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Citations for Qiagen :
2251 - 2300 of 2514 citations for 7 Bromo 2 Methyl 1 Indanone since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2023Quote: ... TbFUT1 cKO and TbFUT1 cKO F1-γL262P/WT cells grown under permissive and non-permissive conditions and RNA was extracted from 1 ×107 cells using RNeasy RNA extraction kit (Qiagen). RT-qPCRs were performed using Luna® Universal qPCR Master Mix (NEB ...
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bioRxiv - Microbiology 2023Quote: DNA was extracted from enrichment material in technical duplicates (1 ml digestate or 0.25 g soil) using the PowerLyzer™ Soil DNA extraction kit (QIAGEN) with modified bead beating using a MP Biomedicals™ FastPrep®-24 (Thermo Fischer Scientific Inc ...
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bioRxiv - Microbiology 2023Quote: ... viral nucleic acids were extracted using the QIAamp Viral RNA Mini Kit (QIAGEN GmbH, QIAGEN Strasse 1, 40724 Hilden, Germany), which purifies RNA and DNA ...
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bioRxiv - Microbiology 2023Quote: ... a single GFP+ or GFP-J-Lat 11.1 cell was sorted directly into each well of a 96-well PCR plate containing the pre-amplification mastermix consisting of 1 μL One-step RT-PCR enzyme (QIAGEN), 5 μL 5× One-step RT-PCR buffer (QIAGEN) ...
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bioRxiv - Genomics 2023Quote: ... The samples were rinsed with 4× SSC and hybridized with RNA seqFISH primary probe pools (1-10 nM per probe) and 10 nM polyT LNA oligo (Qiagen) in a 50% hybridization buffer consisting of 50% formamide (Invitrogen AM9342) ...
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bioRxiv - Cancer Biology 2023Quote: ... a ∼20 mg chunk of frozen lung tissue was collected using a scalpel and placed in 1 mL RLT buffer (Qiagen) with 1:100 β-mercaptoethanol in a Precellys silicone bead dissociation tube (Bertin-Corp) ...
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bioRxiv - Microbiology 2023Quote: ... The protein concentrations of all the collected samples were adjusted to 400 μg mL−1 using the Qubit protein broad range assay kits (Qiagen). The Cyclic AMP XP® kit ELISA plates were prepared according to the manufacturer’s protocols ...
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bioRxiv - Microbiology 2023Quote: ... and the purity of the purified proteins was checked by SDS-PAGE and western blot using anti-His antibody (1:1000, Qiagen) as primary antibody ...
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bioRxiv - Genomics 2023Quote: ... The bead-bound gDNA was isothermally amplified for 3 hours at 30 °C then 10 minutes at 65 °C using a miniaturised (1/5 vols) Repli-g Single-Cell assay (Qiagen). The amplified gDNA was cleaned up with 0.8 × vols Ampure XP and 80 % ethanol ...
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bioRxiv - Biochemistry 2023Quote: Total RNA was isolated and purified from cultured cells using a standard commercial RNA prep kit (#48500, Norgen) and 1 μg of RNA was reverse transcribed to cDNA using a cDNA synthesis kit (#205314, Qiagen). 10 ng of cDNA was used for quantitative PCR by SYBR Green ...
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bioRxiv - Plant Biology 2023Quote: Frozen tissue was ground into a fine powder using a ⅛” steel ball bearing with 1 min shaking at 25 Hz in a TissueLyser II (Qiagen). Total RNA was extracted from finely ground tissue using TRI reagent (Sigma-Aldrich ...
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bioRxiv - Cancer Biology 2022Quote: ... reverse transcription of RNA extracted from OECM-1 cells transfected with miRNA mimics and SC control was carried out using the Quantitect Reverse Transcription Kit (QIAGEN) according to the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2022Quote: Genomic DNA was isolated from 1 million cells of each cell line using the QIAamp®DNA Micro Kit (QIAGEN) following the manufacturer’s guidelines ...
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bioRxiv - Cancer Biology 2023Quote: Total RNA was isolated from DLD-1 cells ectopically expressing EPHB1 mutants using the RNeasy Plus Mini Kit (Qiagen, Germany). First strand cDNA synthesis was conducted with the RevertAid H-minus First Strand cDNA synthesis kit (Thermo Fisher Scientific ...
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bioRxiv - Evolutionary Biology 2023Quote: ... Prior to RNA extraction individuals were homogenized in 200 μl homogenization buffer including 4 μl 1-Thioglycerol using a TissueLyser II (Qiagen) with a mixture of five 1 mm zirconia (BioSpec Products ...
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Human CCR6+ Th cells show both an extended stable gradient of Th17 activity and imprinted plasticitybioRxiv - Immunology 2023Quote: ... CCR6int and CCR6high subsets of CD4+ T cells were used for isolating RNA without and with prior activation with 20 ng/ml PMA and 1 mM ionomycin for 3 h at 37°C under 5% CO2 using RNeasy Mini Kit (Qiagen) and gene expression levels were determined using Illumina Human WG6 Expression BeadChip Version 3 arrays (Illumina Inc. ...
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bioRxiv - Immunology 2023Quote: Total RNA was extracted from frozen tissue samples using ceramic beads (Precellys homogenizer, 1× 20 s) in RLT buffer (RNeasy Mini kit, Qiagen) supplemented with 1%ß-mercaptoethanol ...
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bioRxiv - Immunology 2023Quote: ... Cells were stimulated with LPS (1 μg/ml) for 6 hours at 37°C followed by RNA isolation using a RNeasy Kit (QIAGEN). Extracted RNA was converted to cDNA with an iScript cDNA Synthesis Kit (Bio-Rad) ...
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bioRxiv - Neuroscience 2023Quote: ... brain hemispheres were homogenized in freshly prepared lysis buffer (0.6 ml per 30 mg of brain tissue) containing 1% β-mercaptoethanol using a TissueRuptor homogenizer (Qiagen).
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bioRxiv - Synthetic Biology 2023Quote: ... The organs were weighed and lysed in 1 mL Triton X-100 solution (0.1% in PBS) using a TissueLyser II (QIAGEN, Germany) according to the manufacturer protocol ...
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Analysis of spike protein variants evolved in a novel mouse model of persistent SARS-CoV-2 infectionbioRxiv - Microbiology 2023Quote: ... 45 cycles at 95° for 15 sec and 60° for 1 min were performed in the Rotor-Gene Q real-time PCR cycler (Qiagen). The numbers of copies of the RdRP gene in the samples were calculated using an RdRP cDNA standard curve.
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bioRxiv - Cell Biology 2023Quote: ... and purified His-Cdk9 (1 μM) in total volume of 300 μl were added to 50 μl of Ni-NTA agarose (Qiagen) equilibrated with binding buffer 1 (25 mM Tris-HCl pH 7.5 ...
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bioRxiv - Genetics 2023Quote: ... Amplicons spanning the gRNA target sites were amplified from 1 μL of purified gDNA using HotStar PCR Master Mix (Qiagen). Half of each reaction was run on a 1.5% agarose gel ...
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bioRxiv - Synthetic Biology 2024Quote: ... Illumina sequencing-compatible Unique Dual Index adapters were ligated onto the pools using the QIAseq 1-step amplicon library kit (Qiagen). Library quality control was performed using Qubit and TapeStation and sequenced on Illumina MiSeq platform to generate 2 × 250 bp reads.
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bioRxiv - Systems Biology 2024Quote: ... and left brain hemisphere using the phenol-chloroform extraction method with 1 mL of Qiazol Lysis Reagent (Qiagen, Hilden, Germany), as previously described in Ederer et al ...
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bioRxiv - Molecular Biology 2023Quote: ... Worms were snap frozen in liquid nitrogen before being ground into a fine powder for 1 min by a stainless-steel bead in a TissueLyser LT (Qiagen) operating at 50 oscillations per second ...
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bioRxiv - Biochemistry 2024Quote: ... The remaining supernatant was centrifuged again at 5,000 x g for 20 minutes at 4°C and equilibrated for 1 hour in 3 mL of Ni-NTA resin (Qiagen) that was pre-equilibrated in the extraction buffer ...
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bioRxiv - Microbiology 2023Quote: ... Metagenomic sequencing libraries were constructed from extracted DNA samples with 10 μL (1/5 volume) reactions using the QIAseq FX DNA Library Kit (QIAGEN). Each metagenomic sequencing library was sequenced using the Illumina NextSeq 2000 System 2 x 150 bp configuration.
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bioRxiv - Microbiology 2023Quote: ... DNA extraction aimed at producing fragments adequate for long-read sequencing and followed either of two methods: 1) DNeasy PowerSoil Kit (Qiagen) without bead-beating and vortexing steps ...
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bioRxiv - Microbiology 2023Quote: ... round 1 and 3 virus infections of GeCKO-A549 cells using the midi gDNA extraction kit (Qiagen, Germantown, MD, USA). The sgRNA’s DNA copies were PCR amplified from the extracted gDNAs for next generation sequencing (Fig 1) ...
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bioRxiv - Microbiology 2023Quote: ... Cells were incubated at 37 °C with agitation for six hours after which time 1 mL cell pellets were treated with RNAprotect (Qiagen). Cell pellets were washed in 0.5 mL PBS pH 7.4 ...
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bioRxiv - Microbiology 2023Quote: ... pHIVec2.luc reporter plasmid and psPAX2 packaging plasmid (catalog number 11348, NIH AIDS Reagent Program) in a ratio of 1:6:3 using either Effectene (Qiagen) or calcium phosphate ...
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bioRxiv - Biochemistry 2023Quote: ... about 100 mg of tissue samples were homogenised in 1 mL of TRI reagent at room temperature using a tissue lyser (Qiagen). The homogenised samples were transferred to fresh microcentrifuge tubes for RNA isolation ...
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bioRxiv - Biochemistry 2023Quote: ... HBEC5i cells with treated with either non-specific siRNA or Flvcr2 siRNA were harvested and kept in 1 mL TRI reagent (Qiagen).
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bioRxiv - Bioengineering 2023Quote: ... Lysate supplemented with 20 mM imidazole was cleared by centrifugation at 15000 g at 4 °C for 30 minutes and the supernatant was then loaded onto 1 mL bed volume of Ni-NTA agarose beads (Qiagen) pre-washed with 5 column volumes of 1x TBS (pH 7.3) ...
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bioRxiv - Bioengineering 2023Quote: ... the cells were pelleted at 15000 g at 4 °C for 30 minutes and the supernatant was supplemented with 20 mM of imidazole before loading onto 1 mL bed volume of Ni-NTA agarose beads (Qiagen) pre-washed with 5 column volumes of 1x TBS (pH 7.3) ...
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bioRxiv - Microbiology 2023Quote: ... Illumina sequencing-compatible Combinatorial Dual Index (CDI) adapters were ligated onto pooled amplicons using the QIAseq 1-step amplicon library kit (Qiagen). Library QC was performed using Qubit and Tapestation and sequenced on Illumina MiSeq platform to generate 2×250bp reads ...
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bioRxiv - Genetics 2023Quote: ... Cells were washed three times with ice-cold PBS and lysed at room temperature with 1 ml/well QIAzol lysis reagent (QIAGEN). Lysates were incubated at room temperature for 5 min and homogenised by pipetting up and down ...
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bioRxiv - Bioengineering 2023Quote: ... The supernatants were then supplemented with 20 mM imidazole and then loaded onto 1 mL bed volume of Ni-NTA agarose beads (Qiagen) that were pre-washed with 5 column volumes of 1x PBS ...
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bioRxiv - Plant Biology 2023Quote: ... Genomic DNA libraries were created using genomic DNA extracted from 2-week old Col-0 seedlings grown on ½ Murashige and Skoog media supplemented with 1% sucrose with DNeasy Plant Mini kit (Qiagen). Plasmids containing the proteins of interest in the pIX-HALO backbone were ordered from ABRC ...
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bioRxiv - Microbiology 2023Quote: Total genomic DNA was extracted from HSV-1 ANG and ANG path virions using the QiaAmp Mini DNA Kit (Qiagen). Full-length nucleotide sequences of UL44 ...
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bioRxiv - Microbiology 2023Quote: ... Illumina sequencing-compatible Unique Dual Index (UDI) adapters were ligated onto the pools using the QIAseq 1-step amplicon library kit (Qiagen). Library QC was performed using Qubit and Tapestation and sequenced on the Illumina MiSeq platform to generate 2×250bp reads.
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bioRxiv - Molecular Biology 2023Quote: cDNA was produced from 1 μg of total RNA (final cDNA concentration 5 ng/ml) using QuantiTech Reverse Transcription kit (Qiagen) according to the manufacturer’s instructions.
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bioRxiv - Genomics 2024Quote: Genomic DNA was extracted from 30-70 mg of tissue or 5.0 x 10^6 cells for Source 1 cell lines using commercially available QIAGEN QIAamp Mini Kit (QIAGEN, 51304), following the manufacturer’s protocol ...
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bioRxiv - Neuroscience 2024Quote: ... we added 1 µl of the spike-in mix provided in the QIAGEN RNA Spike-In Kit (Cat. No.: 339390, QIAGEN) to the tube ...
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bioRxiv - Pathology 2024Quote: ... liver was homogenized in 500uL of 3:1:6 isopropanol:water:ethyl acetate containing internal standard in ceramic bead tubes (Qiagen #13113-50) using the TissueLyzer II (Qiagen #9244420) ...
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bioRxiv - Microbiology 2024Quote: ... The DNA of the 20 Jan and 3 Mar 2021 samples (1/3 pellet) was extracted with the DNeasy UltraClean Microbial Kit (Qiagen) following the manufacturer’s instructions ...
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bioRxiv - Immunology 2024Quote: ... and homogenized via sonication at 20 kHz for 1 min before RNA isolation was performed using a RNeasy Mini Kit (Qiagen). For qPCR ...
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bioRxiv - Biochemistry 2024Quote: ... and the supernatant was subjected to immobilized metal affinity chromatography using a 1 mL Ni-NTA agarose column (Qiagen, 30210) equilibrated with binding buffer ...
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bioRxiv - Molecular Biology 2024Quote: ... The reactions were incubated for 15 min at 60°C and then terminated by adding 1 μL 5 M NaOH to degrade RNA and heating at 95 °C for 5 min followed by neutralization with 1 μL 5 M HCl and one round of MinElute column clean-up (Qiagen). The R1R DNA adapter was adenylated by using a 5’ DNA Adenylation kit (New England Biolabs ...