Labshake search
Citations for Qiagen :
2151 - 2200 of 10000+ citations for C Reactive Protein ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2020Quote: ... Pellets were lysed by vortexing for 1 minute in 350uL cold supplemented RLT buffer (RLT + β-MeOH) at 4°C and lysates homogenized using QIAshredder columns (Qiagen). RNA was then extracted from these samples using the RNeasy Plus Micro kit (Qiagen ...
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bioRxiv - Physiology 2019Quote: ... Methylation status of the individual CpG sites was identified as an artificial C/T SNP using QCpG software (Pyrosequencing, Qiagen, UK). Then % methylation of each CpG site was then calculated as a proportion of the methylated alleles divided by the sum of all methylated and unmethylated alleles ...
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bioRxiv - Bioengineering 2022Quote: ... after which the cells were FACS sorted for high and low reporter signal.The genomic DNA was extracted by lysing the cell pellets for 10 minutes at 56°C in AL buffer (Qiagen, 19075), supplemented with Proteinase K (Invitrogen ...
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bioRxiv - Plant Biology 2022Quote: ... Samples were centrifuged at 14,000 x g for 20 min (4°C) to pellet insoluble cell debris and supernatants were transferred to violet QIAshredder mini spin columns (Qiagen, Australia) and centrifuged again for one minute ...
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bioRxiv - Synthetic Biology 2024Quote: ... of T4 ligation reaction mix was added to NEB Stbl cell (C3040) for transformation and grown at 37°C for the plasmid DNA preparation (Qiagen miniprep). The resulting plasmids were sequence-verified using Sanger sequencing (Genewiz).
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bioRxiv - Microbiology 2024Quote: ... and 10 zirconium beads were added to approximately 5 mg of intestinal tissue stored at -80 °C in RNA later tissue reagent (Qiagen, Germany). A Tissue lyser (Qiagen ...
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bioRxiv - Developmental Biology 2023Quote: Single sorted cells (∼50’000 cells) were lysed in buffer RLT and stored at -80°C until starting the RNA isolation following manufacturer instructions (Qiagen, 74034). cDNA reaction was performed using qScript™ cDNA SuperMix (QuantaBio ...
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bioRxiv - Immunology 2023Quote: For protein production a pT350 plasmid encoding for the construct of interest with a BiP signal sequence in the N-terminal and a double strep-tag in the C-terminal region was co-transfected with a puromycin resistant plasmid (pCoPuro) (61) with effectene reagent (Qiagen, 301425) following manufacturer’s instructions ...
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bioRxiv - Evolutionary Biology 2023Quote: ... following an incubation at 55°C for 1 hr in 450 µl of lysis buffer with proteinase K (QIAGEN, Hilden, Germany). Each colony was genotyped using twelve microsatellite loci69 ...
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bioRxiv - Biochemistry 2023Quote: ... Cell lysates were clarified by additional centrifugation at 14,000 xg for 30 minutes and supernatants were incubated 1 hour at 4 °C with 5 mL of His-Pur™ Ni-NTA resin (Qiagen) previously washed 3 times with 25 mL of Lysis Buffer ...
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bioRxiv - Microbiology 2020Quote: ... to recover parasite material for genomic DNA isolation using a kit (Qiagen DNA Easy Blood and Tissue Kit), according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2021Quote: ... Plasmids and PCR products were purified with QIAprep plasmid Miniprep kit and QiaQuick PCR purification kit respectively (Qiagen). Plasmid Sanger sequencing was performed by Eurofins Genomics ...
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bioRxiv - Evolutionary Biology 2019Quote: ... using a modified Trizol isolation protocol in combination with a column based commercial kit (QIAGEN RNeasy Mini Kit) based on the manufacturer’s protocol ...
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bioRxiv - Physiology 2019Quote: DNA of selected LAB was extracted using DNA extraction kit (QIAamp Blood and Tissue Kit, Qiagen, Hilden, Germany). The amplification of 16SrRNA genes were conducted using 27F 5’-AGAGTTTGATCCTGGCTCAG-3’ and 1492R-5’-GGCTACCTTGTTACGACTT-3’primers ...
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bioRxiv - Microbiology 2021Quote: ... genomic DNA was extracted using the DNAeasy® UltraClean® Microbial kit Isolation Kit (Qiagen Inc., MD, USA) and the near full-length 16S rRNA gene was amplified using primers 8F (5’-AGAGTTTGATCCTGGCTCAG-3’ ...
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Impairment of a distinct cancer-associated fibroblast population limits tumour growth and metastasisbioRxiv - Cancer Biology 2020Quote: RNA was isolated using Qiagen RNeasy kit and cDNA was generated using the QuantiTect reverse transcription kit (Qiagen) according to the manufacturer’s instruction ...
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bioRxiv - Genomics 2020Quote: ... and RNA and DNA were extracted (Qiagen RNEasy Mini Kit #74104/Qiagen DNA Blood and Tissue Kit #69504). Clones were again genotyped to verify absence of the mariner insertion (Fig ...
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bioRxiv - Neuroscience 2021Quote: RNA purification was completed according to the instructions of the manufactured kit (RNeasy® Plus Mini kit; Qiagen). Briefly ...
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bioRxiv - Microbiology 2022Quote: ... against five other extraction kits: the Qiagen MagAttract PowerSoil Pro DNA Isolation Kit (Cat#: 47109; Qiagen, Carlsbad, CA), the Norgen Stool DNA Isolation Kit (Cat# 65600 ...
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bioRxiv - Molecular Biology 2022Quote: ... RT-qPCR reaction was carried out using the QuantiNova Kit Probe RT-PCR Kit (Qiagen, catalog #208354, Germany), using primers and probe described in Naveca et al ...
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bioRxiv - Microbiology 2022Quote: ... The DNA extraction was performed using the PowerSoil DNA extraction kit (DNeasy PowerSoil Pro Kit, QIAGEN, Hilden, Germany), according to the manufacturer’s protocol with an additional 500 μL of 10% (w/v ...
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bioRxiv - Cell Biology 2023Quote: ... Plasmid DNA was extracted using mini-prep plasmid extraction kit and/or Maxi-prep plasmid extraction kit (QIAGEN). The constructs were further confirmed by the Sanger sequencing.
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bioRxiv - Microbiology 2024Quote: ... DNA extraction was performed utilizing commercial kits and following manufacturer’s protocol (Qiagen’s DNeasy 96 Blood & Tissue Kit, Qiagen). The amount and quality of extracted DNA was evaluated using PicoGreen fluorometer and NanoDrop spectrophotometer (ThermoFisher Scientific) ...
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bioRxiv - Microbiology 2024Quote: ... DNA extraction was performed utilizing commercial kits and following manufacturer’s protocol (Qiagen’s DNeasy 96 Blood & Tissue Kit, Qiagen). The amount and quality of extracted DNA was evaluated using PicoGreen fluorometer and NanoDrop spectrophotometer (ThermoFisher Scientific) ...
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bioRxiv - Systems Biology 2023Quote: ... total RNA of the samples was extracted according to manufacturing specifications (RNeasy Protect Bacteria Mini Kit Qiagen kit).
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bioRxiv - Molecular Biology 2023Quote: ... Genomic DNA was extracted using the genomic DNA purification kit (Dneasy Blood and Tissue kit, Cat. 69504, Qiagen), the specific fragment containing rG4-1 sequence was amplified using Phire Green Hot Start II PCR Master Mix (F126S ...
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bioRxiv - Microbiology 2023Quote: Total DNA and mRNA were isolated using QIAamp DNA mini kit and RNeasy mini kit (Qiagen, Germantown, MD), per the manufacturer’s recommendation ...
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bioRxiv - Evolutionary Biology 2023Quote: ... The selected BACs were recovered and sequenced on an Illumina MiSeq using reagent kit Index Nextera XT kit V2-500 cycles and assembled with the CLC Genomics Workbench 20.0 v7.5 (QIAGEN). P ...
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bioRxiv - Molecular Biology 2023Quote: ... All plasmids were purified with a Midiprep kit (ZymoPURE, #D413) or a QIAprep spin miniprep kit (QIAGEN, #27104) and verified by DNA sequencing prior to use ...
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bioRxiv - Genomics 2023Quote: Total RNA from tissues was extracted using a commercial kit (RNeasy Plus Mini Kit; Qiagen, Toronto, ON, Canada). Quality control checks ...
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bioRxiv - Genomics 2023Quote: ... We used the MagAttract® HMW DNA kit and the DNeasy Blood and Tissue kit (Qiagen, Hilden, Germany) for DNA isolation ...
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bioRxiv - Cancer Biology 2024Quote: ... DNA was isolated using either QIAGEN Plasmid Plus Midi Kit or 28 QiaPrep Spin Miniprep Kit (Qiagen, 27104).
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bioRxiv - Genomics 2023Quote: DNA from both types of samples was extracted using a commercially available kit (DNeasy Blood & Tissue Kit, QIAGEN) following the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2024Quote: ... genomic DNA was extracted using the QIAamp DNA Blood Midi Kit or QIAamp DNeasy Blood & Tissue Kit (QIAgen). Genome sequencing libraries were made using Illumina genome DNA prep reagents (Illumina ...
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bioRxiv - Synthetic Biology 2024Quote: ... NEB) and purified using a Qiagen extraction kit as per the manufacturer’s instructions (QIAquick Gel Extraction Kit, Qiagen). A complete list of plasmids included in this study can be found in Related Manuscript File 2 ...
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bioRxiv - Evolutionary Biology 2021Quote: Protein sequence alignments and tree production were made using the CLC main work bench (ver. 5.5.1, Qiagen) software package ...
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bioRxiv - Plant Biology 2019Quote: Sitting-drop vapour-diffusion experiments identified suitable crystallisation conditions for native protein from the PACT screen (QIAGEN), with optimisation identifying the best conditions for crystal formation as 6 mg/mL protein combined 1:1 with 8% PEG 1500 ...
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bioRxiv - Biochemistry 2020Quote: Initial hits of Nic96186-839-VHH-SAN12 were obtained at 18 °C in 1 day in a 96-well sitting drop tray with a reservoir containing 8% (w/v) PEG 8,000 and 0.1 M tri-sodium citrate pH 5.0 (Protein Complex suite, Qiagen). Hanging drops of 1 ml protein at 6mg/ml and 1 ml of precipitant (7-10% (w/v ...
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bioRxiv - Synthetic Biology 2021Quote: ... and His-tagged protein was purified from cell lysate using a Ni-NTA spin column (Qiagen, 31014). The resulting product was run on a 12% Bis-Tris PAGE gel (Thermofisher ...
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bioRxiv - Molecular Biology 2020Quote: Nme2Cas9 protein was expressed in Rosetta (DE3) cells and purified using a Ni2+-NTA agarose column (QIAGEN) as previously described24 ...
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bioRxiv - Cell Biology 2022Quote: ... 0.2 mM TCEP) the expressed protein was extracted from the supernatant using NiNTA resin (QIAGEN, Germantown, MD). The resulting eluate from the NiNTA resin using lysis buffer supplemented with 250 mM Imidazole pH 7.4 ...
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bioRxiv - Microbiology 2022Quote: ... 6xHis tagged protein were purified by immobilized metal ion chromatography using nickel-NTA super flow resin (Qiagen) as previously described with slight modifications46 ...
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bioRxiv - Plant Biology 2021Quote: Alignments of histone DNA and protein sequences was performed using CLC Main Workbench software version 8.1 (QIAGEN). Predictions of intrinsic disorder were undertaken by PONDR (58) ...
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bioRxiv - Immunology 2022Quote: ... https://www.beiresources.org/).Protein was purified using gravity flow purification with Ni-nitrilotriacetic acid (NTA) agarose (Qiagen, Germany) and concentrated and buffer exchanged in Amicon centrifugal units (EMD Millipore ...
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bioRxiv - Immunology 2019Quote: His-tag purified recombinant proteins were expressed in E.coli and purified by Ni+-NTA-agarose column (Qiagen). Then ...
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bioRxiv - Biochemistry 2019Quote: ... Expressed protein was purified by elution from a nickel-nitrilotriacetic acid agarose (Ni-NTA) (Qiagen, Germantown, MD) column with 0.5 M imidazole and buffer exchanged into TEV buffer (25 mM NaHPO4 ...
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bioRxiv - Genomics 2021Quote: ... RNA-protein complexes were then incubated with Ni-NTA sepharose (Qiagen, 100 μl of slurry per sample) overnight at 4°C and extensively washed ...
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bioRxiv - Developmental Biology 2020Quote: ... The significantly changed proteins were used as inputs for Ingenuity Pathway Analysis (IPA, Version 03-13, Qiagen). The IPA predicted the altered pathways ...
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bioRxiv - Biochemistry 2021Quote: ... All the protein samples were expressed in BL21(DE3) and purified using Ni-NTA Sepharose column (QIAGEN) and Superdex 200 16/600 column (GE Healthcare).
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bioRxiv - Biochemistry 2021Quote: ... The polyhistidine-tagged recombinant proteins were then purified from bacterial lysates with Ni2+-NTA agarose beads (QIAGEN) and washed by three different washing buffers (Washing buffer 1 contained 20 mM Tris-HCl ...