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Citations for Qiagen :
2201 - 2250 of 10000+ citations for C Reactive Protein CRP ELISA Kit 5 Plate since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2024Quote: ... the supernatant was loaded onto a 5-mL column of Ni-NTA agarose (Qiagen, Inc.) pre-equilibrated with buffer A ...
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bioRxiv - Microbiology 2019Quote: ... DNA was extracted from each section individually using the protein precipitation method (Puregene, Qiagen, Netherlands).
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bioRxiv - Biophysics 2019Quote: ... proteins were purified by batch Ni-NTA bead purification (1 mL slurry/1L culture; Qiagen) and further purified by a Superdex 200 16/60 column (GE Healthcare ...
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bioRxiv - Pharmacology and Toxicology 2019Quote: ... Uncleaved proteins and TEV were removed by a second pass with Ni-NTA resin (Qiagen). Proteins were concentrated and further purified using gel filtration (S75 16/60 ...
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bioRxiv - Cell Biology 2021Quote: ... CRY2 protein was isolated by Ni2+-nitrilotriacetic acid (Ni-NTA) affinity chromatography (Qiagen, Hilden, Germany) following elution with 50 mM Tris buffer pH 7.5 ...
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bioRxiv - Biochemistry 2020Quote: ... The His-tag protein was purified by using a column of Ni-Sepharose 4B (Qiagen), followed by gel filtration using a Superdex 75 column (G.E ...
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bioRxiv - Systems Biology 2020Quote: ... Upstream regulation and protein networks were evaluated via Ingenuity Pathway Analysis (IPA, QIAGEN, Hilden, Germany) based on the DEPs in the plasma experiment ...
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bioRxiv - Microbiology 2021Quote: ... The OrhR-His6 fusion protein was purified to homogeneity using Ni-NTA Spin Columns (Qiagen) and dialyzed against binding buffer ...
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bioRxiv - Cell Biology 2022Quote: ... The proteins were purified under native conditions by affinity chromatography using Ni–NTA agarose (Qiagen) following the manufacturer’s instructions.
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bioRxiv - Plant Biology 2022Quote: ... and the recombinant protein was purified on Ni-NTA agarose following the manfacturer’s instructions (Qiagen). The eluate (3x 500 μl ...
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bioRxiv - Microbiology 2020Quote: ... and proteins were purified from clarified lysate by affinity chromatography on Ni2+-nitrilotriaceta-agarose (Qiagen) in 20 mM Tris-HCl 500 mM NaCl pH 8.0 buffer and by size exclusion chromatography on Superdex 200 HL 16/600 column (GE Healthcare ...
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bioRxiv - Microbiology 2019Quote: ... The recombinant Lsr2 proteins were purified using immobilized-metal affinity chromatography (Ni-NTA agarose, Qiagen) and size exclusion chromatography ...
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bioRxiv - Biophysics 2019Quote: ... All the proteins were obtained in cell lysates and subsequently purified using Ni-NTA (Qiagen) affinity-columns and superdex 200 increase 10/300 GL (GE Lifesciences ...
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bioRxiv - Molecular Biology 2019Quote: ... His6- and GST-tagged proteins were affinity-purified in batch on Ni-NTA agarose (Qiagen) and glutathione Sepharose 4 Fast Flow (GE Healthcare) ...
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bioRxiv - Microbiology 2020Quote: ... The histidine-tagged proteins MprA and MprA* were purified using Ni-NTA Agarose beads (Qiagen) using standardized protocol(Chadha et al. ...
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bioRxiv - Microbiology 2020Quote: ... 6x Histagged proteins were purified from the crude extract using Ni-NTA agarose columns (Qiagen) according manufacturer’s instructions ...
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bioRxiv - Microbiology 2021Quote: ... NspS-6xHis protein was purified from the clarified supernatant using Ni-NTA Superflow resin (Qiagen) equilibrated in lysis buffer ...
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bioRxiv - Cancer Biology 2022Quote: Breast tumor tissues (PTs and IBTRs) were processed using the AllPrep DNA/RNA/Protein (Qiagen) protocol ...
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bioRxiv - Biochemistry 2022Quote: ... His-tagged CSF3R protein was enriched by nickel-nitrilotriacetic acid (Ni-NTA) agarose (Qiagen 30210) and further purified by size-exclusion chromatography on a Superdex 75 10/300 GL column (Cytiva 17517401 ...
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Structure and Neutralization Mechanism of a Human Antibody Targeting a Complex Epitope on Zika VirusbioRxiv - Microbiology 2022Quote: ... Recombinant Fab proteins were purified from the culture supernatant by nickel-nitrilotriacetic acid agarose (Qiagen). Recombinant mAbs were affinity purified by MabSelect resin (Cytiva ...
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bioRxiv - Neuroscience 2023Quote: ... The list of quantified proteins was uploaded into IPA software (Qiagen, Redwood City, CA, USA) for further analyses ...
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bioRxiv - Synthetic Biology 2023Quote: ... All proteins were purified by gravity flow Ni2+ -NTA affinity chromatography (Qiagen, Cat. -No. 30230), except TEV-construct which was purified by gravity flow Strep-Tactin®XT Superflow® high-capacity resin (IBA Cat ...
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Phospho-KNL-1 recognition by a TPR domain targets the BUB-1–BUB-3 complex to C. elegans kinetochoresbioRxiv - Cell Biology 2024Quote: ... 6xHis tagged proteins were purified from the clarified lysates by using Ni-NTA beads (Qiagen), and incubated with end-over-end rotation overnight at 4°C ...
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bioRxiv - Cell Biology 2023Quote: ... The supernatant lysates were purified on Ni-NTA agarose beads (for His6-tagged proteins, Qiagen) or Glutathione High Capacity Magnetic Agarode Beads (for GST-tagged proteins ...
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bioRxiv - Cell Biology 2023Quote: All constructs for bacterial protein expression were cloned in the pQE plasmid (Qiagen, Valencia, CA). WT and mutant TTC1 were cloned downstream of a His14-bdSUMO-GFP tag ...
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bioRxiv - Immunology 2023Quote: ... the proteins were purified by Ni-NTA affinity chromatography using Ni-NTA agarose beads (Qiagen) and gel filtration with a HiLoad 16/600 Superdex 75 pg column in buffers containing 20 mM HEPES pH 7.4 ...
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bioRxiv - Biophysics 2024Quote: DR2539 truncated protein was initially screened by the following commercial crystallization screens: Classics Suite (Qiagen), Salt grid (Hampton Research) ...
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bioRxiv - Genetics 2024Quote: ... Proteins were harvested 60 h after infection and purified with Ni-NTA Superflow resin (Qiagen), anion-exchange chromatography (Source 15Q ...
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bioRxiv - Biophysics 2024Quote: 6xHis-SUMO-Cys-GAF-DBD protein was first purified on Ni-NTA agarose (Qiagen 30210). Briefly ...
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bioRxiv - Biophysics 2024Quote: ... The recombinant proteins were purified from the supernatants using Ni-NTA agarose affinity columns (Qiagen). The protein concentration was measured using the Bradford method (protein assay kit ...
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bioRxiv - Biochemistry 2024Quote: ... The protein was purified by incubation with nickel-nitriloacetic acid (Ni-NTA) agarose resin (Qiagen) for 3h at 4 °C ...
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bioRxiv - Biophysics 2019Quote: ... Lysates were spun for a further 20 min at 40,000 g at 4 °C and resulting supernatants were incubated 1 hour with Ni2+-nitrilotriacetate (NTA) agarose beads (QIAGEN) at 4 °C on rotating wheel ...
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bioRxiv - Microbiology 2019Quote: ... Cells were washed with PBS and incubated at 37°C with a 1:100 dilution of RNase A (QIAGEN) in PBS for 3 hours ...
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bioRxiv - Genomics 2022Quote: ... supplemented with 100 μg/mL of carbenicillin for 16 hr at 30°C followed by plasmid purification (Qiagen, 12991).
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bioRxiv - Biochemistry 2020Quote: ... Lysates were centrifuged at 30,597xg and 4°C for 20min and the supernatant was bound to Ni-NTA Agarose resin (Qiagen), washed with 10 CV of wash buffer (50 mM HEPES-KOH pH=7.5 ...
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bioRxiv - Genetics 2019Quote: Single cells meant for RNA processing were sorted into 96-well full-skirted Eppendorf plates that were pre-chilled at 4°C and were prefilled with 10μL of lysis buffer consisting of TCL buffer (Qiagen) supplemented with 1% beta-mercaptoethanol ...
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bioRxiv - Microbiology 2019Quote: ... Cell lysates were then incubated at 4 °C for 1-1.5 hours on one ml of Ni-NTA resin (Qiagen). The resin was then loaded into columns (Biorad ...
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bioRxiv - Biochemistry 2020Quote: ... The lysate was cleared using a JA-20 rotor (18,000 rpm for 30 min at 4°C) and incubated with Ni-NTA superflow resin (Qiagen) for 30 min ...
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bioRxiv - Cell Biology 2021Quote: Tissue lysates were further homogenized and centrifuged at 10,000 g for 10 min at 4°C in microcentrifuge spin columns (QIAshredder, Qiagen) to obtain clear protein lysate ...
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bioRxiv - Plant Biology 2021Quote: ... the protein was removed by 1.1 μL of proteinase K (20mg/ml) at 45° C for 1h and RNA by 2μL of RNaseA (1mg/ml) (Qiagen) digestion at 37°C for 1h ...
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bioRxiv - Plant Biology 2020Quote: ... 10mM imidazole was added and incubated on rotator for overnight at 4°C in the presence of 2.5ml of Ni-NTA beads (Qiagen). The beads were washed with EB containing 6M guanidine-HCl and 0.25% Triton X-100 ...
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bioRxiv - Microbiology 2020Quote: ... Crystals of Brd4(BD1) with YFV-C (1-10) K4ac peptide were grown from a solution containing 0.2 M sodium chloride (QIAGEN), 0.1 M Tris (pH 7.0 ...
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bioRxiv - Biochemistry 2022Quote: ... Lysate was cleared by centrifugation (20 min, 20,000 × g, 4°C) and clarified lysate was purified on Ni-NTA resin (QIAGEN) eluting in buffer A supplemented with 200 mM imidazole in gravity flow ...
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bioRxiv - Molecular Biology 2022Quote: ... counted and PBS washed pellets were stored at -80 °C for subsequent gDNA extraction (QIAamp DNA Mini, QIAGEN 51304). DNA content was determined with Qubit dsDNA HS Kit (Thermo Fisher Q32854 ...
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bioRxiv - Biochemistry 2022Quote: ... Lysate was cleared by centrifugation (20 min, 20,000 × g, 4°C) and clarified lysate was purified on Ni-NTA resin (QIAGEN) eluting with buffer A supplemented with 250 mM imidazole in gravity flow ...
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bioRxiv - Biochemistry 2022Quote: ... 0.5 mM GDPNP-Mg2+) were obtained at 4°C using 0.2 M Lithium nitrate and 20% PEG3350 (PEG Suite I, Qiagen) as precipitating agent ...
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bioRxiv - Biochemistry 2022Quote: ... Lysate was cleared by centrifugation (20 min, 20,000 × g, 4°C) and clarified lysate was subjected to Ni-NTA resin (QIAGEN), washed and protein eluted with buffer B supplemented with 250 mM imidazole ...
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bioRxiv - Biochemistry 2022Quote: ... cleared by centrifugation (20 min, 20,000 × g, 4°C) and the clarified lysate was purified on Ni-NTA resin (QIAGEN) eluting in buffer C supplemented with 200 mM imidazole ...
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bioRxiv - Biochemistry 2022Quote: ... Lysate was cleared by centrifugation (20 min, 20,000 × g, 4°C) and clarified lysate was purified on Ni-NTA agarose resin (QIAGEN) eluted with buffer A supplemented with 0.5 mM TCEP and 200 mM imidazole ...
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bioRxiv - Biochemistry 2022Quote: ... Lysate was cleared by centrifugation (20 min, 20,000 × g, 4°C) and cleared lysate was subjected to Ni-NTA resin (QIAGEN), washed and protein eluted with buffer B (20 mM Tris-HCl pH 8.0 ...