Labshake search
Citations for Qiagen :
2001 - 2037 of 2037 citations for L β Homo β 2 hydroxyphenyl glycine; R 3 Amino 3 2 hydroxyphenyl propanoic acid since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Neuroscience 2021Quote: ... Hsa-miR-124-3p Locked Nucleic Acid (LNA) power inhibitor and has-miR-124- 3p miRCURY LNA miRNA mimic (Qiagen, Germantown, MD) were resuspended with RNase-free water to 100 μM ...
-
bioRxiv - Biochemistry 2020Quote: ... A final 370,000 x g centrifugation at 4°C for 45 min yielded a myosin-containing supernatant that was loaded onto a 1 mL nickel-nitrilotriacetic acid agarose (Ni-NTA) (Qiagen, Cat# 30210) column equilibrated with column buffer (50 mM Tris-HCl pH 8.0 ...
-
bioRxiv - Systems Biology 2021Quote: Approximately 10 mL of amniotic fluid from each animal was used for cell-free RNA extraction with the QIAamp Circulating Nucleic Acid Kit (QIAGEN, Valencia, CA). RNA from the lung and hippocampus was extracted with the RNeasy Universal Mini Kit (QIAGEN ...
-
Cell-free DNA as a biomarker for prostate cancer: elevated concentration and decreased fragment sizebioRxiv - Cancer Biology 2020Quote: ... DNA was extracted from 7 to 55 mL of plasma using the Qiagen QIAamp Circulating Nucleic Acid Kit (Qiagen, Redwood City, CA), and double eluted with 40 μL of Qiagen Elution Buffer ...
-
bioRxiv - Biochemistry 2020Quote: ... at 4°C and the supernatant was loaded onto a 1 ml nickel nitrilotriacetic acid-agarose column (Ni-NTA, Qiagen, Hilden, Germany) previously equilibrated with the corresponding lysis buffer ...
-
bioRxiv - Cell Biology 2020Quote: RNA FISH was performed using a FITC-labeled DNA oligo probe complementary to alpha satellite (Integrated DNA Technologies, Newark, NJ) or a biotinylated locked nucleic acid (LNA) oligo probe complementary to HSATII (QIAGEN, Hilden, Germany) (see Key Resources Table) ...
-
bioRxiv - Molecular Biology 2022Quote: ... hirae V-ATPase was expressed recombinantly in Escherichia coli and purified by affinity purification with a Ni+-NTA (nitrilotriacetic acid) column (Ni+-NTA Superflow; Qiagen, Hilden, Germany). The column was preconditioned with buffer consisting of 50 mM potassium phosphate ...
-
bioRxiv - Bioengineering 2024Quote: ... the extraction of total nucleic acid from transfected MOLT-4 cells (small scale, as described) was performed using a DNeasy Blood and Tissue Kit (QIAgen catalog # 69504) which includes direct lysis with proteinase K treatment ...
-
bioRxiv - Microbiology 2024Quote: ... The clarified lysate was transferred into the glovebox and loaded onto a gravity column in with 1 mL nickel-nitrilotriacetic acid resin (Qiagen, Hilden, Germany) that was preequilibrated with wash buffer (50 mM Tris-HCl pH 7.5 ...
-
bioRxiv - Microbiology 2024Quote: Total nucleic acid (DNA and RNA) was extracted from BDB by PowerSoil DNA Isolation Kit or PowerSoil Total RNA Isolation Kit (Qiagen, Hilden, Germany). qPCR was carried out for viral and protozoan pathogens including enterovirus ...
-
bioRxiv - Biophysics 2024Quote: ... The clarified lysate was then applied to a 10 mL bed volume Nickel nitrilotriacetic acid (Ni-NTA) agarose (Qiagen, cat. no. 30230) column ...
-
bioRxiv - Genomics 2021Quote: Genomic DNA from MCF7 cells was isolated by proteinase K digestion at 65°C for 30 minutes followed by purification using the QIAamp circulating nucleic acid kit (Qiagen, Venlo, The Netherlands). Genomic DNA from frozen tissue sections of colorectal liver metastases was isolated using the NucleoSpin Tissue kit according to the manufacturer’s guidelines ...
-
bioRxiv - Neuroscience 2020Quote: ... RNA was then purified using a nucleic acid binding column with on-column DNase treatment (RNase-free DNase set, QIAGEN, Germantown, MD, USA). RNA was then eluted from the column in elution buffer.
-
bioRxiv - Genomics 2022Quote: ... Nucleic acids were extracted at CNM using either QIAamp MinElute Virus Spin (DNA) or QIAamp Viral RNA Mini kits (Qiagen, Germantown, MD, USA) according to the manufacturer’s recommendations ...
-
bioRxiv - Bioengineering 2024Quote: ... The clarified supernatant was loaded onto a pre-equilibrated gravity flow column of nickel-nitrilotriacetic acid (Ni-NTA) beads (Qiagen, Germantown, MD, USA). The column was washed with the increasing concentration of imidazole and the recombinant nanobody protein was eluted at a gradient of 250-500 mM imidazole concentration in elution buffer (50 mM Sodium Phosphate ...
-
bioRxiv - Microbiology 2024Quote: ... 140µl of the sample suspension was used to extract total viral nucleic acid using the QIACube QIAamp viral RNA mini kit (Qiagen, Valencia, CA, USA) using the QIACube extraction instrument (Qiagen ...
-
bioRxiv - Biophysics 2024Quote: ... for 30 min at 4 °C before being applied to a 10mL bed volume Nickel nitrilotriacetic acid (Ni-NTA) agarose (Qiagen, cat. no. 30230) column ...
-
bioRxiv - Developmental Biology 2020Quote: ... 100 μl of chloroform was added and mixed vigorously and the aqueous component was isolated using MaXtract High Density tubes (Qiagen, 129046) using the manufacturers protocol ...
-
bioRxiv - Genetics 2021Quote: ... incubated overnight at 37°C in L-broth containing 50 ng/μL ampicillin and DNA isolated using the QIAprep Spin Miniprep kit (Qiagen 27104) according to the manufacturer’s instructions ...
-
bioRxiv - Immunology 2021Quote: ... 3.5 μl of cDNA was used as template and amplified in a total volume of 40 μl with a mix of forward L-VH primers (Table S1) and reverse Cγ primer and using the HotStar® Taq DNA polymerase (Qiagen) and 50 cycles of PCR (94°C 30 s ...
-
bioRxiv - Developmental Biology 2022Quote: ... The resulting 1.5 L bacterial culture was incubated at 37°C for 2h and a Maxiprep performed using Qiagen MaxiPlus kit (Qiagen #12963) to a resulting 1 mg of plasmid DNA ...
-
bioRxiv - Immunology 2023Quote: ... 3.5 μl of cDNA was used as template and amplified in a total volume of 40 μl with a mix of forward L-VH primers (Table S3) and reverse Cγ primer and using the HotStar® Taq DNA polymerase (Qiagen) and 50 cycles of PCR (94°C 30 s ...
-
bioRxiv - Genomics 2024Quote: U2OS cells were subjected to siRNA-mediated knock-down of either CTCF (siCTCF, Dharmacon, L-020165-00-0005) or RING1B (siRNF2, QIAGEN, S100095543). Each siRNA treatment was accompanied by a control (siControl ...
-
bioRxiv - Cell Biology 2024Quote: HeLa or HEK293 cells were grown in Dulbecco’s Modified Eagle Medium (DMEM, low glucose 1 g L−1, Qiagen #31885-023) supplemented with 10% fetal bovine serum (FBS ...
-
bioRxiv - Microbiology 2022Quote: 200 μl of apical wash was harvested at 48 and 72 hpi and 100 μl inactivated in 400 l AVL buffer (Qiagen, Hombrechtikon, Switzerland) for 10 min at RT then 400 μl of absolute ethanol were added to each sample ...
-
bioRxiv - Neuroscience 2020Quote: ... 500 μl ethanol and 500 μl corn oil were added to 50 mg tamoxifen and mixed in a tissue lyser (Qiagen, Hilden, Germany) for 10 min at 50 Hertz ...
-
bioRxiv - Microbiology 2021Quote: ... total genomic DNA was extracted from a culture grown on R2B with 200 mg/L BAM using the DNeasy UltraClean Microbial Kit (Qiagen, Hilden, Germany). Afterwards ...
-
bioRxiv - Microbiology 2020Quote: ... of a 6-well plate in 200 μL ice-cold PBS + 10% Proteinase K and processed through the DNeasy Blood and Tissue Kit following manufacture protocols (QIAGEN, Hilden, Germany). gDNA was stored at −20° C for short-term storage or −80° C for long-term storage.
-
bioRxiv - Molecular Biology 2022Quote: ... An aliquot of cells was diluted to OD600 0.3 and 500 µL of diluted cells was added to 1 mL of RNAprotect Bacteria Reagent provided in the RNeasy Mini Kit (Qiagen; catalog #74104). Protocols provided in the “RNAprotect Bacteria Reagent Handbook 2015” were used for RNA extraction (“Protocol 1”) ...
-
bioRxiv - Evolutionary Biology 2024Quote: ... DNA removal was performed by applying 80 μl of DNase I working solution (10 μL DNase stock + 70 μL 1x RDD buffer; RNase-Free DNase Set, Qiagen, Hilden, Germany) to the column and incubated for 15 min at room temperature ...
-
bioRxiv - Microbiology 2022Quote: ... of a 6-well plate in 200 μL ice-cold PBS + 10% Proteinase K and processed through the DNeasy Blood and Tissue Kit following manufacture protocols (QIAGEN, Hilden, Germany). gDNA was stored at -20° C for short-term storage or -80° C for long-term storage.
-
bioRxiv - Microbiology 2024Quote: ... 450 μl sample was pooled into separate Eppendorf tubes and total RNA was extracted using the RNeasy Protect Mini Kit (Qiagen, catalog# 74124). Isolated RNA samples were quantified using both Qubit dsDNA HS (High Sensitivity ...
-
bioRxiv - Microbiology 2021Quote: ... Nucleic acid was extracted from 200 μl of each sample using the QIAamp MiniElute™ Virus Spin Kit nucleic acid (for DNA and RNA) according to the manufacturer (QIAgen Canada, Toronto, ON, Canada). DNA samples were stored at −80°C and assayed in duplicate by qPCR ...
-
bioRxiv - Microbiology 2023Quote: A 200 L sample of faecal slurry was subjected to automated nucleic acid extraction using the RNeasy PowerMicrobiome kit on the QIAcube device (Qiagen, German-town, MD, United States). With the exception of skipping the phases for DNA degradation ...
-
bioRxiv - Bioengineering 2021Quote: ... 7.5 μL of RNAse free H2O and 10 μL of 2X HotStarTaq™ Plus Master Mix (HotStarTaq™ Plus Master Mix Kit, Qiagen, USA). PCR conditions were an initial denaturation of 5 min at 95°C followed by 25 cycles of 30 s at 95°C ...
-
bioRxiv - Genetics 2024Quote: ... 30 μl clarified lysate were mixed with 70 μl RNase free water and RNA isolated using the RNeasy Mini Kit (Qiagen, Germantown, MA, USA) according to the manufacturer’s recommendations ...
-
bioRxiv - Physiology 2022Quote: ... 300 μl of supernatant was transferred into a new tube for RNA extraction using the miRNeasy® Serum/plasma advanced kit (Qiagen, Venlo, The Netherlands) following the manufacturer’s instructions ...