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Citations for Qiagen :
2101 - 2150 of 2779 citations for 4 2 Hydroxy 1 Methoxyethyl 1 2 Benzenediol since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genomics 2022Quote: ... where IPA-defined core analyses for Canonical Pathways and Disease and Biological functions were performed using all primary cell subsets (Figure 1) (82) (QIAGEN Inc. ...
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bioRxiv - Microbiology 2022Quote: ... were inoculated in 20 mL tryptone soy broth and incubated at 37°C for 24 h before 1 mL was pelleted and DNA extracted using the DNeasy Blood & Tissue kit (Qiagen). Libraries for genome sequencing were prepared using the NEBNext Ultra DNA Library Prep Kit (New England Biolabs ...
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bioRxiv - Cell Biology 2022Quote: ... Drosophila S2 cells were transfected with mNeonGreen-tagged kinesin-1 full-length heavy chain (KHC) and Halo-tagged PLP584-1811 (Effectene, QIAGEN). The lysates were harvested for TIRF motility assays at ∼48 h post-transfection ...
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bioRxiv - Biochemistry 2022Quote: ... The Klenow polymerization reactions were incubated at 37 °C for 1 h and then purified on ion-exchange cartridges (cat. no. 28306; Qiagen). The 194 purified reactions were dissolved in 50 µL of water each and pooled to generate the 3,880 members library (DEL3880 ...
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bioRxiv - Cancer Biology 2022Quote: Spheroid and monolayer samples (technical replicates n=3) of MUC-1 and NCI-H295R cells were processed for RNA extraction using the RNeasy Mini kit (Qiagen), followed by DNA removal (TURBO DNA-free™ Kit ...
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bioRxiv - Genomics 2022Quote: PCR amplicons verified by 1% agarose gel electrophoresis were purified from gel using Gel extraction kit (Qiagen GmbH, Hilden, Germany) and ligated into a pGEM-T easy cloning vector (Promega ...
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bioRxiv - Neuroscience 2020Quote: ... frozen tissue (hippocampus or cortex) from the left hemisphere were put into 1 ml QIAzol Lysis reagent (Qiagen, Hilden, Germany) and immediately homogenized by a rotor-stator homogenizer (TissueRuptor ...
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bioRxiv - Developmental Biology 2021Quote: ... RNA was extracted from pooled (n=10) embryonic zebrafish and pooled (n=3) adult (> 1 year) heart samples by QIAGEN RNEasy Lipid Tissue Extraction kit according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2021Quote: ... For tissue disruption frozen biopsy samples were transferred to ceramic bead-filled tubes (#KT03961-1-102.BK, Bertin Technologies, Montigny-le-Bretonneux, France) containing 700 µl QIAazol Lysis reagent (#79306, Qiagen) and homogenized for 2x 20 sec at 5000 rpm using a Precellys 24 tissue homogenizer (Bertin Technologies).
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bioRxiv - Microbiology 2021Quote: ... Frozen tissue was partially thawed and submerged in lysis buffer containing 1% ß-mercaptoethanol and 0.5% Reagent DX before tissues were homogenized together with TissueRupture (Qiagen). The homogenate was centrifuged to remove any potential tissue residues ...
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bioRxiv - Neuroscience 2021Quote: ... cDNA was generated from CSF isolated RNA and a control RNA sample spiked with miR-39 (1 × 108 copies/ul) using the miScript HiSpec kit (Qiagen); dilutions were created from the control cDNA sample spiked with miR-39 (1 ×106-1×103 copies ...
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bioRxiv - Microbiology 2021Quote: ... (1 μl) for 18 h and the RNA was extracted by using the mirCury cell and plant isolation kit (QIAGEN). A final number of 108 treated cells were lysed using 500 μL of glass beads and the 600 μL of the Lysis buffer provided with the kit ...
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bioRxiv - Microbiology 2021Quote: ... PCR amplifications were carried out in 50-μL total volumes containing 1 μL of template using a DNA HotStarTaq Master Mix kit (Qiagen). The cycling protocol was as follows ...
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bioRxiv - Microbiology 2020Quote: ... MB897-1) were used to generate infectious virus by transfecting 293T cells using the Effectene transfection reagent (QIAGEN, Valencia, CA). Supernatant containing virus was collected 2 days post transfection ...
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bioRxiv - Plant Biology 2021Quote: ... and sesame (cv. Kanto-1) using an RNeasy Plant Mini Kit™ according to the manufacturer’s instructions (Qiagen, https://www.qiagen.com). Reverse transcription of RNAs were carried out using PrimeScript II 1st strand cDNA Kit™ (Takara ...
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bioRxiv - Cell Biology 2021Quote: ... The extracellular RNAs from 1 mL CPB patient plasma samples were isolated using the exoRNeasy Midi kit (Qiagen, Cat# 77044).
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bioRxiv - Microbiology 2020Quote: ... viral RNA was extracted from either DF-1 cultures infected with the WT virus or DF-1P cells using the Qiamp viral RNA mini kit (Qiagen) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2020Quote: ... 0.2 μM gene specific primers pairs (hVDAC1, hVDAC2, hVDAC3, β-actin (Table 1) and 12.5 μl of master mix (QuantiFast SYBR Green PCR kit, Qiagen). Three independent experiments of quantitative real-time were performed in triplicate for each sample ...
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bioRxiv - Microbiology 2021Quote: ... The digested DNA was separated on a 1% agarose gel and the ∼5 Kb genomic DNA band was harvested with a QIAquick Gel Extraction Kit (Qiagen). The DNA library was prepared according to the manual of the Ligation Sequencing Kit (Nanopore) ...
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bioRxiv - Microbiology 2022Quote: ... loops were taken out from the re-opened cavity and each loop was homogenized in 1 ml sterile PBS using TissueLyser LT (Qiagen) and stainless-steel beed ...
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bioRxiv - Pathology 2022Quote: ... and 500 ng to 1 μg of DNA was treated with bisulfite using an EpiTect DNA bisulfite kit (QIAGEN, Germany) according to the manufacturer’s guidelines ...
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bioRxiv - Cancer Biology 2022Quote: ... shRNA expressing cells were isolated by fluorescence activated cell sorting at passage doubling 1 and 15 followed by genomic DNA isolation with Gentra Puregene cell kit (Qiagen). Sequencing libraries were prepared by subjecting 18 μg (1.2 μg x 15 reactions ...
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bioRxiv - Biochemistry 2022Quote: ... as previously described.10 Detection of 6x His-tagged recombinant POLIB variants was performed with primary antibody Penta•His (1:1000, Qiagen) for one hour ...
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bioRxiv - Biophysics 2022Quote: ... Insoluble material was pelleted for 1 hr at 100,000 x g and the supernatant was added to Ni-NTA resin (QIAGEN, 30410) in batch and was eluted with an imidazole gradient containing 50 mM Tris-HCl pH 7.5 ...
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bioRxiv - Immunology 2020Quote: Genomic DNA was extracted from 1×10^7 PBMCs using the AllPrep DNA/RNA/miRNA Universal Kit (Qiagen, Hilden, Germany). Following nucleic acid isolation ...
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bioRxiv - Microbiology 2020Quote: ... Cells were lysed by boiling (5 min 95°C) followed by bead beating (3mm beads, 30 Hz for 1 min) (TissueLyser II, Qiagen) and sonication bath (3×10 sec at 4°C ...
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bioRxiv - Microbiology 2020Quote: ... and positive swabs at 3 days post infection (d.p.i.) with a Cq value < 30 (Table 1) using QIAamp viral RNA Mini kit (Qiagen, Hilden, Germany) according to the kit protocol ...
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bioRxiv - Developmental Biology 2020Quote: ... DNA was finally purified by reverse crosslinking at 65°C overnight followed by proteinase K (0.5 or 1 mg/ml, respectively) digestions and purifying with MinElute® PCR purification kit (Qiagen).
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bioRxiv - Genomics 2020Quote: 3 million cells from cell lines or patient samples were pelleted and resuspended in 1 mL of Cell Lysis Solution (Qiagen) mixed with 500 μg of RNase A ...
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bioRxiv - Developmental Biology 2021Quote: ... Remote biopsies were taken using an air-powered rifle affixed with a 1 mm wide by 20-25 mm deep biopsy dart and preserved in RNAlater (Qiagen). Blood samples were collected opportunistically during veterinarian visits ...
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bioRxiv - Cell Biology 2021Quote: ... First-strand complementary DNA synthesis was performed using 1 µg of total RNA with an anchored oligo (dT) and Transcriptor Reverse Transcriptase (QIAGEN) following the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2020Quote: ... Purification of p53-(1-73) from the clarified cell lysate was accomplished using a Nickel Nitrilotriacetic Acid (Ni-NTA, Qiagen) column purification and 50 mM Tris-HCl ...
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bioRxiv - Molecular Biology 2020Quote: DNA extraction was done from 1 ml of the different sample types using QIAamp DNA mini kit (Qiagen, Hilden, Germany), as per the vendor’s instructions with slight modifications ...
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bioRxiv - Molecular Biology 2020Quote: ... catalysis was measured using a previously described assay (22) in which hexahistidine-tagged PARP-1 was immobilized on Ni(II)-NTA-coated plates (Qiagen) in the presence of NAD+/biotinylated-NAD+ (Trevigen) ...
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bioRxiv - Microbiology 2020Quote: ... HIV-1 integration was examined in 293T cells and J-Lat cells by isolating genomic DNA from 1 × 106 cells using the DNeasy Tissue Kit (Qiagen). Alu-LTR sequences were amplified during first round PCR from 100 ng of total genomic DNA using the following primers ...
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bioRxiv - Evolutionary Biology 2020Quote: ... several loci were amplified simultaneously from 1 μl of extracted DNA using 5 μl of the Multiplex PCR Master Mix (Qiagen) and varying amounts of the pooled primer mixes (Pool A ...
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bioRxiv - Physiology 2021Quote: ... Shredded bone marrow cells were frozen (−80°C) in RLT buffer (1% beta-mercaptoethanol) until RNA extraction using RNAeasy mini kit (Qiagen). RNA integrity number (RIN ...
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bioRxiv - Immunology 2020Quote: ... mRNAs recovered from microdissected samples or vaginal biopsies (about 1 mm3) were reverse-transcribed with the QuantiTect Rev Transcription Kit (Qiagen), used according to the manufacturer’s recommendations ...
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bioRxiv - Microbiology 2020Quote: ... Glass beads (3mm) and 1 mL of sterile water were added for homogenization at 30Hz for 10 minutes (TissueLyser II, Qiagen). Homogenates were diluted with PBS and inoculated onto SAB plates and incubated for 3 days at 30°C ...
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bioRxiv - Microbiology 2020Quote: ... Preparation of total RNA and Northern blotting including the determination of RNA half-lives were carried out as described previously [29] except that 1 ml time samples were taken and directly added to 250 μl RNAprotect Bacteria Reagent (Qiagen) or to 250 μl containing 5 % phenol and 95 % ethanol ...
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bioRxiv - Microbiology 2020Quote: ... The PCR fragment was then digested with Dpn I at 37 °C for 1 h and purified using the QIAquick PCR purification kit (Qiagen). To prepare the competent SW102 containing the pSMART BAC-T7-scv2-replicon ...
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bioRxiv - Microbiology 2021Quote: ... samples were weighed and homogenized in DMEM containing 10% FBS and 1% antibiotics using 5 mm stainless steel beads (Qiagen) and the TissueLyser II (Qiagen) ...
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bioRxiv - Bioengineering 2021Quote: ... The plasmid coding for FLP recombinase and the mCherry reporter or 47bp-insertion TLR reporter plasmid were transfected into the cells at a 9:1 ratio using polyfect (QIAGEN) according to the manufacturer’s protocol (900ng mCherry reporter or 47bp-insertion TLR reporter plasmid and 100ng FLP recombinase plasmid to make 1 μg plasmid in total) ...
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Glutaric aciduria type 3 is a naturally occurring biochemical trait in inbred mice of 129 substrainsbioRxiv - Biochemistry 2020Quote: ... were homogenized in ice-cold RIPA buffer supplemented with protease inhibitor cocktail (∼20 mg tissue per 1 mL lysis buffer) using a TissueLyser II apparatus (Qiagen) and the homogenates were centrifuged at 1,000xg ...
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bioRxiv - Animal Behavior and Cognition 2020Quote: ... we transfected S2 cells in each well of a 6-well plate with 1 µg each of HA/GFP-tagged constructs using Effectene (Qiagen). After incubation for 3 days ...
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bioRxiv - Bioengineering 2021Quote: ... for 1 day prior to extraction of total RNA of the treated leaves with an RNeasy plant mini kit (QIAGEN). Two-step qPCR was performed to quantify GFP gene silencing using the total RNA extracted from leaves ...
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bioRxiv - Cell Biology 2021Quote: ... The remaining lysate was transferred to a 15 ml falcon tube and 1 ml of a 50% slurry consisting of NiNTA agarose beads (Qiagen) in resuspension buffer was added per 10 ml of lysate ...
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bioRxiv - Genomics 2021Quote: ... The PCR products were size-selected on a 1% agarose gel and purified using the MinElute PCR Purification Kit (Qiagen). The pSTARR-seq_human vector (kindly provided by Alexander Stark ...
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bioRxiv - Genomics 2021Quote: ... This was followed by RNase A treatment for 1 h at 37°C and purification using MinElute PCR purification columns (Qiagen). cDNA amplification was performed with reporter-specific nested cDNA primers (Oligos 16 and 17 for libraries in vectors d and e ...
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bioRxiv - Immunology 2021Quote: ... Complementary deoxyribonucleic acid (cDNA) was synthesised from every 1 μg of total mRNA in 20 μL volume with QuantiTect Reverse Transcription Kit (Qiagen) according to the manufacture protocol ...