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Citations for Qiagen :
2651 - 2700 of 6611 citations for SARS CoV 2 Spike Glycoprotein S1 His tag Insect Cells since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2020Quote: ... WT A431 cells were transduced with Cignal Lenti Myc Reporter (Qiagen, USA) expressing Firefly luciferase under E-Box promoter and CMV-Renilla luciferase under CMV promoter ...
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bioRxiv - Molecular Biology 2020Quote: mRNA was isolated from live cells using the Qiagen RNeasy kit (Qiagen) and cDNA synthesis was performed using the iScript cDNA synthesis kit (BioRad) ...
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bioRxiv - Neuroscience 2020Quote: ... of Raw 264.7 cells at P3 using the RNeasy Mini Kit (Qiagen). 500 ng of the extracted RNA was used to synthesize cDNA using a High Capacity cDNA RT kit (Applied Biosystems ...
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bioRxiv - Immunology 2021Quote: RNA was extracted from cells using RNeasy Plus Mini Kit (Qiagen, 74136) according to manufacturer’s instructions ...
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bioRxiv - Immunology 2021Quote: Total RNA was isolated from cells using the RNeasy isolation kit (Qiagen), and cDNA prepared using the Quantitech Reverse Transcription kit (Qiagen) ...
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bioRxiv - Microbiology 2021Quote: ... cells and purified using QIAprep spin and midi kits (Qiagen, Valencia, CA). Bacterial genomic DNA was extracted from L ...
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bioRxiv - Cell Biology 2019Quote: RNA was extracted from cells using the RNeasy Mini Kit (Qiagen; 74104) using spin technology ...
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bioRxiv - Cell Biology 2019Quote: ... MD.[54] The constructs were transfected into HeLa cells using Fugene (Qiagen).
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bioRxiv - Cell Biology 2019Quote: ... cells were reverse transfected with 10nM siRNA (final concentration) using HiPerfect (Qiagen) according to manufacturer’s instruction.
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bioRxiv - Cancer Biology 2019Quote: ... Cells were harvested in cold PBS and lysed in RLT buffer (Qiagen). RNA quality and integrity were verified with the Bioanalyzer system (Agilent) ...
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bioRxiv - Cell Biology 2019Quote: ... TDP-43 KO and rescue cell lines using the RNeasy kit (Qiagen). 400 ng of total RNA was reverse transcribed using the iScript cDNA Synthesis kit (Bio-Rad) ...
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bioRxiv - Genomics 2021Quote: ... Cells were washed with phosphate buffered saline and harvested in QIAzol (Qiagen) to preserve RNA ...
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bioRxiv - Developmental Biology 2021Quote: Total RNA molecules were extracted from cells with RNeasy-Mini Kits (Qiagen). Protocol of 3’ DGE RNA sequencing was performed as previously described in (Picarda et al ...
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bioRxiv - Genetics 2020Quote: Total RNA was extracted from the cells with RNeasy mini kit (Qiagen) according to the manufacturer’s instructions ...
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bioRxiv - Genomics 2021Quote: ... and RNA were extracted from cells using Qiagen AllPrep kit (Qiagen, 80204) following the manufacturer’s protocol ...
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bioRxiv - Immunology 2021Quote: ... 1,000 cells were sorted into 300 μl of RLT buffer (Qiagen; 79216) containing 1% 2-ME and snap frozen in a dry ice – ethanol bath ...
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bioRxiv - Cell Biology 2021Quote: ... RNA was extracted from cell pellet using the RNeasy Mini kit (Qiagen). cDNA was then synthesized using the QuantiTect Reverse Transcription Kit (Qiagen ...
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bioRxiv - Evolutionary Biology 2020Quote: ... Total RNAs from cells were isolated using RNeasy Plus Micro Kit (QIAGEN) according to the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2021Quote: ... RNA was extracted from cell pellets using the RNeasy Mini kit (Qiagen), as per the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2020Quote: Total RNA was isolated from cells using the RNeasy Mini Kit (Qiagen). Reverse transcription was performed with random primers using the SuperScript II First-Strand Synthesis System (Invitrogen) ...
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bioRxiv - Cell Biology 2020Quote: ... Cells were transfected using siRNAs targeting MAD2 sequence 5′-AGAUGGAUAUAUGCCACGCTT-3′ (Qiagen), pools of siRNAs targeting the TP53 sequence 5’-GAAAUUUGCGUGUGGAGUA-3’ ...
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bioRxiv - Biochemistry 2020Quote: ... Protein was purified from cell supernatants using Ni-NTA resin (Qiagen, Germany), the eluted fractions containing S protein were pooled ...
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bioRxiv - Biochemistry 2021Quote: ... Protein was purified from cell supernatants using Ni-NTA resin (Qiagen, Germany), the eluted fractions containing S protein were pooled ...
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bioRxiv - Physiology 2021Quote: ... MicroRNAs were extracted from cells and tissues using miRNeasy Mini Kit (Qiagen). Reverse transcription was done with TaqMan MicroRNA Reverse Transcription Kit and quantified using a TaqMan MicroRNA assay kit and TaqMan Universal Master Mix II ...
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bioRxiv - Neuroscience 2021Quote: ... and transiently transfected in S2 cells using Effectene (QIAGEN, catalog no. 301425). Cells were collected three days post-transfection ...
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bioRxiv - Neuroscience 2020Quote: ... cells were pelleted and RNA was extracted using RNeasy Micro Kit (QIAGEN) according to manufacturer’s instructions ...
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bioRxiv - Immunology 2021Quote: ... 106 cells per sample were processed following the miRNeasy micro kit (Qiagen). After RNA quantification ...
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bioRxiv - Immunology 2021Quote: RNA was isolated from cells as using the RNeasy micro kit (Qiagen) for PC samples or the RNeasy mini kit (Qiagen ...
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bioRxiv - Cell Biology 2020Quote: ... Cells were then pelleted and suspended in 125 µl P1 buffer (Qiagen), that was then mixed with 125 µl P2 buffer (Qiagen ...
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bioRxiv - Molecular Biology 2021Quote: RNA was extracted from cell samples with the RNeasy mini kit (Qiagen) by following the manufacturer’s instructions ...
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bioRxiv - Immunology 2021Quote: ... The sorted cells were deep frozen in 2x TCL buffer plus (Qiagen) plus 1% beta-mercaptoethanol.
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bioRxiv - Cancer Biology 2019Quote: RNA was extracted from cells using the RNeasy RNA extraction kit (Qiagen), followed by the iScript™ cDNA Synthesis kit (Bio-Rad ...
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bioRxiv - Cancer Biology 2021Quote: RNA from PCa cells was isolated by RNeasy Mini kit Plus (Qiagen) according to the manufacturer’s recommendations ...
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bioRxiv - Biophysics 2020Quote: ... The cell-free extract was bound to pre-equilibrated Ni2+-NTA (Qiagen) matrix for 3 h ...
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bioRxiv - Cell Biology 2021Quote: ... Sorted cells were immediately centrifuged and resuspended in Qiazol (Qiagen, Cat# 217004) for total RNA isolation.
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bioRxiv - Cell Biology 2020Quote: Drosophila adult flies or Drosophila S2 cells were homogenised using QIAshredder (QIAGEN) and total RNA was extracted with RNeasy Mini Kit (QIAGEN ...
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bioRxiv - Cell Biology 2020Quote: ... S2 cells were transfected with indicated constructs using Effectene transfection reagent (QIAGEN) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2020Quote: ... using the Blood and Cell Culture DNA MaxiKit (QIAGEN, Cat No. 13362). sgRNA sequences was amplified by PCR as described (Doench et al. ...
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bioRxiv - Cell Biology 2020Quote: RNA was isolated from cell pellets with the RNeasy kit (Qiagen, 74104) and quantified with a Nanodrop spectrophotometer (Thermo Fisher Scientific) ...
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bioRxiv - Physiology 2020Quote: Cells and tissues were lysed in TRIzol using a TissueLyser II (Qiagen) and RNA was extracted using a PureLink RNA kit (catalog no ...
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bioRxiv - Evolutionary Biology 2020Quote: ... Total RNA from cells was extracted using RNeasy Plus Mini Kit (QIAGEN) following the manufacturer’s protocol ...
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bioRxiv - Genetics 2021Quote: ... for less than 1×104 cells or the RNeasy kit (Qiagen #74004) otherwise ...
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bioRxiv - Immunology 2020Quote: ... human sputum cells or mouse lung tissue using an RNeasy kit (Qiagen). 2μg was utilised for cDNA synthesis using the Omniscript RT kit (Qiagen) ...
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bioRxiv - Molecular Biology 2021Quote: ... DAPI-/NPHS1+ cells were collected in RLT buffer (RNeasy, Qiagen, Germantown, Maryland) for further processing.
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bioRxiv - Immunology 2020Quote: ... Total RNA was extracted from cells using the RNeasy Micro Kit (Qiagen). mRNA libraries were prepared using the KAPA stranded mRNA-seq kit by Duke Sequencing and Genomic Technologies Shared Resource facility ...
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bioRxiv - Immunology 2020Quote: ... or CD4+ T cells was extracted using the RNeasy micro kit (Qiagen) followed by cDNA synthesis using the iScript cDNA synthesis kit (BioRad) ...
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bioRxiv - Immunology 2021Quote: ... RNA was isolated from cells using Qiagen RNeasy Plus Mini Kit (Qiagen). cDNA was synthesized using ∼1μg of DNase-treated RNA with random hexamers and SuperScript III reverse transcriptase (Invitrogen) ...
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bioRxiv - Microbiology 2021Quote: ... cells were lysed and RNA extracted using the Rneasy Mini Kit (Qiagen). Knockdown of target genes was assessed by qPCR ...
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bioRxiv - Immunology 2020Quote: ... Plates were spun down and cell pellets were resuspended in Qiazol (Qiagen) for RNA extraction ...
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bioRxiv - Molecular Biology 2021Quote: ... Genomic PCR of cell line material used 25 ng purified DNA (Qiagen), followed by direct sequence analysis of the product (Macrogen).