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Citations for Qiagen :
101 - 150 of 303 citations for CdSeTe ZnS Quantum Dots NIR region Water solvent 760nm since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2020Quote: DNA samples were directly PCR amplified for the target region while RNA samples were converted to cDNA using Reverse Transcriptase kit (Qiagen) and PCR amplified ...
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bioRxiv - Genomics 2020Quote: Bisulfite converted DNA was amplified with a region-specific biotinylated/unmodified DNA primer pair (Metabion; Supplemental Table S4) using the PyroMark PCR Kit (Qiagen) according to the manufactures instructions ...
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bioRxiv - Genetics 2021Quote: RNA was extracted from half of a 4 mm biopsy punch for each region of the brain using the Qiagen TissueLyser II for tissue disruption with 5mm stainless steel beads (Qiagen). The RNeasy Plus kit (Qiagen ...
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bioRxiv - Neuroscience 2020Quote: ... from three brain regions (cerebral cortex, hippocampus, and cerebellum) of WT and Cul3+/- mice using the QIAGEN RNAeasy isolation kit (QIAGEN) following manufacturer’s instructions ...
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bioRxiv - Neuroscience 2020Quote: ... Endogenous Mecp2 cDNA was amplified for analysis using a forward primer in the 5’ untranslated region and a reverse primer located in the 3’ untranslated region PCR products were fractionated on a 1% agarose gel and purified using the QIAquick gel extraction kit (Qiagen) before being submitted for Sanger sequence analysis ...
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bioRxiv - Cancer Biology 2020Quote: Genomic DNA was extracted from the same tumor region as the single-cell analyses using the Qiagen AllPrep kit and matched normal blood using DNeasy kit (Qiagen). Briefly ...
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bioRxiv - Cancer Biology 2021Quote: ... Correct and unique amplification of the target regions was verified by agarose gel electrophoresis before purifying PCR products using the QIAquick PCR Purification Kit (Qiagen). For analysis by TIDE ...
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bioRxiv - Microbiology 2022Quote: ... primer sets were designed based on the Medaka-polished contigs and used for the RT-PCR amplification of regions of interest using the OneStep RT-PCR kit (Qiagen) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2020Quote: Functional annotation of genes that are associated with dynamic chromatin regions and/or differential expression with NSC activation was performed using Ingenuity Pathway Analysis (Qiagen Inc. ...
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bioRxiv - Neuroscience 2020Quote: ... Fold enrichment for each primer set was normalized to input and reported relative to a negative control gene desert region (mouse Igx1a (Qiagen). Primers ...
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bioRxiv - Neuroscience 2022Quote: RNA from dissected colon and different brain regions was extracted using the Qiagen RNeasy Plus Universal Mini Kit (Qiagen, 73404). Briefly ...
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bioRxiv - Evolutionary Biology 2022Quote: An aliquot of each region of the NEB5α rnc library was thawed on ice and DNA purified using a QIAprep Spin Miniprep Kit (Qiagen). DNA concentration was estimated by NanoDrop spectrophotometer and verified by gel electrophoresis on a 1% TAE-agarose gel ...
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bioRxiv - Immunology 2021Quote: ... Correct and unique amplification of the target regions was verified by agarose gel electrophoresis before purifying PCR products using the QIAquick PCR Purification Kit (Qiagen). For analysis by TIDE ...
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bioRxiv - Genetics 2021Quote: ... Specific primers were designed to cover approximately 500 base pairs around the mutations and the regions were PCR-amplified using Qiagen Multiplex PCR kit (Qiagen). The PCR products were sequenced with corresponding primers using canonical Sanger sequencing at SeqLab-Microsynth (Göttingen ...
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bioRxiv - Immunology 2020Quote: ... RT-qPCR was performed with a primer set targeting partial regions of the ORF1b gene in the SARS-CoV-2 virus using the QIAGEN OneStep RT-PCR kit (Qiagen) as previously reported (51) ...
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bioRxiv - Developmental Biology 2020Quote: ... (underlined region is the T7 promoter for In vitro transcription) These primers amplified exonic region of Dyrk1b and the product was cleaned using PCR purification kit (Qiagen)and cloned into pGEM®-T Easy Vector Systems (Promega) ...
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bioRxiv - Neuroscience 2023Quote: Samples were collected from the injured cortical region (including affected as well as non-affected brain tissue) and homogenized using the TissueLyser (QIAGEN). Total RNA was extracted with TRI Reagent (Sigma-Aldrich ...
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bioRxiv - Plant Biology 2022Quote: ... coli codon-optimised DNA sequence encoding the conserved extracellular region of AtHMG1 (Uniprot P14891, At1g76490, residues 121-592) was cloned into pQE30 (Qiagen) following an N-terminal His6-tag and tobacco etch virus cleavage site ...
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bioRxiv - Evolutionary Biology 2023Quote: The target regions of the genes of interest were prepared for amplification by using PyroMark PCR Kit (Qiagen, cat. 978903) following the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2023Quote: ... The resulting 1683 bp product spanning the 3’ end of MYO2 upstream of the integration site through the 3’ untranslated region was then isolated using a PCR purification kit (Qiagen), and mutations were confirmed by sequencing using primer 5’- CTCATTTGTGGTGTTTGCTC-3’ ...
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bioRxiv - Molecular Biology 2024Quote: ... The gel region from 200 to 700 bp was excised and DNA was purified by QIAquick Gel Extraction Kit (Qiagen) following the manufacturer’s instructions (we included step 6 in the Quick-Start Protocol for QIAquick ...
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bioRxiv - Microbiology 2020Quote: ... 1 mL was centrifuged and washed twice with nuclease-free water (Qiagen). The cell pellet was resuspended in 500 µL of nuclease-free water ...
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bioRxiv - Microbiology 2021Quote: ... and from water microbiome filters using the PowerWater DNA extraction kit (Qiagen), following the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2020Quote: ... and 2.5 μL of nuclease-free water containing predesigned primers (#249900, Qiagen; QuantiTect Primer Assays IL-6 ...
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bioRxiv - Immunology 2023Quote: ... The RNA was purified in 30 µL of nuclease-free water (Qiagen), and RNA concentration measured on a NanoDrop™ 2000 (Thermo Fisher Scientific) ...
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bioRxiv - Microbiology 2024Quote: ... DNA was eluted in 25 μL of nuclease free water (Qiagen, Germany). Sequencing libraries were prepared from the purified amplicon libraries using the SQK LSK114 kit (Oxford Nanopore Technologies ...
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bioRxiv - Microbiology 2024Quote: ... frozen lung samples were homogenized in ice-cold DNA-free water (Qiagen) to the final concentration of 66.7 mg/ml (w/v ...
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bioRxiv - Microbiology 2024Quote: ... DNA was eluted in 25 μL of nuclease free water (Qiagen, Germany). DNA concentration was measured using Qubit dsDNA HS Assay kit (Thermo Fisher Scientific ...
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bioRxiv - Cancer Biology 2024Quote: ... EV RNA was eluted in 17 μL of nuclease-free water (Qiagen) and assessed for concentration and purity with Bioanalyzer Agilent RNA 6000 Pico Kit.
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bioRxiv - Genetics 2020Quote: ... 1μl of bisulfite converted DNA was used as template to amplify target genomic regions using specific primers (one biotinylated) with the PyroMark PCR kit (Qiagen, #978703), following the recommended conditions (annealing temperature 56°C) ...
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bioRxiv - Neuroscience 2020Quote: Total RNA was extracted from fresh-frozen tissue from four brain regions (motor cortex, frontal cortex, hippocampus, and cerebellum) using the AllPrep DNA/RNA Mini kit (Qiagen, Germany) according to manufacturer’s instructions ...
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bioRxiv - Neuroscience 2021Quote: ... Total RNA was extracted from brain regions of adult mice using an RNeasy Plus Mini Kit (Cat#74134, Qiagen, Germantown, USA), in accordance with the manufacturer’s instructions ...
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bioRxiv - Microbiology 2020Quote: ... the variable region genes from each single cell were amplified in a reverse transcriptase polymerase chain reaction (RT-PCR: QIAGEN, Germany) using a cocktail of sense primers specific for the leader region and antisense primers to the Cγ constant region for heavy chain and Cκ and Cγ for light chain ...
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bioRxiv - Genetics 2020Quote: Approximately 50 mm3 of tumor tissue from each region was used for genomic DNA extraction using the QIAamp DNA Mini Kit (Qiagen, Germany) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2020Quote: ... cDNA products were then used in nested PCR for heavy/light chain variable region amplification with HotStarTaq Plus DNA Polymerase (QIAGEN # 203643) and specific primer sets described previously 34,35 ...
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bioRxiv - Genomics 2023Quote: The bacterial 16S rRNA V4 variable region was amplified for 5 cycles with the HotStarTaq Plus Mastermix Kit (Qiagen, Hilden, Germany). Samples were purified with Ampure XP beads (Beckman Coulter ...
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bioRxiv - Molecular Biology 2023Quote: Extracted normal and tumor regions were ground in liquid nitrogen and RNA extracted using the RNeasy RNA extraction kit (Qiagen 74104) then analyzed using a 2200 Tapestation Analyser (Agilent) ...
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bioRxiv - Immunology 2023Quote: For protein production a pT350 plasmid encoding for the construct of interest with a BiP signal sequence in the N-terminal and a double strep-tag in the C-terminal region was co-transfected with a puromycin resistant plasmid (pCoPuro) (61) with effectene reagent (Qiagen, 301425) following manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2024Quote: ... The GPR52 guide target regions were then amplified by PCR using the following primers and purified with the QIAquick PCR Purification Kit (Qiagen #28104). GPR52-1 F ...
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bioRxiv - Microbiology 2020Quote: ... DNA plus a negative extraction control (nuclease-free water Qiagen, Venlo, The Netherlands) were sent to the Research Technology Support Facility at Michigan State University (Michigan ...
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bioRxiv - Microbiology 2020Quote: ... RNA was dissolved in nuclease free water and treated with DNase I (Qiagen) for 15 min at 37°C ...
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bioRxiv - Cell Biology 2022Quote: ... The purified RNA was eluted in nuclease-free water (Qiagen, product number 120114) and was quantified using NanoDrop A260 absorbance as well as the Qubit RNA HS assay (Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2020Quote: Liver tissues (~100mg) were homogenized in 1.2mL of water using a TissueLyser (Qiagen) for 5min ...
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bioRxiv - Molecular Biology 2023Quote: ... 30 µl of nuclease-free water (Qiagen, Germany, Center Mainz, Cat No: 129114) was added and the pellet was resuspended ...
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bioRxiv - Microbiology 2023Quote: ... DNA was eluted in 35 µl of Nuclease-free water (Qiagen, Hilden Germany).
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bioRxiv - Neuroscience 2024Quote: ... The pellet was resuspended in 30–50 μl RNase-free water (Qiagen, 129114), incubated at 65°C for 5 minutes ...
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bioRxiv - Cell Biology 2024Quote: ... sterile water) and 30 µL of 20 mg/mL Proteinase K (Qiagen, 19131) were added to the 30×106 cells ...
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bioRxiv - Neuroscience 2021Quote: ... Between 0.1 and 1.0 μg (region dependent) of normalized sample were used for cDNA synthesis using QuantiTect Reverse Transcription Kits (Qiagen, Valencia, CA, USA).
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bioRxiv - Genomics 2020Quote: ... Additional biopsy sections were collected from the same regions and at two distinct areas of the tumor were placed in Allprotect Tissue Reagent (Qiagen, Hilden, Germany) and stored at 4° C for DNA extraction and evaluated histopathologically ...
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bioRxiv - Cancer Biology 2020Quote: ... Genomic DNA was extracted from zebrafish melanoma tumors (Tg[mitfa:BRAFV600E];p53-/- 17,40) and from a normal region of the same fish using the Qiagen DNeasy Blood and Tissue kit (Qiagen, Germantown, MD, #69504). The DNA was fragmented and hybridized to the array ...