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Citations for Qiagen :
2251 - 2300 of 10000+ citations for C Reactive Protein CRP ELISA Kit 5 Plate since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biophysics 2020Quote: ... Debris was removed by centrifugation (20,000×g, 30 min, 4°C) and the supernatant was incubated with Ni-NTA resin (Qiagen) for 30 min at 4°C ...
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bioRxiv - Molecular Biology 2020Quote: ... was tagmented with Tn5 transposase (25 μM) for 30 min at 55°C and purified using MinElute columns (QIAGEN).
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bioRxiv - Biochemistry 2020Quote: ... The lysates were cleared for 30 min at 45,000 × g at 4°C and incubated with 1 ml of Ni-NTA agarose beads (Qiagen) per 1 l of expression culture for 2 h rotating at 4°C ...
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bioRxiv - Biochemistry 2021Quote: ... Lysates were clarified by centrifugation for 1 h at 4°C at 10,000 rcf before being subjected to Ni-NTA (Qiagen) affinity purification following the manufacturer’s protocol.
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bioRxiv - Molecular Biology 2021Quote: ... The solution was further cleared at 20,000 × g at 4 °C and the supernatant was incubated with Ni-NTA resin (Qiagen) for 2 h at 4 °C ...
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bioRxiv - Cell Biology 2021Quote: ... Proteins were expressed in BL21de3 E.coli by auto induction in LB medium at 18°C and purified using Ni-NTA beads (Qiagen). When present ...
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bioRxiv - Immunology 2022Quote: ... Lysate was cleared from debris by centrifugation at 30,000 g and 4°C for 30 min before being loaded onto a Ni-NTA Superflow cartridge (Qiagen) using an Äkta Explorer chromatography system (GE Healthcare) ...
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bioRxiv - Cancer Biology 2024Quote: ... Cell lysates were further transferred to liquid N2/ -80 °C freezer before RNA purification according to Qiagen protocol (www.Qiagen.com ...
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bioRxiv - Genomics 2024Quote: ... 72 °C for 1 min and then subjected to a 1.2x SPRI cleanup eluting in 42 µl EB buffer (Qiagen). Each sample was split into two fractions and each of them was further amplified with modality-specific primers ...
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bioRxiv - Microbiology 2024Quote: ... Lysates were clarified by centrifugation at 21,000× g at 4°C and loaded onto gravity flow Ni-NTA agarose columns (Qiagen), followed by washing with 50 mM HEPES pH 7.5 ...
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bioRxiv - Biochemistry 2023Quote: ... The lysate was cleared by centrifugation at 10,000g for 30 min at 4 °C and applied to 15-mL equilibrated Ni-NTA beads (Qiagen). The Ni-NTA column was washed with 150 mL of buffer B (20 mM Tris-HCl pH 8.0 ...
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bioRxiv - Biochemistry 2023Quote: ... The lysate was cleared by centrifugation at 10,000g for 30 min at 4 °C and applied to 15-mL equilibrated Ni-NTA beads (Qiagen). The Ni-NTA column was washed with 150 mL of buffer B (20 mM Tris-HCl pH 8.0 ...
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bioRxiv - Neuroscience 2023Quote: ... This was followed by three washes with 2X SSC at 37°C and treatment with RNaseA (200ng/ml; Qiagen) in 2X SSC at 37°C ...
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bioRxiv - Microbiology 2023Quote: ... Cell lysate was cleared by centrifugation at 100,000 × g for 30 min at 4 ⁰C and then incubated with Ni2+-NTA agarose beads (Qiagen) for 30 min at 4⁰C ...
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bioRxiv - Cell Biology 2022Quote: ... The tagmentation reaction mixture was transferred in a 1.5ml low-binding tube and incubated at 37°C for 30 min and stopped by the addition of 250μl Buffer PB (Qiagen). The tagmented chromatin was purified using the MinElute PCR purification kit (Qiagen 28004) ...
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Altered motility in response to iron-limitation is regulated by lpdA in uropathogenic E. coli CFT073bioRxiv - Microbiology 2023Quote: ... Strains were cultured for five hours at 37°C with aeration before harvest and treatment with bacterial RNAprotect (Qiagen). Bacterial pellets were stored at -80°C until RNA isolation.
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bioRxiv - Microbiology 2023Quote: ... The soluble lysates obtained after two rounds of centrifugation at 18,000g and 4°C were incubated with glutathione agarose beads (Pierce) or Ni2+-NTA beads (QIAGEN). Recombinant proteins were eluted from the beads using 10 mM reduced glutathione in a Tris (50 mM Tris-HCl ...
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bioRxiv - Biochemistry 2024Quote: ... The culture was then combined with LB + 25 µg/mL chloramphenicol + 0.2% glucose (w/v) culture for overnight growth (37 °C) and then miniprepped (Qiagen).
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bioRxiv - Microbiology 2024Quote: ... The lysate was cleared by centrifugation at 10,000g for 30 min at 4 °C and applied to 15-mL equilibrated Ni-NTA beads (Qiagen). The Ni-NTA column was washed with 150 mL of buffer B (20 mM Tris-HCl pH 8.0 ...
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bioRxiv - Microbiology 2024Quote: ... The lysate was cleared by centrifugation at 10,000g for 30 min at 4 °C and applied to 15-mL equilibrated Ni-NTA beads (Qiagen). The Ni-NTA column was washed with 150 mL of buffer B (20 mM Tris-HCl pH 8.0 ...
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bioRxiv - Molecular Biology 2024Quote: ... The soluble lysate obtained after centrifugation at 13,000 rpm for 30 minutes at 4°C was incubated with Ni-NTA agarose resin (Qiagen) for 1 hour at 4°C ...
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bioRxiv - Microbiology 2024Quote: ... DNA on the exterior of filtered capsids was digested for 1 h at 25°C with 20.3 units DNase (79254; Qiagen) supplemented with 10 mM MgCl2 ...
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bioRxiv - Molecular Biology 2022Quote: ... 10,000 cells per sample were sorted into a 96 well plate containing 50 μl RLT plus Lysis Buffer (Qiagen) supplemented with 1% 2-mercaptoethanol and frozen at −80 °C immediately ...
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bioRxiv - Molecular Biology 2019Quote: Cells seeded in 24-well plates were transfected with siRNA against intended targets (Qiagen, sequences provided in Table S3) using Lipofectamine RNAiMAX (Thermo Fisher ...
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bioRxiv - Cell Biology 2019Quote: ... 1.2×106 S2R+ cells were seeded onto each well of a 6-well plate and transfected using Effectene (Qiagen) with either pAc-Eff1-HA (150 ng) ...
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bioRxiv - Systems Biology 2020Quote: ... Samples were then eluted into a clean 96DW plate from each well using 110 μL of Buffer AE (QIAGEN) preheated to 56°C ...
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bioRxiv - Systems Biology 2020Quote: ... The sealed plates were subjected to 10 minutes of bead beating at 20 Hz using an (Qiagen Tissuelyser II). After bead beating ...
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bioRxiv - Developmental Biology 2022Quote: ... Cells were plated in 12 well plates at a dilution of 1.5×106 and were transfected with the indicated constructs using Effectene (Qiagen) according to manufacturer’s protocols ...
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bioRxiv - Molecular Biology 2022Quote: 2.0 × 105 HeLa cells were transfected in 12 well plates with control (NTC: all-stars non-targeting control, Qiagen) or DDX39B-targeting (D11 ...
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Deconstructing the role of iron and heme in the structure of healthy human gut microbial communitiesbioRxiv - Microbiology 2022Quote: ... Two hundred fifty microliters of each sample were transferred into 96-well PowerBead Plates (Qiagen, 27500-4-EP-BP). Seven hundred fifty microliters of RLT buffer from an AllPrep DNA/RNA 96 Kit (Qiagen ...
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bioRxiv - Plant Biology 2022Quote: ... Sections were immediately placed into 96-well plates (on ice) containing 10 μL of RLT plus (Qiagen, Hilden, Germany). All instruments and surfaces were cleaned with 80% ethanol ...
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bioRxiv - Neuroscience 2021Quote: Total RNA was isolated from hESCs or NPCs grown in a 6-well plate (9.5 cm2) using the RNeasy minikit (Qiagen). A DNase 1 digestion step was performed during RNA extraction to degrade remaining genomic DNA ...
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bioRxiv - Immunology 2022Quote: ... Single cells from three to four mice per each condition were index-sorted directly into 96-well plates containing 5μl of TCL buffer (Qiagen) supplemented with 1%β-mercaptoethanol using a BD FACS Aria II instrument ...
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bioRxiv - Molecular Biology 2019Quote: 1×106 MIA PaCa-2 or HAP1 cells were seeded per 6 well plate and co-transfected with 0.5 µg pCas-Guide plasmid using Effectene transfection reagent (Qiagen). 24 hrs after transfection ...
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bioRxiv - Cell Biology 2022Quote: ... and RT2 Profiler PCR Array Mouse WNT Signaling Pathway ABI 7900HT Standard Block plates (Qiagen; Cat. No. PAMM-043ZA) were used for qPCR.
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bioRxiv - Molecular Biology 2023Quote: ... 10µL of diluted nuclei were distributed to all wells of a 96-well plate and 1µL EB buffer (Qiagen) 0.5µL Proteinase K (Qiagen) ...
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bioRxiv - Molecular Biology 2023Quote: ... and sorted into wells of a 96-well plate (185 nuclei/well) containing reverse cross-linking buffer (11µL EB buffer (Qiagen) 0.5µL Proteinase K (Roche) ...
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bioRxiv - Immunology 2022Quote: His-tagged RBD of SARS-CoV S was diluted to a concentration of 0.8 μg/mL in Tris-buffered saline (TBS) and immobilized on NiNTA 96-well plates (Qiagen) for 2h at RT ...
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bioRxiv - Synthetic Biology 2024Quote: ... Samples were prepared in duplicates in a standard 96-well PCR plate and transferred to a QIAcuity 8.5k 96-well Nanoplate (Qiagen) for partitioning using the Qiagen Standard Priming Profile ...
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bioRxiv - Molecular Biology 2024Quote: ... Cells were seeded in 24-well plates and transfected with the desired plasmids using Effectene Transfection Reagent (#301427, QIAGEN) following the manufacturer’s protocol ...
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bioRxiv - Immunology 2024Quote: ... or PMs were washed with PBS and then lysed in a culture plate using QIAzol Lysis Reagent (Qiagen 79306) as was described in our previous studies ...
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bioRxiv - Microbiology 2023Quote: ... Bacterial biomass from strains grown on SKV agar plates was resuspended directly in 300 μl of PowerBead Solution (Qiagen) and vortexed to mix ...
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bioRxiv - Immunology 2023Quote: ... 100 μl His-tagged trimer diluted to 1.5 μg/ml in PBS (pH 7.4) or PBS only as a control was added per well to Ni- NTA 96-well plates (Qiagen) and incubated over night at 4°C ...
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bioRxiv - Evolutionary Biology 2023Quote: ... Bacterial biomass from isolates grown on SKV agar plates was resuspended directly in 300 μl of PowerBead Solution (Qiagen) and vortexed to mix ...
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bioRxiv - Developmental Biology 2023Quote: Plates with the micropatterned coverslips were washed with PBS-/- and the cell colonies lysed in RLT buffer (QIAGEN 1015762). RNA extraction was performed using RNeasy mini kit (QIAGEN 74106 ...
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bioRxiv - Immunology 2023Quote: M1 and M2 macrophages (300,000/well, 96-well plate) were transfected on Day 7 with MALAT1 or control GapmeRs (Qiagen). MALAT1- or control GapmeR-transfected M1 and M2 Mφ were incubated with Texas Red-conjugated Ova and DQ-conjugated Ova (both 1 mg/ml ...
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bioRxiv - Systems Biology 2023Quote: ... Samples were then eluted into a clean 96DW plate from each well using 110 μL of buffer AE (QIAGEN) preheated to 56 °C ...
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bioRxiv - Genomics 2020Quote: ... PCR products were gel-purified with a DNA gel extraction kit (QIAquick Gel Extraction Kit, Qiagen) and used for library preparation with TruSeq/ChIP-Seq reagents (Illumina) ...
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bioRxiv - Developmental Biology 2021Quote: ... Total RNA isolation was then carried out using a RNA extraction Kit (RNeasy Mini Kit, Qiagen). scRNA-seq was performed using the Smart-seq2 protocol68 ...
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bioRxiv - Molecular Biology 2020Quote: ... total DNA was purified from supernatant using commercially available kit (DNeasy 96 Blood & Tissue kit, Qiagen). The HBV DNA was amplified in a real-time PCR assay using LightCycler 480 (Roche ...