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Citations for Qiagen :
2401 - 2450 of 3857 citations for 6 Chloro 4 hydroxy 2 methyl 2H thieno 2 3 e 1 2 thiazine 3 carboxylic acid methyl ester 1 1 dioxide since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2023Quote: ... cells (1 x 106 cells/well) were washed three times with PBS and lysed in Buffer RLT (Qiagen). Lysates were transferred to Eppendorf tubes on ice and stored at -80°C until ready for RNA purification ...
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bioRxiv - Plant Biology 2023Quote: ... All protein sequences (for accession numbers see Supplementary Table 1) were aligned using CLC Genomics Workbench v20.0.4 (Qiagen) and the phylogenetic tree was constructed with the neighbor-joining method using 1000 bootstrap replicates ...
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bioRxiv - Bioengineering 2023Quote: ... 1 μg of RNA per condition was used to retrotranscribe into cDNA QuantiTect® Reverse Transcription Kit (Qiagen). qPCR was performed in 386 wells plates in QuantStudio™ Real-Time PCR System (Applied Biosystems™ ...
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bioRxiv - Genomics 2023Quote: ... 1 μg total RNA was subjected to reverse transcription with a QuantiTect reverse transcription kit (Qiagen, Germantown, MD). The resulting cDNA and Fast SYBR green master mix (Applied Biosystems ...
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bioRxiv - Genomics 2023Quote: ... DNA fragments were run on a 1% agarose gel for imaging or purified on QIAquick columns (28115, Qiagen) for sequencing analysis ...
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bioRxiv - Biochemistry 2023Quote: ... and grown in LB medium (ampicillin 50 μg mL−1) and plasmid purified (miniprep kit Qiagen, Hilden, Germany). Mutations were confirmed by DNA sequencing (Eurofins Genomics ...
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bioRxiv - Immunology 2023Quote: ... An amount of 1 µg of RNA was reverse transcribed using Omniscript Reverse Transcription (RT) Kit (Qiagen, #205113) together with random oligonucleotides (Roche ...
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bioRxiv - Genomics 2023Quote: ... Total RNA was isolated from 1 million cells per condition using the RNeasy Plus Mini Kit (Qiagen, 74104). mRNA was isolated using the Poly(A ...
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bioRxiv - Biochemistry 2023Quote: ... Negatively supercoiled pHOT-1 DNA was purified from DH5α utilizing a Qiagen Plasmid Midi Kit (Qiagen, Hilden, Germany).
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bioRxiv - Cancer Biology 2023Quote: ... Cell pellets were washed with 1× PBS and RNA were extracted using the RNeasy plus mini kit (Qiagen) according to the manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2023Quote: ... RNA was extracted from 1 x 106 transduced HEK293T cells with the RNeasy Plus Mini Kit (Qiagen, #74134) following the manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2023Quote: ... 1 μL of bisulfite treated DNA was amplified for the regions of interest using PyroMark PCR kit (Qiagen) with final primer concentration of 0.4 μM ...
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bioRxiv - Genomics 2023Quote: ... DNA was isolated by phenol:chloroform:isoamylalcohol (25:24:1 pH 8) extraction followed by Qiaquick PCR Purification (QIAGEN, Germany). Purified DNA was then analyzed on a 1.5% agarose gel and on Bioanalyzer (Agilent ...
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bioRxiv - Cancer Biology 2023Quote: Approximately 1 cm of the mouse distal ileum was cut into small pieces and stored in RNAlater (Qiagen). RNA was prepared using a combination of Trizol (Ambion ...
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bioRxiv - Microbiology 2023Quote: ... Cells were then disrupted by sonication and the supernatant was mixed with 1 mL Ni-NTA agarose (QIAGEN) and incubated for 1 h at 4 °C with gentle shaking ...
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bioRxiv - Immunology 2023Quote: ... Total DNA was obtained from the lysed inoculated THP-1 macrophages using DNeasy Blood & Tissue Kit (Qiagen, Germany). The amplification reaction was performed in a 20μl final volume containing 1μl of template DNA ...
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bioRxiv - Microbiology 2023Quote: ... 1 mL of culture was withdrawn and chromosomal DNA extracted using the Dneasy blood and tissue kit (Qiagen). The samples were normalized according to DNA concentrations and diluted 1:5 ...
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bioRxiv - Evolutionary Biology 2023Quote: DNA extraction was carried out using one of the following methods: 1) QIAmp DNA Mini Kit (Qiagen, Germany); 2 ...
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bioRxiv - Systems Biology 2023Quote: ... Amplicons were gel-purifed from a 1 % Agarose gel using the QIAquick Gel Extraction Kit (QIAGEN, Düren, Germany). After determining the concentration with Qubit (Invitrogen ...
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bioRxiv - Evolutionary Biology 2023Quote: ... Genomic DNA was isolated from 1 mL of overnight culture of each genotype (DNeasy Blood & Tissue Kit; Qiagen). Paired-end ...
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bioRxiv - Cancer Biology 2023Quote: ... Up to 1 ug of RNA was converted into cDNA using either the Quantitect Reverse Transcription kit (QIAGEN) or the Superscript IV transcription kit (ThermoFisher Scientific ...
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bioRxiv - Microbiology 2023Quote: ... All treatment groups were supplemented with 1 % FCS and RNA was extracted in RNAeasy Plus lysis buffer (QIAGEN) 6 h post treatment.
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bioRxiv - Genomics 2023Quote: ... V6.5 mESCs and K562 genomic DNA was purified from 1 million cells using the QIAamp micro kit (QIAGEN), according to the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2023Quote: ... The supernatant was loaded using a peristaltic pump in 1 mL (bed volume) of Ni-NTA agarose (QIAGEN) packed in a Poly-Prep® Chromatography Column (Cat #731-1550 ...
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bioRxiv - Systems Biology 2024Quote: Fragments were purified by adding 300µL phenol:chloroform:isoamyl alcohol (25:24:1 v/v) and sample to a phase lock tube (Qiagen #129046), and spun down at 16,000g for 3 minutes ...
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bioRxiv - Cell Biology 2024Quote: ... or Disp-/- cells were seeded into six-well plates and transfected with 1 µg Shh constructs together with 0.5 µg Scube2 or empty cDNA3.1 by using Polyfect (Qiagen). Where indicated ...
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bioRxiv - Cell Biology 2024Quote: Total RNA was extracted from 1 x 108 trypanosome cells using the Qiagen RNeasy Mini Kit (Qiagen, Netherlands) following the manufacturer’s instructions ...
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bioRxiv - Immunology 2024Quote: ... Tissues were then resuspended in 1 mL of lysis buffer from the RNeasy Fibrous Tissue Mini Kit (Qiagen) and processed as per the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2024Quote: ... 1 µg of the isolated total RNA was transcribed using the QuantiTect Reverse Transcription Kit (Qiagen, Hilden, Germany). The quantitative real-time PCR (qRT-PCR ...
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bioRxiv - Cancer Biology 2021Quote: ... RNA of PDOs grown in ‘Type 1’ culture medium was isolated according to the manufacturer’s protocol using the RNeasy Mini Kit (QIAGEN). Quality and quantity of the RNA samples and the libraries were measured with Agilent’s Bioanalyzer2100 and Invitrogen™ Qubit™ 3.0 Fluorometer ...
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bioRxiv - Cell Biology 2019Quote: ... Total RNA was isolated following the manufacturer’s protocol and cDNA was synthesized from 1 μg RNA using the QuantiTekt Reverse Transcription kit (Qiagen) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2020Quote: ... Cells were washed once with 1 % BSA and incubated with a solution containing 200 μg/ml RNase A (Qiagen) and 50 μg/ml Propodium Iodide (Sigma ...
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bioRxiv - Microbiology 2019Quote: ... Cells were washed with PBS and incubated at 37°C with a 1:100 dilution of RNase A (QIAGEN) in PBS for 3 hours ...
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bioRxiv - Evolutionary Biology 2020Quote: ... 1 μL of primer mix (with a fluorescently labelled forward primer) at 0.2 μM and 1 μL QIAGEN Multiplex PCR Master Mix (QIAGEN). PCR cycling conditions were 95 °C for 10 min followed by 35 cycles of 95 °C for 30 s ...
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bioRxiv - Pathology 2022Quote: ... RNA was isolated from maternal and fetal tissues (Table 1) using QIAamp cador pathogen mini kit (Qiagen, Valencia, CA). ZIKV RNA was quantitated by one-step quantitative real time reverse transcription PCR using QuantiTect probe RT-PCR kit (Qiagen ...
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bioRxiv - Physiology 2022Quote: ... The tissue was homogenized for 1 min at 28.5 Hz in a TissueLyser (Qiagen TissueLyser II, Retsch GmbH, Germany) before samples were rotated end over end for 1 hour at 4°C and centrifuged at 18320g for 20 min at 4°C ...
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bioRxiv - Microbiology 2022Quote: ... Pellets were then resuspended in 500 μL of supernatant and stabilized with 1 mL of RNAprotect Bacteria Reagent (Qiagen). The RNeasy® Protect Bacteria Mini Kit (Qiagen ...
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bioRxiv - Molecular Biology 2021Quote: HIV-1 viral RNA was extracted from 20-50 ul of plasma using QIAamp Viral RNA mini kit (Qiagen). qRT-PCR was performed using a TaqMan Fast Virus 1-Step Master Mix ...
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bioRxiv - Genomics 2020Quote: ... the final clean-up was performed at 0.9:1 ratio of beads to PCR products and elution again done in 16μl EB (Qiagen, Germany). Samples were diluted to 8pM final concentration before sequencing on an Illumina Miseq (2×25nt).
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bioRxiv - Synthetic Biology 2019Quote: ... The PCR products were separated on a 1% agarose gel and purified using a QIAquick Gel Extraction Kit (Qiagen). The parts were assembled using the NEB HiFi DNA Assembly kit (New England Biolabs ...
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bioRxiv - Developmental Biology 2019Quote: ... each collection lid was covered with 15μl of a 1:16 dilution of proteinase K in PKD buffer (Qiagen), incubated at room temperature for 5 min and subsequently snap frozen in dry ice and stored at −80°C overnight ...
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bioRxiv - Pathology 2020Quote: Total RNA was extracted from the BALF sample of patient 1 using the RNeasy Plus Universal Mini Kit (Qiagen) following the manufacturer’s instructions ...
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bioRxiv - Genomics 2019Quote: ... 750ng of the gDNA was labeled using DLE-1 enzyme and reaction mix followed by Proteinase K digestion (Qiagen). The DNA back bone was stained after drop dialysis ...
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bioRxiv - Plant Biology 2019Quote: ... First-strand cDNA was synthesized from 1 μg of total RNA using QuantiTect® Reverse Transcription kit (Qiagen, Germany).
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bioRxiv - Molecular Biology 2021Quote: HOS cells were cotransfected with a Rev/Env-encoding pSVIIIenv plasmid and a Tat-encoding plasmid at a 1:0.125 ratio using the Effectene transfection reagent (Qiagen). At 48 h after transfection ...
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bioRxiv - Neuroscience 2020Quote: ... qPCR was performed on cDNA dilutions of 1/20 in the presence of 7.5-pmol primers with QuantiTect SYBR Green PCR Master Mix (Qiagen) on a Lightcycler 480 (Roche) ...
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bioRxiv - Microbiology 2021Quote: Virus isolation was performed on tissues following homogenization in 1 mL DMEM using a TissueLyser (Qiagen, Germantown, MD, USA) and inoculating Vero E6 cells in a 96 well plate with 200 µL of 1:10 serial dilutions of the homogenate ...
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bioRxiv - Neuroscience 2021Quote: ... Reverse transcription was performed according to the manufacturer’s recommendations on 1 μg of RNA with the QuantiTect Reverse Transcription Kit (Qiagen), which includes a genomic DNA–removal step ...
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bioRxiv - Microbiology 2020Quote: ... A total of OD600 = 0.3 in 1 ml of cells was pelleted and gDNA was extracted using DNeasy Blood & Tissue kit (Qiagen) using the Gram-negative bacterial protocol followed by quantification using Qubit ...
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bioRxiv - Plant Biology 2021Quote: ... ABI1 and ABI2 with primers listed in Supplemental Table 1 using the Rotor Gene SYBR Green PCR Kit (Qiagen) according to manufacturers’ protocol in a Rotor gene Q cycler (Qiagen) ...