Labshake search
Citations for Qiagen :
2451 - 2500 of 2997 citations for 2 1H Imidazol 1 yl 6 methyl 4 pyrimidinemethanamine since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Molecular Biology 2021Quote: HIV-1 viral RNA was extracted from 20-50 ul of plasma using QIAamp Viral RNA mini kit (Qiagen). qRT-PCR was performed using a TaqMan Fast Virus 1-Step Master Mix ...
-
bioRxiv - Genomics 2020Quote: ... the final clean-up was performed at 0.9:1 ratio of beads to PCR products and elution again done in 16μl EB (Qiagen, Germany). Samples were diluted to 8pM final concentration before sequencing on an Illumina Miseq (2×25nt).
-
bioRxiv - Pathology 2020Quote: Total RNA was extracted from the BALF sample of patient 1 using the RNeasy Plus Universal Mini Kit (Qiagen) following the manufacturer’s instructions ...
-
bioRxiv - Molecular Biology 2021Quote: HOS cells were cotransfected with a Rev/Env-encoding pSVIIIenv plasmid and a Tat-encoding plasmid at a 1:0.125 ratio using the Effectene transfection reagent (Qiagen). At 48 h after transfection ...
-
bioRxiv - Neuroscience 2020Quote: ... qPCR was performed on cDNA dilutions of 1/20 in the presence of 7.5-pmol primers with QuantiTect SYBR Green PCR Master Mix (Qiagen) on a Lightcycler 480 (Roche) ...
-
bioRxiv - Microbiology 2021Quote: Virus isolation was performed on tissues following homogenization in 1 mL DMEM using a TissueLyser (Qiagen, Germantown, MD, USA) and inoculating Vero E6 cells in a 96 well plate with 200 µL of 1:10 serial dilutions of the homogenate ...
-
bioRxiv - Neuroscience 2021Quote: ... Reverse transcription was performed according to the manufacturer’s recommendations on 1 μg of RNA with the QuantiTect Reverse Transcription Kit (Qiagen), which includes a genomic DNA–removal step ...
-
bioRxiv - Microbiology 2020Quote: ... A total of OD600 = 0.3 in 1 ml of cells was pelleted and gDNA was extracted using DNeasy Blood & Tissue kit (Qiagen) using the Gram-negative bacterial protocol followed by quantification using Qubit ...
-
bioRxiv - Plant Biology 2021Quote: ... ABI1 and ABI2 with primers listed in Supplemental Table 1 using the Rotor Gene SYBR Green PCR Kit (Qiagen) according to manufacturers’ protocol in a Rotor gene Q cycler (Qiagen) ...
-
bioRxiv - Microbiology 2020Quote: ... Fastq files were mapped to KSHV genome (GenBank accession no. AP017458 for SPEL, and NC_003409 for BCBL-1) by CLC Genome Workbench (version 14, Qiagen), and the coverage data were obtained using Integrative Genomics Viewer (version 2.50 ...
-
bioRxiv - Microbiology 2020Quote: Each dissected compartment (legs and thoraxes) was homogenized individually 3 x 1 minute at 30 Hertz using TissueLyser (Qiagen) and glass beads (#11079110 ...
-
“A Proteogenomic workflow reveals distinct molecular phenotypes related to breast cancer appearance”bioRxiv - Systems Biology 2020Quote: ... after which a second volume of 400μL a 1% β-mercaptoethanol in RLT buffer (AllPrep DNA/RNA Minikit, Qiagen) was added after steel bead removal ...
-
bioRxiv - Plant Biology 2020Quote: ... 200 mg of freeze-dried and ground roots were mixed with 1.6 mL of 1 M perchloric acid and homogenized in a TissueLyser II (Qiagen) in microtubes containing 6 glass beads of 2.8 mm in diameter ...
-
bioRxiv - Microbiology 2020Quote: ... Crystals of Brd4(BD1) with YFV-C (1-10) K4ac peptide were grown from a solution containing 0.2 M sodium chloride (QIAGEN), 0.1 M Tris (pH 7.0 ...
-
bioRxiv - Biochemistry 2022Quote: ... 10% glycerol and 1 mM DTT) and recombinant TrcP was purified from clarified lysates using Ni-NTA resin (Qiagen) and gravity chromatography ...
-
bioRxiv - Genetics 2022Quote: Total RNA was isolated from PWS and control INS-1 lines grown as above (7.5 mM glucose) by Trizol harvest and miRNeasy (Qiagen) column purification ...
-
bioRxiv - Cancer Biology 2022Quote: ... The pellet was washed three times with ice-cold PBS and suspended in 1 ml TRIzol™ reagent (QIAGEN). Instead ...
-
bioRxiv - Molecular Biology 2022Quote: ... 1×104 cells of each sample were lysed in 100 µl of RLT Plus Lysis Buffer (Qiagen, Hilden, Germany) supplemented with 1% 2-mercaptoethanol ...
-
bioRxiv - Microbiology 2022Quote: ... and stored pellets at −80 °C for <1 week before RNA extraction using RNeasy Protect Bacteria Mini Kit (Qiagen) according to the manufacturer’s instructions (protocol #5) ...
-
bioRxiv - Cancer Biology 2022Quote: 1 μg of bulk genomic DNA was extracted from gastric tumors using the DNeasy Blood and Tissue Kit (Qiagen) and WES was conducted and sequenced by BGI ...
-
bioRxiv - Cancer Biology 2022Quote: ... 1 μg of bulk genomic DNA was extracted from gastric tumors using the DNeasy Blood and Tissue Kit (Qiagen) and sonicated using the Covaris instrument ...
-
bioRxiv - Microbiology 2020Quote: ... genomic DNA from 1-5 million total CD4+ T cells was isolated using the Gentra Puregene Cell Kit (Qiagen) or phenol-chloroform and the DNA concentration was measured by Qubit High Sensitivity Kit (Thermo Fisher Scientific) ...
-
bioRxiv - Neuroscience 2021Quote: ... miR-67 (miR control) or empty vector at a ratio of 1:3 using a lipofection protocol (Effecten, Qiagen) and processed at DIV15 ...
-
bioRxiv - Microbiology 2020Quote: Airway epithelia cultured on 6.5-mm Transwell membranes were washed and treated for 1 minute at RT with RLT buffer (Qiagen) with 1% β-mercaptoethanol (Sigma-Aldrich) ...
-
bioRxiv - Cancer Biology 2020Quote: ... cancer-associated fibroblasts (CAFs) and C666-1 cells using RNeasy Mini kit per the manufacturer’s instructions (Qiagen, Hilden, Germany). RNA integrity number (RIN ...
-
bioRxiv - Microbiology 2021Quote: ... PCR products were confirmed by agarose gel electrophoresis using 0.8-1% agarose and purified using PCR purification kit (Qiagen). In case of NS1 gene ...
-
bioRxiv - Microbiology 2021Quote: ... with sequences and concentrations listed in Table 1 in combination with QuantiTect Multiplex PCR Kit (Qiagen, Germantown, MD, USA). PMAxx (Biotium ...
-
bioRxiv - Microbiology 2022Quote: Frozen tissue was partially thawed and submerged in lysis buffer containing 1% β-mercaptoethanol and 0.5% Reagent DX (Qiagen) before tissues were homogenised together with TissueRupture (Qiagen) ...
-
bioRxiv - Developmental Biology 2022Quote: ... Oocytes and embryos were then washed with D-PBS containing 1 mg/ml polyvinylpyrrolidone (PBS-PVP) and transferred into 50 μl droplets of 0.1% protease (Qiagen) to remove the zona pellucida ...
-
bioRxiv - Genetics 2022Quote: ... The lysates were cleared by centrifugation at 10,000 xg for 30 min and added to 1 ml of Ni-NTA agarose (Qiagen) pre-equilibrated with the same lysis buffer ...
-
bioRxiv - Microbiology 2020Quote: ... adjusted to pH 8.0 using 1M Tris-HCl pH 8.0 and incubated at room temp for 1 h with Ni-NTA Agarose beads (Qiagen). Beads were washed twice with PBS ...
-
bioRxiv - Bioengineering 2020Quote: ... each SeraCare panel member was diluted 1:10 in human plasma to a final volume of 100μL and processed by QIAGEN QIAamp viral mini kit ...
-
bioRxiv - Systems Biology 2020Quote: DNA was isolated from CAMA-1 and CAMA-1_ribociclib_resistant cells using DNeasy Blood & Tissue Kit (Qiagen, Cat. No.: 69504) according to the manufacturer’s protocol ...
-
bioRxiv - Physiology 2021Quote: ... feces were homogenized in 1 mL 80% (v/v) methanol using metal beads (2.4 mm diameter, Askubal) in a tissue lyzer (Qiagen) for 1 min at 30 Hz ...
-
bioRxiv - Biochemistry 2020Quote: ... 12 000 rpm 45 min) and the supernatant was loaded onto a column containing NiNTA resin (1 mL, Qiagen) pre-equilibrated with buffer A (10 mL) ...
-
bioRxiv - Molecular Biology 2021Quote: The solubilized protein was incubated for 1 hour with washed and pre-equilibrated Strep-Tactin Superflow plus resin (Qiagen). The resin was then loaded into an Econo-Column Chromatography Column (Biorad ...
-
bioRxiv - Molecular Biology 2021Quote: ... the membrane was incubated with mouse primary antibody α-His (1:5000, Monoclonal mouse Tetra-His antibody, Qiagen, #34670) in 2.5% BSA in PBS-T overnight at 4°C ...
-
bioRxiv - Microbiology 2021Quote: ... Five days later 1×107 cells were harvested and DNA isolated for sequencing (QIAamp DNA Blood Midi Kit, Qiagen). For each experimental condition (mock ...
-
bioRxiv - Genetics 2020Quote: ... The PCR product was separated on a 1 % agarose gel and purified using the QIAquick Gel Extraction Kit (Qiagen). 60 ng of each probe were labeled with dATP [α-32P] (DECAprime kit II ...
-
bioRxiv - Immunology 2020Quote: Lung tissue from individual mice were homogenized in 1 mL of TRIsure reagent with TissueLyser II (Qiagen No. 853000) and as per the manufacturer’s instructions and samples were centrifuged for 15 min at 12,000 x g at 4°C to remove cellular debris ...
-
bioRxiv - Biochemistry 2020Quote: ... The amplified genes were then electrophoresed in 1% agarose gel followed by purification using PCR purification kit (Qiagen, USA). Both purified genes and plasmids were digested with Nde I and Not I prior to ligation using the T4 DNA ligase (New England Biolabs ...
-
bioRxiv - Genomics 2021Quote: ... and an aliquot of 300-500uL was immediately mixed at 1:2.76 volume ratio with fluid from a Paxgene blood RNA tube (Qiagen), whilst the remainder was stored on ice for flow cytometry analysis ...
-
bioRxiv - Biochemistry 2021Quote: The LRH-1-10CA-TIF2 complex was concentrated to 5 mg/mL and screened using the Classics screen (Qiagen) and a Phoenix Liquid Handler (Art Robbins Instruments ...
-
bioRxiv - Developmental Biology 2020Quote: ... RNA was extracted from DF-1 cells and HH27 whole chick heads using the RNeasy Plus Kit (Qiagen, 74136) following the manufacturer’s directions ...
-
bioRxiv - Cell Biology 2020Quote: ... and A10 encoding constructs or 1 μg Shh constructs with or without 0.5 μg Scube2 by using Polyfect (Qiagen). Cells were grown for 2 days or 3 days for Disp−/− rescue experiments at 37°C with 5% CO2 in DMEM containing 10% fetal calf serum and penicillin-streptomycin (100 μg/mL) ...
-
bioRxiv - Microbiology 2021Quote: ... PCR products were analyzed on a 1% agarose gel and bands gel purified using QIAquick gel extraction kit (Qiagen). Samples were sequenced via Sanger sequencing (Genewiz ...
-
bioRxiv - Molecular Biology 2020Quote: ... 15uL of the sample was de-crosslinked overnight at 65°C with 85ul of decrosslinking buffer (0.1M NaHCO3, 1% SDS) before clean up using QiaQuick PCR clean up kit from Qiagen.
-
bioRxiv - Molecular Biology 2020Quote: ... cells were washed with PBS and the pellet was resuspended in 1 ml PBS containing RNAse A (Qiagen, 19101) at a final concentration of 0.2 mg/ml ...
-
bioRxiv - Immunology 2020Quote: ... were homogenised in a Precellys 24 homogeniser with CK28 Hard tissue homogenizing 2.0 ml tubes (Bertin) and 1 ml of RLT buffer (Qiagen) supplemented with 1%(v/v ...
-
bioRxiv - Genomics 2021Quote: ... DNA gets separated from proteins and cellular debris using proteinase K (20mg mL-1; Qiagen, Inc., Valencia, CA, USA), precipitated with ethanol and cleaned up ...