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Citations for Qiagen :
1701 - 1750 of 2272 citations for 4 15 Oppositene 1 7 Diol since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genetics 2020Quote: ... The PCR product was separated on a 1 % agarose gel and purified using the QIAquick Gel Extraction Kit (Qiagen). 60 ng of each probe were labeled with dATP [α-32P] (DECAprime kit II ...
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bioRxiv - Immunology 2020Quote: Lung tissue from individual mice were homogenized in 1 mL of TRIsure reagent with TissueLyser II (Qiagen No. 853000) and as per the manufacturer’s instructions and samples were centrifuged for 15 min at 12,000 x g at 4°C to remove cellular debris ...
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bioRxiv - Biochemistry 2020Quote: ... The amplified genes were then electrophoresed in 1% agarose gel followed by purification using PCR purification kit (Qiagen, USA). Both purified genes and plasmids were digested with Nde I and Not I prior to ligation using the T4 DNA ligase (New England Biolabs ...
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bioRxiv - Genomics 2021Quote: ... and an aliquot of 300-500uL was immediately mixed at 1:2.76 volume ratio with fluid from a Paxgene blood RNA tube (Qiagen), whilst the remainder was stored on ice for flow cytometry analysis ...
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bioRxiv - Biochemistry 2020Quote: ... proteins with log fold change >1 and Z-score > 2 were further analyzed in Ingenuity Pathway Analysis (IPA, Qiagen) to determine pathways enriched by the bait proteins ...
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bioRxiv - Biochemistry 2021Quote: The LRH-1-10CA-TIF2 complex was concentrated to 5 mg/mL and screened using the Classics screen (Qiagen) and a Phoenix Liquid Handler (Art Robbins Instruments ...
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bioRxiv - Genomics 2020Quote: ... first strand synthesis was performed by incubating the pucks in 200 μL of reverse transcription solution (Maxima 1x RT Buffer, 1 mM dNTPs, 2 U/μL Lucigen NxGen RNAse inhibitor, 2.5 μM template switch oligo with Qiagen #339414YCO0076714 ...
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bioRxiv - Developmental Biology 2020Quote: ... RNA was extracted from DF-1 cells and HH27 whole chick heads using the RNeasy Plus Kit (Qiagen, 74136) following the manufacturer’s directions ...
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bioRxiv - Cell Biology 2020Quote: ... and A10 encoding constructs or 1 μg Shh constructs with or without 0.5 μg Scube2 by using Polyfect (Qiagen). Cells were grown for 2 days or 3 days for Disp−/− rescue experiments at 37°C with 5% CO2 in DMEM containing 10% fetal calf serum and penicillin-streptomycin (100 μg/mL) ...
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bioRxiv - Microbiology 2021Quote: ... PCR products were analyzed on a 1% agarose gel and bands gel purified using QIAquick gel extraction kit (Qiagen). Samples were sequenced via Sanger sequencing (Genewiz ...
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bioRxiv - Molecular Biology 2020Quote: ... 15uL of the sample was de-crosslinked overnight at 65°C with 85ul of decrosslinking buffer (0.1M NaHCO3, 1% SDS) before clean up using QiaQuick PCR clean up kit from Qiagen.
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bioRxiv - Molecular Biology 2020Quote: ... cells were washed with PBS and the pellet was resuspended in 1 ml PBS containing RNAse A (Qiagen, 19101) at a final concentration of 0.2 mg/ml ...
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bioRxiv - Immunology 2020Quote: ... were homogenised in a Precellys 24 homogeniser with CK28 Hard tissue homogenizing 2.0 ml tubes (Bertin) and 1 ml of RLT buffer (Qiagen) supplemented with 1%(v/v ...
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bioRxiv - Genomics 2021Quote: ... DNA gets separated from proteins and cellular debris using proteinase K (20mg mL-1; Qiagen, Inc., Valencia, CA, USA), precipitated with ethanol and cleaned up ...
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bioRxiv - Genomics 2021Quote: ... Illumina reads for each genome were mapped to the yeast reference genome (R64-2-1, yeastgenome.org) using CLC-Genomics software (Qiagen). Resulting read mapping files were then subjected to copy number and heterozygous single nucleotide polymorphism (hetSNP ...
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bioRxiv - Plant Biology 2023Quote: ... Leaf discs were ground in 1 mL sterile water with the help of a tissue lyser (QIAGEN, TissueLyser II) using 1.5 mL safe-lock Eppendorf tubs containing three 2.4 mm metal beads ...
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bioRxiv - Molecular Biology 2022Quote: RNA was isolated from 1 × 106 BMDM from WT or p38γ/δKIKO male mice using the RNeasy kit (QIAGEN). Biological replicate libraries were prepared using the TruSeq RNA library prep kit (Illumina ...
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bioRxiv - Cell Biology 2023Quote: ... Cells were harvested by centrifugation (2500 x g, 3.5 min) and washed with 1 mL of RNAse-free water (Qiagen) before being spun down (10k rpm ...
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bioRxiv - Immunology 2023Quote: ... 14-666-315) containing 1 mL sterile PBS and one 5 mm stainless steel bead (QIAGEN Cat. No. 69989). Nucleic acids were isolated from excised granulomas by homogenizing in TRIzol (Invitrogen ...
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bioRxiv - Molecular Biology 2023Quote: ... Clarified bacterial lysates from a 1 l culture were bound to a 0.5 ml column of Ni-NTA resin (Qiagen) by gravity flow ...
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bioRxiv - Genetics 2023Quote: ... leaf tissues were collected and homogenized in 400 μL of TPS buffer (100 mM Tris-HCl, 10 mM EDTA, 1 M KCl, pH8.0) using TissueLyser II (Qiagen), followed by incubation for 20 min at 75°C ...
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bioRxiv - Immunology 2023Quote: ... Primers were designed using the NCBI primer-designing tool (see Table 1) or ordered as validated primers from Qiagen. The mRNA expression levels were plotted as relative gene expression to β-actin (2-ΔCt) ...
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bioRxiv - Microbiology 2022Quote: ... DNA was analyzed on an agarose gel (1%) and purified by using the QIAquick gel extraction kit (Qiagen #28706). DNA was then digested and primer dimers were ligated at 16°C overnight ...
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bioRxiv - Plant Biology 2023Quote: ... Total RNA (1 μg) was treated with DNase and converted into cDNA using the QuantiTect Reverse Transcription kit (Qiagen), according to the manufacturer’s protocols ...
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bioRxiv - Cell Biology 2023Quote: ... Cells were treated with Angiotensin-II (1 μM) alone or in combination with DAPT (10 μM) or siNotch1 (Qiagen) (50 nM ...
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bioRxiv - Bioengineering 2023Quote: ... total RNA was extracted from about 1 × 106 cells from all groups using RNeasy Mini Kit (Qiagen, Valencia, CA), then using the random hexamer primer and M-MuLV reverse transcriptase kit (Fermentas ...
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bioRxiv - Developmental Biology 2023Quote: Blastocysts were washed with DPBS containing 1 mg/ml polyvinylpyrrolidone (PBS-PVP) and transferred into 50 μl droplets of 0.1% protease (Qiagen) to remove the zona pellucida ...
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bioRxiv - Cell Biology 2023Quote: ... genomic DNA was extracted by incubation with 6 ml of lysis buffer (50 mM Tris pH 8.0, 50 mM EDTA, 1% SDS, 0.1 mg/ml proteinase K (Qiagen, 19131)) at 55°C for 16 hours ...
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bioRxiv - Cancer Biology 2023Quote: ... V2 and V3 amplicons were resolved in the 1% agarose gel and purified using the gel purification kit (Qiagen). Each cDNA was cloned into pDONR201 (Invitrogen ...
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bioRxiv - Molecular Biology 2023Quote: ... genomic DNA (gDNA) was extracted from J-Lat cells 10.6 and HIV-1 negative CD4+ T cells with the QIAcube (Qiagen), using the QiaAmp DNA mini kit (Qiagen) ...
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bioRxiv - Molecular Biology 2023Quote: ... the Rainbow proviral HIV-1 DNA dPCR assay was performed on a QIAcuity Four digital PCR platform (Qiagen, Germany) with at least 500 ng (CD4+ T cell ...
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bioRxiv - Immunology 2023Quote: ... and approximately 1 µg of isolated RNA was converted to cDNA using RT2 First Strand Kit (Qiagen, Hilden, Germany). cDNA was diluted (1:10 ...
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bioRxiv - Systems Biology 2023Quote: ... Large fragments (200-400 bp) were extracted from a 1% agarose gel with QIAquick Gel Extraction Kit (Qiagen, Netherlands). The final library was sequenced as 85 bp long single-end reads on a NextSeq™ 550 (Illumina ...
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bioRxiv - Physiology 2023Quote: ... including DNase 1 treatment (Monarch Total RNA Miniprep kit, #T2010, New England Biolabs, USA and QIAGEN RNeasyMini kit, #74104). Purity and quantity of RNA were assessed by determining the optical density (OD ...
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bioRxiv - Cancer Biology 2022Quote: ... and 1 µg of each sample was used to prepare 20 µL cDNA using RNeasy Plus Mini Kit (Qiagen). Human RT2 Profiler™ PCR Array ...
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bioRxiv - Microbiology 2022Quote: ... 1 to 2 mL of culture were mixed with the same volume RNAprotect™ Bacteria Reagent (Qiagen, Hilden, Germany) incubated for 10 min and centrifuged ...
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bioRxiv - Physiology 2022Quote: ... Lysates were centrifuged at 100,000 x g for 1 h and the supernatant were affinity purified on Ni-NTA resin (Qiagen) by batch binding for 30 min-1h ...
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bioRxiv - Microbiology 2022Quote: ... and then homogenized tissues in 1 ml PBS containing a penicillin/streptomycin mixture by using a TissueLyser II (Qiagen) at 30-Hz oscillation frequency for 3 min ...
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bioRxiv - Microbiology 2022Quote: Airway epithelium cultured on 6.5-mm Transwell membranes was washed and treated for 1 min at RT with RLT buffer (Qiagen) with 0.01 % β-mercaptoethanol (Sigma-Aldrich) ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... The lysate was centrifuged at 50,000×g for 10 min and to the supernatant 1 mL of 50% Ni−NTA (Qiagen) was added and incubated for 1 h at 4 °C ...
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bioRxiv - Genomics 2024Quote: ... Homogenization buffer for RNA purification was made by adding 1:100 beta-mercaptoethanol to Buffer RLT (Qiagen, Valencia, CA) and kept on ice until use ...
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bioRxiv - Immunology 2024Quote: ... The cells were then washed with 1× PBS and treated with 100 μg/mL of RNase A (Qiagen, USA) followed by staining with 50 μg/mL of propidium iodide (Sigma-Aldrich ...
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bioRxiv - Microbiology 2024Quote: ... Material was ruptured two times for 1 min at 30 Hz in a TissueLyser (Qiagen Inc., Germantown, MD, USA). Between and after these two steps of tissue disruption ...
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bioRxiv - Systems Biology 2023Quote: ... A culture volume equal to 3 mL of OD = 1 was added to 6 mL RNAprotect Bacteria Reagent (Qiagen), vortexed ...
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bioRxiv - Genomics 2023Quote: ... After overnight proteinase K digestion in Lysis Buffer (BNG) and 1-hour treatment with RNAse A (Qiagen, MD,USA), plugs were washed 4 times in 1×Wash Buffer (BNG ...
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bioRxiv - Cancer Biology 2024Quote: ... doxycycline was added at (0.1 μg/ml, 1 μg/ml (CHRDL2 +) or 10 μg/ml (CHRDL2 ++) RNA was extracted (RNeasy, QIAGEN) and quantified by real-time reverse transcriptase polymerase chain reaction (qPCR ...
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bioRxiv - Genomics 2023Quote: ... 1×106 cells were harvested from each culture and DNA was extracted using QIAamp DNA mini Kit (QIAGEN 51304). STR analysis was performed with PowerPlex 21 System (Promega DC8902 ...
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bioRxiv - Cancer Biology 2023Quote: ... and 9-10 mL of Buffer BB (1/3 of the lysate volume, From QIAGEN Plasmid Plus Midi Kit) was added ...
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bioRxiv - Immunology 2023Quote: The post-caval lobe of the lung was weighed and homogenized in 1 mL of PBS (pH 7.4) using a TissueLyser LT (Qiagen). RNA was extracted using TRIzol™ LS Reagent (Invitrogen ...
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bioRxiv - Bioengineering 2023Quote: Each body part (legs and thorax) was homogenized individually for 3 × 1 minute at 30 Hertz using TissueLyser (Qiagen) and glass beads (#11079110 ...