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Citations for Qiagen :
2351 - 2400 of 10000+ citations for Mouse WAP kazal immunoglobulin kunitz and NTR domain containing protein 2 WFIKKN2 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2023Quote: ... the proteins were purified by Ni-NTA affinity chromatography using Ni-NTA agarose beads (Qiagen) and gel filtration with a HiLoad 16/600 Superdex 75 pg column in buffers containing 20 mM HEPES pH 7.4 ...
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bioRxiv - Biophysics 2024Quote: 6xHis-SUMO-Cys-GAF-DBD protein was first purified on Ni-NTA agarose (Qiagen 30210). Briefly ...
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bioRxiv - Biochemistry 2024Quote: ... The protein was purified by incubation with nickel-nitriloacetic acid (Ni-NTA) agarose resin (Qiagen) for 3h at 4 °C ...
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bioRxiv - Biophysics 2024Quote: ... The recombinant proteins were purified from the supernatants using Ni-NTA agarose affinity columns (Qiagen). The protein concentration was measured using the Bradford method (protein assay kit ...
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bioRxiv - Genetics 2024Quote: ... Proteins were harvested 60 h after infection and purified with Ni-NTA Superflow resin (Qiagen), anion-exchange chromatography (Source 15Q ...
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bioRxiv - Biophysics 2021Quote: ... The supernatant was collected and subjected to affinity purification using 5 mL Hi-Trap column containing Ni-NTA resin (Qiagen) on an AKTA Pure 25L protein purification system (GE Healthcare) ...
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bioRxiv - Biochemistry 2021Quote: ... and cDNA products containing the R2R adapter attached to their 5′ end were cleaned-up by using a MinElute column (Qiagen) to remove unused primer ...
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bioRxiv - Biochemistry 2021Quote: ... Cells with lysis buffer were pulverized in a 10 mL jar containing a 12 mm grinding ball using a TissueLyser II (QIAGEN) by performing 5 rounds of 3 min at 15 Hz ...
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bioRxiv - Immunology 2019Quote: ... mucosal scrapings and feces were homogenized in a 2 ml eppendorf containing 1 ml of DNA extraction buffer and a single 5 mm steel bead in a TissueLyzer (Qiagen) for 3 min at 30 Hz ...
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bioRxiv - Genomics 2019Quote: ... We used a 100 µm nozzle to sort single cells into 96-well plates containing 5 µl TCL buffer (Qiagen) with 1% beta-mercaptoethanol for Smart-seq2 and 384-well plates containing 0.6 µl 1% NP40 (Thermo Fisher Scientific ...
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bioRxiv - Systems Biology 2020Quote: ... followed by flow sorting with a Sony SH800Z (gating on calcein NVOC fluorescence levels) into individual wells of a 96-well plate containing 5 μL of Buffer RLT (Qiagen) and 1% β-mercaptoethanol.
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bioRxiv - Neuroscience 2020Quote: ... 10 to 20 mg of frozen tissue was dissociated using 400 μl of RLT Plus in a 2mL extraction tube containing a 5 mm diameter beads (Qiagen) and agitated at 30 HZ for twice 2 minutes in the TissueLyser II (Qiagen) ...
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bioRxiv - Microbiology 2020Quote: ... frozen tissue was partially thawed and submerged in RLT lysis buffer containing 1% ß-mercaptoethanol and 0.5% reagent DX before being homogenized using a hand-operated TissueRupture (Qiagen). To pool samples in equal proportions ...
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bioRxiv - Microbiology 2020Quote: ... frozen tissue was partially thawed and submerged in lysis buffer containing 1% ß-mercaptoethanol and 0.5% Reagent DX before tissues were homogenized together with TissueRupture (Qiagen). The homogenate was centrifuged to remove any potential tissue residues ...
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Pou3f1 orchestrates a gene regulatory network controlling contralateral retinogeniculate projectionsbioRxiv - Developmental Biology 2022Quote: ... Sorted ßGal+ or GFP+ cells were collected in 1.5ml DNA LoBind® tubes placed at 4°C containing 400µl RLT buffer (Qiagen, 79216) with 1% β-Mercaptoethanol (Millipore Sigma ...
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bioRxiv - Cell Biology 2022Quote: Pellets were thawed and lysed on ice in 15 mL lysis buffer containing freshly added lysozyme and benzonase prepared according to manufacturer instructions (Qiagen Qproteome Bacterial Protein Prep Kit ...
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bioRxiv - Developmental Biology 2022Quote: Whole organoids were washed once with 1X PBS and placed into 2ml microcentrifuge tubes containing one 5mm steel bead (Qiagen) and 1ml of Trizol reagent (ThermoFisher) ...
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bioRxiv - Cell Biology 2022Quote: The plasmid #TH8-77 was digested by Not I and DNA fragment containing cdc8 gene fused with mNeongreen and a 40 aa linker were purified by gel extraction (Qiagen). Obtained DNA fragment was used for the transformation of MBY101 strain (h- ...
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bioRxiv - Immunology 2020Quote: Single cells were sorted using a FACS Aria into 96-well plates containing 5μL of lysis buffer (TCL buffer, Qiagen 1031576) supplemented with 1% β-mercaptoethanol ...
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bioRxiv - Microbiology 2021Quote: ... placed in 250 μL PBS containing a 5 mm stainless steel bead and homogenized using a tissue lyser (Tissue-Lyser II, Qiagen) for 2-minutes at 30 Hz ...
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bioRxiv - Microbiology 2020Quote: ... cDNA MERS-D1 (91) containing basepairs 12259–15470 of the MERS-CoV genome was digested with BgII and purified by Qiagen QIAquick PCR Purification Kit to be used as a standard in the RT-PCR reaction ...
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bioRxiv - Molecular Biology 2021Quote: ... For tissue disruption frozen biopsy samples were transferred to ceramic bead-filled tubes (#KT03961-1-102.BK, Bertin Technologies, Montigny-le-Bretonneux, France) containing 700 µl QIAazol Lysis reagent (#79306, Qiagen) and homogenized for 2x 20 sec at 5000 rpm using a Precellys 24 tissue homogenizer (Bertin Technologies).
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bioRxiv - Microbiology 2021Quote: ... Frozen tissue was partially thawed and submerged in lysis buffer containing 1% ß-mercaptoethanol and 0.5% Reagent DX before tissues were homogenized together with TissueRupture (Qiagen). The homogenate was centrifuged to remove any potential tissue residues ...
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bioRxiv - Microbiology 2020Quote: ... and a portion of the tissue (0.08-0.3g) was placed into pre-labeled microcentrifuge tubes containing 5 mm stainless steel beads (Qiagen Inc., CA). Lung samples were homogenized with DMEM + 2% FBS in a TissueLyser (Qiagen Inc. ...
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bioRxiv - Microbiology 2022Quote: ... from the top A horizon were immediately collected for DNA extractions in 15 mL tubes containing 5 mL Lifeguard Soil Preservation Solution (QIAGEN) using chilled soil-processing trays ...
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bioRxiv - Genetics 2020Quote: ... Bacterial cells lysis was then achieved using Pathogen Lysis Tubes (containing beads) and a lysis buffer in the TissueLyser LT instrument (QIAGEN). Lysates were transferred to QIAamp UCP Mini Columns and bound DNA was eluted in 50 μL of elution buffer ...
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bioRxiv - Genomics 2019Quote: ... For the bacteria-specific 341F/805R47 primer pairs a different reaction mixture was used containing 10x Standard Taq Reaction buffer (Qiagen), 2 mM MgCl2 ...
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bioRxiv - Molecular Biology 2019Quote: ... Beads containing long DNA fragments were separated on a magnet and the supernatant containing only small DNA fragments below roughly 800 bp were cleaned up using MinElute PCR purification columns (Qiagen). All libraries were amplified for 12 cycles in total ...
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bioRxiv - Molecular Biology 2021Quote: ... The supernatant containing His-eIF4A1 or His-eIF4A2 was incubated with a 1.5 ml bed volume of Ni-NTA Superflow (Qiagen, 30430) or a 0.75 ml bed volume of Ni-NTA agarose (Qiagen ...
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bioRxiv - Microbiology 2021Quote: ... samples were weighed and homogenized in DMEM containing 10% FBS and 1% antibiotics using 5 mm stainless steel beads (Qiagen) and the TissueLyser II (Qiagen) ...
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bioRxiv - Genomics 2021Quote: ... an amplicon containing a SNP at the Cast allele was amplified by PCR from cDNA using Hot Start Taq (Qiagen) for 38 cycles ...
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bioRxiv - Genomics 2021Quote: ... were sorted into individual wells of a 96 well plate containing lysis buffer 2.5μL RLT Plus Lysis Buffer (QIAGEN, catalog # 1053393) with 1U/μL SUPERase-In (ThermoFisher ...
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bioRxiv - Genomics 2020Quote: ... 25 nuclei were sorted into each well of 96-well plates (8-10 plates per experiment) containing 12 µl of nuclear lysis buffer (11 µl of EB buffer (Qiagen) supplemented with 0.5 µl of 100X BSA and 0.5 µl of 1% SDS) ...
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bioRxiv - Immunology 2020Quote: ... Supernatants were removed and the remaining cell pellet (500 μl) was transferred into a 15 ml tube containing 2.5 ml RNAprotect® Cell Reagent (Qiagen). The cells were resuspended by vortexing ...
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bioRxiv - Microbiology 2020Quote: ... The DNA template for standard curves was prepared by PCR performed in 50 μl reactions containing 25 μl Taq PCR Mastermix (Qiagen), 19 μl PCR-certified water ...
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bioRxiv - Immunology 2020Quote: ... in 96 flat bottom plates in the presence or absence of PMA (200ng/ml) and ionomycin (300ng/ml) were lysed in 100Sµl lysis buffer containing TCL buffer (Qiagen, 1031576) containing 1% β-mercaptoethanol ...
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bioRxiv - Microbiology 2021Quote: The resulting sequencing data was analyzed by mapping reads to a database containing 60 bases of sequence flanking each secondary gene using CLC Genomics Workbench (Qiagen). Total read counts at each locus were normalized to the secondary gene with the greatest number of reads in each sample ...
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bioRxiv - Immunology 2021Quote: Single cells were index-sorted using a FACS Aria into 96-well plates containing 5μL of lysis buffer (TCL buffer, QIAGEN 1031576) supplemented with 1% β-mercaptoethanol ...
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bioRxiv - Developmental Biology 2022Quote: ... Stable cell lines were obtained by transfection with pMT puro vectors containing either Zasp52-PF or Zasp52-PF-ABM using Effectene (Qiagen) according to the manufacturer’s instructions ...
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bioRxiv - Genetics 2022Quote: ... an amplicon containing a SNP at the Cast allele was amplified by PCR from cDNA using Hot Start Taq (Qiagen) for 38 cycles ...
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bioRxiv - Microbiology 2022Quote: ... pH 8.0) containing 1 mg/mL of lysozyme and supplemented with 10 μL of Proteinase K (QIAGEN, cat. no. 19131). The mixture was incubated on a shaker at room temperature for 30 min with intermittent vortexing ...
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bioRxiv - Microbiology 2022Quote: ... Left lungs were harvested in 2 ml Eppendorf (Stevenage, UK) Safe-Lock micro test tubes containing 500 µl DMEM and a stainless-steel carbide bead (Qiagen), and snap frozen on dry ice until virus titration ...
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bioRxiv - Genetics 2024Quote: ... they were transferred to Eppendorf tubes containing either 180 µL buffer ATL and 20 µL Proteinase K (Qiagen, Hilden, Germany), or 350 µL of in-house ZB5d medium/antibiotics mixture as described in Ries et al ...
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bioRxiv - Microbiology 2024Quote: ... Bacteria are harvested by centrifugation (5 minutes, 4°C) and washed with 1 ml cold PBS containing RNA stabilization reagents (RNAprotect, Qiagen) before flash freezing and storage at -80°C ...
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bioRxiv - Microbiology 2023Quote: ... Cells were sorted into 96-well plates containing lysis buffer with proteinase K solution in PKD buffer (1:16) (Qiagen) and were stored at -80ºC ...
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bioRxiv - Cell Biology 2023Quote: ... Twenty-four hours later 100,000 cells were transferred to a tube containing 100 µl of RLT buffer (Qiagen, Hilden, Germany). Cells were stored at –80 °C until further processing ...
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bioRxiv - Microbiology 2023Quote: ... The cleared lysate was applied to an affinity chromatog-raphy column containing Ni-nitrilotriacetic acid (NTA) su-perflow resin (Qiagen) pre-equilibrated in lysis buffer ...
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bioRxiv - Biophysics 2023Quote: ... beads were discarded and the supernatant containing reconstituted into ND A2AAR was incubated for 4 h with 250 µL of Ni-NTA resin (Qiagen) for separating from empty ND ...
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bioRxiv - Microbiology 2023Quote: ... a single GFP+ or GFP-J-Lat 11.1 cell was sorted directly into each well of a 96-well PCR plate containing the pre-amplification mastermix consisting of 1 μL One-step RT-PCR enzyme (QIAGEN), 5 μL 5× One-step RT-PCR buffer (QIAGEN) ...
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bioRxiv - Genomics 2022Quote: ... We discarded the flowthrough of the PVDF membrane and transferred the membrane immediately after filtration to a 0.7 mm Garnet particle containing tube (13123-50, MoBio – now QIAGEN PowerBead) using sterile tweezers ...