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Citations for Qiagen :
251 - 300 of 2406 citations for Marker 7 Pcb Mixture W Pcb 118 13C12 99% 100 Ng Ml In Nonane since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2021Quote: ... we pre-coated 384-well plates with a mixture containing the PCR primer mix (Qiagen; sequences and category numbers available through online data repository (17)) ...
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bioRxiv - Developmental Biology 2022Quote: ... The cell-TRIzol mixtures were transferred to collection tubes and homogenized with Tissuelyser II (Qiagen). Chloroform was added in a 1:5 ratio by volume and phase separation was performed ...
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bioRxiv - Plant Biology 2024Quote: ... The mixture was used as input for RNA cleanup using the RNeasy kit (Qiagen #74104) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2023Quote: ... the mixture was incubated with Glutathione Sepharose™ 4B (Cytiva) and Ni-NTA Superflow (Qiagen). The Hs53BP1 fragment without its GST tag was recovered in the flow through and concentrated using Vivaspin concentrator (Sartorius).
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bioRxiv - Cancer Biology 2023Quote: ... The precipitated mixture was transferred to a DNeasy Mini spin 96-well spin plate (Qiagen), washed twice with 700 μl buffer AW1 and twice with buffer AW2 ...
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bioRxiv - Cell Biology 2019Quote: ... 7 days-old seedlings and flower buds with the RNeasy Plant Mini kit (Qiagen) following manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2021Quote: ... total RNA was collected from 7-12hr embryos per manufacturer’s specification (RNeasy kit, Qiagen), and submitted to Novogene (Sacramento ...
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bioRxiv - Bioengineering 2019Quote: ... Cells were harvested after 3 or 7 days post-transfection (RNEasy mini kit, Qiagen), whereby cDNA was made and amplified via qPCR with primers for GAPDH ...
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bioRxiv - Immunology 2019Quote: ... Multi-species alignment and cladrograms were constructed using CLC Main Workbench 7 (CLCbio, Qiagen).
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bioRxiv - Microbiology 2020Quote: ... were homogenized 7 min at 50 pulses s-1 with the Tissuelyser LT (QIAGEN) prior to incubation in lysis buffer for 1 h at 60°C for increasing cell lysis ...
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bioRxiv - Neuroscience 2021Quote: ... 1mM DTT and 100 µg/ml cycloheximide) and eluted in 350 µl of RLT Plus buffer from the RNAeasy Plus Micro kit (Qiagen) supplemented with 2-mercaptoethanol (Bio-Rad).
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bioRxiv - Microbiology 2021Quote: ... The incubation period at 56°C was set to one hour for all samples and all samples were treated with 4 µl RNase A (100 mg/ml, Qiagen). DNA was eluted in 100 µl TE buffer with 0.1 mM EDTA ...
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bioRxiv - Cancer Biology 2022Quote: ... Library plasmid pools were propagated in liquid culture in LB with ampicillin (100 μg/ml) at 30 °C overnight and extracted (Qiagen).
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bioRxiv - Microbiology 2019Quote: U1 cells were treated with 100 μg/ml concentration of exosomes for 12 h and total RNA was isolated using RNeasy mini kit (Qiagen) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2021Quote: The frozen tumors were minced to small pieces and suspended in buffer P1 (QIAGEN, 1 mL Buffer/100 mg tumor) supplemented with 100 μg/mL RNase A (Promega) ...
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bioRxiv - Immunology 2021Quote: ... ~100 mg tissue was homogenized in 1 mL Qiazol and RNA was extracted using RNeasy Lipid Tissue Mini Kit (Qiagen) according to the manufacturer’s instructions ...
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bioRxiv - Genetics 2022Quote: Total RNA was extracted from nearly 100 µl of tightly packed age-synchronized adult worm pellet collected in 1 ml TRIzol reagent provided by Qiagen RNeasy Mini kit (Cat ...
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bioRxiv - Neuroscience 2023Quote: ... 1mM DTT and 100 µg/ml cycloheximide) and eluted in 350 µl of RLT Plus buffer from the RNAeasy Plus Micro kit (Qiagen) supplemented with 2-mercaptoethanol (Bio-Rad).
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bioRxiv - Microbiology 2023Quote: ... Then 4 μl RNase A (100 mg/ml) were added to the tubes followed by 150 μl of buffer AL (lysis buffer, Qiagen). After mixing by vortexing ...
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bioRxiv - Biophysics 2023Quote: ... We grew sequence-positive clones for the plasmid extraction at 30°C overnight in presence of a selective antibiotic Ampicillin (100 µg/ml) and isolated the final plasmid via a Midiprep using a Qiafilater plasmid midi kit (Qiagen) the following day.
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bioRxiv - Immunology 2023Quote: ... Plasmid DNA from overnight liquid cultures (LB with 100 mg/ mL ampicillin) was isolated with QIAprep Spin Miniprep Kit (Qiagen).
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bioRxiv - Synthetic Biology 2023Quote: ... The organs were weighed and lysed in 1 mL Triton X-100 solution (0.1% in PBS) using a TissueLyser II (QIAGEN, Germany) according to the manufacturer protocol ...
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bioRxiv - Genomics 2023Quote: ... After ligation nuclei were pelleted and resuspended in cold PBS with DAPI to a final concentration of 300nM and GFP+ cells were FAC-sorted into a 96 well plate with 2ul lysis buffer (20mM Tris pH 8, 20mM NaCl, 0.15% Triton X-100, 25mM DTT, 1mM EDTA, 500nM Carrier ssDNA, and 15ug/mL Qiagen Protease) and lysed for 1 hour at 50°C and inactivated 15 minutes at 70°C ...
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bioRxiv - Genomics 2023Quote: ... then stopped with transposome removal buffer (300nM NaCl, 45 mM EDTA, 0.01% Triton X-100 with 100ug/mL Qiagen Protease) and incubated at 50°C for 40 minutes and 70°C for 20 minutes ...
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bioRxiv - Neuroscience 2024Quote: ... and 20 U/mL RNasin) and resuspended in 100 µL of RLT buffer from the RNeasy Micro Kit (Qiagen 74004) supplemented with 1% β-mercaptoethanol ...
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bioRxiv - Neuroscience 2024Quote: ... 1mM DTT and 100 µg/ml cycloheximide) and eluted in 350 µl of RLT Plus buffer (RNAeasy Plus Micro kit, Qiagen) supplemented with 2-mercaptoethanol (Bio-Rad).
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bioRxiv - Immunology 2020Quote: RNA was isolated from FACS sorted mouse B1a and B2 cells based on markers listed above with QIAamp RNA Blood mini kit according to manufacturer’s instructions (Qiagen, Venlo, The Netherlands). RNA was eluted with 30μL H2O ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: The gene network analysis for the selected 10 marker genes was conducted using Ingenuity Pathway Analysis Software (Version 01-22-01; Ingenuity Systems, Qiagen, CA, USA). This analysis aligned the marker genes with established genetic pathways and networks ...
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bioRxiv - Physiology 2019Quote: ... The LVis function of the FLUOstar Omega microplate reader was used to measure RNA concentrations to ensure all samples for each participant had the same amount of RNA (184 ng - 400 ng) and samples were reverse transcribed to cDNA using the RT2 First Strand kit (Qiagen, UK, 330401). Quantitative RT-PCR analysis was performed using custom designed 384-well RT2 PCR Profiler Arrays (Qiagen ...
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bioRxiv - Evolutionary Biology 2020Quote: ... A fixed amount of RNA (300 ng/sample = 50 ng/μl) was then reverse transcribed into cDNA using the QuantiTect Reverse Transcriptase kit (QIAGEN, Venlo, Netherlands).
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bioRxiv - Genomics 2020Quote: ... The nuclei were embedded in 0.5 % w/v agarose followed by treatment with proteinase-K (Qiagen) for 2 h ...
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bioRxiv - Immunology 2021Quote: ... Lungs and brain were homogenised in PBS (10% w/v) using a rotor-star homogeniser (Qiagen) and total RNA extracted using TRIzol (Invitrogen ...
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bioRxiv - Immunology 2020Quote: ... A minimum of 60 ng bisulfite-treated (EpiTect; Qiagen) genomic DNA was used for qRT-PCR to quantify ∑ TSDR demethylation ...
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bioRxiv - Plant Biology 2022Quote: ... Forty ng of the amplified and column-purified (Qiagen) DNA was cloned into the universal domestication plasmid pUPD2 by restriction ligation reaction with BsmBI and T4 ligase (both Thermo Scientific) ...
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bioRxiv - Neuroscience 2023Quote: ... 200 ng RNA was hybridized to FastSelect probes (QIAGEN) for ribodepletion ...
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bioRxiv - Molecular Biology 2020Quote: ... The DNA mixture was subsequently purified using a DNeasy Blood and Tissue Kit (Qiagen, Hilden, Germany) following the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2019Quote: ... Cells were trypsinized and 0.5 × 106 cells were plated directly with Effectene transfection mixture (Qiagen, 301427) on 0.1% gelatin (in-house production ...
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bioRxiv - Microbiology 2021Quote: ... The resulting digestion mixture was processed using a DNeasy Blood and Tissue Kit (Qiagen, Hilden, Germany) following manufacturer instructions ...
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bioRxiv - Cancer Biology 2020Quote: ... extracted cell clusters were incubated with a cell lysis mixture (20µL RLT Plus buffer (Qiagen 1053393), plus 1µL of spike-in RNA (1:250,000) ...
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bioRxiv - Molecular Biology 2020Quote: ... Tissues were homogenized in a mixture of chloroform and methanol (2:1) using TissueLyser II (Qiagen) and dried in a Vacufuge (Eppendorf ...
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bioRxiv - Microbiology 2021Quote: ... The PCR mixture contained 1x master mix for the Type-it Microsatellite PCR Kit (Qiagen, France), 3 µL of Q-Solution and 0.25 µM of each primer in a total volume of 30 µL ...
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bioRxiv - Pathology 2020Quote: ... 500μl of liver/Trizol mixture was used for chloroform extraction using MaXtract™ (Qiagen, cat# 129056) and RNeasy® Mini Kit (Qiagen ...
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bioRxiv - Immunology 2020Quote: ... Total RNA was isolated from Trizol/cell lysate mixture by using RNAeasy Mini Kit (Qiagen #74104). Total RNA was sequenced on Illumina NextSeq using Illumina TruSeq Stranded Total RNA library Prep and paired-end sequencing ...
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bioRxiv - Neuroscience 2022Quote: ... The ethanol-containing mixture was spun in two rounds through the RNeasy Mini Spin column (Qiagen) at 10,000rpm for 1 minute each ...
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bioRxiv - Microbiology 2023Quote: ... The mixture was then transferred to DNeasy Mini Spin column (Qiagen Pty. Ltd, Australia, Cat. #69504), and DNA was extracted following the manufacturer’s guidelines.
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bioRxiv - Microbiology 2023Quote: ... Each reaction contained 5 μL DNA solution and PCR mixture with Taq Type-it (Qiagen®). PCR products were analyzed by capillarity electrophoresis on an ABl3130xl sequencer ...
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bioRxiv - Molecular Biology 2023Quote: ... 5 μL of each reaction mixture was used for amplification with the REPLI-g kit (Qiagen) at 16 °C overnight and cleaned with the DNA Clean & Concentrator kit to yield samples for sequencing ...
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bioRxiv - Microbiology 2024Quote: ... The mixture was placed on a Qiagen RNeasy mini column (RNeasy Mini Kit, Qiagen, Hilden, Germany) and centrifuged at 8,000 × g for 15 s to bind RNA into the column ...
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bioRxiv - Genetics 2021Quote: ... Electroporated bacteria was split across six tubes and each recovered in 2 mL of SOC for 1 h at 37 °C then added to 500 mL of LB with 100 μg/mL of carbenicillin and grown for 9 h at 37 °C prior to plasmid purification (Qiagen, 12991). The plasmid prep was then normalized to 1 μg/μL to generate the final mpra:minP:gfp library used for transfection delivery.
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bioRxiv - Evolutionary Biology 2020Quote: ... Approximately half the volume of each feces sample (100-200 mg) was immersed in 3 ml of Inhibit EX Buffer (QIAGEN, Hilden) and stored at room temperature ...