microbiology 2022Quote: ... Total RNA was isolated from 1 ml of cells from each culture using a miRNeasy kit (Qiagen, Germany) with some modifications ...
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bioRxiv - Molecular Biology 2022Quote: ... coli grown for 16 h in 250 ml of 2×YT medium using the Plasmid Maxi Kit (QIAGEN) in accordance with the manufacturer’s protocol ...
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bioRxiv - Neuroscience 2023Quote: ... Tissue was placed into 2-mL round-bottom homogenizer tubes pre-loaded with Stainless Steel beads (#69989; Qiagen) and filled up with lysis buffer (#AA-LYS-16ml ...
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bioRxiv - Neuroscience 2022Quote: ... qPCR was conducted using the following 10x QuantiTect primers (diluted in 1.1 mL TE pH 8.0, final concentration: 200nM) purchased from Qiagen: OTR (QT100379036 ...
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bioRxiv - Plant Biology 2022Quote: Root and shoot tissues were disrupted in 1.5 mL Eppendorf tubes using a tissue homogenizer (Qiagen TissueLyser II) with 3.2 mm chrome steel beads (BioSpec Products) ...
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bioRxiv - Systems Biology 2022Quote: ... Aliquots of 2 mL of cell cultures were submitted to DNA extraction using DNeasy Blood & Tissue kit (QIAGEN). DNA samples were quality checked and genotyped using PCR to confirm strains (Table S5) ...
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bioRxiv - Molecular Biology 2024Quote: ... ∼80 uL packed irradiated embryos and control embryos were resuspended in 2 mL buffer G2 (Qiagen, catalog # 1014636) and digested with proteinase K (1 mg/mL ...
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bioRxiv - Biochemistry 2023Quote: ... The supernatant was loaded using a peristaltic pump in 1 mL (bed volume) of Ni-NTA agarose (QIAGEN) packed in a Poly-Prep® Chromatography Column (Cat #731-1550 ...
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bioRxiv - Biochemistry 2024Quote: ... ∼8 ml PCR product (azide-DNA:2xbiotin-DNA = 1:1) was purified using HiSpeed Plasmid Maxi kit (Qiagen) in 1 ml NaHCO3 (pH 8.3) ...
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bioRxiv - Microbiology 2022Quote: ... bacterial cells reaching an OD620nm = 0.6 were pelleted (5000 x g, 15 min) and immediately fixed in 1.5 ml RNA-protect (Qiagen) for 10 min before being processed using a PureLink RNA mini kit (Ambion ...
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bioRxiv - Genomics 2023Quote: ... The mixture was then transferred to a 2 mL MaXtract High Density tube (Cat#129056, Qiagen, Hilden, Germany) for phase separation ...
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bioRxiv - Biochemistry 2023Quote: ... Clarified lysate was then filtered through a 0.45 µm syringe filter and mixed with 1.5 mL of washed Ni-NTA Agarose beads (Qiagen). The mixture was allowed to bind under nutation at 4℃ for 2 hr ...
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bioRxiv - Biochemistry 2023Quote: ... and grown in LB medium (ampicillin 50 μg mL−1) and plasmid purified (miniprep kit Qiagen, Hilden, Germany). Mutations were confirmed by DNA sequencing (Eurofins Genomics ...
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bioRxiv - Microbiology 2023Quote: ... and up to 6 ml of culture per isolate per medium was harvested using Bacterial RNAprotect (Qiagen 76506). Three biological replicates were generated for each condition.
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bioRxiv - Molecular Biology 2023Quote: ... Plasmid DNA was purified from 130 mL of liquid culture using a HiSpeed Plasmid Maxi Kit (Qiagen 12663).
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bioRxiv - Cancer Biology 2023Quote: ... Total cell-free DNA from urine supernatants was extracted from a median of 3.6 mL urine supernatant (range 0.7–3.65 mL) incubated with 10% ATL buffer before purification using the QIAsymphony DSP Circulating DNA Kit (Qiagen).
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bioRxiv - Microbiology 2023Quote: ... Cells were then disrupted by sonication and the supernatant was mixed with 1 mL Ni-NTA agarose (QIAGEN) and incubated for 1 h at 4 °C with gentle shaking ...
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bioRxiv - Microbiology 2023Quote: ... 1 mL of culture was withdrawn and chromosomal DNA extracted using the Dneasy blood and tissue kit (Qiagen). The samples were normalized according to DNA concentrations and diluted 1:5 ...
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bioRxiv - Microbiology 2023Quote: ... The filters were stored in 1.5-mL cryotubes previously filled with 600 µL of buffer RLT Plus (Qiagen), 6 µL of 2-mercaptoethanol (Sigma-Aldrich) ...
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bioRxiv - Neuroscience 2023Quote: ... qPCR was conducted using the following 10x QuantiTect primers (diluted in 1.1 mL TE pH 8.0, final concentration: 200nM) purchased from Qiagen: OTR (QT100379036 ...
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bioRxiv - Evolutionary Biology 2023Quote: ... Genomic DNA was isolated from 1 mL of overnight culture of each genotype (DNeasy Blood & Tissue Kit; Qiagen). Paired-end ...
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bioRxiv - Microbiology 2024Quote: ... and freshwater samples (50 ml filtered onto sterile 0.2µm filter papers) was extracted using the DNeasy PowerSoil Kit (Qiagen), according to manufacturer’s instructions ...
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bioRxiv - Immunology 2024Quote: ... Tissues were then resuspended in 1 mL of lysis buffer from the RNeasy Fibrous Tissue Mini Kit (Qiagen) and processed as per the manufacturer’s protocol ...
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bioRxiv - Microbiology 2024Quote: ... dialized proteins were supplemented with 10 mM Imidazole and ran over 7.5-mL equilibrated Ni-NTA beads (Qiagen). The flowthrough was collected and concentrated using 30 kDa molecular weight cut-off centrifugal filters (Merck Millipore ...
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bioRxiv - Microbiology 2024Quote: ... dialized proteins were supplemented with 10 mM Imidazole and ran over 7.5-mL equilibrated Ni-NTA beads (Qiagen). The flowthrough was collected and and concentrated using 100 kDa molecular weight cut-off centrifugal filters (Merck Millipore ...
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bioRxiv - Molecular Biology 2024Quote: ... followed by a 15-minute incubation at room temperature with 1.25 ml Qiazol lysis reagent (Qiagen, cat# 79306). All samples were stored at 80°C until further processing.
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bioRxiv - Biophysics 2021Quote: cfDNA was isolated from 2 mL of plasma for each sample via the QIAamp Circulating Nucleic Acid kit (Qiagen). ddPCR was performed using the QX200 Droplet Digital PCR System (Bio-Rad) ...
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bioRxiv - Cell Biology 2020Quote: ... Cells were washed once with 1 % BSA and incubated with a solution containing 200 μg/ml RNase A (Qiagen) and 50 μg/ml Propodium Iodide (Sigma ...
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bioRxiv - Cancer Biology 2022Quote: ... tumors were chopped into small pieces using a razor blade and incubated with 1.8 mL tissue lysis buffer ATL (Qiagen) and 0.2 mL Proteinase K (Qiagen ...
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bioRxiv - Microbiology 2022Quote: ... Pellets were then resuspended in 500 μL of supernatant and stabilized with 1 mL of RNAprotect Bacteria Reagent (Qiagen). The RNeasy® Protect Bacteria Mini Kit (Qiagen ...