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Citations for Qiagen :
2351 - 2400 of 2997 citations for 2 1H Imidazol 1 yl 6 methyl 4 pyrimidinemethanamine since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Synthetic Biology 2024Quote: ... RNA was extracted from 1 ml of the homogenized lysate with the RNeasy Plus Mini kit (Qiagen), including optional on-column DNAse digestion ...
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bioRxiv - Cancer Biology 2024Quote: ... 1 µg of RNA was reverse transcribed into cDNA using the QuantiTect Reverse Transcription Kit (Qiagen, # 205311). Real-time polymerase chain reactions were performed on a Corbett Rotor-Gene 6000 Detection System (Qiagen ...
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bioRxiv - Biophysics 2020Quote: ... reaction mix (for PCR program details see Supplementary Table 1) and purified with QIAquick PCR Purification Kit (QIAGEN). The Fps1-mGFP strain was created by transforming the BY4741 background strain with 100 µL of the purified mGFP-HIS3 fragment with standard LiAc protocol to allow for homologous recombination 34 ...
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bioRxiv - Microbiology 2021Quote: ... This was purified by 1% agarose gel electrophoresis and gel extracted according to QIAquick gel extraction kit (QIAGEN) standard protocol ...
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bioRxiv - Molecular Biology 2021Quote: RNA was isolated from INS-1 and human islets 48 hours post transfection using RNeasy cleanup kit (Qiagen) according to manufacturer’s protocol ...
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bioRxiv - Genomics 2020Quote: Total RNA was purified from eleven samples Table 1 using the QiaAmp viral RNA mini kit (Qiagen – 52906) following viral inactivation at the MRCG at LSHTM containment level 3 facility ...
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bioRxiv - Microbiology 2022Quote: ... beat-beading tubes were filled 1/3 with beads and soaked overnight in 500 μl buffer RLT (Qiagen). Tubes were spun at full speed and excess buffer removed ...
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bioRxiv - Cell Biology 2020Quote: ... 1 μg total RNA was then used for first strand cDNA synthesis using QuantiTect Reverse Transcription Kit (Qiagen). Quantitative real-time PCR was performed using iQ SYBR Green Supermix (Bio-Rad) ...
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bioRxiv - Neuroscience 2020Quote: ... 12,000 GFP+ nuclei were collected into cold Buffer RLT Plus supplemented 1:100 with β-mercaptoethanol (Qiagen, 74034) using the Sony SH800S Cell Sorter (purity mode ...
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bioRxiv - Cancer Biology 2020Quote: ... the first complementary strand was made from 1 µg total RNA using QuantiTect® reverse transcription kit (Qiagen). RT-PCR for the for gene expression analysis was carried out using the CFX-96 thermocycler (Bio-Rad ...
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bioRxiv - Cell Biology 2021Quote: Antibodies and their conditions of use are as follows: mouse anti-His (Western blot, 1:1000; 34660; Qiagen), rabbit anti-Brl1 (Western blot ...
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bioRxiv - Plant Biology 2021Quote: ... PCR products were purified and extracted from a 1% agarose gel using the QIAquick Gel Extraction Kit (QIAGEN), following the manufacturer’s instructions ...
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bioRxiv - Microbiology 2020Quote: Total cellular RNA was extracted from Jurkat cells infected with HIV-1 using the RNeasy Mini Kit (Qiagen) according to manufacturer’s instructions ...
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bioRxiv - Evolutionary Biology 2021Quote: ... was homogenized in 1 ml phosphate-buffered saline (PBS) containing 0.1% Triton X-100 (Thermo) and 0.1% RNase A (Qiagen) using a BioMasher (Nippi ...
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bioRxiv - Plant Biology 2021Quote: ... frozen in liquid nitrogen and ground to fine powder (1 min at 30 Hz in a TissueLyzerII, Qiagen). Total protein was extracted by incubation with extraction buffer (6 µL/leaf disc ...
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bioRxiv - Biochemistry 2020Quote: ... For total RNA isolation a minimum of 1×107 PBMCs were processed using a RNeasy Mini Kit (Qiagen). First-strand synthesis of complimentary DNA from the resulting total RNA was prepared using the SuperScript™ IV First-Strand Synthesis kit (#18091050 ...
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bioRxiv - Microbiology 2020Quote: ... Reverse transcription was performed with 1 µg of the isolated RNA using the QuantiTect Reverse Transcription Kit (Qiagen). mRNA expression levels of ARTD3 ...
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bioRxiv - Microbiology 2020Quote: ... with YFV-C (1-10) K8ac peptide were grown from a solution containing 0.1 M HEPES (pH 7.0) (QIAGEN), 30% PEG 6000 (QIAGEN ...
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bioRxiv - Microbiology 2020Quote: ... with YFV-C (1-10) K4ac/K8ac peptide were grown from a solution containing 0.1 M HEPES (pH 7.0) (QIAGEN), 30% PEG 6000 (QIAGEN ...
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bioRxiv - Bioengineering 2020Quote: ... RT-PCR amplifications were carried out using a Power SYBR Green RNA-to-CT 1 step kit (Qiagen) on a ViiATM Real-Time PCR System (Applied Biosystems ...
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bioRxiv - Evolutionary Biology 2021Quote: We extracted DNA from avian tissue specimens (Supplementary Table 1) using the DNeasy Blood & Tissue Kit (QIAGEN, CA) and generated ddRADtags following the protocol of Peterson et al ...
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bioRxiv - Cancer Biology 2020Quote: ... followed by phenol/chloroform extraction and 1 μg of RNA was reverse transcribed with Quantitect RT kit (Qiagen) following manufacturer’s instructions ...
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bioRxiv - Neuroscience 2022Quote: ... First strand cDNA synthesis from 1 µg of total RNA was performed with QuantiTect Reverse Transcription Kit (Qiagen). Quantitative RT-PCR experiments were performed with Rotor-Gene-Q machine (Qiagen ...
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bioRxiv - Genomics 2022Quote: Total RNA was isolated from cell pellets (<1×107 cells) using the RNeasy Mini kit (QIAGEN, cat. 74134). Cells were lysed with RLT buffer (with no ß-ME ...
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bioRxiv - Developmental Biology 2022Quote: ... miR-1 miRCURY LNA miRNA Power Inhibitor and miRCURY LNA miRNA mimic were obtained from Qiagen (Germantown, MD). miR-1 inhibitor (Hsa-miR-1-3p ...
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bioRxiv - Cancer Biology 2022Quote: RNA was isolated from Myc-CaPWT or Myc-CaPΔRb cells cultured in vehicle or JQ-1 (RNeasy, Qiagen). cDNA was synthesized using the iScript cDNA synthesis kit (BioRad ...
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bioRxiv - Molecular Biology 2020Quote: ... Fragments were extended with homology arms (Table 1) (1000ul NEBnext, Ta = 65, 30 cycles) and purified (Qiagen minelute) to prepare for electroporation ...
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bioRxiv - Cancer Biology 2021Quote: Total RNA from MCF-7 cells (1×106 cells/well) was isolated using RNeasy Mini kit (74106; Qiagen), and 500ng cDNA was synthesized with random hexamers by reverse transcription (SuperScript III ...
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bioRxiv - Microbiology 2021Quote: ... the cell lysate was loaded by gravity flow onto a column containing 1 ml Ni-NTA resin (Qiagen) equilibrated with buffer A ...
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bioRxiv - Neuroscience 2022Quote: ... cDNA was synthesized using 1 µg of RNA per sample according to manufacturer’s instructions (Omniscript RT kit, Qiagen). Taqman assay probes (Thermo Fisher Scientific ...
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bioRxiv - Biochemistry 2022Quote: ... there were 1×106 ESCs culturing in 2iLIF condition for genomic isolation with QIAamp DNA Mini Kit (Qiagen). 500 ng of gDNA was used for bisulfite conversion followed by the instructions of EZ DNA Methylation-Gold Kit (Zymo) ...
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bioRxiv - Cell Biology 2022Quote: ... The clarified lysate was supplemented with Ni-NTA agarose (1.3 ml resin/ 1 l insect cell pellet, Qiagen) and incubated for 30 mins at 4 °C on a rotating wheel ...
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bioRxiv - Plant Biology 2020Quote: ... Roots were washed quickly (< 1 min) to detach the surrounding peat matrix using 10% RNAlater (QIAGEN, Valencia, CA) in 1X PBS as the capture buffer to mitigate RNA degradation ...
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bioRxiv - Cell Biology 2020Quote: ... collected in 1 mM EDTA in PBS and total RNAs were isolated using RNeasy Mini Kit (Qiagen, 74106) according to the manufacturer’s instruction ...
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bioRxiv - Microbiology 2021Quote: ... prior to the addition of proteinase K and 5 μl of 100 mg ml−1 RNase A (Qiagen). The DNA concentration was determined using a Qubit fluorometer (Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2020Quote: ... We then washed beads four times in 1 ml of Modified RLT buffer (RLT buffer supplied by Qiagen with added 10 mM Tris pH 7.5 ...
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bioRxiv - Immunology 2021Quote: Up to 1×106 culture cells were collected in 350 μl of RLT buffer (QIAGEN RNeasy Mini Kit). RNA was extracted following the QIAGEN RNeasy manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2020Quote: ... see Supplemental Table 1 for more details) using either: PN3 ovaries - QIAzol Lysis Reagent (Qiagen, Chadstone, VIC, Australia) and Glycoblue coprecipitant (Thermo Fischer Scientific ...
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bioRxiv - Cell Biology 2020Quote: ... RT-PCR amplifications were carried out using Power SYBR Green RNA-to-CT 1 step kit (Qiagen, UK) on a 384 well ViiA Real-Time PCR System (Applied Bio-systems ...
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bioRxiv - Biochemistry 2021Quote: Total RNAs were extracted from wild-type or DACR HAP-1 with the RNeasy Plus Mini Kit (Qiagen) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2021Quote: Mouse lung tissues were homogenized in 1 ml of MEM-free media using TissueRuptor (Qiagen, Chadstone, Victoria, Australia). The homogenate was obtained by centrifugation at 6,000 × g for 5 min at 4℃ ...
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bioRxiv - Microbiology 2020Quote: Genomic DNA of TIE-1 was isolated using the DNeasy Blood and Tissue kit (Qiagen, Valencia, CA, USA) and used as a template for PCR reactions ...
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bioRxiv - Cell Biology 2021Quote: ... cDNA was prepared from 1 μg of total RNA using the QuantiTect RT Kit (Qiagen, Venlo, the Netherlands). Quantitative RT-PCR was performed using TB Green Premix ExTaq II (Takarabio ...
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bioRxiv - Genomics 2021Quote: ... The reaction was stopped by adding 1 μL 0.5 M EDTA (pH = 8.0) then cleaned up with MinElute PCR Purification Kit (Qiagen) following the manufacturer’s instructions ...
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bioRxiv - Plant Biology 2023Quote: ... All protein sequences (for accession numbers see Supplementary Table 1) were aligned using CLC Genomics Workbench v20.0.4 (Qiagen) and the phylogenetic tree was constructed with the neighbor-joining method using 1000 bootstrap replicates ...
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bioRxiv - Bioengineering 2023Quote: ... 1 μg of RNA per condition was used to retrotranscribe into cDNA QuantiTect® Reverse Transcription Kit (Qiagen). qPCR was performed in 386 wells plates in QuantStudio™ Real-Time PCR System (Applied Biosystems™ ...
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bioRxiv - Genomics 2023Quote: ... 1 μg total RNA was subjected to reverse transcription with a QuantiTect reverse transcription kit (Qiagen, Germantown, MD). The resulting cDNA and Fast SYBR green master mix (Applied Biosystems ...
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bioRxiv - Genomics 2023Quote: ... DNA fragments were run on a 1% agarose gel for imaging or purified on QIAquick columns (28115, Qiagen) for sequencing analysis ...
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bioRxiv - Biochemistry 2023Quote: ... and grown in LB medium (ampicillin 50 μg mL−1) and plasmid purified (miniprep kit Qiagen, Hilden, Germany). Mutations were confirmed by DNA sequencing (Eurofins Genomics ...
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bioRxiv - Immunology 2023Quote: ... An amount of 1 µg of RNA was reverse transcribed using Omniscript Reverse Transcription (RT) Kit (Qiagen, #205113) together with random oligonucleotides (Roche ...