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Citations for Qiagen :
2301 - 2350 of 3857 citations for 6 Chloro 4 hydroxy 2 methyl 2H thieno 2 3 e 1 2 thiazine 3 carboxylic acid methyl ester 1 1 dioxide since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genomics 2022Quote: ... DNA from I-73-1 and D308 was extracted using a ‘Genomic-tip 100/G’ kit (Qiagen) and long-reads sequenced with PacBio RS II at 100x coverage ...
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bioRxiv - Genetics 2022Quote: ... or approximately 30 mg tissue material homogenized in 1 ml TRIzol™ reagent via TissueLyser II (Qiagen) with a 5 mm steel bead for 2 min at 30 Hz ...
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bioRxiv - Microbiology 2022Quote: ... Ligation of 1 µl 5′RACE PCR products were performed with the Qiagen PCR cloning kit (Qiagen) according to the manufacters protocol ...
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bioRxiv - Immunology 2022Quote: cDNA was generated from 1 µg RNA with the Omniscript® Reverse Transcription Kit (Qiagen, Hilde, Germany) using oligoDT12-18 primer (Thermo Fisher Scientific Inc. ...
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bioRxiv - Microbiology 2024Quote: ... and bead-beating them three times for 1 minute at 30 Hz using a TissueLyser II (Qiagen) and the steel beads included in the DNeasy Plant Pro Kit (Qiagen ...
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bioRxiv - Microbiology 2023Quote: ... 5 ml of the supernatant were added to 1 ml of Nickel-NTA Agarose (Qiagen, Hilden, Germany) and incubated under continuous shaking at 4 °C for 1 h ...
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bioRxiv - Microbiology 2024Quote: ... total DNA from 1×106 cells was extracted using the Blood & Cell Culture DNA Mini Kit (Qiagen). Extracted DNA was diluted to 10 ng/μl and subjected to qPCR analysis ...
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bioRxiv - Neuroscience 2024Quote: ... DNA was extracted from 1-5E5 sorted cells using the DNeasy blood and tissue kit (Qiagen, 69504) and used for barcode amplification and NGS analysis.
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bioRxiv - Molecular Biology 2023Quote: Cell-free RNAs (cfRNAs) were extracted from 1 ml of plasma using QIAzol Lysis Reagent (Qiagen, 79306) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2023Quote: ... 1 µg of RNA was used per sample using the QuantiTect Reverse Transcription Kit (Cat# 205311, Qiagen). The quantitative RT-qPCR reactions were carried out using the PowerUp™ SYBR™ Green Master Mix (Cat# A25742 ...
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bioRxiv - Molecular Biology 2022Quote: ... for 1 min 30 sec at 30 Hz before RNA extraction using the RNeasy micro kit (Qiagen). RNA was sequenced using the DNBseq platform at the Beijing Genomics Institute (BGI Group ...
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bioRxiv - Microbiology 2023Quote: ... The purified recombinant proteins were analyzed by western-blot with anti-His antibody (1:2000 dilution) (Qiagen) followed by HRP-labeled anti-mouse IgG (1:50000 dilution ...
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bioRxiv - Immunology 2023Quote: ... 1×105 cells were removed four days after electroporation and DNA isolated using the DNeasy kit (Qiagen) according to manufacturer’s instructions ...
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bioRxiv - Genomics 2023Quote: ... Reverse transcription was performed from 1 µg RNA using the Quantitect Reverse Transcription Kit (QIAGEN, Manchester, UK) and cDNA was diluted to 10 ng/µl equivalent before use in qPCR ...
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bioRxiv - Physiology 2023Quote: ... A small portion of frozen livers (∼20 mg) were homogenized in 1 mL of QIAzol (Qiagen 79306). After the addition of 200 µL of chloroform ...
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bioRxiv - Synthetic Biology 2023Quote: ... Each PCR reaction was extracted from a 1% agarose gel and column purified using QG buffer (Qiagen), following manufacture instructions ...
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bioRxiv - Cancer Biology 2023Quote: Genomic DNA was prepared from cell pellets (1 million cells) using Allprep DNA/RNA mini kit (Qiagen). Whole genome libraries were sequenced 150x2 bp on a NovaSeq 6000 (Illumina ...
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bioRxiv - Developmental Biology 2023Quote: ... cDNA synthesis was carried out with 1 µg of RNA using the QuantiTect Reverse Transcription Kit (Qiagen) and subsequently diluted 1:25 in dH2O ...
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bioRxiv - Microbiology 2023Quote: Genomic DNA (gDNA) was extracted from 1×106 PBMCs using the QIAamp DNA Blood Mini Kit (Qiagen) per manufacturers’ instructions ...
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bioRxiv - Molecular Biology 2023Quote: ... cDNA was prepared from 1 μg of total RNA using a QuantiTect™ Reverse Transcription kit (Qiagen) from random hexamer primers ...
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bioRxiv - Cell Biology 2023Quote: ... Aliquots of 1 μg of RNA were reverse transcribed using a QuantiTect Reverse Transcription kit (Qiagen, 205314), and qPCR was performed with iTaq SYBR Green (Life Technologies ...
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bioRxiv - Developmental Biology 2024Quote: ... PCR products were confirmed with a 1% agarose gel and PCR purified (QIAquick PCR Purification kit, QIAGEN). The dsRNA was synthesized using a T7 reverse transcription reaction kit (MEGAscript ...
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bioRxiv - Microbiology 2024Quote: ... His-tagged proteins were detected with a mouse anti-His HRP-conjugated antibody (1:3000) from Qiagen. MBP fusion proteins were detected with a rabbit anti-MBP from NEB company 1:1000 followed by an incubation with a goat anti-rabbit HRP-conjugated (1:3000) ...
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bioRxiv - Genetics 2024Quote: DNA was isolated using 1 ml from whole blood using the QiAamp DNA blood Midi kit (Qiagen) following the manufacturer’s instructions ...
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bioRxiv - Genetics 2024Quote: ... The entire HTT exon 1 coding sequence was amplified using UltraRun® LongRange PCR Kit (QIAGEN, 206442) with 10% DMSO using the following PCR conditions ...
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bioRxiv - Microbiology 2023Quote: ... The SCGs cultured in 6-well dishes and one entire 6-well was harvested and pooled together for RNA isolation (RNeasy, Qiagen) per sample ...
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bioRxiv - Neuroscience 2024Quote: ... 6 µl Hi-Perfect transfection reagent (Qiagen, 301707), and 1 µl of the desired (20 µM ...
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bioRxiv - Microbiology 2022Quote: Fecal samples positive for the partial E gene of sarbecovirus were homogenized in TissueLyser II (Qiagen) using 0.1 mm glass beads (Tomy Seiko ...
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bioRxiv - Biochemistry 2020Quote: ... Locked nucleic acid (LNA) ASOs were ordered from Qiagen and dissolved in water to 100 μM ...
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bioRxiv - Biochemistry 2019Quote: ... mixed with nickel-nitrilotriacetic acid-agarose resin (NiNTA, Qiagen) and allowed to rotate end-over- end at 4°C for 4 hours ...
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bioRxiv - Genomics 2021Quote: ... Using the QIAmp Circulating Nucleic Acid Kit (Qiagen, 55114) we followed two of the manufacturer protocol ...
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bioRxiv - Cell Biology 2020Quote: ... Nucleic acids were extracted using the RNeasy kit (QIAGEN). Contaminating DNA was removed using the Turbo DNA-free kit (Thermo Fisher Scientific) ...
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bioRxiv - Cell Biology 2023Quote: The antisense locked nucleic acid (LNA) GapmeR (#339515, Qiagen) sequence for the Tug1 LNA was (5’-3’ ...
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bioRxiv - Microbiology 2023Quote: ... nucleic acids were extracted (QIAmp DNA Mini Kit, QIAGEN) and a multiplex nested PCR was implemented to detect known skin-tropic HPyVs (MCPyV ...
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bioRxiv - Genetics 2023Quote: ... nucleic acid was purified using QiaQuick (QIAGEN, Germantown, Maryland) and sent to Laragen for Sanger sequencing (Laragen ...
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bioRxiv - Biophysics 2020Quote: ... reaction mix (for PCR program details see Supplementary Table 1) and purified with QIAquick PCR Purification Kit (QIAGEN). The Fps1-mGFP strain was created by transforming the BY4741 background strain with 100 µL of the purified mGFP-HIS3 fragment with standard LiAc protocol to allow for homologous recombination 34 ...
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bioRxiv - Microbiology 2021Quote: ... This was purified by 1% agarose gel electrophoresis and gel extracted according to QIAquick gel extraction kit (QIAGEN) standard protocol ...
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bioRxiv - Molecular Biology 2021Quote: RNA was isolated from INS-1 and human islets 48 hours post transfection using RNeasy cleanup kit (Qiagen) according to manufacturer’s protocol ...
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bioRxiv - Genomics 2020Quote: Total RNA was purified from eleven samples Table 1 using the QiaAmp viral RNA mini kit (Qiagen – 52906) following viral inactivation at the MRCG at LSHTM containment level 3 facility ...
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bioRxiv - Genomics 2019Quote: Total DNA was extracted from 1 ml of blood sample using DNeasy Blood and Tissue Kit (Qiagen, USA). The concentration of DNA ranged from 2.2 ng/ul to 9.7 ng/ul ...
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bioRxiv - Molecular Biology 2019Quote: ... The peak fractions containing Esco2 were pooled and applied onto a 1 ml Ni-NTA Superflow column (Qiagen), equilibrated with 20 mM HEPES pH 7.2 ...
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bioRxiv - Molecular Biology 2019Quote: ... The PCR products were purified from a 1% agarose gel using the QIAquick gel extraction kit (Qiagen, Inc.) and cloned in NdeI and XhoI sites of pET-22b (+ ...
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bioRxiv - Cell Biology 2019Quote: ... 1 ml of culture was harvested for RNA extraction using an RNeasy® Mini Kit (Qiagen, Hilden, Germany). DNA digestion was done to remove contaminating DNA in the solution before RNA clean up ...
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bioRxiv - Cell Biology 2020Quote: ... 1 μg total RNA was then used for first strand cDNA synthesis using QuantiTect Reverse Transcription Kit (Qiagen). Quantitative real-time PCR was performed using iQ SYBR Green Supermix (Bio-Rad) ...
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bioRxiv - Neuroscience 2020Quote: ... 12,000 GFP+ nuclei were collected into cold Buffer RLT Plus supplemented 1:100 with β-mercaptoethanol (Qiagen, 74034) using the Sony SH800S Cell Sorter (purity mode ...
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bioRxiv - Cancer Biology 2020Quote: ... the first complementary strand was made from 1 µg total RNA using QuantiTect® reverse transcription kit (Qiagen). RT-PCR for the for gene expression analysis was carried out using the CFX-96 thermocycler (Bio-Rad ...
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bioRxiv - Cell Biology 2021Quote: Antibodies and their conditions of use are as follows: mouse anti-His (Western blot, 1:1000; 34660; Qiagen), rabbit anti-Brl1 (Western blot ...
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bioRxiv - Plant Biology 2021Quote: ... PCR products were purified and extracted from a 1% agarose gel using the QIAquick Gel Extraction Kit (QIAGEN), following the manufacturer’s instructions ...
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bioRxiv - Microbiology 2020Quote: Total cellular RNA was extracted from Jurkat cells infected with HIV-1 using the RNeasy Mini Kit (Qiagen) according to manufacturer’s instructions ...
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bioRxiv - Evolutionary Biology 2021Quote: ... was homogenized in 1 ml phosphate-buffered saline (PBS) containing 0.1% Triton X-100 (Thermo) and 0.1% RNase A (Qiagen) using a BioMasher (Nippi ...