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Citations for Qiagen :
251 - 300 of 303 citations for CdSeTe ZnS Quantum Dots NIR region Water solvent 760nm since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genomics 2023Quote: ... Isolated colonies were suspended in PCR-grade water for the following automated DNA extraction using QIAcube and QIAmp mini kit (QIAGEN). The DNA extracts were used for SBT ...
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bioRxiv - Neuroscience 2022Quote: ... The RNA pellet was resuspended in RNAase-free water and any DNA digested using the RNase-Free DNase kit (Qiagen). RNA was eluted using the RNeasy MinElute Cleanup Kit (Qiagen) ...
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bioRxiv - Immunology 2022Quote: ... Samples were centrifuged once more and RNA pellets were air-dried for 30-60 minutes at room temperature before resuspending in 30 μl RNase-free water (Qiagen). The quality and quantity of RNA was measured using a NanoDrop Microvolume spectrophotometer ...
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bioRxiv - Evolutionary Biology 2023Quote: ... resuspended in 50 ml of distilled water and the protocol was modified to include 0.1 mm glass beads (Qiagen, Germany) to increase DNA extraction by mechanical lysis ...
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bioRxiv - Immunology 2024Quote: ... cDNA was diluted to 5 ng/µl in RNase-free water and 1 µl used for real-time qPCR with Qiagen QuantiNova SYBR Green Master Mix (Qiagen) in a StepOnePlus Real-Time PCR System (Applied Biosystems ...
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bioRxiv - Microbiology 2023Quote: ... The pellet was washed once with 0.1% peptone water and subjected to total DNA extraction with a QIAamp PowerFecal Pro DNA Kit (Qiagen, Germany) following manufacturer’s instruction ...
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bioRxiv - Neuroscience 2022Quote: We euthanized groups of 20 to 30 zebrafish larvae by immersion in ice-cold water (below 4°C) and immediately performed RNA extraction using the RNeasy Mini Kit (Qiagen) and reverse transcription using the High-Capacity cDNA Reverse Transcription Kit (Thermo Fisher) ...
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bioRxiv - Genomics 2024Quote: ... The pellet was then dissolved in 0.05 ml deionized water and processed a further purification with the DNeasy Blood and Tissue Kit (No. 69504; Qiagen, Germany). The purified genomic DNA samples were assessed by using the NanoDrop 2000 spectrophotometer (ThermoFisher ...
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bioRxiv - Physiology 2024Quote: ... with 250uL LCMS-grade water and 215uL LCMS-grade MeOH containing 10uL SPLASH lipidomix standard (Avanti #330707) and homogenized in a TissueLyzer (Qiagen) at 4C ...
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bioRxiv - Biophysics 2024Quote: ... the DNA fragments in the samples digested by MNase were purified in sterilized water using the silica membrane spin column (Qiagen). We repaired the purified DNA fragments using the NEBNext® Companion Module for Oxford Nanopore Technologies® Ligation Sequencing (New England Biolabs ...
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bioRxiv - Cell Biology 2024Quote: ... The lysate was then incubated with proteinase K (600 mAU/ml, diluted 1:60 in RNase-free water; Qiagen, Germany) to digest protein ...
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bioRxiv - Genetics 2024Quote: ... The extracted RNAs were resuspended in 100 μL of RNase free water and purified using an RNeasy mini kit (Qiagen) according to the product manual ...
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bioRxiv - Genetics 2024Quote: ... 10 µM of primers and diluted DNA (1:50 dilution in nuclease free water) from input and immunoprecipitated (IP) fraction using Rotor-Gene Q machines (Qiagen). The qPCR program details and the primers used for qPCR validation are given below:
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bioRxiv - Developmental Biology 2020Quote: ... 0.5 µl of diluted template cDNA (1:3, nuclease-free water) per real-time PCR reaction (10 µl – SYBR Green PCR kit, Qiagen, 204143) was used to assay specific transcript abundance (CFX96 Real-Time System ...
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bioRxiv - Microbiology 2020Quote: ... and the pellet containing virions was resuspended in 200 μl UltraPure water and added to the QIAGEN DNeasy PowerSoil Kit bead tubes (QIAGEN, Germany) for DNA extraction according to the manufacturer’s instructions with one exception ...
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bioRxiv - Genetics 2020Quote: ... The RNA pellet was eluted in 15 μL of nuclease-free water and quantified on a Nanodrop 1000 spectrophotometer (NanoDrop Technologies) prior to DNAse treatment with QuantiTect® Reverse Transcription kit (Qiagen). The quality of the RNA was examined by electrophoresis on a 1% agarose gel (Sigma Aldrich) ...
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bioRxiv - Genomics 2021Quote: ... The RNA pellets were subsequently dissolved in an appropriate volume of RNase free water and treated with RNase-Free DNase (Qiagen, 79254) by incubation at room temperature for 10 min to avoid genomic DNA contamination ...
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bioRxiv - Molecular Biology 2021Quote: ... 50 μl of each input and biotinylated RNA was adjusted to 100 μl with nuclease-free water and purified on RNeasy columns (Qiagen #74104) using the modified protocol ...
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bioRxiv - Microbiology 2023Quote: ... two negative PCR controls (further termed as no PCR template controls, NTCs; RNA free water; Cat. No. 760011596, Qiagen, Hilden, Germany) were used ...
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bioRxiv - Microbiology 2023Quote: ... RNA pellets were dissolved in 88 μL of nuclease-free water and subjected to genomic DNA digestion with DNase (Qiagen Inc.). RNA samples were then concentrated using RNA Clean & Concentrator (Zymo Research) ...
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bioRxiv - Plant Biology 2023Quote: ... leaf-discs were collected and ground in autoclaved Milli-Q water using a TissueLyser II (QIAGEN; 45 seconds at 30 Hz). Serial dilutions of the ground tissue were spotted onto low salt Luria Bertani (LB ...
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bioRxiv - Microbiology 2023Quote: ... cells were collected by centrifugation and quickly washed with ice-cold water to remove the methanol and resuspended in RNAlater solution (Qiagen, 76104) for at least 2 hours ...
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bioRxiv - Genomics 2022Quote: ... The RNA pellet was eluted in 15 μL of nuclease-free water and quantified on a Nanodrop 1000 spectrophotometer (NanoDrop Technologies) prior to DNAse treatment with QuantiTect® Reverse Transcription kit (Qiagen). The quality of the RNA was examined by electrophoresis on a 1% agarose gel (Sigma Aldrich) ...
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bioRxiv - Microbiology 2023Quote: ... we used low-binding plastics and added 1 µl of 0.1 ng/µL tRNA carrier (i.e. spike water, Qiagen, Cat. No. 1068337) in each tube with stock eluted DNA ...
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bioRxiv - Molecular Biology 2024Quote: ... 500 ng of RNA were used in the reverse transcription reaction and a mix containing RNAse-free water (Qiagen, Hilden, Germany), RT enzyme ...
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bioRxiv - Genomics 2024Quote: ... The supernatant was then discarded and two 70% ethanol washes performed with the pellet dissolved in 50 μl of DNase free water (Qiagen, Manchester) by incubating overnight at 4°C.
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bioRxiv - Developmental Biology 2024Quote: ... individual offspring and diet samples were homogenized in 200 µL Optima™ water for 2 x 2 min at 25 Hz using a TissueLyser bead mill (QIAGEN), and then an aliquot of methanol (200 µL ...
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bioRxiv - Evolutionary Biology 2024Quote: ... They were placed individually into 2 ml microcentrifuge tubes with 1 ml of distilled water and a stainless-steel bead (Ø 5 mm, and homogenised with a Qiagen TissueLyser LT at a frequency of 30 Hz for one minute ...
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bioRxiv - Developmental Biology 2024Quote: ... Samples were homogenized in 300 µL Optima™ water for 2 x 2 min at 25 Hz using a TissueLyser bead mill (QIAGEN). Another 650 µL Optima™ water was added ...
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bioRxiv - Developmental Biology 2022Quote: ... injected embryos developed to blastocyst stage in culture were collected in 1.5 ul water individually and the whole genome was replicated using a REPLI-g® Mini Kit (Qiagen, Germantown, MD) following the manufacturer’s protocol ...
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bioRxiv - Microbiology 2021Quote: ... ethanol-stored flies were rehydrated in water and transferred to 1.5 ml well plates for homogenisation using a bead beater (Qiagen Tissue Lyzer II). Protein was digested using Proteinase K ...
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bioRxiv - Cell Biology 2021Quote: ... We then suspended the pellet in RNAse-free water and purified it using a Qiagen RNeasy MinElute cleanup kit (Qiagen, cat. #74204) following standard protocols.
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bioRxiv - Cell Biology 2021Quote: ... We then suspended the pellet in RNAse-free water and purified it using a Qiagen RNeasy MinElute cleanup kit (Qiagen, cat. #74204) following standard protocols.
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bioRxiv - Animal Behavior and Cognition 2022Quote: ... Aqueous homogenates of ovarian tissue samples were created by weighing the tissue and adding 4uL of water per mg of tissue and then using a bead beater (TissueLyser II, QIAGEN; Germantown, MD) to homogenize the sample ...
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bioRxiv - Cell Biology 2022Quote: Snap-frozen iBAT samples were homogenized in deionized water (200 µl per 10 mg tissue) using a TissueLyser bead mill (85210, Qiagen, Hilden, Germany). The glycogen content of the homogenates was measured using a Glycogen Assay Kit II (ab169558 ...
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bioRxiv - Pathology 2020Quote: ... The cDNA was first diluted with nuclease-free water and then added to the RT2 SYBR Green ROXTM qPCR Mastermix (Qiagen, Hilden, Germany) according to manufacturer’s protocol ...
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bioRxiv - Systems Biology 2021Quote: ... The liquid was aliquoted into a microcentrifuge tube with 590 µl nuclease-free water and 10 µl Proteinase K (QIAGEN, Hilden, Germany). For RNA extraction from swabs ...
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bioRxiv - Microbiology 2023Quote: ... we dried both washed lichen fragments and wash-water pellets at 65°C and extracted DNA with the DNeasy Plant Mini Kit (QIAGEN, Hilden, Germany).
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bioRxiv - Neuroscience 2023Quote: ... 7 µL of RNase inhibitor and 98 µL nuclease-free water) and the eluted RNA was then concentrated using the RNeasy MinElute Cleanup kit (Qiagen, ref. 74204). The immunoprecipitated RNA was then reverse transcribed and used for qPCR ...
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bioRxiv - Microbiology 2023Quote: ... in-house mock community (25 diverse Gram-positive and Gram-negative bacteria and fungi) and a negative reagent control (nuclease-free water, Qiagen, Hilden, Germany) were included ...
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bioRxiv - Microbiology 2023Quote: ... The RNA samples were resuspended in RNase-free water before clean-up treatment with the RNase-Free DNase Set (Qiagen, Hilden, Germany) following the manufacturer’s protocol for off-column clean-up ...
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bioRxiv - Microbiology 2023Quote: ... where reactions were filled to 100 μl with nuclease-free water and buffer RLT from RNeasy Mini Kit (Qiagen, Venlo, the Netherlands) was added ...
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bioRxiv - Cell Biology 2024Quote: ... Tissues were homogenized in pre-chilled buffer containing an equal amount of water and methanol using TissueRuptor II with a disposable probe (Qiagen, Hilden, Germany). The homogenate was centrifuged at 16,000 ξ g for 15 min at 4°C ...
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bioRxiv - Microbiology 2022Quote: ... Total extracted RNA was resuspended in nuclease free water and one microgram was used for cDNA synthesis using the Quantitect reverse transcription kit (Qiagen, Germantown, MD, USA) following the manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2023Quote: ... 0.5μl of diluted cDNA (1:3 - nuclease-free water) was used as template in 10μl real-time PCR reactions (Qiagen: ‘SYBR Green PCR kit’) to assay specific transcripts (BioRad ...
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bioRxiv - Genetics 2023Quote: ... 4 μL PCR mastermix (made up of 0.75 μl at a concentration of 0.2 μM of each primer, 80 μl ultrapure water, and 250 μl QIAGEN Multiplex PCR mix; QIAGEN Inc. Cat. No. 20614). PCR conditions were as follows ...
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bioRxiv - Genetics 2024Quote: ... 30 μl clarified lysate were mixed with 70 μl RNase free water and RNA isolated using the RNeasy Mini Kit (Qiagen, Germantown, MA, USA) according to the manufacturer’s recommendations ...
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bioRxiv - Biochemistry 2022Quote: ... The isolated RNA was air-dried after precipitation and taken up in 20 μl of RNase-free water followed by a subsequent sample purification using the RNeasy kit (Qiagen, Venlo, The Netherlands). Residual DNA contamination was removed from each sample using the Turbo DNA-free kit (Ambion ...
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bioRxiv - Neuroscience 2022Quote: ... Brain tissues were weighted using an analytical scale and homogenized in 1 ml of deionized water using TissueRuptor II electric homogenizer by Qiagen (Germantown, MA, USA). RO5256390 was extracted using acetonitrile/water 50%/50% (v/v ...
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bioRxiv - Microbiology 2024Quote: ... Isolation of genomic DNA from Sterivex filtered water samples were processed using the DNeasy PowerWater Sterivex Kit (Qiagen Cat no. 14600-50-NF) following manufacturer’s specifications ...