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Citations for Qiagen :
2151 - 2200 of 2514 citations for 7 Bromo 2 Methyl 1 Indanone since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2020Quote: DNA extraction was done from 1 ml of the different sample types using QIAamp DNA mini kit (Qiagen, Hilden, Germany), as per the vendor’s instructions with slight modifications ...
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bioRxiv - Molecular Biology 2020Quote: ... catalysis was measured using a previously described assay (22) in which hexahistidine-tagged PARP-1 was immobilized on Ni(II)-NTA-coated plates (Qiagen) in the presence of NAD+/biotinylated-NAD+ (Trevigen) ...
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bioRxiv - Microbiology 2020Quote: ... HIV-1 integration was examined in 293T cells and J-Lat cells by isolating genomic DNA from 1 × 106 cells using the DNeasy Tissue Kit (Qiagen). Alu-LTR sequences were amplified during first round PCR from 100 ng of total genomic DNA using the following primers ...
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bioRxiv - Evolutionary Biology 2020Quote: ... several loci were amplified simultaneously from 1 μl of extracted DNA using 5 μl of the Multiplex PCR Master Mix (Qiagen) and varying amounts of the pooled primer mixes (Pool A ...
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bioRxiv - Physiology 2021Quote: ... Shredded bone marrow cells were frozen (−80°C) in RLT buffer (1% beta-mercaptoethanol) until RNA extraction using RNAeasy mini kit (Qiagen). RNA integrity number (RIN ...
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bioRxiv - Immunology 2020Quote: ... mRNAs recovered from microdissected samples or vaginal biopsies (about 1 mm3) were reverse-transcribed with the QuantiTect Rev Transcription Kit (Qiagen), used according to the manufacturer’s recommendations ...
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bioRxiv - Microbiology 2020Quote: ... Glass beads (3mm) and 1 mL of sterile water were added for homogenization at 30Hz for 10 minutes (TissueLyser II, Qiagen). Homogenates were diluted with PBS and inoculated onto SAB plates and incubated for 3 days at 30°C ...
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bioRxiv - Microbiology 2020Quote: ... Preparation of total RNA and Northern blotting including the determination of RNA half-lives were carried out as described previously [29] except that 1 ml time samples were taken and directly added to 250 μl RNAprotect Bacteria Reagent (Qiagen) or to 250 μl containing 5 % phenol and 95 % ethanol ...
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bioRxiv - Microbiology 2020Quote: ... The PCR fragment was then digested with Dpn I at 37 °C for 1 h and purified using the QIAquick PCR purification kit (Qiagen). To prepare the competent SW102 containing the pSMART BAC-T7-scv2-replicon ...
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bioRxiv - Microbiology 2021Quote: ... samples were weighed and homogenized in DMEM containing 10% FBS and 1% antibiotics using 5 mm stainless steel beads (Qiagen) and the TissueLyser II (Qiagen) ...
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bioRxiv - Bioengineering 2021Quote: ... The plasmid coding for FLP recombinase and the mCherry reporter or 47bp-insertion TLR reporter plasmid were transfected into the cells at a 9:1 ratio using polyfect (QIAGEN) according to the manufacturer’s protocol (900ng mCherry reporter or 47bp-insertion TLR reporter plasmid and 100ng FLP recombinase plasmid to make 1 μg plasmid in total) ...
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Glutaric aciduria type 3 is a naturally occurring biochemical trait in inbred mice of 129 substrainsbioRxiv - Biochemistry 2020Quote: ... were homogenized in ice-cold RIPA buffer supplemented with protease inhibitor cocktail (∼20 mg tissue per 1 mL lysis buffer) using a TissueLyser II apparatus (Qiagen) and the homogenates were centrifuged at 1,000xg ...
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bioRxiv - Animal Behavior and Cognition 2020Quote: ... we transfected S2 cells in each well of a 6-well plate with 1 µg each of HA/GFP-tagged constructs using Effectene (Qiagen). After incubation for 3 days ...
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bioRxiv - Bioengineering 2021Quote: ... for 1 day prior to extraction of total RNA of the treated leaves with an RNeasy plant mini kit (QIAGEN). Two-step qPCR was performed to quantify GFP gene silencing using the total RNA extracted from leaves ...
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bioRxiv - Cell Biology 2021Quote: ... The remaining lysate was transferred to a 15 ml falcon tube and 1 ml of a 50% slurry consisting of NiNTA agarose beads (Qiagen) in resuspension buffer was added per 10 ml of lysate ...
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bioRxiv - Genomics 2021Quote: ... The PCR products were size-selected on a 1% agarose gel and purified using the MinElute PCR Purification Kit (Qiagen). The pSTARR-seq_human vector (kindly provided by Alexander Stark ...
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bioRxiv - Immunology 2020Quote: RNA was extracted from isolated neutrophils after the 1 and 4 hour EC challenges as well untreated controls using the miRNeasy Micro kit from Qiagen and libraries were generated using KAPA PolyA enrichment mRNA library prep ...
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bioRxiv - Biophysics 2019Quote: ... Insoluble material was pelleted for 1 hr at 35,000 rpm and supernatant was added to Ni-NTA resin (QIAGEN, 30410) in batch and rotated at 4°C for 1 hr ...
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bioRxiv - Molecular Biology 2019Quote: ... PCR products were electrophoresed in a 1% agarose gel and the gel bands were isolated using QIA Quick PCR Purification Kit (Qiagen). Isolated DNA was cloned into pCR-TOPO plasmids using the TOPO TA cloning kit (ThermoFisher Scientific) ...
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bioRxiv - Synthetic Biology 2019Quote: ... grown over night in 5 mL LB and 100 μg mL-1 ampicillin and DNA were purified using a QIAprep Spin Miniprep Kit (QIAGEN). Sequencing was performed by GATC using the primers in SI Appendix Table S4 ...
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bioRxiv - Cancer Biology 2019Quote: Genomic DNA was extracted from up to 1 mL whole blood or buffy coat using the Gentra Puregene Blood Kit (Qiagen) as per the manufacturer’s protocol ...
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bioRxiv - Microbiology 2019Quote: ... an 8 μg of the isolated RNA was further treated with 2U of TURBO DNase (Invitorgen) at 37°C for 1 h and purified with RNeasy spin columns (Qiagen). The amount of RNA was quantified with Qubit RNA BR assay kit (Invitrogen) ...
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bioRxiv - Biochemistry 2019Quote: ... The template was run on a 1% agarose gel and extracted using a QIAquick Gel Extraction kit (Qiagen, Hilden, Germany). DNA template concentration was determined by a Qubit 3.0 Fluorometer (Life Technologies ...
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bioRxiv - Genomics 2021Quote: ... This was followed by RNase A treatment for 1 h at 37°C and purification using MinElute PCR purification columns (Qiagen). cDNA amplification was performed with reporter-specific nested cDNA primers (Oligos 16 and 17 for libraries in vectors d and e ...
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bioRxiv - Genomics 2021Quote: ... To eliminate lipids supernatants were applied to a RNeasy column and centrifuged at 10,000 rpm and 4 °C for 1 min (Qiagen, 74104). The flow through was collected and protein concentrations were assessed using the Bradford assay ...
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bioRxiv - Immunology 2021Quote: ... Complementary deoxyribonucleic acid (cDNA) was synthesised from every 1 μg of total mRNA in 20 μL volume with QuantiTect Reverse Transcription Kit (Qiagen) according to the manufacture protocol ...
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bioRxiv - Biochemistry 2021Quote: pAC5.1 vectors with CLIP-CRY and TIM-SNAP-HA were delivered into s2 cells using Effectene reagent (Cat# 301425, Qiagen) or TransIT-Insect Transfection Reagent (Cat# MIR6100 ...
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bioRxiv - Molecular Biology 2021Quote: Total RNA was purified from Achilles enthesis of 1-dpi pups or from tissue culture cells using the RNeasy Kit (Qiagen). Reverse transcription was performed with High-Capacity cDNA Reverse Transcription Kit (Applied Biosystems ...
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bioRxiv - Cancer Biology 2021Quote: ... The LNA gapmers for lnc-FLii-1 and lnc-LSAMP-3 were designed and purchased along with negative control LNA from Qiagen. For transfection ...
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bioRxiv - Cancer Biology 2021Quote: The frozen tumors were minced to small pieces and suspended in buffer P1 (QIAGEN, 1 mL Buffer/100 mg tumor) supplemented with 100 μg/mL RNase A (Promega) ...
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bioRxiv - Genomics 2022Quote: ... The indexed PCR products were cleaned using 1 Volume AMPure XP beads on magnetic stand and eluted with EB buffer (QIAGEN) followed by quantification using a Qubit dsDNA HS Assay Kit ...
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bioRxiv - Developmental Biology 2021Quote: ... Skin samples were taken by remote biopsy dart (1 mm wide by 20-25 mm deep plug) and preserved in RNAlater (Qiagen). Blood samples were taken opportunistically during veterinarian visits and preserved in EDTA tubes ...
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bioRxiv - Cell Biology 2021Quote: ... and were then lysed in the plate using 1 mL of RTL buffer from an AllPrep DNA/RNA/Protein isolation kit (Qiagen). Experiments were performed in triplicate.
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bioRxiv - Genetics 2021Quote: ... In vitro transcribed mRNAs were DNAse treated using 1 μl TURBO-DNAse for 20 minutes at 37 °C and purified using RNeasy Mini Kit (Qiagen). mRNA product was quantified using NanoDrop (Thermo Fisher ...
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bioRxiv - Microbiology 2021Quote: ... protocol 1 in the RNAprotect Bacteria Reagent Handbook (Enzymatic lysis of bacteria) was followed using the RNeasy Plus Mini Kit (Qiagen) using TE buffer (10 mM Tris-HCl ...
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bioRxiv - Microbiology 2021Quote: ... 200 mg of thawed and mixed faecal samples were added to 1 ml of InhibitEX Buffer (QIAGEN, GmbH, Hilden, Germany) followed by the beat-beating step in TissueLyser II (Qiagen ...
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bioRxiv - Microbiology 2020Quote: ... and 18 centimeters below seafloor (cmbsf) from station AC of Van Keulenfjorden (Supp. Table 1) using the DNeasy PowerSoil Kit (Qiagen) according to the manufacturer’s protocol ...
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bioRxiv - Immunology 2021Quote: ... ~100 mg tissue was homogenized in 1 mL Qiazol and RNA was extracted using RNeasy Lipid Tissue Mini Kit (Qiagen) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2021Quote: ... PCR reactions were run on 1 μg of RNA per sample to afford cDNA using the QuantiTect Reverse Transcription kit (Qiagen). RT-qPCR was performed on an Applied Biosciences 7500 Real-Time PCR system following the QuantiTect SYBR Green PCR kit protocols (Qiagen ...
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bioRxiv - Immunology 2021Quote: Heat-inactivated human serum was diluted 1:10 in phosphate buffered saline (PBS) and applied to a gravity polypropylene flow column (Qiagen) containing 1 mL of Protein G-sepharose resin (Invitrogen ...
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bioRxiv - Microbiology 2021Quote: DNA extraction from 1 mL anaerobic digester sludge was performed using the DNeasy PowerSoil DNA extraction kit (Qiagen, Valencia, CA). DNA from Defo and Diel cultures was extracted from 5 mL culture suspensions collected onto 0.22 µm Durapore membrane filters (Millipore ...
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bioRxiv - Genomics 2021Quote: Genomic DNA was isolated from a suspension of 1 x 106 PBMCs from each individual in the study using a DNA isolation kit (Qiagen). Genotyping was then performed using the Infinium CoreExome-24 (v1.3 ...
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bioRxiv - Genetics 2019Quote: ... DNA was size fractionated on 1% agarose gels and 500-750 bp DNA fragments were gel extracted with the Qiaquick gel extraction kit (Qiagen). A first round of PCR was performed using primers GSP1 and P1 (First denaturation step ...
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bioRxiv - Genetics 2019Quote: ... for the PCR analysis of potential recombination events genomic DNA from 1×10^6 cells was extracted using DNeasy Blood & Tissue Kits (Qiagen) following the manufacturer’s instructions ...
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bioRxiv - Genomics 2020Quote: ... Amplified DNA (2ul from a 1:20 dilution of a 16 h REPLI-g Mini Kit whole-genome amplification, Qiagen) from each sorted population was tested in a 96-well plate in duplicate according to the manufacturer’s protocol ...
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bioRxiv - Neuroscience 2021Quote: ... and 0.4 mg/mL Proteinase K (55°C for 1 hour) before being purified using the QIAquick PCR purification kit (QIAGEN). qPCR was performed on the Rotor-Gene 3000 (Corbett Life Science ...
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bioRxiv - Cancer Biology 2020Quote: ... or 50 um sections (liver metastases) were cut and homogenized in 1 ml of AllPrep DNA/RNA Mini Kit (Qiagen) using a plastic pestle (Cole-Palmer #44468-23) ...
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bioRxiv - Bioengineering 2022Quote: ... Illumina sequencing-compatible Unique Dual Index (UDI) adapters were ligated onto pools using the QIAsep 1-step amplicon library kit (Qiagen). Library QC was performed using Qubit and Tapestation before sequencing on an Illumina MiSeq platform at the Duchossois Family Institute Microbiome Metagenomics Facility at the University of Chicago ...
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bioRxiv - Microbiology 2022Quote: ... DNA on the exterior of filtered capsids was digested for 1 hour at 25 °C with 20.3 units DNase (Qiagen: 79254) supplemented with 10 mM MgCl2 ...
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bioRxiv - Molecular Biology 2022Quote: ... The PCR products were purified by ethanol precipitation and 1% agarose gel purified using the QIAquick Gel Extraction Kit (Qiagen). Single-round E ...