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Citations for Qiagen :
101 - 150 of 1551 citations for 3 O tert Butyldimethylsilyl 24 ethyl 24 phenylsulfonyl cholest 5 ene 3 ol d7 since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2020Quote: Cells were harvested from 24-well plates in 350μL RLT Plus (Qiagen) supplemented with 1% beta-mercaptoethanol after an 8-hour induction with 10nM E2 or DMSO ...
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bioRxiv - Cell Biology 2020Quote: ... according to the manufacturers’ protocol 24 hours before experiments whereas HiPerfect (Qiagen) reagent was used for transfecting 10 nM siRNA per target 72 hours before experiments ...
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bioRxiv - Plant Biology 2020Quote: ... Initial hits were further optimized in 24-well sitting-drop plates (Qiagen) using as reservoir solution 18-22 % PEG 3350 ...
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bioRxiv - Genomics 2021Quote: ... Sample homogenisation and lysis were performed using PowerLyzer 24 (Qiagen, Hilden, Germany) set at 2,000 rpm for 2 × 5 min ...
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bioRxiv - Cancer Biology 2024Quote: ... the sequencing reads were first imported into CLC Genomics Workbench 24 (Qiagen), merged ...
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bioRxiv - Plant Biology 2023Quote: ... and pXVE::YFP-cPMEI8 plants treated with control MS and 5 µM estradiol for 24 h using the RNeasy Plant Kit (QIAGEN, Hilden, Germany). For RNA isolation from meristematic and elongation zones ...
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bioRxiv - Microbiology 2024Quote: ... Bacteria were then mechanically disrupted using a bead beater homogenizer (PowerLyzer 24, Qiagen; 2 cycles of 45s 5000 rpm / 5 min ice). After an additional 5 min on ice ...
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bioRxiv - Genetics 2021Quote: ... 3×105 U2OS cells were reverse transfected with 5 pmol of each siRNA (QIAGEN) using lipofectamine RNAiMAX (Thermo Fisher ...
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bioRxiv - Bioengineering 2024Quote: ... CHMP6 (Hs_CHMP6_1) target sequence: 5′-CTG AGC GCA ATC ACT CAG GAA -3′ (QIAGEN Sciences LLC ...
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bioRxiv - Neuroscience 2024Quote: ... for 3 minutes with a 5 mm stainless steel ball (Qiagen, Cat. No. 69989). RNA was isolated using the Qiagen RNeasy Lipid Tissue Extraction Kit (Qiagen ...
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bioRxiv - Cancer Biology 2023Quote: ... were transfected with 10 nM Malat1 ENE-targeting (ASO) or control (Con) steric blocking ASOs (Exiqon, Qiagen) using Lipofectamine 2000 (ThermoFisher Scientific) ...
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bioRxiv - Molecular Biology 2020Quote: ... Ligated cDNA samples were cleaned and eluted in 24 μl Elution Buffer (Qiagen) using Oligo Clean & Concentrator (Zymo Research ...
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bioRxiv - Cell Biology 2022Quote: ... was performed approximately 24 h after cell plating using Effectene Transfection Reagent (Qiagen) with 1 µg siRNA in 742 µL transfection mixture ...
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bioRxiv - Physiology 2024Quote: ... The reaction was run in the QIAcuity Nanoplate 26k 24-well plates (QIAGEN). The 40 µl reaction mix consisted of 10 µl of 4x QIAcuity Probe PCR Kit (QIAGEN) ...
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bioRxiv - Immunology 2023Quote: RNA was isolated 24 hours after transfection of HEK293T cells (Qiagen, Cat# 74004). cDNA was synthesized using Verso cDNA synthesis kit (Cat# Ab1453A ...
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bioRxiv - Bioengineering 2023Quote: ... The dPCR reactions were performed in a 26K 24-well Nanoplate (Qiagen, 250001) on a 5-plex QIAcuity One digital PCR instrument (Qiagen ...
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bioRxiv - Microbiology 2024Quote: ... and 24-hours of growth using Qiagen’s RNeasy Mini Spin Kit (Qiagen #74104) and subjected to DNAse digestion by combining 5 µg RNA with 1 µL TURBO DNAse enzyme ...
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bioRxiv - Immunology 2024Quote: ... Total RNA was extracted at 24 hpi using RNeasy Mini kit (Qiagen, #74106) following manufacturer’s specifications ...
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bioRxiv - Physiology 2020Quote: ... Erythrocytes were lysed for 3 minutes with 3 ml of EL buffer (Qiagen). After Fc blocking for 20 minutes ...
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bioRxiv - Cancer Biology 2023Quote: ... 3’mRNA-seq libraries were prepared using QIAseq UPX 3’ Transcriptome Kit (QIAGEN). In brief ...
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bioRxiv - Cell Biology 2023Quote: ... Cleared lysate was briefly incubated (approximately 5 min) with 3 ml Ni-NTA Agarose (QIAGEN) before flow-through was collected ...
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bioRxiv - Bioengineering 2024Quote: ... the 3×Flag-MCS fragments in LV3-SFFV-3×Flag-MCS-GFP (QZ35395, QIAGEN) was replaced by DreAM though NotI and SpeI sites to produce the LV3-SFFV-DreAM-GFP plasmid ...
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bioRxiv - Molecular Biology 2021Quote: ... or 6 µM and harvested 24 h later by adding RLT lysis buffer (Qiagen). Similarly ...
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bioRxiv - Neuroscience 2020Quote: RNA was extracted from 24 hpf sfpq-/- embryos using the RNease Mini Kit (Qiagen). Reverse transcription was performed using the 3’ RACE System for Rapid Amplification of cDNA Ends (ThermoFisher) ...
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bioRxiv - Systems Biology 2021Quote: ... 24 and 48 hpi using the Qiagen RNAeasy plus extraction kit (Qiagen, Hilden, Germany). For quantifying SARS-CoV-2 genome abundance in mock samples ...
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bioRxiv - Evolutionary Biology 2020Quote: ... MinElute purification was carried out using the QIAvac 24 Plus vacuum manifold system (QIAGEN). Parallel non-template controls were included ...
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bioRxiv - Molecular Biology 2021Quote: ... 24 h later cells were transiently transfected with SuperFect Transfection Reagent (Qiagen Ref. 301305), following the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2022Quote: ... gDNA extractions from 24 hpf eggs were performed using QIAamp DNA Mini Kit (Qiagen) following the instructions from the manufacturer ...
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bioRxiv - Cell Biology 2022Quote: ... and 24 hours followed by RNA extraction and purification using the RNeasy kit (Qiagen). Samples were checked for purity and concentration using a nanodrop and Bioanalyser ...
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bioRxiv - Cancer Biology 2022Quote: ... Cells were transfected with hsa-miR-24-3p miRCURY LNA miRNA Power Inhibitor (Qiagen, Germantown ...
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bioRxiv - Microbiology 2020Quote: ... and 24 hours post followed by RNA isolation using an RNeasy mini Kit (Qiagen) per the manufacturer’s recommendations ...
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bioRxiv - Neuroscience 2023Quote: DNAs from 24 hippocampi were purified using the DNeasy Blood and Tissue Kit (Qiagen). Only DNA samples with 260/280 ratios greater than 2.0 were processed for methylation analysis ...
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bioRxiv - Plant Biology 2023Quote: ... The sample was subsequently homogenized for two cycles at 2000 rpm (PowerLyzer 24, Qiagen). The steel beads were removed ...
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bioRxiv - Microbiology 2023Quote: ... and 24 h post treatment and RNA was isolated using RNeasy Mini kit (Qiagen). cDNA was synthesized using the SuperScript III First Strand Synthesis kit (Invitrogen ...
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bioRxiv - Immunology 2024Quote: ... [31] datasets was performed using CLC genomics workbench software version 24 (QIAGEN, https://digitalinsights.qiagen.com) using the Quantify miRNA tool version 1.3 with sequence length set to 15 – 40 nt or 15 – 30 nt ...
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bioRxiv - Molecular Biology 2023Quote: ... An A base was added to the 3’ ends with Klenow (3’-4’ exo-) (Qiagen). The processed ds-cDNA was then ligated to Illumina sequencing adapters with T4 DNA Ligase (Qiagen) ...
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bioRxiv - Biochemistry 2020Quote: ... and pJET_Luc_FL_UTR_REV (5’-GCAATGAAAATAAATGTTTTTTATTAGGCAGAATCCAAATGC-3’) primers and was purified with the QIAquick PCR Purification Kit (Qiagen) directly after the PCR reaction ...
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bioRxiv - Cell Biology 2022Quote: ... A Locked Nucleic Acid (LNA) probe was used to detect centromere regions (5’-TTGGCTACACCATGAAAGCTT-3’; Qiagen). 20 μL of probe mix (250 nM LNA probe ...
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bioRxiv - Evolutionary Biology 2024Quote: ... The obtained 5□- and 3□-RACE products were purified using a QIAquick Gel Extraction Kit (QIAGEN), subcloned into a pTAC-2 Vector (Bio Dynamics Laboratory lnc. ...
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bioRxiv - Microbiology 2024Quote: ... and homogenized by bead-beating with 3 x 5 mm stainless steel beads (Qiagen, Hilden, Germany) in 1 ml sterile 1X PBS to produce internal fly-bacterial suspensions.
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bioRxiv - Microbiology 2024Quote: 3-5 × 105 cells were harvested and total RNA was extracted using the RNeasy kit (Qiagen) employing on-column DNase treatment ...
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bioRxiv - Molecular Biology 2021Quote: ... Unbound Biotin-HPDP was removed by chloroform/isoamylalcohol (24:1) extraction in MaXtract tubes (Qiagen). RNA was precipitated with 10th volume of 5M NaCl and 1 volume of isopropanol ...
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bioRxiv - Neuroscience 2021Quote: DNAs from 24 hemi-hippocampi were purified using the DNeasy Blood and Tissue Kit (Qiagen). Only DNA samples with 260/280 ratios greater than 2.0 were processed for methylation analysis ...
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bioRxiv - Immunology 2022Quote: ... Cells were lysed after 24 hours and RNA was extracted using RNeasy Mini kit (Qiagen) according to manufacturer’s protocol ...
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bioRxiv - Microbiology 2024Quote: ... 24 and 44 hpi and DNA was extracted using DNAeasy blood and tissue kit (Qiagen). Genome copy number quantitation was done by quantitative polymerase chain reaction (qPCR ...
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bioRxiv - Neuroscience 2023Quote: ... ISRIB was withdrawn for 24 hours prior to cell lysis in RT lysis buffer (Qiagen). RNA extraction using the RNeasy Mini Kit (Qiagen ...
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bioRxiv - Microbiology 2023Quote: ... the samples were lysed by bead beating using the Powerlyzer 24 Homogenizer (Qiagen, Germantown, MD) at 2500 rpm for 45 s and then transferred to the QIAcube (Qiagen ...
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bioRxiv - Molecular Biology 2023Quote: ... The PCR reaction mixes were transferred into a 26K 24-well nanoplate (ID: 250001, Qiagen). Partitioning of the PCR mix was performed on the QIAcuity Four digital PCR system after sealing the plate ...
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bioRxiv - Cancer Biology 2023Quote: ... After chloroform: isoamyl alcohol (24:1) extraction followed by purification (Qiagen RNeasy Mini Kit, 74104), the biotinylated RNA was resuspended in water and biotinylated-RNA was isolated using Dynabeads MyOne Streptavidin C1 magnetic beads (Invitrogen) ...
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bioRxiv - Neuroscience 2023Quote: ... The dPCRs were multiplexed in 26K 24-well or 8.5K 96-well QIAcuity nanoplates (QIAGEN) using a QIAcuity system (QIAGEN) ...