Labshake search
Citations for Qiagen :
201 - 250 of 1551 citations for 3 O tert Butyldimethylsilyl 24 ethyl 24 phenylsulfonyl cholest 5 ene 3 ol d7 since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Plant Biology 2023Quote: ... samples were flash-frozen in liquid nitrogen immediately prior to grinding and ground using 3 mm glass beads at 30 Hz for 3 min in a TissueLyser II (Qiagen) to a fine powder ...
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bioRxiv - Plant Biology 2023Quote: ... Arabidopsis thaliana frozen leaf tissues were weighed and ground by using two 3 mm stainless steel beads for 3 minutes at 30 Hz with frozen adapters on a TissueLyser II (Qiagen). The resulting frozen powder was dissolved in 650 µL chloroform-methanol (3:7 ...
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bioRxiv - Immunology 2024Quote: Total RNA from epididymis white adipose tissue of wide type control mice (n=3) and miR-802 KI mice (n=3) was isolated using the RNeasy mini kit (Qiagen) following the protocol ...
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bioRxiv - Microbiology 2024Quote: ... The DNA of the 20 Jan and 3 Mar 2021 samples (1/3 pellet) was extracted with the DNeasy UltraClean Microbial Kit (Qiagen) following the manufacturer’s instructions ...
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bioRxiv - Genetics 2024Quote: ... Total RNA isolation from Tbx4-CKO (n = 3) and Tbx4fl/fl (n = 3) lungs was performed using the RNeasy Mini Kit (Qiagen). The changes in gene expression were investigated by quantitative reverse transcription polymerase chain reaction (RT-qPCR ...
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bioRxiv - Immunology 2020Quote: ... DNA was extracted at 16-24 hours post infection by the DNA Easy Blood and Tissue Kit (Qiagen) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2020Quote: RNA-Seq libraries were prepared from triplicate 24-hour treated cell lines using the RNeasy extraction kit (Qiagen). Single-end 100bp reads were sequenced with an Illumina HiSeq 2500 ...
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bioRxiv - Genetics 2022Quote: ... Transfected cells were harvested 24 hours after transfection and total RNA was isolated using RNeasy mini kit (Qiagen) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2020Quote: ... each sample tube was tightly closed and loaded onto the PowerLyzer 24 Homogenizer (110/220 V) from Qiagen for bead beating ...
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bioRxiv - Immunology 2020Quote: ... antibodies for 24 hrs before 2000 live cells were FACS sorted directly into lysis buffer (RLT buffer (Qiagen)) ...
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bioRxiv - Cancer Biology 2022Quote: ... for 24 hours at -20°C prior to DNA/RNA extraction with AllPrep DNA/RNA Mini Kit (Qiagen). Cryopreserved patient ascites cells and PDX cancer spheroids were thawed and rinsed in serum free RPMI before RNA/DNA extraction ...
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bioRxiv - Systems Biology 2022Quote: ... siRNA was transfected into RWPE-1 cells in a 24-well plate using HiPerFect Transfection Reagent (Qiagen, 301704). siRNA ...
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bioRxiv - Neuroscience 2023Quote: RNA was extracted from individual wells of 24-well culture plates using a RNeasy Plus Mini Kit (Qiagen) per the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2024Quote: The identified list of 48 ERRα interactors by RIME was analyzed by Metascape v3.5.20230501 [24] and Ingenuity Pathway Analysis (IPA, Qiagen) v 01-22-01 Fall 2023 for enrichment analysis of biological terms and canonical pathways ...
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bioRxiv - Genomics 2023Quote: ... DNA was isolated by phenol:chloroform:isoamylalcohol (25:24:1 pH 8) extraction followed by Qiaquick PCR Purification (QIAGEN, Germany). Purified DNA was then analyzed on a 1.5% agarose gel and on Bioanalyzer (Agilent ...
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bioRxiv - Systems Biology 2024Quote: Fragments were purified by adding 300µL phenol:chloroform:isoamyl alcohol (25:24:1 v/v) and sample to a phase lock tube (Qiagen #129046), and spun down at 16,000g for 3 minutes ...
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bioRxiv - Neuroscience 2024Quote: Total RNAs were extracted from the FACS-isolated EGFP+ OLs with RNeasy Micro kit (Qiagen). For total RNA extraction from the other hemisphere forebrain (without OL sorting) ...
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bioRxiv - Biochemistry 2020Quote: ... 3-4 mL of Ni-NTA resins (Qiagen) were applied on the gravity column ...
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bioRxiv - Microbiology 2022Quote: ... resuspended in 3 ml RNAprotect Bacteria Reagent (Qiagen), divided into 3 × 1 ml aliquots (3 technical replicates) ...
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bioRxiv - Molecular Biology 2021Quote: ... 3) QIAamp DNA Stool Mini Kit (QIAGEN®) and 4 ...
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bioRxiv - Immunology 2021Quote: ... 3-4 pieces were placed into RNAlater (Qiagen) for gene expression analysis ...
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bioRxiv - Microbiology 2024Quote: ... The 3 PCR amplicons were gel purified (Qiagen), added in equimolar quantities ...
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bioRxiv - Genetics 2021Quote: ... and E11 (5’-AGGAAAAAGGAAATAAATTA-3’) primers on pDH373 as a template generated a smaller fragment that was cleaned up by QIAGEN MinElute PCR Purification Kit (#28004) ...
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bioRxiv - Developmental Biology 2021Quote: ... E18.5 RNA was extracted from cortices of 3 genotypic conditional knockouts and 4 controls using the RNeasy Micro Kit (Qiagen). RNA was reverse transcribed to cDNA using the SuperScript™ II Reverse Transcriptase Kit (Invitrogen) ...
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bioRxiv - Cell Biology 2022Quote: ... LRRCC1-si2 (target sequence: 5’- TTA GAT GAC CAA ATT CTA CAA - 3’) and control siRNA (AllStars Negative Control) were purchased from Qiagen. siRNAs were delivered into cells using Lipofectamine RNAiMAX diluted in OptiMEM medium (ThermoFisher Scientific) ...
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bioRxiv - Genomics 2020Quote: ... and template-switching oligo (5′-AAGCAGTGGTATCAACGCAGAGTACrGrG+G-3′, where “r” indicates a ribonucleic acid base and “+” indicates a locked nucleic acid base; Qiagen). cDNA was amplified using KAPA HiFi HotStart ReadyMix kit (Roche #KK2502 ...
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bioRxiv - Microbiology 2020Quote: ... All samples were then passed 3-5 times through a 26G needle prior to RNA isolation using the RNAeasy mini kit from Qiagen. RNA concentrations were estimated by absorbance measurement at 260 and 280 nm ...
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bioRxiv - Molecular Biology 2022Quote: Qiagen miRCURY locked nucleic acid DIG (digoxigenin)-labeled probes (sense cATM-DIG: 5’DIG-AGTGGTTAGACAGTGATGTGT-DIG 3’) (Qiagen, Hilden, Germany) were used for ISH ...
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bioRxiv - Pathology 2021Quote: ... DNA was extracted from epidermal tissue (approximately 3 - 5 mm2 per lesion) using a DNAeasy blood and tissue kit (Qiagen). Regressed and CsA group mice were culled at day 133 post-infection ...
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bioRxiv - Genetics 2021Quote: ... and 99F8 (150 ng pU6-3-99F8-gRNA (DGRC Cat# 1549) and 150 ng pBS-99F8-5’HR-attP>>Act5C::GFP<
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bioRxiv - Cell Biology 2021Quote: Formalin-fixed paraffin-embedded tissues were used for in situ hybridization (ISH) employing locked nucleic acid (LNA) probes labelled with digoxigenin (DIG) at both the 5′- and 3’-ends (Qiagen). ISH was performed with probes specific for miR-132-3p (10 nM ...
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bioRxiv - Developmental Biology 2022Quote: A repair template containing TagRFP-T::AID with homology at the 5’ and 3’ ends to the egl-43 locus was PCR amplified and purified using a PCR purification kit (Qiagen). 3 μl of 10 μM tracRNA (IDT ...
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bioRxiv - Genomics 2022Quote: ... were annealed (10 μL each 100 μM) to 10 μL 5′-[Phos]-CTGTCTCTTATACACATCT-3’ oligonucleotide (100 μM) within 80 μL EB buffer (Qiagen), incubating 2 min at 95°C and cooled to room temperature (0.1°C/sec) ...
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bioRxiv - Plant Biology 2022Quote: ... Total RNA was extracted from 3-5 mm ear primordia of the inbred line B73 using RNeasy Mini Kit (Qiagen) with on-column DNase I (Qiagen ...
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bioRxiv - Genomics 2023Quote: ... Second strand synthesis was performed after thawing by the addition of 5 µL of second strand synthesis mix (3 µL of elution buffer [Qiagen] ...
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Human CCR6+ Th cells show both an extended stable gradient of Th17 activity and imprinted plasticitybioRxiv - Immunology 2023Quote: ... CCR6int and CCR6high subsets of CD4+ T cells were used for isolating RNA without and with prior activation with 20 ng/ml PMA and 1 mM ionomycin for 3 h at 37°C under 5% CO2 using RNeasy Mini Kit (Qiagen) and gene expression levels were determined using Illumina Human WG6 Expression BeadChip Version 3 arrays (Illumina Inc. ...
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bioRxiv - Genomics 2023Quote: ... 1µM Template-Switching Oligo (5′-AAGCAGTGGTATCAACGCAGAGTACrGrG+G-3′, where “r” prefixes a ribonucleic acid base and “+” prefixes a locked nucleic acid base, Qiagen) then incubated using a ThermoMixer C with the following conditions ...
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bioRxiv - Cell Biology 2024Quote: ... SF was transfected with 12.5 nM antisense LNA GapmeRs CBP (Qiagen, Sequence: 5′-GCG GCG ATC CTT TAG A-3′) or p300 (Qiagen ...
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bioRxiv - Cell Biology 2022Quote: Total RNA from patient material (n=3) and organoids (p1 and p2 n=3, p3 n=2) was extracted (RNeasy™ Mini Kit, Qiagen). To obtain cDNA ...
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bioRxiv - Genetics 2021Quote: ... Extraction of genomic DNA was performed for 24 samples in parallel using a Gentra Puregene Yeast/Bact kit (Qiagen). Then ...
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bioRxiv - Physiology 2021Quote: ... and 24 months old mice mesenteric arteries (first branches) by using the RNeasy RNA isolation kit (Qiagen, Germantown, MD) according to the manufacturer’s instruction ...
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bioRxiv - Cell Biology 2022Quote: Cells were seeded at 800,000 in 60 mm plates and 24 h later RNA was isolated (Qiagen RNeasy Kit). 1 ug of RNA was reverse transcribed (Applied Biosystem) ...
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bioRxiv - Cell Biology 2022Quote: Cells were seeded at 800,000 in 60 mm plates and 24 h later RNA was isolated (Qiagen RNeasy Kit). 1 μg of RNA was reverse transcribed (Applied Biosystems™ High-Capacity cDNA Reverse Transcription Kit) ...
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bioRxiv - Microbiology 2020Quote: Cells were harvested 24 h after treatment and RNA was extracted using the RNeasy 96 protocol (Qiagen, Valencia, CA). Gene expression for 22,238 probe sets of 12,490 genes was generated with U133A2.0 microarrays following the manufacturer’s instructions (Affymetrix ...
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bioRxiv - Genomics 2020Quote: Total RNA was harvested at 4 and 24 hours in biological triplicates using the RNeasy plant mini kit (Qiagen). Purified RNA was verified for intactness on a Bioanalyzer 2100 (Agilent ...
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bioRxiv - Immunology 2020Quote: ... were homogenised in a Precellys 24 homogeniser with CK28 Hard tissue homogenizing 2.0 ml tubes (Bertin) and 1 ml of RLT buffer (Qiagen) supplemented with 1%(v/v ...
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bioRxiv - Microbiology 2024Quote: DNA was extracted from 24 model communities (600 μL of each community) using the DNeasy PowerSoil Pro Kit (QIAGEN) according to manufacturers’ instruction ...
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bioRxiv - Genomics 2022Quote: ... Cells were harvested after 24 hours after transfection and total RNA isolated using the RNeasy Maxi kit (Qiagen, 75162) with on-column DNase I digestion ...
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bioRxiv - Immunology 2023Quote: ... Infected cells were incubated for 24 hours prior to genomic DNA extraction using the QIAmp DNA Mini Kit (Qiagen). HIV-1 early and late RT products copy numbers were quantitated by real time PCR as described above ...
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bioRxiv - Immunology 2023Quote: ... 24 hours) treated and untreated control U2OS WT and ZBTB48 KO clones using the RNeasy Plus Mini Kit (Qiagen) with additional on-column DNaseI digestion following the manufacturer’s instructions ...