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Citations for Qiagen :
2101 - 2150 of 10000+ citations for Mouse WAP kazal immunoglobulin kunitz and NTR domain containing protein 2 WFIKKN2 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2022Quote: ... Intestinal pieces were wash with PBS and all organs were transferred in Eppendorf tubes containing 500μl of PBS and a 5mm stainless steel bead (Qiagen) and h C57BL/6J mice omogenized using the Qiagen TissueLyser II ...
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bioRxiv - Molecular Biology 2022Quote: ... 10,000 cells per sample were sorted into a 96 well plate containing 50 μl RLT plus Lysis Buffer (Qiagen) supplemented with 1% 2-mercaptoethanol and frozen at −80 °C immediately ...
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bioRxiv - Immunology 2019Quote: ... CD103-KLRG1- and CD103+ from LP and CD103+ IELs) were sorted into tubes containing 100μL TCL lysis buffer (Qiagen) supplemented with 1% β-mercaptoethanol ...
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bioRxiv - Zoology 2020Quote: ... Two independent replicates were performed in 8 μl of volume containing 0.5 U of Hot Start Taq polymerase (Qiagen), 0.18 μM of each primer and 0.04 mg of Bovine Serum Albumin Fraction V (Roche) ...
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bioRxiv - Biochemistry 2020Quote: ... Insoluble cell debris was pelleted by centrifugation and the supernatant was loaded on a column containing NiNTA resin (Qiagen) equilibrated in buffer A ...
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bioRxiv - Neuroscience 2021Quote: ... Every second cryosections (35 μm thick) was collected into 1.5 mL eppendorf tubes containing 350 μl RLT Plus Buffer (Qiagen) supplemented with 1% 2-mercaptoethanol ...
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bioRxiv - Microbiology 2020Quote: ... two multiplex PCRs were carried out in a final volume of 12.5 μL containing 6.25 μL of the Qiagen Multiplex PCR master mix (Qiagen, France), 1 μL of DNA template ...
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bioRxiv - Microbiology 2020Quote: ... Crystals of Brd4(BD1) with YFV-C (1-10) K4ac peptide were grown from a solution containing 0.2 M sodium chloride (QIAGEN), 0.1 M Tris (pH 7.0 ...
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bioRxiv - Cell Biology 2021Quote: ... and transfected with 1 μg of a pcDNA3.1 vector containing human αSyn cDNA (provided by T. Baron, Anses, Lyon, France) using Effecten transfection reagent (Qiagen). Positive clones were selected using geneticin and cultured in Dulbecco’s modified Eagle’s medium (DMEM/F-12 ...
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bioRxiv - Cancer Biology 2020Quote: ... the tissue samples were homogenized in vials containing 1.4 mm ceramic beads (Fisherbrand) and 400 μl RLT buffer (Qiagen) using a bead mill (Bead Ruptor 24 ...
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bioRxiv - Plant Biology 2022Quote: ... Sections were immediately placed into 96-well plates (on ice) containing 10 μL of RLT plus (Qiagen, Hilden, Germany). All instruments and surfaces were cleaned with 80% ethanol ...
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bioRxiv - Molecular Biology 2021Quote: ... Peak fractions containing peptide-coupled ORC were pooled and incubated with 0.3 mL of Ni-NTA Agarose Resin (Qiagen) pre-equilibrated in buffer B with 10 mM imidazole ...
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bioRxiv - Molecular Biology 2021Quote: ... Peak fractions containing peptide-coupled Cdt1 were pooled and incubated with 0.3 mL of Ni-NTA Agarose Resin (Qiagen) pre-equilibrated in buffer D with 10 mM imidazole for 1.5 hr with rotation at 4°C ...
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bioRxiv - Microbiology 2022Quote: Frozen tissue was partially thawed and submerged in lysis buffer containing 1% β-mercaptoethanol and 0.5% Reagent DX (Qiagen) before tissues were homogenised together with TissueRupture (Qiagen) ...
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bioRxiv - Developmental Biology 2022Quote: ... Oocytes and embryos were then washed with D-PBS containing 1 mg/ml polyvinylpyrrolidone (PBS-PVP) and transferred into 50 μl droplets of 0.1% protease (Qiagen) to remove the zona pellucida ...
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bioRxiv - Immunology 2022Quote: ... Single cells from three to four mice per each condition were index-sorted directly into 96-well plates containing 5μl of TCL buffer (Qiagen) supplemented with 1%β-mercaptoethanol using a BD FACS Aria II instrument ...
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bioRxiv - Microbiology 2019Quote: ... The supernatant was removed and transferred into new sterilized 50ml Falcon tubes containing RNA stabilization buffer (Qiagen, Hilden, Germany). The experiments were performed using three biological replicates ...
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bioRxiv - Plant Biology 2019Quote: ... The multiplex TaqMan qPCR reaction was carried out in 25 μl reaction mixture containing 12.5 μl of Rotor-Gene Multiplex PCR Master Mix (Qiagen), 2 μl of the primer mix (5 µmol l−1 each) ...
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bioRxiv - Biochemistry 2020Quote: ... 12 000 rpm 45 min) and the supernatant was loaded onto a column containing NiNTA resin (1 mL, Qiagen) pre-equilibrated with buffer A (10 mL) ...
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bioRxiv - Cell Biology 2020Quote: For both HSC and MK isolation cells were sorted into wells each containing 2.5ul lysis buffer/well: 950uL RLT Plus buffer (Qiagen) and 50ul 20U/ul SUPERase In RNase inhibitor (Ambion ...
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bioRxiv - Physiology 2020Quote: ... Zero Blunt® Topo or pBluescript SKII (+) vectors containing the ArCCKP cDNA were purified (Qiagen Maxiprep, Qiagen, Manchester, UK) and 5 μg of the vector was linearized using restriction enzymes (NEB ...
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bioRxiv - Physiology 2020Quote: ... Zero Blunt® Topo or pBluescript SKII (+) vectors containing the ArCCKP cDNA were purified (Qiagen Maxiprep, Qiagen, Manchester, UK) and 5 μg of the vector was linearized using restriction enzymes (NEB ...
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bioRxiv - Microbiology 2021Quote: ... while pea-sized tissue samples were placed in 1.5ml screw-top cryotubes containing 500ul of either RNA later or lysis buffer (Qiagen), or without medium ...
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bioRxiv - Evolutionary Biology 2019Quote: ... PCR amplification was performed in a total volume of 25 µl containing 12.5 µl of Taq PCR Master Mix (Qiagen), 2.5 µl of each primer (10 mM ...
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bioRxiv - Genomics 2019Quote: ... each cDNA amplification reaction was treated with 1µl uracil cleavage mix containing 0.5 µl Uracil DNA Glycosylase (Enzymatics G5010-1140) and 0.5 µl Elution Buffer (Qiagen 19086).
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bioRxiv - Immunology 2021Quote: ... viable in vitro differentiated CD4+ T cells were sorted into 96-well plates containing 5 μl TCL Buffer (QIAGEN) with 1% 2-mercaptoethanol ...
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bioRxiv - Molecular Biology 2020Quote: ... cells were washed with PBS and the pellet was resuspended in 1 ml PBS containing RNAse A (Qiagen, 19101) at a final concentration of 0.2 mg/ml ...
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bioRxiv - Molecular Biology 2023Quote: ... and sorted into wells of a 96-well plate (185 nuclei/well) containing reverse cross-linking buffer (11µL EB buffer (Qiagen) 0.5µL Proteinase K (Roche) ...
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bioRxiv - Microbiology 2022Quote: ... and then homogenized tissues in 1 ml PBS containing a penicillin/streptomycin mixture by using a TissueLyser II (Qiagen) at 30-Hz oscillation frequency for 3 min ...
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bioRxiv - Neuroscience 2023Quote: ... The filtrated supernatant containing His-thioredoxin-ApoE was applied to a 5 ml Ni-NTA Superflow Cartridge (Qiagen, Germany) pre-equilibrated with TBS buffer A ...
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bioRxiv - Immunology 2023Quote: ... 14-666-315) containing 1 mL sterile PBS and one 5 mm stainless steel bead (QIAGEN Cat. No. 69989). Nucleic acids were isolated from excised granulomas by homogenizing in TRIzol (Invitrogen ...
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bioRxiv - Molecular Biology 2022Quote: ... 1 mM DTT) in tubes containing glass beads (200 mg, 0.4 mm) and were lysed using a TissueLyser LT (Qiagen). To minimize protein degradation ...
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bioRxiv - Microbiology 2023Quote: ... 50000 x g) and the supernatant was filtered and loaded on a column containing Ni2_NTA (nitrilotriacetic) agarose (Qiagen, Netherlands). The column was equilibrated with wash buffer and the bound protein washed ...
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bioRxiv - Immunology 2022Quote: ... 2 μL of cDNA was added to 19 μL first round PCR reaction mix containing 0.5 U HotStarTaq Polymerase (Qiagen), 0.18 μM of each 3’ reverse primer ...
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bioRxiv - Developmental Biology 2023Quote: Blastocysts were washed with DPBS containing 1 mg/ml polyvinylpyrrolidone (PBS-PVP) and transferred into 50 μl droplets of 0.1% protease (Qiagen) to remove the zona pellucida ...
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bioRxiv - Biochemistry 2023Quote: Total RNA was extracted with TRIzol and the upper phase containing RNA was loaded into RNeasy columns (Qiagen, #74004) after the addition of isopropanol ...
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bioRxiv - Bioengineering 2023Quote: ... Culture broths were taken and transferred into each tube containing two volumes of RNAprotect Bacteria Reagent (Qiagen, Hilden, Germany). Samples were pelleted as manufacturer’s instructions and stored at −80°C freezer until extraction ...
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bioRxiv - Biochemistry 2023Quote: ... The filtrated supernatant containing His-tagged NanoLuc was applied to a 5-mL Ni-NTA Superflow Cartridge (Qiagen, Germany) pre-equilibrated with TBS buffer A ...
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bioRxiv - Microbiology 2024Quote: ... The amplification was performed in a 25 μl reaction volume containing 12.5 μl of Rotor-Gene Multiplex PCR Master Mix (Qiagen), 2 μl of the primer mix (5 µM each primer concentration ...
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bioRxiv - Microbiology 2024Quote: ... The combined tissues were submerged in 600μl of lysis buffer containing 30 μl of foaming reagent (Reagent DX, Qiagen) and 60μl of β-mercaptoethanol (Sigma-Aldrich) ...
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bioRxiv - Neuroscience 2021Quote: Mouse posterior cortex was disrupted for RNA isolation using the TissueLyser (QIAGEN, Hilden, Germany) for 2 x 2 min at 20 Hz as recommended by the manufacturer ...
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bioRxiv - Microbiology 2020Quote: ... qPCR was then performed with specific primers for mouse IFN-α11 (PPM03050B-200, Qiagen), mouse β-actin (PPM02945B-200 ...
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bioRxiv - Immunology 2019Quote: ... RT2 Profiler™ PCR Array Mouse Autophagy was purchased from Qiagen (Catalog # PAMM-084Z). RNA extracted from the brain of pups born to infected dams were analyzed for the expression of autophagy-related genes following the manufacturer’s instruction and as previously described (13).
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bioRxiv - Pathology 2024Quote: Mouse kidney outer cortex tissues were dissected and homogenized in QIAzol (QIAGEN, Germantown, MD). Total RNA samples were extracted using RNeasy Plus Universal Kit (QIAGEN ...
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bioRxiv - Developmental Biology 2021Quote: ... Total RNA isolation was then carried out using RNA extraction Kit (RNeasy Mini Kit, Qiagen). PolyA selection/enrichment was the method adopted for library preparation ...
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bioRxiv - Biochemistry 2019Quote: ... and CHO-K1 cells respectively using a RNA isolation kit (RNeasy Mini Kit) from Qiagen. cDNAs were synthesized from the isolated RNA using a cDNA synthesis kit (Advantage RT-PCR kits ...
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bioRxiv - Microbiology 2020Quote: RNA was extracted from cells using RNA extraction kits (QIAGEN, RNeasy Mini Kit, cat. 74106) following the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2020Quote: Total RNA prepared from frozen plant material using plant RNA purification kit (QIAGEN RNeasy Kit) was used for cDNA synthesis preceded by removal of residual genomic DNA with DNase I kit (Invitrogen ...
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bioRxiv - Microbiology 2021Quote: ... with the aid of Power Soil kits and DNA Tissue-Blood kits (QIAGEN, Hilden Germany). The robustness of the DNA was visually monitored using 1.0% agarose gel electrophoresis and quantified using a Qubit and NanoDrop ...
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bioRxiv - Microbiology 2019Quote: ... genomic DNA was extracted from overnight cultures using the kit DNeasy Blood & Tissue Kit (Qiagen) after a lysozyme treatment ...