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Citations for Qiagen :
2601 - 2650 of 3437 citations for Fipronil 3 Cyano Pyrazole 3 4 5 13C4 99%; 3 Cyano 5 15N2 98% 100 Ug Ml In Methanol since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genomics 2020Quote: High-molecular-weight DNA from white (20025) and yellow fat (70346) individuals was extracted from liver tissue using Genomic-tips (G/100) from Qiagen, and fragments smaller than 25Kb were progressively depleted using a Short Read Eliminator kit (Circulomics ...
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bioRxiv - Genetics 2019Quote: ... We extracted total RNA from 100 mg of flower buds right before flowering of all the parental accessions using RNeasy Plant Mini Kit (QIAGEN) with RNase-free DNase I (Invitrogen) ...
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bioRxiv - Genomics 2019Quote: ... and 100 mg of ground leaves were used for DNA extraction using the DNeasy® Plant Mini Kit (Qiagen, Valencia) according to the instructions of the manufacturer ...
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bioRxiv - Microbiology 2019Quote: ... Total environmental DNA was isolated from this 100 μL cell suspension by the QIAamp DNA Mini Kit (Qiagen, Hilden, Germany), following the manufacturer’s protocol.
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bioRxiv - Biochemistry 2021Quote: ... The his-tagged 3C protease was removed by flowing and washing off cleaved transporter protein over two 100 µl beds of Ni-NTA Superflow resin in tandem (Qiagen). Eluent was concentrated using 100 kDa cut-off Amicon Ultra filters (Millipore-Sigma).
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bioRxiv - Bioengineering 2021Quote: ... Exosome samples were treated with 1 µL RNAse A/T1 per 100 µL sample for 30 min at 37°C and lysed in 650 µL of QIAzol (Qiagen). To monitor the RNA recovery percentage as a function of the isolation procedure ...
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bioRxiv - Microbiology 2021Quote: ... 3-20 μl of individual PCR products (adjusted based on estimated relative amplicon concentration) were combined into 100 μl sub-pools and purified using an UltraClean 96 PCR Cleanup Kit (Qiagen). Blank extractions ...
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bioRxiv - Microbiology 2020Quote: DNA was extracted from ∼100 mg of cecal contents using the MoBio UltraClean Soil DNA extraction kit (Qiagen, Carlsbad, CA) following the manufacture’s protocol ...
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bioRxiv - Neuroscience 2021Quote: ... SH-SY5Y cells were treated either with 100 μM cycloheximide (CHX) or DMSO for 6 hours (41) before harvesting the RNA through RNeasy Minikit (Qiagen). Reverse transcription was performed using RevertAid cDNA synthesis kit (Thermo) ...
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bioRxiv - Plant Biology 2021Quote: RNA was extracted from ∼100 mg of root tissue and DNase treated using the RNeasy plant mini kit and RNase free DNase kit (Qiagen) according to the manufacturer’s instructions ...
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bioRxiv - Plant Biology 2021Quote: ... Total RNAs were prepared using 100 mg of homogenized tissues and RNeasy Plant Mini kit with on-column DNase treatment (Qiagen). Libraries for RNA-Seq were prepared from 1 µg of total RNAs with the TruSeq Stranded mRNA Library Prep Kit (Illumina ...
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bioRxiv - Cell Biology 2021Quote: Transgenic parasites were generated by electroporation of sorbitol-synchronized ring-stage parasites with 50-100 μg of purified plasmid DNA (Qiagen). To select for the plasmids pArl-PfCentrin1-GFP and p3-NLS-L3-mCherry ...
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bioRxiv - Neuroscience 2019Quote: ... Library fragments in the range of 100-800 bp were size-selected using agarose gel electrophoresis followed by DNA gel extraction (QIAGEN). Recovered DNA was further cleaned and concentrated using a column (Zymo Research) ...
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bioRxiv - Cancer Biology 2020Quote: Cells were seeded in 96-well plates (2 × 104 cells/well) and reverse-transfected with 100 ng/well of Cignal ISRE-Luc reporter (Qiagen) using Lipofectamine 3000 ...
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bioRxiv - Microbiology 2021Quote: ... Individual blood-fed female flies were separated into Eppendorf tubes containing 100 uL of DNA lysis buffer (Gentra Puregene Tissue Kit, Qiagen) and stored at -20°C.
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bioRxiv - Molecular Biology 2022Quote: ... Biotin-labelled RNA was eluted with 100 μl elution buffer (100 mM DTT in nuclease-free water) and cleaned up with the RNeasy MinElute Cleanup kit (QIAGEN), adjusting the amount of ethanol to capture RNA < 200 nucleotides in length ...
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bioRxiv - Plant Biology 2019Quote: ... total RNA was extracted from 100 mg of apical parts of primary inflorescences of exo70a2 and WT plants using the RNeasy kit (Qiagen). Prior to reverse transcription ...
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bioRxiv - Microbiology 2019Quote: ... Undigested debris were spun down 3 min 10000 rpm and the supernatant was supplemented with 500 μl of 100% ethanol prior loading onto RNeasy mini columns (Qiagen). From this step onwards ...
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bioRxiv - Bioengineering 2019Quote: ... the cells were washed once with 0.1% PBSA and incubated with 50 μL of a 1:100 dilution of anti-penta-His Alexa 647 antibody (Qiagen) for 10 minutes on ice ...
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bioRxiv - Genomics 2020Quote: ... The ethanol was then poured out and the pellet was air-dried for 15 minutes before resuspension in 50 to 100 μL of DNA Hydration Solution (Qiagen). DNA was sequenced with paired end Illumina sequencing at 30x coverage.
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bioRxiv - Evolutionary Biology 2021Quote: ... Most DNA samples for ONT sequencing were extracted from 100 female flies from each strain using the Blood and Cell Culture DNA Mini Kit (Qiagen) following manufacturer’s instructions with small modifications (Table S2 ...
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bioRxiv - Genetics 2021Quote: High-quality genomic DNA of yeast for sequencing was extracted using the QIAGEN Blood & Cell Culture Kit with 100/G or 20/G Genomic-tips (QIAGEN) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2021Quote: ... These plasmids were purified and DNA extracted using QIAGEN Plasmid Midi Kit (100) (cat. n° 12145, QIAGEN, Valencia, CA, USA), according to manufacturer’s instructions.
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bioRxiv - Biochemistry 2021Quote: Genomic DNA was isolated from 2 clones of each strain corresponding to 50 OD600 units with the Genomic DNA Buffer Set and Genomic-tip 100/G (QIAGEN) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2020Quote: ... 1.5X volumes of 100% ethanol was used to precipitate RNA prior to column loading and RW1 buffer was substituted with RWT (Qiagen). For cDNA synthesis of mRNAs ...
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bioRxiv - Molecular Biology 2021Quote: ... 50 μl each input and purified RNA was adjusted to 100 μl with nuclease-free water and purified on RNeasy columns (Qiagen) using the modified protocol as described (Duffy and Simon ...
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bioRxiv - Microbiology 2021Quote: DNA was extracted from 100 µl of harvest of culture-enriched samples using DNeasy Blood & Tissue kit (Qiagen, Hilden, Germany) as previously described [22] ...
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bioRxiv - Immunology 2020Quote: ... cleaning were used to purify 100 μl of pooled cDNA with final elution in 15 μl PCR-grade H2O (Qiagen). After quantification with Qubit dsDNA HS assay (Thermofisher) ...
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bioRxiv - Genomics 2022Quote: ... the genomic DNA of the Nichi01 strain was isolated from the silk glands of two male fifth-instar larvae using Genomic-tips 100/g and Genomic DNA Buffer Set (QIAGEN). Long-read sequencing for genome assembly was performed using continuous long-read (CLR ...
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bioRxiv - Genomics 2022Quote: ... The mixture was then centrifuged for 20 min at 12 000 x g and the clarified lysate transferred to an appropriate buffer QBT-equilibrated QIAGEN Genomic-tip 100/G column (Qiagen), after which the column was washed three times with 7 ml Buffer QC and HMW DNA was eluted with 5 ml Buffer QF ...
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bioRxiv - Genomics 2022Quote: ... Genomic DNA for MLPA and ddPCR assays was extracted from 100 mg leaves with a DNeasy Plant Mini Kit (Qiagen), according to manufacturer’s protocol ...
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bioRxiv - Genetics 2022Quote: Total RNA was isolated from 50 to 100 μl of packed worms from wild type and brd-1(null) using the RNeasy Mini Kit (74104; Qiagen) and QIAshredder (79654 ...
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bioRxiv - Plant Biology 2022Quote: ... solutions for 0- and 30-minutes Total RNA was extracted from 100 mg of frozen and ground tissue from mock and treated seedlings using the RNeasy Plant Mini Kit (Qiagen) according to the manufacturer’s protocol (31) ...
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bioRxiv - Plant Biology 2022Quote: ... Proteins were induced with 100 μM IPTG at 12°C for 16 hrs and purified on a Ni2+ column according to manufacturer protocols (Qiagen). Proteins were eluted using 125 mM imidazole ...
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bioRxiv - Neuroscience 2022Quote: ... Tubes were spun for 15 minutes and the RNA in the aqueous phase was precipitated with 100% ethanol and transferred into an RNeasy column (Qiagen). 30μl of RNA per sample were collected and the concentration and purity were determined using a Nanodrop 1000 (Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2022Quote: ... bead beating was performed on 100 mg of cecal contents for 10 min using a tissue lyser (Qiagen, Germantown, MD). Remaining steps for DNA isolation were performed as per manufacturer’s instruction ...
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bioRxiv - Plant Biology 2024Quote: RNA was extracted from approximately 100 mg of frozen samples according to a modified RNeasy Plant Mini Kit (Qiagen, Germany) procedure [21] ...
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bioRxiv - Immunology 2024Quote: ... 100-200 μm thick sections were cut and used to extract total RNA using the RNeasy Mini Kit (Qiagen, USA). The RNA integrity number (RIN ...
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bioRxiv - Microbiology 2024Quote: DNA was extracted in random order from the faecal slurries (n=484) using DNeasy PowerLyzer PowerSoil kit (Qiagen, 12855-100) and the V3-region of the 16S rRNA gene was PCR amplified using 0.2 µl Phusion High-Fidelity DNA polymerase (ThermoFisher Scientific ...
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bioRxiv - Evolutionary Biology 2024Quote: ... alphenor females that were homozygous for the h or H alleles using the QIAgen GenomicTip G-100 kit (QIAgen, USA). Extractions followed the manufacturer’s instructions ...
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bioRxiv - Microbiology 2024Quote: ... samples were resuspended in 100 μl TE buffer pH 8 and RNA extraction performed according to the RNeasy Mini Kit (Qiagen) protocol with few modifications ...
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bioRxiv - Neuroscience 2024Quote: ... DRG and paw skin RNA was extracted using a RLT buffer:2-mercapto ethanol mixture in a 100:1 ratio/RNeasy (Qiagen) RNA mini kit according to the manufacturer’s instructions and spinal cord and spleen RNA was extracted using TRizol (Invitrogen)/RNeasy RNA mini kit ...
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bioRxiv - Cell Biology 2024Quote: ... falciparum 3D7 ring stage parasites were transfected with 100 μg of purified plasmid DNA (Plasmid Maxi Kit, Qiagen, Valencia, CA) by electroporation (310 V ...
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bioRxiv - Immunology 2024Quote: ... The upper aqueous layer was mixed with an equal volume of 100% ethanol and processed using miRNeasy Mini Kit (Qiagen) according to the manufacturer’s instructions with the following modifications ...
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bioRxiv - Genomics 2024Quote: ... The bacterial cells were harvested by centrifugation and the DNA extraction was performed by the used of Genomic-tip 100/G column (Qiagen) according to the manufacturer’s protocol ...
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bioRxiv - Immunology 2024Quote: Fecal pellets were collected and stored at −80°C until DNA extraction with DNeasy PowerLyzer PowerSoil Kit (Qiagen; 12855-100). Fecal Rg abundance was determined with Rg-specific 16S rDNA primers ...
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bioRxiv - Microbiology 2022Quote: Genomic DNA (gDNA) was extracted from stationary broth culture using genomic DNA-Tip 100/G system and buffer set (Qiagen) following the manufacturer’s instruction ...
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bioRxiv - Cell Biology 2023Quote: ... Twenty-four hours later 100,000 cells were transferred to a tube containing 100 µl of RLT buffer (Qiagen, Hilden, Germany). Cells were stored at –80 °C until further processing ...
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bioRxiv - Cell Biology 2023Quote: ... into parasites already ectopically expressing NLS-mCherry (previously provided by Markus Ganter (Simon et al., 2021a)) with 50–100 μg of purified plasmid DNA (QIAGEN). Plasmid precipitation was done prior to transfection as stated above ...
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bioRxiv - Microbiology 2023Quote: A total of 100 ng total RNA was used for library preparation via the QIAseq miRNA Library Kit (#331502, Qiagen) according to manufacturer’s recommendations ...