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Citations for Qiagen :
1801 - 1850 of 10000+ citations for Cyclic GMP Direct ELISA Kit 5 Plate since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2019Quote: ... The soluble fraction was loaded onto a column containing 5 ml Ni-NTA superflow resin (Qiagen), pre-equilibrated with buffer A ...
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bioRxiv - Immunology 2020Quote: ... The bacterial lysates were mixed for 1 h with 5 ml of Ni-NTA resin (Qiagen) that had been equilibrated with buffer A ...
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bioRxiv - Microbiology 2021Quote: ... sorokiniana were ground with two stainless steel beads (5 mm) using a TissueLyser (Qiagen, Hilden, Germany) at 30 Hz for 1 minute at room temperature and soaked in 1 ml of 100 mM sodium acetate buffer pH 5.0 at 70°C overnight ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... 50 mM Tris pH 8.0) using 5 mM stainless steel beads in a TissueLyser II (Qiagen). In vitro TPP1 assay was performed as described ...
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bioRxiv - Molecular Biology 2019Quote: ... 3 μL water and 5 μL 2x QuantiFast® SYBR® Green PCR Master Mix (Qiagen). Technical duplicates of this reaction mix were then analyzed on a Corbett Rotor-Gene 6000 real-time PCR cycler ...
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Establishment of Wolbachia strain wAlbB in Malaysian populations of Aedes aegypti for dengue controlbioRxiv - Microbiology 2019Quote: ... Mosquitoes were homogenised in 175 µL of 5% Chelex® solution using TissueLyser II machine (Qiagen) and with 5 µL of proteinase K (20 mg/mL ...
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bioRxiv - Biochemistry 2021Quote: ... The clarified lysate was applied to two tandemly-connected 5 ml Ni-NTA Superflow cartridges (Qiagen); the cartridges were washed with buffer containing 20 mM Tris pH 8.0 ...
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bioRxiv - Cancer Biology 2021Quote: ... sections were incubated in 0.08 M KOH for 5 min and washed by Buffer EB (Qiagen). Then ...
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bioRxiv - Microbiology 2020Quote: ... The proteolysis reaction products were then passed over a 5 mL Ni-NTA superflow cartridge (Qiagen) to remove TEV and uncleaved protein ...
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bioRxiv - Microbiology 2021Quote: ... and 5 h time points were immediately mixed with 2 x volume of RNAProtect® (Qiagen) using the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2022Quote: ... siRNAs used were RAPH1 siRNA #2 (Hs_RAPH1_2, SI00698642) and RAPH1 siRNA #5 (Hs_RAPH1_5, SI04300982) provided by Qiagen.
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bioRxiv - Cell Biology 2022Quote: ... A Locked Nucleic Acid (LNA) probe was used to detect centromere regions (5’-TTGGCTACACCATGAAAGCTT-3’; Qiagen). 20 μL of probe mix (250 nM LNA probe ...
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bioRxiv - Cancer Biology 2023Quote: Each qPCR reaction consisted of 5 μL of 2× QuantiNOVA SYBR Green PCR master mix (Qiagen), 0.5 μL of 10 μM forward and reverse primers each ...
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bioRxiv - Microbiology 2023Quote: ... Each reaction contained 5 μL DNA solution and PCR mixture with Taq Type-it (Qiagen®). PCR products were analyzed by capillarity electrophoresis on an ABl3130xl sequencer ...
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bioRxiv - Neuroscience 2023Quote: Isolated microglia were centrifuged for 5 min at 600xg and resuspended in RLT plus buffer (Qiagen) for extraction and purification of total RNA ...
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bioRxiv - Microbiology 2024Quote: ... One µg of purified RNA was treated twice with 5 µl of RNAse-free DNAse (Qiagen) and subjected to a final on column purification ...
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p110α-dependent hepatocyte signaling is critical for liver gene expression and its rewiring in MASLDbioRxiv - Cell Biology 2024Quote: ... The p110α deletion was confirmed by PCR and HotStar Taq DNA Polymerase (5 U/μl, Qiagen) using two primer pairs ...
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bioRxiv - Molecular Biology 2024Quote: ... 2.8□l of forward and reverse primer at 5□M concentration and 10□l QuantiNova (Qiagen), made up to a final volume of 20□l with nuclease-free water ...
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bioRxiv - Biophysics 2024Quote: ... The supernatant was transferred to a fresh tube and 5 ml of Ni-NTA resin (Qiagen) was added.
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bioRxiv - Neuroscience 2019Quote: ... Relative gene expression was measured using RT2 Profiler low-density PCR plates preloaded with qPCR primer assays for genes encoding GABA- and glutamate-related targets (PN: PARN-152ZA, Qiagen). Thermal cycling and data collection were accomplished using an ABI Real-Time PCR 7300.
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bioRxiv - Cell Biology 2019Quote: ... 1.2×106 cells were plated into each well of a 6-well plate and transfected with Sns and Eff-1 constructs (200ng each) using Effectene (Qiagen) per the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2020Quote: ... 5.6 x 105 cells were seeded in 4 ml DMEM in a 6-cm culture plate and transfected with targeting or control siRNA (Table 4) (from Qiagen) to a final concentration of 20 nM for each siRNA used ...
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bioRxiv - Systems Biology 2021Quote: ... Crystal screening was performed with a Gryphon robot (Art Robbins instruments, Sunnyvale, USA) using sitting-drop 96-well plates and Screening Suites (Qiagen). Optimization experiments were set up in 24-well hanging drop crystallization plates ...
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bioRxiv - Cancer Biology 2020Quote: ... Our panel of 88 target genes was prepared on a 96-well plate format with housekeeping genes and controls (RT2 PCR array, Qiagen). Expression was quantified on an ABI7500 real-time PCR machine (Applied Biosystems ...
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bioRxiv - Immunology 2020Quote: Single cells were sorted using a FACS Aria into 96-well plates containing 5μL of lysis buffer (TCL buffer, Qiagen 1031576) supplemented with 1% β-mercaptoethanol ...
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bioRxiv - Cancer Biology 2020Quote: Cells were seeded in 96-well plates (2 × 104 cells/well) and reverse-transfected with 100 ng/well of Cignal ISRE-Luc reporter (Qiagen) using Lipofectamine 3000 ...
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bioRxiv - Microbiology 2021Quote: ... 10 µl of the cell suspension from each dilution was dispensed into 24 wells of a 96 well plate containing 2 μl One-step RT-PCR enzyme (Qiagen), 10 μl 5x One-step RT-PCR buffer (Qiagen) ...
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bioRxiv - Evolutionary Biology 2022Quote: ... Individual females were homogenized in QIAGEN ATL Buffer in 96 well-plates with a sterile glass bead per well on a Tissue Lyser II (QIAGEN) at 23s/f for 2 minutes ...
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bioRxiv - Systems Biology 2019Quote: ... 2i/LN511 or PDMK/LN511 conditioned cells at 70% confluency in a well of a 6-well plate using RNeasy Plus Mini (Qiagen). Three biological replicates were prepared ...
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bioRxiv - Molecular Biology 2020Quote: ... catalysis was measured using a previously described assay (22) in which hexahistidine-tagged PARP-1 was immobilized on Ni(II)-NTA-coated plates (Qiagen) in the presence of NAD+/biotinylated-NAD+ (Trevigen) ...
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bioRxiv - Animal Behavior and Cognition 2020Quote: ... we transfected S2 cells in each well of a 6-well plate with 1 µg each of HA/GFP-tagged constructs using Effectene (Qiagen). After incubation for 3 days ...
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bioRxiv - Cancer Biology 2020Quote: ... we captured poly-adenylated mRNA from cell lysates using a 96-well plate with oligo(dT) grafted to the inner walls of each well (Qiagen). Next ...
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bioRxiv - Genetics 2019Quote: ... S2 and KC cell lines were stably transduced with the pMKInt-Hyg vector encoding RasV12Common and RasV12Rare cDNAs using 1000 ng of DNA in 6 well plates as instructed by Effectene transfection reagent (Qiagen). The following day ...
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bioRxiv - Genomics 2021Quote: ... were sorted into individual wells of a 96 well plate containing lysis buffer 2.5μL RLT Plus Lysis Buffer (QIAGEN, catalog # 1053393) with 1U/μL SUPERase-In (ThermoFisher ...
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bioRxiv - Immunology 2021Quote: ... single MR1-tetramer-binding cells from Peruvian participant 7-3 and blood bank donors 702A and 703A were sorted into 96-well plate coated with Vapor-Lock (Qiagen) containing Iscript cDNA synthesis mixture (Bio-Rad ...
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bioRxiv - Genetics 2019Quote: ... Converted samples were randomized in a 96-well plate and sequenced for each CpG on PyroMark Q96 ID provided by QIAGEN. The raw data were processed in PyroMark Q24 Software v2.5.8 by QIAGEN.
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Intrarenal B cells integrate in situ innate and adaptive immunity in human renal allograft rejectionbioRxiv - Immunology 2020Quote: ... and CD45+ Calcein+ DAPI-CD19+ CD38+ activated B cells were single-cell sorted into 96-well plates with catching buffer (RLT lysis buffer (Qiagen) with 1% 2-mercaptoethanol (Sigma-Aldrich)) ...
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bioRxiv - Microbiology 2020Quote: ... HEK293T cells in 6-well plates were transfected with 2 μg pIRES2-eGFP or Trim-HA-NUP153 derivatives using Effectene (Qiagen). At 24 h post-transfection ...
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bioRxiv - Immunology 2020Quote: ... 5 μl of amplified cDNA from each well of a 96-well plate were pooled and completed to 500 μl with PCR-grade H2O (Qiagen). Two rounds of 0.6X solid-phase reversible immobilization beads (AmpureXP ...
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bioRxiv - Immunology 2021Quote: ... and subsequent crystallization optimization was performed manually using hanging-drop vapor diffusion method with 15-well screw-cap crystallization plates (Qiagen). The H5.28-HA head complex was crystallized in 7-9% PEG 8000 ...
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bioRxiv - Genetics 2021Quote: ... frozen and lyophilised in a 2 ml 96 deep-well plate following which the samples were ground in the TissueLyser II (QIAGEN) using a steel ball in each well for 4-6 minutes at a frequency of 25 Hz ...
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bioRxiv - Immunology 2021Quote: Single cells were index-sorted using a FACS Aria into 96-well plates containing 5μL of lysis buffer (TCL buffer, QIAGEN 1031576) supplemented with 1% β-mercaptoethanol ...
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bioRxiv - Cell Biology 2022Quote: HMECs were transfected with 30 nM miRNA or 30 nM antigomiR (anti-miRNA) in 384-well plates using HiperFect (Qiagen), and the protocol described above for ‘High-content miRNA Screening’ was followed ...
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bioRxiv - Neuroscience 2022Quote: ... iPS-NES cells were plated in 6-well plates and lysates were collected in RNAprotect® Cell Reagent (QIAGEN, #76526) to stabilize the RNA ...
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bioRxiv - Plant Biology 2022Quote: Leaf tissue samples were collected and freeze-dried in a deep-well plate and ground in a TissueLyser II (QIAGEN) for 4-6 minutes at a frequency of 25 Hz ...
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bioRxiv - Microbiology 2023Quote: ... Cells were sorted into 96-well plates containing lysis buffer with proteinase K solution in PKD buffer (1:16) (Qiagen) and were stored at -80ºC ...
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bioRxiv - Neuroscience 2023Quote: ... Ampicillin-resistant colonies were selected from transformation plates and liquid bacterial cultures grown as a source of plasmid DNA (Qiagen QIAprep Spin Miniprep Kit ...
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bioRxiv - Neuroscience 2023Quote: ... and colonies yielding an ≈2.4 kB product were isolated from a duplicate patch plate and grown in liquid culture as a source of plasmid DNA (Qiagen QIAprep Spin Miniprep Kit ...
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bioRxiv - Molecular Biology 2023Quote: Primary monocytes were cultured in 24-well plates and then transfected with control non-targeting siRNA (Dharmacon) or siRNA targeting ERN1 (Dharmacon) using HiPerFect reagent (QIAGEN) following the instructions provided by the manufacturer ...
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bioRxiv - Microbiology 2023Quote: ... a single GFP+ or GFP-J-Lat 11.1 cell was sorted directly into each well of a 96-well PCR plate containing the pre-amplification mastermix consisting of 1 μL One-step RT-PCR enzyme (QIAGEN), 5 μL 5× One-step RT-PCR buffer (QIAGEN) ...