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Citations for Qiagen :
2551 - 2600 of 3263 citations for 2 3 5 Dioxo 4 aza tricyclo 5.2.1.0*2 6* dec 8 en 4 yl propionic acid since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genetics 2021Quote: Genomic DNA was extracted from an aliquot of 3 × 106 cells using the Gentra Purgene Kit (Qiagen). The genomic region surrounding the CRISPR/Cas9 target site (741 bp ...
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bioRxiv - Bioengineering 2022Quote: ... messenger RNA (mRNA) combined from at least 3 gels were isolated with an RNeasy Mini Kit (Qiagen) and then reverse transcribed into complementary DNA (cDNA ...
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bioRxiv - Immunology 2022Quote: ... DNA was extracted from frozen cell pellets (3×107 cells; Blood & Cell Culture DNA Maxi Kit, Qiagen) per manufacturer’s instructions ...
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bioRxiv - Plant Biology 2022Quote: Total RNA was extracted (n=3) from the frozen endosperm tissue using the RNeasy PowerPlant kit (Qiagen). An on-column DNase digest was incorporated during the extraction ...
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bioRxiv - Molecular Biology 2022Quote: ... RNA (>200nt) was extracted from 400 testes of ≤3 days old flies using miRNeasy Mini column (QIAGEN). For the first replicate ...
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bioRxiv - Microbiology 2024Quote: ... MRU2687-3 and ZH548 viral stocks were extracted using the QIAmp Viral RNA kit (Qiagen; Courtaboeuf, France) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2023Quote: Small interfering RNA was obtained to knockdown the expression of ARSB and galectin-3 (Qiagen, Germantown, MD). Effects on mRNA were determined by QRT-PCR ...
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bioRxiv - Cancer Biology 2023Quote: Total RNA from patient material (n=3) and organoids (xxx) was extracted (RNeasy™ Mini Kit, Qiagen). To obtain cDNA ...
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bioRxiv - Biochemistry 2023Quote: ... The supernatant from the second spin was incubated with 3 mL of Ni-NTA agarose beads (Qiagen) for 30 minutes at 4°C in the cold room ...
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bioRxiv - Cell Biology 2024Quote: ... Tissues were homogenized for five 3-minute cycles of 30 shakes/sec using a TissueLyser II (Qiagen) and transferred to an RNase free microfuge tube (Ambion ...
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bioRxiv - Immunology 2024Quote: ... we transfected the 293T cells at 3 million cells/10 cm dish with Effectene transfection reagent (Qiagen) following manufacturer guidelines ...
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bioRxiv - Cancer Biology 2024Quote: The FASTQ file was analyzed by QIAseq UPX 3’ Transcriptome Primary Quantification tool (QIAGEN GeneGlobe analysis website) to generate gene expression matrix ...
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bioRxiv - Cell Biology 2024Quote: ... RNase-A was degraded by the addition of proteinase-K (3 mAU/mL, 19131, Qiagen, Hilden, Germany) and inverting the tube briefly ...
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bioRxiv - Microbiology 2021Quote: ... was modified by a custom-added biotin residue at its 5’-end (Qiagen). Hybrids of the CoV-2 RNAs with the probe were detected with a goat anti-biotin antibody conjugated to 10 nm gold particles (BBI international).
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bioRxiv - Cell Biology 2020Quote: ... mRNA samples were concentrated to ≤ 5 µl by MinElute column (QIAGEN, Cat. 74204). For generation of RNA-seq libraries ...
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bioRxiv - Immunology 2021Quote: RNA was isolated from ~5 x 105 cells using RNeasy mini kits (Qiagen), typically yielding 100-400 ng RNA ...
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bioRxiv - Plant Biology 2021Quote: ... Samples were homogenized using 5 mm steel beads and a Tissuelyser II (Qiagen) operated at 30 Hz for 5 min ...
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bioRxiv - Immunology 2020Quote: ... 5 U/ml dispase (Stemcell) and 50 mg/ml DNase I mix (Qiagen) in complete RPMI1640 medium ...
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bioRxiv - Molecular Biology 2021Quote: ... genomic DNA was isolated from 5 million cells using the DNAeasy kit (Qiagen). 4ug gDNA was digested with NlaIII or MseI ...
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bioRxiv - Cell Biology 2020Quote: ... 20 μl of 10% SDS and 5 ul of Proteinase K (Qiagen #19131) were added to each sample ...
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bioRxiv - Evolutionary Biology 2022Quote: ... 10x CoralLoad PCR buffer and 5 µl of TopTaq Master Mix 2x (Qiagen). An initial denaturation cycle of 94°C for 6 min was followed by 35 cycles of 94°C for 45 s ...
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bioRxiv - Biochemistry 2022Quote: ... The cleared lysate was applied to a 5 mL Ni-NTA cartridge (Qiagen) and the column was washed in two steps with lysis buffer supplemented with 25 and 50 mM imidazole ...
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bioRxiv - Physiology 2022Quote: ... mRNA samples were concentrated to ≤ 5 µl by MinElute column (QIAGEN, Cat. 74204). For generation of RNA-seq libraries ...
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bioRxiv - Microbiology 2022Quote: ... The cleared lysate was applied to a 5 mL Ni-NTA cartridge (Qiagen). The column was washed with 3 column volumes of lysis buffer and proteins were eluted stepwise with lysis buffer supplemented with 50 and 250 mM of imidazole ...
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bioRxiv - Microbiology 2022Quote: ... The cleavage products were passed through a 5 mL Ni-NTA cartridge (Qiagen) and the column was washed with 5 column volumes of lysis buffer supplemented with 50 mM imidazole to remove the cleaved tag and the TEV protease ...
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bioRxiv - Cell Biology 2020Quote: ... 20 μl of 10% SDS and 5 ul of Proteinase K (Qiagen #19131) were added to each sample ...
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bioRxiv - Biophysics 2020Quote: ... This periplasmic extract was then incubated with 5 ml Ni-NTA beads (Qiagen) at 4°C for 15 minutes and the resin slurry was packed on an open chromatography column ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... Protein was isolated from the lysate using 5 mL Ni-NTA resin (Qiagen) pre-equilibrated with lysis buffer and then washed with 15 column volumes (CV ...
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bioRxiv - Zoology 2021Quote: ... 0.1 μL of 5 U/μL Taq DNA Polymerase (QIAGEN, Valencia, CA, USA), 0.2 μL of U19 fluorescent dye ...
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bioRxiv - Immunology 2020Quote: ... miRNA inhibitors (miRCURY LNA miRNA power inhibitor with or without 5’-FAM, Qiagen) were added directly to culture media at the time of activation and were taken up by the cells by spontaneous translocation across the cell membrane ...
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bioRxiv - Biochemistry 2021Quote: ... The supernatant was applied to three 5 ml Ni-NTA Superflow cartridges (Qiagen). The resin was washed with 20 mM HEPES pH 8.0 ...
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bioRxiv - Biochemistry 2021Quote: ... The sample was applied to a 5 ml Ni-NTA Superflow cartridge (Qiagen) in order to remove the uncleaved fusion protein and His6-TEV protease ...
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bioRxiv - Biochemistry 2021Quote: ... The cleared lysate was applied to a 5 mL Ni-NTA cartridge (Qiagen) and the column was washed in two steps with lysis buffer supplemented with 25 and 50 mM imidazole ...
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bioRxiv - Genetics 2021Quote: ... The supernatant was treated for 5 minutes at room temperature with RNAse (Qiagen) and then centrifuged again ...
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bioRxiv - Bioengineering 2021Quote: ... total RNA was extracted from 5 organoids using the RNeasy Plus MiniKit (Qiagen). Purity was determined byA260–A280 ...
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bioRxiv - Genomics 2020Quote: ... Pre-capture libraries with less than 100 ng of double-stranded cDNA (n=5; PT0017_Qiagen_20ng_XTHS, PT0017_Covaris_20ng_XTHS, PT0017_Qiagen_20ng_Illumina ...
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bioRxiv - Microbiology 2023Quote: ... RNA was extracted from 5 ml samples using the miRNAeasy mini kit (Qiagen), treated with TURBO DNase (Ambion ...
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bioRxiv - Molecular Biology 2024Quote: ... 5 µg/mL DNase I (AppliChem) and 200 µg/mL RNase A (Qiagen) using 4 cycles of 15 s sonification at 30% duty cycle and 30% output control ...
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bioRxiv - Biochemistry 2024Quote: ... and the supernatant was mixed with 5 ml of Ni-NTA agarose (QIAGEN) pre-equilibrated with solubilization buffer for 60 min ...
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bioRxiv - Microbiology 2024Quote: ... 37°C in 5% CO2 and purified with a RNeasy mini kit (Qiagen) according to the protocol for Gram-positive bacteria ...
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bioRxiv - Cell Biology 2023Quote: ... The supernatant was loaded onto a 5 mL StrepTactin Superflow Plus catridge (Qiagen) using a 1 mL/min flow rate and washed with Cyclin B1 wash buffer and eluted with wash buffer containing 2 mM desthiobiotin ...
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bioRxiv - Molecular Biology 2023Quote: mRNA was extracted from ∼5×106 cells using the miRNeasy Mini Kit (QIAGEN). Library preparation and sequencing was conducted by City of Hope Integrative Genomics Core ...
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bioRxiv - Cell Biology 2023Quote: ... Total RNA from 5×107 cells was extracted using RNeasy mini kit (Qiagen). One μg total RNA was used for cDNA synthesis using Quantinova Reverse Transcription kit (Qiagen) ...
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bioRxiv - Molecular Biology 2023Quote: ... The samples are next loaded onto a 5 mL Hitrap S column (Qiagen) pre-equilibrated with 50 mM HEPES pH 7.5 ...
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bioRxiv - Immunology 2022Quote: ... homogenized using 5-mm steel beads and a tissue lyser (Qiagen TissueLyser II) 6x at 25 Hz for 15s ...
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bioRxiv - Cancer Biology 2022Quote: A 100 µL slurry of 5% Ni-NTA magnetic beads (Qiagen, part# 36113) was placed in 12 1.5 mL Eppendorf tubes and the tubes were placed in a magnetic stand and the supernatant was removed ...
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bioRxiv - Microbiology 2022Quote: ... (5) hybrid libraries were then eluted in 40μL of elution buffer (Qiagen, 19086).
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bioRxiv - Microbiology 2023Quote: ... or a 1.5 ml tube with 5 volumes of RNAlater (Qiagen, Valencia, CA) and preserved at -80 °C for RT-PCR.
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bioRxiv - Microbiology 2023Quote: ... containing 500 µl PBS and one 5 mm steel bead (Qiagen, Hilden, Germany) prior to bead-beating for 20 seconds to disrupt the cuticle ...
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bioRxiv - Physiology 2024Quote: ... and 5 μl of QuantiFast SYBR Green RT-PCR Master Mix (Qiagen, Germany). Reverse transcription was initiated with a hold at 50 °C for 10 min (cDNA synthesis) ...