Labshake search
Citations for Qiagen :
101 - 150 of 2406 citations for Marker 7 Pcb Mixture W Pcb 118 13C12 99% 100 Ng Ml In Nonane since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
Cell-free DNA as a biomarker for prostate cancer: elevated concentration and decreased fragment sizebioRxiv - Cancer Biology 2020Quote: ... DNA was extracted from 7 to 55 mL of plasma using the Qiagen QIAamp Circulating Nucleic Acid Kit (Qiagen, Redwood City, CA), and double eluted with 40 μL of Qiagen Elution Buffer ...
-
bioRxiv - Pathology 2021Quote: ... Let-7 miRNA primers were purchased from QIAGEN.
-
FGF signalling is involved in cumulus migration in the common house spider Parasteatoda tepidariorumbioRxiv - Developmental Biology 2021Quote: CLC Main Workbench 7 (QIAGEN Aarhus A/S) was used to perform a local tBLASTn [25] against the Parasteatoda tepidariorum official AUGUSTUS gene set (https://i5k.nal.usda.gov/Parasteatoda_tepidariorum ...
-
bioRxiv - Physiology 2021Quote: Approximately 100 ng/µL of total DNA was extracted from 20 mg tissue (QiAMP DNA mini kit Qiagen, Valencia, CA, USA). DNA from bone marrow was extracted from the bone marrow suspension and centrifuged for 10 min at 2000 g ...
-
bioRxiv - Immunology 2019Quote: Total RNA yields from the PBMC and stimulated PBMCs were in the range of 3 micrograms (50-100 ng/μl in 30μl Qiagen EB buffer) determined by absorbance at 260nm on the NanoDropTM One (Thermo Fisher Scientific) ...
-
bioRxiv - Neuroscience 2023Quote: ... samples obtained by FACS together with 100 ng carrier RNA (PolyA- and PolyA+ mixed in 98:2 ratio) were lysed by Buffer RLT Plus (Qiagen, USA). Then we used PolyA mRNA Magnetic Isolation Module (NEBNext ...
-
bioRxiv - Developmental Biology 2023Quote: ... Bisulfite conversion was performed on 100 ng of genomic DNA using the EpiTect Fast DNA Bisulfite kit from Qiagen (Cat.# 59824) and DNA libraries were constructed using the NUGEN Ovation RRBS Methyl-seq System (Cat.# 0353 ...
-
bioRxiv - Cell Biology 2023Quote: ... all samples were diluted to the same RNA concentration and 250 ng of RNA from each sample spiked with 100 ng mouse RNA to control reaction efficiency were reverse transcribed using the miScript II RT kit (Qiagen, 218161) with HiFlex Buffer according to manufacturer’s instructions ...
-
bioRxiv - Cancer Biology 2023Quote: MiR libraries were generated from 100 -150 ng of cell using the QIAseq miRNA Library Kit according to the protocol (Qiagen, Germany). Library quality control and concentrations were determined using a High Sensitivity D1000 ScreenTape with TapeStation Analysis Software A.02.02 (Agilent Technologies ...
-
bioRxiv - Developmental Biology 2021Quote: ... Lysates were treated with 4 μl of 100 mg/ml RNase A (QIAGEN) at 37 °C shaking at 500 RPM for 3 hours ...
-
bioRxiv - Genomics 2020Quote: ... 1 ul (1U/ul) UDG and 1 ul RNaseA (100 mg/ml, Qiagen) and reactions were incubated at 37°C for 3 hrs ...
-
bioRxiv - Cell Biology 2020Quote: ... Cells were then incubated with 100 μg/ml RNase A solution (Qiagen, 19101). 50 mg/liter Propidium Iodide solution (Sigma-Aldrich ...
-
bioRxiv - Developmental Biology 2020Quote: ... The following day samples were treated with 100 μg/mL RNAse A (Qiagen) for 1 hr at 37°C ...
-
bioRxiv - Molecular Biology 2023Quote: ... 0.5% Triton X-100) with 50 µl protinase K (>600 mAU/ml, Qiagen) and incubated at 37°C for 30min ...
-
bioRxiv - Pharmacology and Toxicology 2019Quote: ... Plasmids were transformed into JM109 chemically competent cells and grown overnight in 250 mL of LB broth with 100 µg/mL of ampicillin and purified using the HiSpeed Plasmid Maxi Kit (Qiagen).
-
bioRxiv - Synthetic Biology 2019Quote: ... grown over night in 5 mL LB and 100 μg mL-1 ampicillin and DNA were purified using a QIAprep Spin Miniprep Kit (QIAGEN). Sequencing was performed by GATC using the primers in SI Appendix Table S4 ...
-
bioRxiv - Genomics 2019Quote: ... and GYG1 (ID# s6360) together with a fluorescent marker (Allstars Neg.siRNA AF488, Qiagen, Cat# 1027292) using DharmaFECT 1 (GE Healthcare ...
-
bioRxiv - Genetics 2019Quote: ... genomic DNA was extracted from a saturated culture of 100 ml under anaerobic condition (YPD) using the genomic tip-100 kit (Qiagen, Courtaboeuf, FRANCE). Paired-end Illumina sequencing libraries were prepared from sonicated genomic DNA according to manufacturer protocols (Genomic DNA Sample Preparation ...
-
bioRxiv - Microbiology 2021Quote: ... weighed (w/v) and homogenized using TissueLyzer® (Qiagen, Germany). The total RNA was extracted from homogenized tissues using Trizol and RNeasy Kit following the manufacturer’s instructions (Qiagen ...
-
bioRxiv - Microbiology 2022Quote: ... 25% (w/v) PEG 1500 (QIAGEN PACT screen, condition 42).
-
bioRxiv - Plant Biology 2020Quote: ... 100-150 ng/μL of DNA was extracted from 100 mg of ground leaf tissue using DNeasy Plant Mini Kit (Qiagen Inc., Canada) following the manufacturer’s protocol ...
-
bioRxiv - Neuroscience 2022Quote: cDNA was prepared using 100 ng RNA from FANS-purified GFP+ OPC nuclei or from optic tracts (RNeasy Mini kit; Qiagen Cat# 74104) and 1.5 μL of 100 ng/L of random hexamers (Integrated DNA Technologies Cat# 51-01-18-26 ...
-
bioRxiv - Evolutionary Biology 2021Quote: ... refolded protein mixture was loaded on Ni-NTA beads (Qiagen) as 1ml per 2 liter culture ...
-
bioRxiv - Biochemistry 2020Quote: ... the reaction mixtures were purified using MiniElute purification kits (Qiagen) and subjected to A-tailing reactions ...
-
bioRxiv - Plant Biology 2021Quote: ... The mixture was shaken using a tissuelyzer (Tissuelyzer II, Qiagen) for 5 minutes at 30 hz and subsequently sonicated for 5 minutes ...
-
bioRxiv - Genomics 2019Quote: ... PCR reaction mixtures contained 10x Standard Taq Reaction buffer (Qiagen), 2 mM MgCl2 ...
-
bioRxiv - Genomics 2020Quote: ... The mixture was transferred to MaXtract High Density tubes (Qiagen) and centrifuged for 8 minutes at 15,000 rpm ...
-
bioRxiv - Microbiology 2019Quote: ... a mixture of the transfection reagent HiPerFect (Qiagen, Hilden, Germany) and serum-free cell culture medium was applied and this was incubated for 5-10 min at room temperature (RT) ...
-
bioRxiv - Molecular Biology 2020Quote: ... the mixture was transferred to a QIAshredder (QIAGEN, Cat#79656) and the AllPrep DNA/RNA Mini Kit (Qiagen ...
-
bioRxiv - Genetics 2021Quote: ... Genomic DNA was extracted from the red blood cells of the parents and 99 F2 hybrid females using the DNeasy Blood & Tissue Kit (Qiagen). The library was constructed according to a previously described method [27] ...
-
bioRxiv - Microbiology 2019Quote: A total of 250 ng of Qst was incubated with 1 ml Ni-NTA Superflow beads (Qiagen, Hilden, DE) in an end-over-end shaker for 1 h at 4°C ...
-
bioRxiv - Cancer Biology 2022Quote: ... 15 μL of lysis buffer (Triton X-100 0.4%, Qiagen Protease 0.1 AU/mL) were added to each well and samples were incubated at 56 °C for 10 minutes and 72 °C for 20 minutes ...
-
bioRxiv - Developmental Biology 2019Quote: ... 0.5% (v/v) Triton X-100) and 1 mg/ml Proteinase K (QIAGEN, Germany). The solution was incubated at 55°C for 15 min ...
-
bioRxiv - Systems Biology 2024Quote: ... Following a 2-hour RNase A treatment (Qiagen, 100 mg/ml, 1:1,000 dilution) at 37°C ...
-
bioRxiv - Cell Biology 2021Quote: ... All were designed using CLC Genomics Workbench 7 (Qiagen) and synthesized by Eurofins Genomics (http://www.eurofins.com) ...
-
bioRxiv - Evolutionary Biology 2021Quote: ... 7 µL of PolyFect transfection reagent (QIAGEN, Duesseldorf, Germany) was added ...
-
bioRxiv - Genomics 2022Quote: ... equal volumes of the clones were combined together and the pool expanded in 20 mL of LB with 100 μg/mL carbenicillin for 9 hr followed by plasmid purification (Qiagen, 12943). Approximately 250 colonies in total were harvested.
-
bioRxiv - Microbiology 2020Quote: ... and then with (2) 15 µl Proteinase K [>600 mA/ml] and 5 µl Rnase A [100 mg/ml] (Qiagen, Germany) for 5min at room temperature ...
-
bioRxiv - Systems Biology 2023Quote: ... Spheroblasts were collected at RT and 2,500 g for 5 min and then re-suspended in 1.6 mL per 100 mL culture homemade buffer P1 (according to the manufacturers protocol, QIAGEN, Hilden, Germany). 1.6 mL per 100 mL culture of homemade buffer P2 (according to the manufacturers protocol ...
-
bioRxiv - Microbiology 2019Quote: ... The PCR mixture contained 0.2 μM of each primer and 40 ng of bacterial DNA in a total volume of 100 μL (HotStar Taq Master Mix Kit, Qiagen, Valencia, CA, USA) (60).
-
bioRxiv - Plant Biology 2022Quote: ... DNA was resuspended in 30 μL of mQ water and incubated with 1 μL of RNase (10 mg/mL prepared from a stock of 100 mg/mL of RNase A from Qiagen, Darmstadt, Germany) at 37 °C for 1 h ...
-
bioRxiv - Genomics 2020Quote: ... Cells were seeded in 6-well plates at a density of 1×106 cells/mL and transfected with 200 ng of plasmid using the Effectene transfection kit (Qiagen). Cells were harvested following 3 days of incubation.
-
bioRxiv - Molecular Biology 2023Quote: cDNA was produced from 1 μg of total RNA (final cDNA concentration 5 ng/ml) using QuantiTech Reverse Transcription kit (Qiagen) according to the manufacturer’s instructions.
-
bioRxiv - Microbiology 2022Quote: ... The mixture was then added to a RNeasy MinElute column (Qiagen) and RNA was reisolated according to manufacturer instructions.
-
bioRxiv - Microbiology 2019Quote: ... Take 100µl of this mixture and use DNA isolation kit (Qiagen) for DNA isolation according to the manufacturer’s instruction.
-
bioRxiv - Immunology 2019Quote: ... and the mixture was added into customized RT2RNA PCR Array (Qiagen, Hilden ...
-
bioRxiv - Genetics 2021Quote: ... The mixture was added to an RNeasy Column (Qiagen Cat # 74101) and processed according to manufacturer’s protocol including the on-column DNase I treatment (Qiagen Cat # 79254).
-
bioRxiv - Bioengineering 2021Quote: ... and the mixture spun on an RNA-cleanup column (Qiagen; miRNeasy). This RNA was then recovered and re-electroporated into cells at the reduced quantity of 7.5 µg ...
-
bioRxiv - Cell Biology 2021Quote: ... The reaction mixture was purified using a PCR purification kit (Qiagen) and eluted in RNase-free water ...
-
bioRxiv - Genomics 2021Quote: ... the mixture was purified with Qiagen MiniElute Purification kit (QIAGEN, 28004) and eluted in 20 µl of elution buffer ...