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Citations for Qiagen :
1901 - 1950 of 2272 citations for 4 15 Oppositene 1 7 Diol since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Bioengineering 2022Quote: ... Illumina sequencing-compatible Unique Dual Index (UDI) adapters were ligated onto pools using the QIAsep 1-step amplicon library kit (Qiagen). Library QC was performed using Qubit and Tapestation before sequencing on an Illumina MiSeq platform at the Duchossois Family Institute Microbiome Metagenomics Facility at the University of Chicago ...
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bioRxiv - Microbiology 2022Quote: ... DNA on the exterior of filtered capsids was digested for 1 hour at 25 °C with 20.3 units DNase (Qiagen: 79254) supplemented with 10 mM MgCl2 ...
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bioRxiv - Molecular Biology 2022Quote: ... The PCR products were purified by ethanol precipitation and 1% agarose gel purified using the QIAquick Gel Extraction Kit (Qiagen). Single-round E ...
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bioRxiv - Genetics 2022Quote: Total RNA was isolated from 50 to 100 μl of packed worms from wild type and brd-1(null) using the RNeasy Mini Kit (74104; Qiagen) and QIAshredder (79654 ...
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bioRxiv - Genetics 2022Quote: Total RNA was extracted from nearly 100 µl of tightly packed age-synchronized adult worm pellet collected in 1 ml TRIzol reagent provided by Qiagen RNeasy Mini kit (Cat ...
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bioRxiv - Immunology 2022Quote: ... For this analysis bacterial DNA was isolated either from feces or from 1 cm of mouse terminal ileum using QIAmp Fast DNA Stool kit (QIAGEN). qPCR was performed using SYBR Green I qPCR Master mix on LC480II Light cycler (Both Roche) ...
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bioRxiv - Microbiology 2022Quote: ... pH 8.0) containing 1 mg/mL of lysozyme and supplemented with 10 μL of Proteinase K (QIAGEN, cat. no. 19131). The mixture was incubated on a shaker at room temperature for 30 min with intermittent vortexing ...
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bioRxiv - Neuroscience 2022Quote: ... At day 28 ASOs were added (final concentration of 0.2, 1 or 7.5 μM) and RNA was harvested (RNeasy mini kit [Qiagen, Cat#: 74106]) after 5 days of treatment ...
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bioRxiv - Genomics 2022Quote: ... RNA (3 replicates each of V or E2 treated samples from donor 1 and donor 2) was DNAse treated and cleaned up using the RNeasy Mini kit (Qiagen) or the RNA Clean and Concentrator 5 kit (Zymo ...
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bioRxiv - Microbiology 2022Quote: ... and 150 μL DMEM supplemented with 2% FBS and homogenized using a TissueLyser II (2 cycles at 30 Hz, 1 min, Qiagen). Homogenates were clarified by centrifugation and frozen until plaque assay titration ...
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bioRxiv - Microbiology 2022Quote: ... including either whole fecal pellets or 200-300 uL of contents diluted 1:10 (w/v) in sterile PBS using the MagAttract PowerMicrobiome Kit (Qiagen), and prepped DNA libraries as previously described (36) ...
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bioRxiv - Microbiology 2022Quote: ... from 1 mL of culture were frozen at −80°C for later DNA extraction using the DNeasy PowerSoil Kit (Qiagen) according to the manufacturer’s procedure ...
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bioRxiv - Microbiology 2022Quote: ... an aliquot of the sample was scraped off the frozen stock and homogenized for 1 min at 25 Hz in PBS using a tissue lyzer (QIAGEN). Serial dilutions were prepared in PBS and plated on MacConkey Agar (OXOID ...
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bioRxiv - Neuroscience 2023Quote: ... human THP-1 macrophages (MACs) and acutely isolated mouse microglia was extracted using the RNeasy Plus Mini kit (Qiagen, 74136) following manufacturer’s instructions ...
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bioRxiv - Plant Biology 2023Quote: ... One zirconia ball and 500 μl of 100% methanol premixed with ribitol (20:1) were added and samples were subsequently homogenized in the Tissue Lyser (Qiagen) 3 min at 25 Hz ...
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bioRxiv - Plant Biology 2023Quote: Total cellular RNA was extracted from 8-day-old wildtype or mutant seedlings grown on 1/2MS medium with RNeasy Mini Kit and QIAshredder (Qiagen). Three independently prepared samples with RNA integrity number greater than 8.0 from each line were sequenced and analyzed at The Centre for Applied Genomics at SickKids Hospital (Toronto ...
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bioRxiv - Immunology 2022Quote: ... and homogenized twice for 1-5 minutes each at 25Hz using a TissueLyser LT sample disruptor (Qiagen, Part No. 85600) with a 12 tube Tissue Lyser LT adapter (Qiagen ...
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bioRxiv - Neuroscience 2022Quote: SARS-CoV-2 RNA was harvested from the cell medium at various time points post infection and stored in viral lysis buffer with RNA supplements (50 μl of sample medium in 560 μl of AVL buffer supplemented with 1% carrier RNA) (Qiagen). RNA was extracted using QIAamp Viral RNA Mini Kit (Qiagen) ...
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bioRxiv - Neuroscience 2022Quote: ... The lysate was centrifuged at 10000g for 10 minutes and the supernatant was allowed to bind with 1 ml of Ni-NTA beads (Qiagen) for 1 hour ...
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bioRxiv - Microbiology 2022Quote: ... A 1 μg of total RNA of each sample was reverse-transcribed to cDNA using RT2 first strand kit (Qiagen) according to manufactures protocol ...
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bioRxiv - Microbiology 2022Quote: ... Tissue sections collected for virology were disrupted in 1 mL of sterile PBS using the TissueLyser LT (Qiagen, Germantown, MD) at 30 Hz for 5 min twice ...
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bioRxiv - Immunology 2022Quote: ... The cDNA libraries (1 ng) were tagmented with the prepared single-loaded Tn5 transposase and afterwards cleaned using MinElute PCR kit (Qiagen) following the manufacturer’s instructions ...
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bioRxiv - Immunology 2022Quote: Total RNA was prepared from 1-2*106 sorted naïve (CD25− CD44lowCD62high) or effector (CD25− CD44highCD62low) CD4+ T-cells using RNeasy Mini Kit (Qiagen Inc.) as described in manufacturer’s protocol including an on-column digestion step (RNaseFree DNase Set ...
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bioRxiv - Systems Biology 2024Quote: ... To this 200uL of resuspended pellet we added 1 volume (200uL) of the QX1 buffer from the Qiaex2 DNA extraction kit (Qiagen) [56] ...
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bioRxiv - Synthetic Biology 2024Quote: ... Illumina sequencing-compatible Unique Dual Index adapters were ligated onto the pools using the QIAseq 1-step amplicon library kit (Qiagen). Library quality control was performed using Qubit and TapeStation and sequenced on Illumina MiSeq platform to generate 2 × 250 bp reads.
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bioRxiv - Systems Biology 2024Quote: ... and left brain hemisphere using the phenol-chloroform extraction method with 1 mL of Qiazol Lysis Reagent (Qiagen, Hilden, Germany), as previously described in Ederer et al ...
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bioRxiv - Molecular Biology 2023Quote: ... Worms were snap frozen in liquid nitrogen before being ground into a fine powder for 1 min by a stainless-steel bead in a TissueLyser LT (Qiagen) operating at 50 oscillations per second ...
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bioRxiv - Microbiology 2024Quote: ... while samples for RNA-based analyses were mixed at a 1:10 ratio of LifeGuard Soil Preservation (Qiagen, Hilden Germany) before being put on dry ice ...
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bioRxiv - Cancer Biology 2024Quote: ... A total of 1 μg of RNA was used as input for cDNA synthesis reactions by using the Omniscript RT Kit (Qiagen) along with random hexamers ...
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bioRxiv - Molecular Biology 2024Quote: ... The fidelity of the PCR reaction was checked using a 1 % agarose gel and then the reaction cleaned up using a PCR clean up spin column kit (Qiagen). Finally the concentration of the purified PCR product was checked on a NanoDrop spectrophotometer (Thermo-Fisher).
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bioRxiv - Microbiology 2024Quote: ... one fecal pellet per procedure was placed in a 2 mL round-bottom tube containing a 0.5 cm diameter stainless steel bead and 500 µL of sterile phosphate-buffered saline (PBS) and agitated at 1/30s using a TissueLyser II (Qiagen) for 60 seconds ...
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bioRxiv - Microbiology 2024Quote: ... HsMX1 or MmMx1 and infected or not with IAV at MOI 1 were extracted using the miRNeasy Mini kit (Qiagen). RNA quality was analysed using a BioAnalyzer (Agilent ...
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bioRxiv - Microbiology 2024Quote: 0,5-2 × 106 cells were collected 8 h or 24 h following mock infection or infection with A/Victoria/3/75 infection at MOI 1 and total RNAs were isolated using the RNeasy kit with on-column DNase treatment (Qiagen). cDNAs were generated using 125 ng RNA (High-Capacity cDNA Reverse Transcription Kit using 250 nM oligodT instead of the provided random primers ...
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bioRxiv - Microbiology 2024Quote: ... corresponding to the three extraction protocols we used: (NM-1) one negative control for the kit DNeasy Blood and Tissue (Qiagen) used for mosquito DNA extraction ...
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bioRxiv - Neuroscience 2024Quote: ... DRG and paw skin RNA was extracted using a RLT buffer:2-mercapto ethanol mixture in a 100:1 ratio/RNeasy (Qiagen) RNA mini kit according to the manufacturer’s instructions and spinal cord and spleen RNA was extracted using TRizol (Invitrogen)/RNeasy RNA mini kit ...
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bioRxiv - Microbiology 2023Quote: ... At the last passage parasite pellets with ∼1 × 108 parasites were collected for DNA isolation using DNeasy blood and tissue kit (Qiagen).
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bioRxiv - Cell Biology 2024Quote: ... Cells were directly lysed in the well using 350 µL of lysing solution (1% 2-mercaptoethanol in Buffer RLT; RNeasy Mini Kit, 74106, Qiagen) by pipetting over the well ...
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bioRxiv - Developmental Biology 2024Quote: ... thresholds were used to prepare Reduced Representation Bisulfite Sequencing (RRBS) libraries using the Ovation RRBS Methyl-Seq System 1-16 (NuGen) and the EpiTect Fast DNA Bisulfite kit (Qiagen). Libraries were sequenced using the NextSeq500 platform (Illumina ...
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bioRxiv - Evolutionary Biology 2024Quote: ... The bits containing the powder was immediately transferred and rinsed in a 2 ml Eppendorf (DNA/RNA and DNase/RNase free tube) containing 1 ml of Tissue Lysis buffer ATL from the QIAamp DNA Micro Kit (Qiagen) and stored at 4°C until the DNA extraction process (i.e ...
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bioRxiv - Genetics 2024Quote: Total genomic DNA was extracted from 10% suspension of fin clips (<1 mm2) using the DNeasy Blood and Tissue Kit (Qiagen) according to the manufacturer’s protocols ...
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bioRxiv - Immunology 2024Quote: ... cDNA was diluted to 5 ng/µl in RNase-free water and 1 µl used for real-time qPCR with Qiagen QuantiNova SYBR Green Master Mix (Qiagen) in a StepOnePlus Real-Time PCR System (Applied Biosystems ...
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bioRxiv - Genomics 2024Quote: ... The resulting lysate was then made up to 3 mL with QIAzol Lysis Reagent and mixed thoroughly before 1 mL lysate aliquots were processed using RNeasy Lipid Tissue Kit 74804 (QIAGEN) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2024Quote: ... mixed with 1 mL phenol:chloroform:isoamyl alcohol (25:24:1 at pH 8) at 70°C for 12 min and bead beating for 2 min (Tissue Lyser II, Qiagen). The mixture was centrifuged at 4°C for 3 min at maximum speed and the aqueous phase was transferred to a new reaction tube ...
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bioRxiv - Cell Biology 2024Quote: ... The genomic DNA fragment was separated by electrophoresis using a 1% agarose gel and purified using QIAquick Gel Extraction Kit (QIAGEN). The genome sequence of drp-1 was determined by Sanger sequencing using primers listed in Supplementary Table S2.
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bioRxiv - Cancer Biology 2024Quote: ... Isolated RNA was retro-transcribed to cDNA using 1 μg of RNA per reaction with either the QuantiTect Reverse Transcription Kit (205311, QIAgen) or the High-Capacity cDNA Reverse Transcription Kit (4368813 ...
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bioRxiv - Microbiology 2023Quote: ... Cells were sorted into 96-well plates containing lysis buffer with proteinase K solution in PKD buffer (1:16) (Qiagen) and were stored at -80ºC ...
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bioRxiv - Microbiology 2023Quote: ... RNA was eluted from the column in RNase/DNase free H2O and any residual DNA contamination was removed with a 1 hour off-column DNase treatment (Qiagen). The RNA was purified following the RNeasy Mini-Prep Kit following manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2023Quote: ... Proteinase K 20mg/ml was added and incubated at 65 for 1 hour and DNA was purified with PCR purification Kit from Qiagen. Then ...
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bioRxiv - Bioengineering 2023Quote: ... PCR products were resolved on 0.5x Tris-acetate-EDTA (TAE) 1 % agarose gels and the corresponding bands were cut out and purified using the QIAquick Gel Extraction kit (Qiagen). Restriction enzymes and T4 DNA ligase were obtained from NEB and Thermo Fisher Scientific ...
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bioRxiv - Cancer Biology 2023Quote: ... TetON-plko constructs were digested using AgeI and EcoRI restriction enzyme and the 8.8 kb fragment was purified from a 1% agarose gel using the Qiagen Gel Extraction kit (Qiagen, 28704). Annealed shRNA oligos and digested TetON-pLKO constructs were mixed together with T4 DNA Ligase (NEB ...