Labshake search
Citations for Qiagen :
1901 - 1950 of 10000+ citations for Cyclic GMP Direct ELISA Kit 5 Plate since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Plant Biology 2022Quote: All PCR reactions were performed with the 5-Prime Hot Master Mix (Quantabio, QIAGEN, Beverly, MA, U.S.A.). All PCR products and pooled library were purified with CleanNGS beads (CleanNA ...
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bioRxiv - Biophysics 2023Quote: ... The supernatant was added to a 50 mL Falcon tube containing 5 ml Ni-NTA suspension (Qiagen) resuspended in solution A and left to settle on a tilting table for 1 hour at RT ...
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bioRxiv - Biochemistry 2023Quote: ... Plasmid DNA was extracted and purified from 5 ml or 250 ml bacterial culture using Miniprep (Qiagen) or Maxiprep (Thermo-Scientific ...
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bioRxiv - Biophysics 2023Quote: ... The supernatant was added to a 50 mL Falcon tube containing 5 ml Ni-NTA suspension (Qiagen) resuspended in 2 mL solution A and left to settle on a tilting table for 1 hour at RT ...
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bioRxiv - Microbiology 2023Quote: ... NGS libraries were constructed using Enzymatics 5× WGS fragmentation mix and WGS ligase reagents (Qiagen, Hilden, Germany), and then libraries were sequenced on HiSeq X Five platform in Macrogen Japan (Tokyo ...
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bioRxiv - Molecular Biology 2023Quote: ... Supernatant was passed through a 5 mL column volume (CV) of Ni-NTA agarose resin (Qiagen: 30250). The column was then washed with 10 CV of His binding buffer (50 mM NaH2PO4 ...
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bioRxiv - Microbiology 2023Quote: ... 5 ml of the supernatant were added to 1 ml of Nickel-NTA Agarose (Qiagen, Hilden, Germany) and incubated under continuous shaking at 4 °C for 1 h ...
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p110α-dependent hepatocyte signaling is critical for liver gene expression and its rewiring in MASLDbioRxiv - Cell Biology 2024Quote: ... The albumin-Cre allele was detected by PCR and HotStar Taq DNA Polymerase (5 U/μl, Qiagen) using the following primers ...
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bioRxiv - Microbiology 2020Quote: ... to recover parasite material for genomic DNA isolation using a kit (Qiagen DNA Easy Blood and Tissue Kit), according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2021Quote: ... Plasmids and PCR products were purified with QIAprep plasmid Miniprep kit and QiaQuick PCR purification kit respectively (Qiagen). Plasmid Sanger sequencing was performed by Eurofins Genomics ...
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bioRxiv - Evolutionary Biology 2019Quote: ... using a modified Trizol isolation protocol in combination with a column based commercial kit (QIAGEN RNeasy Mini Kit) based on the manufacturer’s protocol ...
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bioRxiv - Physiology 2019Quote: DNA of selected LAB was extracted using DNA extraction kit (QIAamp Blood and Tissue Kit, Qiagen, Hilden, Germany). The amplification of 16SrRNA genes were conducted using 27F 5’-AGAGTTTGATCCTGGCTCAG-3’ and 1492R-5’-GGCTACCTTGTTACGACTT-3’primers ...
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bioRxiv - Microbiology 2021Quote: ... genomic DNA was extracted using the DNAeasy® UltraClean® Microbial kit Isolation Kit (Qiagen Inc., MD, USA) and the near full-length 16S rRNA gene was amplified using primers 8F (5’-AGAGTTTGATCCTGGCTCAG-3’ ...
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Impairment of a distinct cancer-associated fibroblast population limits tumour growth and metastasisbioRxiv - Cancer Biology 2020Quote: RNA was isolated using Qiagen RNeasy kit and cDNA was generated using the QuantiTect reverse transcription kit (Qiagen) according to the manufacturer’s instruction ...
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bioRxiv - Genomics 2020Quote: ... and RNA and DNA were extracted (Qiagen RNEasy Mini Kit #74104/Qiagen DNA Blood and Tissue Kit #69504). Clones were again genotyped to verify absence of the mariner insertion (Fig ...
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bioRxiv - Neuroscience 2021Quote: RNA purification was completed according to the instructions of the manufactured kit (RNeasy® Plus Mini kit; Qiagen). Briefly ...
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bioRxiv - Microbiology 2022Quote: ... against five other extraction kits: the Qiagen MagAttract PowerSoil Pro DNA Isolation Kit (Cat#: 47109; Qiagen, Carlsbad, CA), the Norgen Stool DNA Isolation Kit (Cat# 65600 ...
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bioRxiv - Molecular Biology 2022Quote: ... RT-qPCR reaction was carried out using the QuantiNova Kit Probe RT-PCR Kit (Qiagen, catalog #208354, Germany), using primers and probe described in Naveca et al ...
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bioRxiv - Microbiology 2022Quote: ... The DNA extraction was performed using the PowerSoil DNA extraction kit (DNeasy PowerSoil Pro Kit, QIAGEN, Hilden, Germany), according to the manufacturer’s protocol with an additional 500 μL of 10% (w/v ...
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bioRxiv - Systems Biology 2023Quote: ... total RNA of the samples was extracted according to manufacturing specifications (RNeasy Protect Bacteria Mini Kit Qiagen kit).
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bioRxiv - Genomics 2023Quote: Total RNA from tissues was extracted using a commercial kit (RNeasy Plus Mini Kit; Qiagen, Toronto, ON, Canada). Quality control checks ...
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bioRxiv - Molecular Biology 2023Quote: ... All plasmids were purified with a Midiprep kit (ZymoPURE, #D413) or a QIAprep spin miniprep kit (QIAGEN, #27104) and verified by DNA sequencing prior to use ...
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bioRxiv - Evolutionary Biology 2023Quote: ... The selected BACs were recovered and sequenced on an Illumina MiSeq using reagent kit Index Nextera XT kit V2-500 cycles and assembled with the CLC Genomics Workbench 20.0 v7.5 (QIAGEN). P ...
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bioRxiv - Microbiology 2023Quote: Total DNA and mRNA were isolated using QIAamp DNA mini kit and RNeasy mini kit (Qiagen, Germantown, MD), per the manufacturer’s recommendation ...
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bioRxiv - Molecular Biology 2023Quote: ... Genomic DNA was extracted using the genomic DNA purification kit (Dneasy Blood and Tissue kit, Cat. 69504, Qiagen), the specific fragment containing rG4-1 sequence was amplified using Phire Green Hot Start II PCR Master Mix (F126S ...
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bioRxiv - Genomics 2023Quote: DNA from both types of samples was extracted using a commercially available kit (DNeasy Blood & Tissue Kit, QIAGEN) following the manufacturer’s protocol ...
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bioRxiv - Genomics 2023Quote: ... We used the MagAttract® HMW DNA kit and the DNeasy Blood and Tissue kit (Qiagen, Hilden, Germany) for DNA isolation ...
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bioRxiv - Microbiology 2024Quote: ... DNA extraction was performed utilizing commercial kits and following manufacturer’s protocol (Qiagen’s DNeasy 96 Blood & Tissue Kit, Qiagen). The amount and quality of extracted DNA was evaluated using PicoGreen fluorometer and NanoDrop spectrophotometer (ThermoFisher Scientific) ...
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bioRxiv - Microbiology 2024Quote: ... DNA extraction was performed utilizing commercial kits and following manufacturer’s protocol (Qiagen’s DNeasy 96 Blood & Tissue Kit, Qiagen). The amount and quality of extracted DNA was evaluated using PicoGreen fluorometer and NanoDrop spectrophotometer (ThermoFisher Scientific) ...
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bioRxiv - Cancer Biology 2024Quote: ... DNA was isolated using either QIAGEN Plasmid Plus Midi Kit or 28 QiaPrep Spin Miniprep Kit (Qiagen, 27104).
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bioRxiv - Cell Biology 2023Quote: ... Plasmid DNA was extracted using mini-prep plasmid extraction kit and/or Maxi-prep plasmid extraction kit (QIAGEN). The constructs were further confirmed by the Sanger sequencing.
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bioRxiv - Microbiology 2019Quote: ... Quantification of influenza virus nucleic acid load in tissues was performed in 96-well plates with 20µL final volume according to the manufacturer instructions (QuantiFast SYBR Green PCR, Qiagen, Toronto, Canada), 2µl of cDNA and a final concentration of 1µM of each primer ...
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bioRxiv - Systems Biology 2019Quote: ... A second diploid selection was conducted by inoculating 50 μL from the first diploid selection culture into a 96-well deep well plate (QIAGEN, #19579) containing 1.2 mL of liquid SC-LW medium per well ...
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bioRxiv - Evolutionary Biology 2021Quote: ... 2.5×106 cells per well were seeded in six-well plates and transfected using Effectene transfection reagent (Qiagen, Cat. No. 301425). The transfection mixtures contained the following plasmids ...
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bioRxiv - Evolutionary Biology 2021Quote: ... 2.5×106 cells were seeded per well in 6-well plates and transfected using Effectene transfection reagent (Qiagen, Cat. No. 301425). The transfection mixtures in Figure 5A contained 1 μg ...
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bioRxiv - Cell Biology 2019Quote: ... Transient transfections were carried out using just subconfluent cultures in 35 mm plates or in wells of a 6-well plate using DNA in the range of 0.3-2 µg/culture and the Polyfect reagent (Qiagen, Germantown, MD) and the manufacturer’s protocol ...
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bioRxiv - Genetics 2021Quote: ... S2 cells were seeded at 1 × 106 cells/ml in a 6-well plate and transfected using Effectene (Qiagen, Germantown, MD). For mobility shift assay (Figure 4) ...
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bioRxiv - Cell Biology 2020Quote: ... Transient transfections were carried out using just subconfluent cultures in 35 mm plates using DNA in the range of 0.3-2 µg/culture and the Polyfect reagent (Qiagen, Germantown, MD) and the manufacturer’s protocol (with 10 µl Polyfect reagent per 35 mm plate).
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bioRxiv - Immunology 2020Quote: Initial crystallization hits were obtained via sparse matrix screening in 96-well plates using commercially available crystallographic solutions (Qiagen, Toronto, ON). Optimization of crystallization conditions was performed in 24-well plate format using the hanging drop vapor diffusion method ...
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bioRxiv - Biochemistry 2022Quote: ... Transient transfections were carried out using just subconfluent cultures in 35 mm plates using DNA in the range of 0.3-2 µg/culture and the Polyfect reagent (Qiagen, Germantown, MD) and the manufacturer’s protocol (with 10 µl Polyfect reagent per 35 mm plate).
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bioRxiv - Cancer Biology 2022Quote: ... Exosomal miRNAs were profiled using Human Serum/Plasma miRCURY LNA miRNA PCR array (Qiagen, #YAHS-106Y, Plate Format: 2 × 96-well).
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bioRxiv - Molecular Biology 2022Quote: Nunc MaxiSorp™ 96-well plates were coated with 75 ng/well of penta-His antibody in PBS solution (Qiagen, 34660) and incubated overnight at 4 °C ...
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bioRxiv - Molecular Biology 2023Quote: ... Cells were seeded at 3 × 105 cells.well−1 in 6-well plates and transfected 24 h later with one pmiRGLO derivative construct per well (0.9 µg plasmid) using Effectene (Qiagen cat. #301425). After 3 days the cells were washed with PBS and lysed with Cell Culture Lysis Buffer (Promega cat ...
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bioRxiv - Cancer Biology 2023Quote: ... medium was removed and cells were lysed on the plate by addition of 3 ml of lysis reagent RLT Plus (Lot 169010027, Qiagen, Germany). 1 ml of lysate was transferred to a 2 ml Eppendorf tube ...
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bioRxiv - Plant Biology 2023Quote: ... Sand and rhizosphere samples were prepared by adding 250 mg of the collected substrate to a PowerBead plate and homogenized using a TissueLyser II (Qiagen, USA) at a frequency of 20 Hz for 10 minutes ...
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bioRxiv - Cancer Biology 2023Quote: ... MiRs (61 plus 3 controls) were quantified on a custom PCR plate using the miRCURY LNA miR SYBR Green PCR Handbook (Qiagen, Germany). PCR (40 cycles ...
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bioRxiv - Bioengineering 2024Quote: ... These expression cultures were set up in 500 uL volumes in deep-well 96-well plates (square wells used for maximum culture aeration; USA Scientific 1896-2800) sealed with porous tape (Qiagen 19571) and incubated at 37°C and 350 RPM for 20 hr ...
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bioRxiv - Microbiology 2020Quote: ... and purified using the RNAeasy Kit (Qiagen) according to manufacturer’s instructions ...
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bioRxiv - Cell Biology 2021Quote: ... or RNeasy Plus Mini Kit (Qiagen# 74134). mRNA was isolated and purified using Dynabeads™ mRNA Purification Kit (for mRNA purification from total RNA preps ...
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bioRxiv - Molecular Biology 2020Quote: ... the DNeasy Blood and Tissue Kit (Qiagen) or the AllPrep DNA/RNA Micro Kit (Qiagen ...