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Citations for Qiagen :
2751 - 2800 of 6611 citations for SARS CoV 2 Spike Glycoprotein S1 His tag Insect Cells since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2024Quote: ... Cell pellets were resuspended in 450 µl RLT buffer (QIAGEN, Hilden, Germany) with 1% β-mercaptoethanol ...
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bioRxiv - Developmental Biology 2024Quote: ... media was removed and cells were lysed with Qiazol (Qiagen, Cat. #79306). RNA extraction was performed according to manufacturer’s guidelines ...
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bioRxiv - Microbiology 2024Quote: As outlined by the REPLI-g cell WGA & WTA handbook from Qiagen, 8 µL of lysis buffer was added to 13 µL of the final RNA fraction of interest and was incubated at 24 °C for 5 minutes followed by 95 °C for 3 mins and cooled down at 4 °C ...
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bioRxiv - Microbiology 2023Quote: ... Cell-free supernatant was lysed with AVL buffer (Qiagen cat. No 19073) and RNA was extracted with QIAmp Viral RNA Mini Kit (Qiagen 52904).
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bioRxiv - Immunology 2024Quote: ... 10,000 cells were lysed in 600uL RLT lysis buffer (Qiagen Cat#79216) + 1% BME ...
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bioRxiv - Genetics 2023Quote: Total RNA from cultured cells was isolated using RNeasy RNA kit (Qiagen) and processed with TURBO DNA-free kit (Thermo Fisher AM1907) ...
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Mitochondrial Apolipoprotein MIC26 is a metabolic rheostat regulating central cellular fuel pathwaysbioRxiv - Cell Biology 2023Quote: ... RNA isolation from cell pellets was performed using RNeasy Mini Kit (Qiagen) including DNase digestion according to the manufacturer’s protocol ...
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Mitochondrial Apolipoprotein MIC26 is a metabolic rheostat regulating central cellular fuel pathwaysbioRxiv - Cell Biology 2023Quote: ... cells were lysed and homogenized in urea buffer with a TissueLyser (Qiagen) and supernatants were collected after centrifugation for 15 min at 14,000 x g and 4°C ...
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Mitochondrial Apolipoprotein MIC26 is a metabolic rheostat regulating central cellular fuel pathwaysbioRxiv - Cell Biology 2023Quote: Total RNA was extracted from cell pellets using RNeasy Mini Kit (Qiagen) according to the manufacturer’s protocol ...
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bioRxiv - Immunology 2023Quote: ... cells were incubated with goat anti-rat beads (QIAGEN, cat. no. 310107). For intracellular staining ...
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bioRxiv - Biochemistry 2024Quote: ... were expressed in SF9 cells and purified by affinity (Ni-NTA, Qiagen) followed by size exclusion (Superdex 200 ...
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bioRxiv - Neuroscience 2023Quote: Dissected tissues in the cell aggregates were lysed with Buffer RLT (Qiagen) containing 1% 2-mercaptoethanol ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... All stable cell line plasmid transfections were conducted with Effectene (Qiagen, #301425) as per manufacturer’s instructions ...
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bioRxiv - Pathology 2023Quote: Total RNA was extracted from cells using the RNeasy Mini Kit (Qiagen). cDNA synthesis ...
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bioRxiv - Immunology 2022Quote: ... RNA was extracted from 1 × 106 cells using the RNeasy kit (Qiagen), and the heavy and light chain sequences of the murine hybridomas were by obtained using the mouse Ig-primer set (69831 ...
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bioRxiv - Cell Biology 2023Quote: Total RNAs were isolated from cells using the RNeasy Mini Kit (Qiagen) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2023Quote: ... cells were transfected with dsRNA using the Effectene Transfection reagent (301427, QIAGEN) and incubated for 72 h ...
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bioRxiv - Microbiology 2023Quote: ... 1.5 ml of the cells were treated with RNAprotect Bacteria Reagent (QIAGEN). Total RNA was extracted using GeneJET RNA purification kit (Thermo scientific) ...
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bioRxiv - Microbiology 2023Quote: Cells were lysed and total RNA extracted using the RNeasy kit (Qiagen) according to the manufacturer’s instructions ...
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bioRxiv - Genetics 2023Quote: ... cells were lysed with AllPrep DNA/RNA Mini Kit (Qiagen, Hilden, Germany) or RIPA for assays as described below.
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bioRxiv - Neuroscience 2023Quote: ... RNA was extracted from pelleted cells using RNeasy Micro Kit (QIAGEN 74004), followed by cDNA synthesis (AmfiSure ONE PCR Master Mix P7000-050 ...
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bioRxiv - Systems Biology 2023Quote: ... with the cell-lysate first being homogenized through a QIAshredder column (Qiagen), per the manufacturer’s directions ...
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bioRxiv - Bioengineering 2023Quote: RNA was extracted from HCT116 cells using the RNeasy Mini Kit (QIAGEN) following the manufacturer’s instructions ...
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bioRxiv - Microbiology 2023Quote: ... then resuspended in 3 mL of RNAprotect for bacterial cells (Qiagen, #76506). Planktonic cultures were similarly pelleted and resuspended in RNAprotect ...
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bioRxiv - Microbiology 2023Quote: ... Harvested cells were fixed overnight in 5 mL RNA Protect reagent (Qiagen) after incubation with compounds ...
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bioRxiv - Cancer Biology 2023Quote: ... or FACS-sorted cell populations were lysed in RLT lysis buffer (Qiagen) + 1% β-mercapto-ethanol and RNA was purified using the RNeasy kit (Qiagen ...
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bioRxiv - Pathology 2023Quote: ... The cell pellet was re-suspended in 350μl of buffer RLT (Qiagen), and RNA extracted with RNeasy Plus Mini Kits (Qiagen ...
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bioRxiv - Cancer Biology 2023Quote: ... Cells were washed with PBS and lysed using buffer RLT plus (Qiagen) supplemented with 1% beta-mercaptoethanol (Sigma-Aldrich ...
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bioRxiv - Cancer Biology 2023Quote: RNA was isolated from cultured cells using the RNeasy mini kit (Qiagen) following the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2023Quote: ... Cell lysate supernatants were applied to packed Ni-NTA beads (QIAGEN; 30210). After washing with 20 column volume of lysis buffer ...
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bioRxiv - Cancer Biology 2023Quote: ... and genomic DNA was isolated (Blood & Cell Culture DNA Midi Kit, Qiagen). DNA was PCR amplified with Illumina-compatible primers (P7 and P5 mix) ...
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bioRxiv - Neuroscience 2023Quote: Total RNA was extracted from cells using the RNeasy Mini Kit (Qiagen) according to the manufacturer instructions and retrotranscribed with the QuantiTect Reverse Transcription Kit (Qiagen) ...
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bioRxiv - Neuroscience 2023Quote: RNA was isolated from cell pellets using the RNeasy Mini Kit (Qiagen) following manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2023Quote: Total RNA was extracted from cells with RNeasy Plus Mini kit (Qiagen) according to the manufacturer’s instructions and quantified using Qubit RNA BR Assay kit on Qubit 2.0 Fluorometer (Thermo Fisher Scientific) ...
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bioRxiv - Neuroscience 2023Quote: ... Cells were resuspended with 1 mL of QIAzol (QIAGEN Inc.; Germantown, MD) and transferred to tissue homogenizing CKMix tubes 2mL (Bertin Instruments ...
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ALS iPSC-derived microglia and motor neurons respond to astrocyte-targeted IL-10 and CCL2 modulationbioRxiv - Neuroscience 2023Quote: RNA was isolated from cell pellets using the RNeasy Mini Kit (Qiagen) following manufacturer’s instructions ...
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bioRxiv - Microbiology 2023Quote: ... DNA from infected cells was extracted using the QIAamp DNA-minikit (Qiagen) according to manufacturer’s instructions ...
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bioRxiv - Systems Biology 2023Quote: ... Total RNA was isolated from the cells using the RNeasy kits (Qiagen) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2023Quote: ... Cells were transfected with dual-luciferase and Renilla ERE reporter vectors (Qiagen), according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2023Quote: ... The sorted cells were deep frozen in 2x TCL buffer plus (Qiagen) plus 1% beta-mercaptoethanol.
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bioRxiv - Genetics 2023Quote: ... and K were extracted using the miRNeasy Tissue/Cells Advanced Kits (Qiagen). DNase treatment is done using the TURBO™ DNA-free (Invitrogen) ...
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bioRxiv - Cell Biology 2023Quote: ... Genomic DNA (gDNA) was isolated using the Gentra Puregene Cell Kit (QIAGEN). Integrated sgRNA regions were amplified by PCR using custom primers (Supplementary Table 4 ...
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bioRxiv - Cancer Biology 2023Quote: ... RNA was extracted from cell culture using the RNeasy Mini Kit (Qiagen) according to the manufacturer’s protocol ...
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bioRxiv - Immunology 2023Quote: RNA was extracted from sorted cells using the RNeasy mini kit (Qiagen) or RNAqueous Micro Kit (Ambion) ...
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bioRxiv - Genomics 2023Quote: ... were used to homogenize cells and the AllPrep DNA/RNA kit (Qiagen) was used to purify RNA and gDNA ...
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bioRxiv - Systems Biology 2023Quote: ... with the cell-lysate first being homogenized through a QIAshredder column (Qiagen), per the manufacturer’s directions ...
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bioRxiv - Cell Biology 2023Quote: RNA was extracted from cells and tissue using RNeasy Mini Kit (Qiagen) with on-column DNA digest (Qiagen ...
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bioRxiv - Biochemistry 2023Quote: Total RNA was extracted from cells using RNeasy Plus mini kits (Qiagen). RNA concentration was measured by a Qubit fluorescence assay (Thermo Fisher ...
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bioRxiv - Biochemistry 2023Quote: For RNA extraction cells were scraped and lysed using QIAshredders (#79654, QIAGEN), RNA was extracted by means of the Qiagen RNeasy Mini kit (#74104 ...
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bioRxiv - Biochemistry 2023Quote: ... plasmids were transiently co-transfected into HEK293T cells using PolyFect (Qiagen 301105) according to the manufacturer’s instructions ...