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Citations for Qiagen :
2751 - 2800 of 3437 citations for Fipronil 3 Cyano Pyrazole 3 4 5 13C4 99%; 3 Cyano 5 15N2 98% 100 Ug Ml In Methanol since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2020Quote: ... The replicated amplicons for each sample were combined into a single reaction of 100 μl and purified using a QIAquick PCR Purification Kit (Qiagen, 28104) and quantified on the Qubit Fluorometer ...
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bioRxiv - Plant Biology 2019Quote: ... 50,000 protoplasts in a volume of 100 μL were transformed with 20 μg of each plasmid (2 μg/μL) purified using the Plasmid Maxi kit (Qiagen, Germany). One batch of protoplasts was treated with an equivalent amount of water and used as the negative (untransformed ...
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bioRxiv - Neuroscience 2019Quote: ... total RNA was extracted from 200 μl conditioned medium or 100 μl foetal plasma using the miRNeasy Mini Kit and the miRNeasy Serum/Plasma Kit (Qiagen, Germany). microRNA expression levels were analysed using the nCounter Rat v1.5 miRNA Expression Assay (NanoString Technologies ...
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bioRxiv - Genomics 2019Quote: ... To each sample 100 μl PBS and 250 μl Ethanol was added and RNA was purified using the Qiagen RNeasy mini kit (Qiagen 74104). RNA was next treated with DNaseI (NEB ...
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bioRxiv - Genetics 2019Quote: ... 50 µM LNA oligo in 100 µL serum free media is mixed with 12 µL HiPerfect transfection reagent (Qiagen, Valencia, CA) and incubated for 20 min at room temperature ...
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bioRxiv - Cancer Biology 2020Quote: ... cells were washed twice in PBS and incubated for 20 min at 54 °C (60 min at 52 °C for tissue sections) in mercury locked-nucleid acid (LNA) miRNA ISH Buffer 80 nM hybridiziation buffer (100 nM for tissue section) (Qiagen, 339450). Digoxigenin labeled miRCURY LNA probes (HSA-MIR-10B-5P ...
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bioRxiv - Systems Biology 2021Quote: Genomic DNA for sequencing was isolated with the the Qiagen 100/G kit according to the manufacturer’s description (Qiagen, Hilden, Germany) and library preparation and sequencing was done as described previously using Illumina Miseq sequencing (Illumina ...
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bioRxiv - Immunology 2021Quote: ... a new empty 200 µL filtered tip was inserted to top channel outlet and 100 ul RNase easy lysis buffer (Qiagen, #74034) was used to lyse epithelial cells by quickly pressing and releasing the micropipette plunger 3 times ...
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bioRxiv - Microbiology 2022Quote: ... An aliquot of EDTA-treated whole blood (100 μl) was diluted with 600 μl of AVL inactivation buffer (Qiagen, Hilden, Germany), and RNA was extracted using a Viral RNA mini-kit (Qiagen ...
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bioRxiv - Microbiology 2021Quote: ... metagenomic DNA was extracted from a soil sample taken from the Northwestern University campus (coordinates 42.05662, −87.674247) using a DNeasy PowerSoil Kit (Qiagen, cat#12888-100). Quantification of DNA for all experiments was performed using a QuantiFluor ONE dsDNA system (Promega ...
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bioRxiv - Physiology 2021Quote: Approximately 100 ng/µL of total DNA was extracted from 20 mg tissue (QiAMP DNA mini kit Qiagen, Valencia, CA, USA). DNA from bone marrow was extracted from the bone marrow suspension and centrifuged for 10 min at 2000 g ...
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bioRxiv - Plant Biology 2021Quote: ... and salt treated samples (100 mM, 250 mM and 500 mM NaCl) was extracted using RNeasy Plant Mini Kit (Qiagen, Germany). RNA quality and quantity assessment ...
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bioRxiv - Microbiology 2019Quote: ... The replicated amplicons for each sample were combined into a single reaction of 100 ul and purified using a QIAquick PCR Purification Kit (Qiagen, 28104) and quantified on the Qubit Fluorometer ...
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bioRxiv - Genomics 2020Quote: ... Dehydrated samples were stored at −80 °C until resuspension in 25 μl or 100 μl of Buffer EB (QIAGEN, Venlo, Netherlands).
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bioRxiv - Plant Biology 2020Quote: ... For expression analysis total RNA was extracted from 100 mg of freeze-dried leaf tissue of chilli and tomato lines using RNeasy plant kit (Qiagen, USA) according to manufacturer’s protocol ...
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bioRxiv - Genomics 2020Quote: ... Frozen muscle was pulverized in liquid nitrogen and genomic DNA was extracted using a Genomic Tip 100/G kit (Qiagen, USA). DNA quantity was measured using a NanoDrop ND-8000 spectrophotometer and a Qubit dsDNA BR Assay kit (Invitrogen ...
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bioRxiv - Molecular Biology 2021Quote: ... 50 μl of each input and biotinylated RNA was adjusted to 100 μl with nuclease-free water and purified on RNeasy columns (Qiagen #74104) using the modified protocol ...
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bioRxiv - Immunology 2022Quote: ... DNA synthesis reaction was carried out in 100 μl using 10 pmol of each primer with HotStarTaq Plus DNA Polymerase (Qiagen, 203603). Purified dsDNA products were amplified with 10 pmol of each primer in a 100 μl total reaction volume ...
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bioRxiv - Genetics 2022Quote: ... Size selection was also used for purification of 100 bp-200 bp PCR products by QIAquick Gel Extracton (28706, QIAGEN, Germany).
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bioRxiv - Genetics 2022Quote: ... The aqueous phase was mixed with an equal volume of 100% ethanol and transferred to an RNeasy MinElute spin column (Qiagen, 74104) for purification according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2022Quote: ... The pellet was rinsed with 70% ethanol and dried at room temperature before dissolving in 100 μL of RNase-free DNase set (Qiagen 79254) and incubated at room temperature for 10 minutes to remove DNA ...
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bioRxiv - Molecular Biology 2022Quote: ... total RNA was extracted from a pool of 100–120 adult females per line using the RNeasy plus mini kit (Qiagen, Belgium) with 4 replicates for strains MAR-ABi and JP-RRi and 5 replicates for strains ROS-ITi and SOL-BEi ...
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bioRxiv - Molecular Biology 2023Quote: ... the other half of tumor tissues were lysed using lysis buffer (0.1% Triton X-100) and a TissueLyser homogenizer (Qiagen, Hilden, Germany). DNA for PCR was isolated from 50 µl of tissue lysate using the Maxwell RSC tissue DNA Kit (Promega ...
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bioRxiv - Microbiology 2023Quote: ... DNA extraction was performed from 200 mg of three independent samples using the MO BIO’s Powersoil DNA isolation kit procedure (Qiagen 12888-100) as described previously (von Gastrow et al. ...
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bioRxiv - Genomics 2023Quote: ... The sample was then centrifuged at 15000 rpm for 10 min and the pellet was resuspended in 100 μl buffer EB (Qiagen, 19086). For each sample of a 96-well plate ...
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bioRxiv - Bioengineering 2023Quote: ... samples were suspended in RNeasy kit Lysis Buffer and β-mercaptoethanol (1:100 v/v) and homogenized using QIAshredder columns (Qiagen). Samples loaded on spin columns were treated with DNase (Qiagen ...
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bioRxiv - Microbiology 2023Quote: ... Bacterial genomic DNA was isolated from pure culture using QIAGEN Genomic-tip 100/G following the manufacturer’s instruction of (Qiagen, Valencia, CA). The Seqwell plexWell LP384 Library Preparation Kit and Native Barcoding Kit 24 V14 (SQK-NBD112.24 ...
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bioRxiv - Cancer Biology 2023Quote: MiR libraries were generated from 100 -150 ng of cell using the QIAseq miRNA Library Kit according to the protocol (Qiagen, Germany). Library quality control and concentrations were determined using a High Sensitivity D1000 ScreenTape with TapeStation Analysis Software A.02.02 (Agilent Technologies ...
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bioRxiv - Microbiology 2024Quote: DNA was extracted using a modified form of the QIAGEN DNeasy PowerWater kit (QIAGEN, Hilden, DEU, cat. no. 14900-100-NF) method ...
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bioRxiv - Cell Biology 2024Quote: Cell pellets or lung lobes from individual mice were snap frozen in 350µl of 1:100 β-mercaptoethanol in the RLT Lysis Buffer from the RNAeasy mini plus Kit (Qiagen, 74134). Cell samples were thawed and vortexed while lung samples were homogenized for total RNA extraction intended for qPCR or bulk RNA-seq ...
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bioRxiv - Developmental Biology 2024Quote: Bisulfite conversion was performed on 100 ng of genomic DNA using the EpiTect Fast DNA Bisulfite kit from Qiagen (Cat.# 59824) and DNA libraries were constructed using the NUGEN Ovation RRBS Methyl-seq System (Cat.# 0353 ...
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bioRxiv - Cell Biology 2024Quote: ... all samples were diluted to the same RNA concentration and 250 ng of RNA from each sample spiked with 100 ng mouse RNA to control reaction efficiency were reverse transcribed using the miScript II RT kit (Qiagen, 218161) with HiFlex Buffer according to manufacturer’s instructions ...
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bioRxiv - Neuroscience 2021Quote: ... ∼200 picked cells were stored in RLT buffer (Cat. No. 79216 – 220 mL, Qiagen) with 1% 2-Mercaptoethanol (M6250-100ML ...
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bioRxiv - Molecular Biology 2021Quote: ... 300 mM of NaCl and 2 μL of RNase A (0.5 mg / mL) (Qiagen) were added to the collective eluates which were incubated at 65 °C overnight ...
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bioRxiv - Immunology 2021Quote: ... Periplasmic fraction was incubated with 1 mL pre-washed Ni-NTA agarose (30230, Qiagen) for 1 h at 4°C ...
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bioRxiv - Microbiology 2019Quote: ... the supernatant was applied to 2 mL of pre-washed Ni-NTA resin (Qiagen) at 4°C ...
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bioRxiv - Microbiology 2020Quote: ... DNA was extracted from 1 ml of the slurry using a commercial kit (Qiagen).
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bioRxiv - Biophysics 2021Quote: ... 2 g/ml Biotinylated Anti-His Antibody (Penta-His Biotin Conjugate; Qiagen; No. 34440) in T50 buffer was introduced into flow cells at 50 μl/min ...
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bioRxiv - Neuroscience 2020Quote: ... Samples were then treated with RNase A (2 mg/ml in water, Qiagen, 19101) for 2 hours at 37°C ...
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bioRxiv - Biophysics 2021Quote: The supernatant was mixed with 2 mL Ni-NTA resin (Ni-NTA agarose; Qiagen) pre-equilibrated with buffer A200 (50 mM Tris pH 7.6 ...
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bioRxiv - Cell Biology 2021Quote: ... before adding it to 2 ml of Strep-Tactin superflow resin (Qiagen, Hilden, Germany) which was pre-equilibrated with 10 ml lysis buffer ...
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bioRxiv - Molecular Biology 2022Quote: rhGAA secreted from D532 was purified using 1 mL Ni-NTA agarose resin (Qiagen) according to the manufacturer’s instructions and as described previously [27] with slight modification ...
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bioRxiv - Plant Biology 2022Quote: ... Cells were sorted into 1.5 ml buffer RLT from RNeasy Plant Mini-Kit (Qiagen) containing 1% v/v β-mercaptoethanol ...
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bioRxiv - Microbiology 2022Quote: ... The resulting supernatant was supplemented with 1 mL pre-equilibrated Ni-NTA resin (Qiagen) and 10 mM imidazole and stirred for 30 min at 4 °C ...
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bioRxiv - Microbiology 2022Quote: ... Clarified lysate was added to 1 mL of washed Ni-NTA bead slurry (Qiagen) and incubated for 1 hour at 4°C ...
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bioRxiv - Microbiology 2022Quote: ... three cocoa beans were homogenised in 30 ml of RNAprotect (Qiagen, Venio, The Netherlands) in a 50 ml falcon and vortexed for 30 seconds to disrupt the mucilaginous pulp from the seeds ...
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bioRxiv - Microbiology 2021Quote: ... The resulting supernatant was supplemented with 0.75 mL pre-equilibrated Ni-NTA resin (Qiagen) and 20 mM imidazole and stirred for 30 min at 4 °C ...
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bioRxiv - Biochemistry 2020Quote: ... The lysate was gently shaken with 0.5 ml of Ni-NTA agarose suspension (Qiagen) for 1 h at 4 °C and transferred to a filter column where the liquid was drained ...
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bioRxiv - Biophysics 2022Quote: ... and the supernatant was added to 0.5 mL (bed volume) Ni-NTA resin (Qiagen) equilibrated in wash/lysis buffer ...
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bioRxiv - Genomics 2022Quote: ... DNA was further treated with 50 µg/ml of RNase enzyme (Qiagen, Hilden, Germany) for 1 h followed by phenol/chloroform extraction ...