Labshake search
Citations for Qiagen :
1901 - 1950 of 3161 citations for 1 1' 3' 1'' Terphenyl 4 4'' dimethanamine 5' 4 aminomethyl phenyl 9CI since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2021Quote: ... 30mg of whole spleen RNA was extract via 30 second homogenization in RLT lysis buffer containing 1% β-mercaptoethanol per RNeasy kit (Qiagen). RNA was reverse transcribed into cDNA using iScript cDNA synthesis kit (BioRad) ...
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bioRxiv - Molecular Biology 2020Quote: Genomic DNA was extracted from ∼1 x 107 cells using a modified version of the Gentra Puregene Cell Kit cell extraction protocol (Qiagen 158745). Integrity of genomic DNA and absence of contaminating RNA was confirmed via 1% agarose 1x TAE gel electrophoresis ...
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bioRxiv - Biochemistry 2020Quote: ... was mixed with selection plasmid pCoBlast (1:10) and the mixture was used for cells transfection by Effectene Transfection Reagent (QIAGEN, Germany) according to the manufacturer’s protocol ...
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ST6GAL1-mediated heterogeneity in sialic acid levels potentiates invasion of breast cancer epitheliabioRxiv - Cancer Biology 2020Quote: ... Real time PCR was performed with 1:2 diluted cDNA using SYBR green detection system (Thermo Fischer Scientific, F415L) and Rotorgene Q (Qiagen, 9001560). 18 S rRNA gene was used as internal control for normalization ...
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bioRxiv - Cell Biology 2021Quote: ... podocytes were washed three times with warm 1× PBS and the viral DNA was extracted using DNeasy Blood & Tissue kit (Qiagen #69504) [26] ...
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bioRxiv - Plant Biology 2020Quote: ... and thiols and disulphides were extracted in 1 mL of ice-cold 0.1 M HCl using a Tissue-Lyser (Qiagen, Hilden, Germany) and two 3-mm glass beads (30 Hz ...
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bioRxiv - Microbiology 2021Quote: ... pCDNA-SARC-CoV-2 Wuhan-Hu-1 P681H S plasmid was then extracted using the QIAprep Spin Miniprep Kit (Qiagen N.V.) and Sanger sequencing was used to confirm incorporation of the mutation.
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bioRxiv - Microbiology 2020Quote: ... frozen in liquid nitrogen for 1 minute and homogenised by shaking at 40 Hz in a Tissue Lyser LT (Qiagen, Germany). The volume of elution buffer AE was adjusted to 200 μL for engorged females ...
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bioRxiv - Cell Biology 2021Quote: ... Plasmid sgRNA CRISPR porthos was co-transfected (1 µg of total DNA per well) with Effectene Tranfection Reagent (Qiagen, Hilden, Germany) following the manufacturer’s protocol ...
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bioRxiv - Evolutionary Biology 2022Quote: ... DNA was extracted once with 350 μL Phenol:Chloroform:Isoamyl Alcohol (25:24:1) in a phase-lock tube (Qiagen cat. no. 129046) and centrifuged 5 min at 16,000xg followed by extraction with 350 μL chloroform ...
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bioRxiv - Neuroscience 2022Quote: DIV14 neurons treated with 1 µM glutamate for the times indicated in the text then harvested using the Qiagen RNeasy Mini kit (Qiagen, 74104) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2022Quote: ... The qRT-PCR was performed using 20 miRNA primers (Supplementary Table 1) and miScript SYBR Green PCR Kit (Qiagen, Hilden, Germany). RNA U6 was used as the endogenous control ...
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bioRxiv - Cell Biology 2022Quote: ... diluted 2-times and 1 μl was taken for each PCR reaction (20 ul) containing: 0.02 U HotStarTaq® DNA polymerase (Qiagen, Germany), 1 x PCR buffer ...
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bioRxiv - Cancer Biology 2022Quote: ... These specific colonies were amplified by culturing in liquid LB-medium with ampicillin (1:1000) followed by plasmid isolation using QIAprep Spin Miniprep Kit (Qiagen #27104), according to the manufacturer ...
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bioRxiv - Microbiology 2021Quote: ... These cells were incubated overnight in MEM-α media free of FBS and supplemented with 1% P/S followed by collection of Supernatant (conditioned media) and total RNA extraction (Cat# 74134, Qiagen, Germany). The resulting samples were stored at −80°C until analyzed further in downstream applications.
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bioRxiv - Molecular Biology 2022Quote: ... In all samples disruption occurred in 1 min intervals to avoid heat generation derived from the TissueRuptor II (catalogue #9002755, Qiagen, MD)29.
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bioRxiv - Developmental Biology 2022Quote: ... Each of the three reaction pellets were allowed to resuspend in TE for 1 hour at room temperature then pooled into one tube and purified across three Qiaquick PCR purification columns (Qiagen, 28104), eluted in the provided Elution Buffer and combined ...
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bioRxiv - Genomics 2022Quote: ... Trachea was dissected and immersed in 1 mL PBS in a 2 mL microcentrifuge tube (Fisherbrand, 14-666-315) containing one stainless steel bead (QIAGEN, 69989). After the homogenization ...
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bioRxiv - Immunology 2022Quote: ... macrophages were lysed with 300μL lysis buffer with 1% v/v β-mercaptoethanol (Themo #12183025) and passaged through a QIAshredder homogenizer column (Qiagen #79656). Total RNA was isolated (Themo #12183025 ...
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bioRxiv - Microbiology 2022Quote: ... with 1 min rest on ice in between each round of bashing) on a TissueLyser II beadmill (Qiagen, Valencia, CA, USA), then clarified by centrifugation at 2000 rpm at 4°C for 5 min in a refrigerated tabletop centrifuge (Beckman Coulter ...
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bioRxiv - Cell Biology 2020Quote: ... 350μL RLT buffer supplemented with 1% pure β-mercaptoethanol were added and the cell slurry was transferred onto a QIAshredder spin column (Qiagen). Subsequently ...
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bioRxiv - Evolutionary Biology 2020Quote: Total RNA was extracted from cells (1-2 wells, 6 well plate) using an RNeasy Plus Mini Kit (Qiagen, Hilden, Germany), including a DNase step to remove residual DNA ...
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bioRxiv - Zoology 2020Quote: ... SDS and 100 µg/ml proteinase K) and incubated at 56°C for 1 h before extraction using a QIAamp DNA Mini Kit (Qiagen, Germany) according to the manufacturer’s directions ...
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bioRxiv - Immunology 2020Quote: ... estimation of base-editing efficiency in the bulk population was performed as follows: ~1×106 cells were collected from which genomic DNA was isolated using the DNeasy Blood & Tissue Kit (Qiagen 69506). This was followed by PCR amplification of the desired region using DreamTaq Polymerase (Thermo Fisher Scientific EP0705) ...
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bioRxiv - Genomics 2021Quote: ... PCR amplicons were run on a 1% agarose gel and then purified using the QIAquick Gel Extraction kit (Qiagen cat # 28706). 1μg of DNA was digested with HindIII (New England Biolabs cat # R0104S ...
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bioRxiv - Physiology 2020Quote: ... the samples were thawed and physically disrupted using a beadmill (1 min max speed) using a TissueLyser LT (Qiagen, Hilden, DE) and then exposed to an in-house phenol-chloroform extraction protocol including an extra chloroform step and several RNA washing steps ...
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bioRxiv - Immunology 2020Quote: ... B220+Fas+CD38- CD45.1+ or CD45.1-) were sorted from immunized recipients directly into RLT buffer supplemented with 1% β-ME (Qiagen, Hilden, Germany). mRNA was extracted with an RNeasy Micro Kit (Qiagen ...
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bioRxiv - Synthetic Biology 2020Quote: ... For SARS-CoV-2 antigen detection the blots were incubated with 1:2000 Anti-His mouse monoclonal primary antibody (Qiagen, #34660) and then incubated with 1:10000 peroxidase labelled anti-mouse IgG secondary antibody (GE Healthcare) ...
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bioRxiv - Genetics 2021Quote: ... and end-point PCRs were performed following 34 cycles (94 C 30 sec, 58 C 30 sec, 72 C 1 min) using the HotStarTaq Plus DNA polymerase (Qiagen, Canada) according to manufacturer’s protocol.
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bioRxiv - Genetics 2021Quote: ... S2 cells were seeded at 1 × 106 cells/ml in a 6-well plate and transfected using Effectene (Qiagen, Germantown, MD). For mobility shift assay (Figure 4) ...
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bioRxiv - Neuroscience 2021Quote: ... Amplicons were separated on a 1-2% agarose gel and appropriate bands were excised and isolated using a gel extraction kit (Qiagen, USA). These fragments were inserted into the pcDNA3.1-based plasmid or cFUGW lentivirus vector using the T4 ligase (New England Biolabs ...
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bioRxiv - Microbiology 2020Quote: ... or remaining mosquito bodies were collected separately in a 2ml Eppendorf Safe Lock tube with 250μl DMEM (2% FBS, 1% Sodium Pyruvate) with 25 mg/ml Amphotericin B and a stainless steel bead (Qiagen, Hilden, Germany). Samples were stored at −80°C.
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bioRxiv - Immunology 2021Quote: ... amplicons were analyzed in 1 % agarose gel and bands corresponding to approximately 600 bp were purified with QiAquick gel extraction kit (Qiagen, USA). All the reactions were carried out in triplicates and pooled together during the gel purification ...
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bioRxiv - Immunology 2021Quote: ... Freshly harvested or in -80°C stored organ in 1 ml complete cell culture medium was homogenized using a metal bead at 25 Hz for 1 - 1.5 min by TissueLyser (Qiagen, Hilden, Germany). After centrifugation at 3500g for 5 min ...
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bioRxiv - Developmental Biology 2020Quote: ... and quantitative RT-PCR was performed using SYBR-based primers (Supplemental Table 1) and RT SYBR Green qPCR Mastermix (Qiagen, #330509) on a CFX96 Touch Thermal Cycler (Bio-Rad ...
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bioRxiv - Genomics 2020Quote: ... total genomic DNA of FAFU-HL-1 was extracted using a Qiagen DNeasy Plant Mini kit following the manufacturer’s instructions (Qiagen, Valencia, CA). For ONT sequencing ...
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bioRxiv - Immunology 2021Quote: ... plasmid DNA was extracted for each sample from a 1 ml overnight culture using the QIAprep Spin Miniprep Kit (Qiagen, 27104). Next ...
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bioRxiv - Cancer Biology 2023Quote: ... washed in ice-cold PBS and resuspended in 350μl of Buffer RLT Plus supplemented with 1% (v/v) β-mercaptoethanol from the RNeasy Plus Mini Kit (Qiagen, 74134). RNA was extracted using the same kit according to manufacturer’s instructions and quality was assessed using the Agilent 2200 TapeStation System (Agilent ...
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bioRxiv - Biochemistry 2022Quote: ... Memorial Sloan Kettering Cancer Center) at a dilution of 1:5,000) and His (mouse anti-penta-His antibody (Qiagen, cat. #34660) at a dilution of 1:10,000 ...
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bioRxiv - Immunology 2023Quote: DNA was extracted from 1×10^6 CD4 Th cells/ replicate using the Qiagen DNA mini kit according to manufacturer’s instructions (Qiagen Hilden, Germany). DNA was extracted from 10ng of mouse brain tissue as previously described13 using NEB Monarch High molecular weight DNA extraction kit according to manufacturer’s instructions (NEB Cat #T3050).
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bioRxiv - Evolutionary Biology 2022Quote: ... The concentration of the resulting His-tagged ß-CATENIN-1 fragments was quantified via quantitative Western blotting with a Penta-His antibody (Qiagen) and a recombinant His-tagged protein as external standard.
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bioRxiv - Cell Biology 2023Quote: ... total RNA was isolated from C2C12 myoblasts transfected with GFP-empty vector or GFP-Δ(1-240)JPh1 using the RNeasy-plus mini kit (QIAGEN) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2023Quote: DNA was extracted from NCI-PC35-1 and NCI-PC35-2 organoids using an AllPrep DNA/RNA Mini Kit (Qiagen 80204) according to the manufacturer’s protocol for animal cells ...
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bioRxiv - Microbiology 2023Quote: ... a small amount of tissue (>25 mg) was added to 1 mL of TRIzol with a single 5mm stainless steel bead (Qiagen, Germany) and homogenised using a TissueLyser II (Qiagen ...
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bioRxiv - Neuroscience 2022Quote: ... 30µL of DNase and RDD buffer (1:7 ratio) containing solution from the RNase-Free DNase Set (Qiagen, Catalog No. 79254) was incubated on the columns at room temperature for 15 minutes before a second and third round of RNA was performed as well as a final spin to ensure the column was dry ...
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bioRxiv - Cell Biology 2022Quote: ... were digested with DpnI for 1 hour at 37°C and subsequently purified with the QIAquick Gel Extraction Kit (Qiagen #28704). They were ligated with homemade Gibson Master Mix (Table S1 ...
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bioRxiv - Neuroscience 2024Quote: Total RNA was isolated from the cortical brain and cochlea samples from 1-month-old mice using RNA extraction kit and Qiazol Reagent (RNeasy mini kit; Qiagen, Germany), and DNase treatment was performed (RNase-free ...
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bioRxiv - Microbiology 2024Quote: ... RNA samples were initially preserved with 1 mL of activated sludge (i.e., suspended phase) in 9 mL of LifeGuard Soil Preservation (Qiagen, Hilden Germany). This solution was thawed and centrifuged (16,000 g for 1-minute ...
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bioRxiv - Microbiology 2024Quote: ... samples were thawed and homogenized at a frequency of 26/s for 1 minute in a TissueLyser II (Qiagen, Germantown, MD) and centrifuged at 16,500xg for 5 minutes ...
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bioRxiv - Systems Biology 2023Quote: ... The resulting amplicon was cut from a 1 % agarose gel and purified using the QIAGEN PCR purification kit (QIAGEN, Hilden, Germany). Afterwards ...