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Citations for Qiagen :
1851 - 1900 of 3161 citations for 1 1' 3' 1'' Terphenyl 4 4'' dimethanamine 5' 4 aminomethyl phenyl 9CI since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2024Quote: ... Lysates were titered and 1 mL was allocated for DNA extraction using DNeasy Blood & Tissue Kit (Qiagen catalog number 69504). qPCR was performed in triplicate on 1:10 dilutions of the extracted DNA with TaqMan Real-Time PCR Master Mix ...
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bioRxiv - Microbiology 2024Quote: ... The effect of phage on the key QSS was quantified in real-time using 1 µL of the cDNA with QuantiTect SYBR Green PCR Kit (Qiagen) following the manufacturer’s instructions ...
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bioRxiv - Microbiology 2024Quote: ... Faeces was collected in pre-weighed tubes containing 1 ml PBS and homogenised with a steel ball for 2 minutes at 25 Hz using a Tissue-Lyser (Qiagen). Mice were euthanised at indicated time-points ...
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bioRxiv - Microbiology 2024Quote: ... Cell lysis was performed by bead beating at 50 Hz for 1 min using a TissueLyser LT (QIAGEN, Hilden, Germany). Sequencing was performed by Macrogen Inc ...
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bioRxiv - Immunology 2024Quote: ... Illumina sequencing-compatible Combinatorial Dual Index (CDI) adapters were ligated onto the pools using the QIAseq 1-step amplicon library kit (Qiagen). Library QC was performed using Qubit and Tapestation and sequenced on Illumina MiSeq platform to generate 2×250bp paired-end reads.
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bioRxiv - Immunology 2024Quote: Total RNA was isolated from FACs-sorted PFA-fixed cells (populations as shown in Supplementary Fig. 1) using the miRNAeasy FFPE kit for microRNA extraction (Qiagen). Total RNA integrity was assessed using the Agilent Bioanalyzer ...
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bioRxiv - Genomics 2024Quote: ... Bxb1 integrase-edited Rep 1 and Rep 2 K562attB gDNA genomic DNA was extracted using DNeasy blood and tissue kit (Qiagen) and samples library preparation and Illumina short read sequencing with a target of 60x genomic coverage ...
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bioRxiv - Immunology 2024Quote: Cell-free HIV-1 RNA was isolated from 50 μl plasma using a QIAamp Viral RNA Mini Kit (QIAGEN, 52906) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2024Quote: ... PCR product was treated with 40U Dpn I for 1 hour and was purified by QIAquick PCR Purification Kit (QIAGEN). The purified DNA was quantified by Qubit dsDNA BR kit (Invitrogen).
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bioRxiv - Molecular Biology 2024Quote: ... The reaction mixture was incubated at 37°C for 1 hour followed by purification using QIAquick PCR Purification Kit (QIAGEN). The purified DNA was quantified by Qubit dsDNA BR kit (Invitrogen).
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bioRxiv - Developmental Biology 2024Quote: ... 5 µl of each PCR product was run in a 1% agarose gel and the remaining 35 µls were purified using the QIAquick PCR Purification Kit (Qiagen) before being sent for sequencing (sequencing primers ...
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bioRxiv - Microbiology 2024Quote: ... A 1 μg of total RNA of each sample was reverse-transcribed to cDNA using RT2 first strand kit (Qiagen) according to manufacturer’s protocol ...
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bioRxiv - Immunology 2024Quote: ... semimature SP thymocytes were isolated on BD FACSAria II and BD FACSAria Fusion cell sorters directly into RLT + 1% beta-mercaptoethanol (BME) buffer (Qiagen). Libraries were generated by the Emory Integrated Genomics Core (EIGC ...
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bioRxiv - Microbiology 2024Quote: ... The PCR products were separated on a 1% agarose gel and bands were excised using a QIAquick gel extraction kit (Qiagen). The purified PCR bands were cloned using the NEB PCR cloning kit following manufacturer’s instructions and transformed colonies were screened using colony PCR ...
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bioRxiv - Microbiology 2024Quote: ... and 0.5 mm (0.3 g) Zirconia beads and 1 mL of InhibitEx buffer from the QIAmp Fast DNA Stool Mini Kit (QIAGEN #51604) in a 2 mL tube ...
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bioRxiv - Molecular Biology 2024Quote: ... and HET(2)) and four biological replicates of PA-1 (WT and HET) cells was extracted using miRNeasy Mini Kit (Qiagen) followed by on-column DNAse digestion ...
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bioRxiv - Microbiology 2024Quote: ... Cells were washed twice with 1× PBS and total DNA was extracted at 6 hpi with a DNeasy Blood/Tissue DNA mini kit (Qiagen) and total DNA yield was measured by a nanodrop spectrophotometer (Thermo Fisher Scientific) ...
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bioRxiv - Microbiology 2024Quote: Infected whole eyes were homogenized in 1 mL of sterile PBS using a Retsch Mixer Mill MM300 (Qiagen, Valencia, CA) at 33 Hz for 4 min ...
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bioRxiv - Plant Biology 2024Quote: ... Reverse transcription of 1–2 μg of RNA for cDNA synthesis was carried out using the Omniscript RT Kit (Qiagen).
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bioRxiv - Neuroscience 2024Quote: Total RNA from isolated astrocytes or newborn neurons and MSP-1 differentiation induced astrocytes was isolated using RNeasy Mini Kit (Qiagen).1ng (isolated astrocytes and newborn neurons ...
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bioRxiv - Physiology 2024Quote: ... The digest was run on an 1% Agarose gel (TAE buffer, 0.25 µg/ml EtBr) and the the QIAquick Gel extraction kit (Qiagen, 28704) was used to extract the pLV6 backbone according to the manufacturer’s instructions ...
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bioRxiv - Genomics 2024Quote: ... Cells were briefly vortexed for 1 minute and homogenized by loading the lysate into a QIAshredder spin column (QIAGEN, #79656) and centrifuging for 2 minutes at full speed ...
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bioRxiv - Neuroscience 2024Quote: ... After combining the two aqueous layers 1 equal volume 70% EtOH was added and the solution was added to an RNAeasy column (QIAgen). The RNA was washed with RW1 buffer ...
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bioRxiv - Microbiology 2024Quote: ... 0.5 ml of phenol:chloroform:isoamyl alcohol (25:24:1) was added and samples were bead beaten using a TissueLyser LT (Qiagen, Hilden, Germany) for 10 minutes at 25 Hz ...
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bioRxiv - Cancer Biology 2024Quote: ... we isolated genomic DNA from 2x106 cells (ensuring an average of 1 000x representation for each gRNA) with the AllPrep DNA/RNA Mini Kit (Qiagen), followed by PCR amplification of the gRNAs using forward (5’-CGATACAAGGCTGTTAGAGAGATA-3’ ...
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bioRxiv - Plant Biology 2024Quote: Total RNA was isolated from root tissue 1 cm above the root tip using the RNeasy plant mini kit (Qiagen). Extracted RNA was DNase treated and converted into cDNA with LunaScript RT SuperMix kit (New England Biolabs) ...
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bioRxiv - Biochemistry 2024Quote: ... Nanobody library DNA fragments were digested with the restriction enzymes FseI and Nsil at 37°C for 1 hour and purified using gel extraction (Qiagen). Similarly ...
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bioRxiv - Genomics 2024Quote: ... and the samples from the Democratic Republic of Congo (n = 1) were processed using the QIAsymphony DSP Virus/Pathogen Kit (QIAGEN) with modifications ...
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bioRxiv - Cancer Biology 2024Quote: 1×106 cells were washed with ice-cold PBS and total RNA was extracted using RNeasy kit (Qiagen, Cat#74106). 1 µg RNA was used for the reverse transcription using High-Capacity cDNA Reverse Transcription Kit (Thermo Fisher Scientific ...
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Single-molecule analysis reveals the mechanism of chromatin ubiquitylation by variant PRC1 complexesbioRxiv - Biophysics 2024Quote: ... using 600 units of enzyme in 1 ml total volume at 37 °C for 16-24 hours and purified using QIAquick PCR purification columns (QIAGEN). For ligation ...
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bioRxiv - Systems Biology 2024Quote: ... 1 ml pipette tip connected via a rubber hose to a benchtop air pressure valve with 1 ml of lysis buffer AP1 (Qiagen). Following this ...
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bioRxiv - Cancer Biology 2024Quote: Approximately 0.1 g of mouse fecal pellets were homogenized and processed for DNA extraction using the PowerSoil DNA Isolation Kit (Qiagen) with the QIACube automated platform ...
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bioRxiv - Cell Biology 2024Quote: ... The lysate was then incubated with proteinase K (600 mAU/ml, diluted 1:60 in RNase-free water; Qiagen, Germany) to digest protein ...
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bioRxiv - Developmental Biology 2024Quote: ... The amplified HmlΔ promoter and digested ELAV-GeneSwitch plasmid were each run on a 1% (w/v) agarose TAE gel at 80 V for 1 hour and gel purified using the QIAquick Gel Extraction Kit (QIAGEN). Ligation of the insert and backbone was undertaken at 4°C for 16 hours followed by room temperature (22°C ...
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bioRxiv - Developmental Biology 2024Quote: ... The membrane was then hybridized with 50 pmol mL−1 Biotin-labeled Locked Nucleic Acid-modified DNA probes (designed and synthesized by Qiagen) specific to the 5’ end of tRNA-ValCAC and 5S rRNA ...
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bioRxiv - Cell Biology 2024Quote: For optimisation of pericyte growth conditions RNA was collected from pericytes seeded at 1×105 in a 12-well plate using the RNeasy Mini Kit (74104, Qiagen) after 24 h in the appropriate GA medium ...
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bioRxiv - Cancer Biology 2024Quote: ... and NT5E KO (EV-, D32N-, G34R-, S37F-transfected) HEC-1-A cells was performed using QIAshredder and RNAeasy Mini Kits (QIAGEN) following manufacturer instructions ...
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bioRxiv - Cancer Biology 2024Quote: ... The spheres were washed twice and stained with 1:500 PI solution (Bio-Sciences) supplemented with 250 μg/mL RNAse A (Qiagen) and Hoechst nuclear counterstain (2 μg/mL ...
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Plasma Exosomes in Insulin Resistant Obesity Exacerbate Progression of Triple Negative Breast CancerbioRxiv - Cancer Biology 2024Quote: ... 1 μg of RNA was used to prepare 20 μL of cDNA using the QuantiTect Reverse Transcription Kit (Qiagen, 205313). The Mouse RT2 Profiler™ PCR Array for Epithelial to Mesenchymal Transition (EMT ...
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bioRxiv - Genetics 2024Quote: ... 20 µM of Illumina primers and 1:2000 dilution of 2 ng/µl library on Rotor-Gene Q machines (Qiagen), program and primer sequences are in Table) ...
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bioRxiv - Genetics 2024Quote: ... 10 µM of primers and diluted DNA (1:50 dilution in nuclease free water) from input and immunoprecipitated (IP) fraction using Rotor-Gene Q machines (Qiagen). The qPCR program details and the primers used for qPCR validation are given below:
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bioRxiv - Genetics 2024Quote: ... RNA was isolated from cultures incubated or not with 1 µM staurosporine for 6 hours to induce apoptosis using the RNeasy Mini Kit (QIAGEN). RNA samples were checked for quality on Agilent 4200 TapeStation System (Agilent Technologies ...
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bioRxiv - Physiology 2022Quote: ... Fifteen milligrams of liver were homogenized in 200 µL of a 3:3:2 solution of acetonitrile:isopropanol:water (MeCN:IPA:H2O) in ceramic bead tubes (1.4 mm, Qiagen #13113-50) using a TissueLyzer II (Qiagen #85300) ...
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bioRxiv - Synthetic Biology 2021Quote: ... PCR products were analysed by gel electrophoresis using 1% agarose gel and purified using QIAquick gel extraction kit (Qiagen, Hilden, Germany). The DNA concentrations of the gel-purified samples were quantified with Nanodrop OneC (Thermo Fisher Scientific ...
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bioRxiv - Cancer Biology 2021Quote: ... Real-time PCR was performed with 1:2 diluted cDNA using SYBR green detection system (Thermo Fischer Scientific, F415L) and Rotorgene Q (Qiagen, 9001560). 18S rRNA gene was used as internal control for normalization ...
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bioRxiv - Cell Biology 2020Quote: ... Amplicons were electrophoresed in 1% agarose gel to check for amplification and the size distribution of the library and then column purified (Qiagen, 28104). Once purified ...
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bioRxiv - Microbiology 2021Quote: ... Each gene segment was amplified using segment-specific primers and extracted from 1% gel slices using Qiagen Gel Extraction Kit (Qiagen, Germany). Purified gene segments were cloned into the plasmid pHWSccdB as previously done (27) ...
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bioRxiv - Developmental Biology 2020Quote: ... 1 μg total RNA was isolated from P0 Hnf4a mutant and control kidneys using the RNeasy Plus Micro kit (Qiagen 74034) followed by mRNA was isolation with NEBNext Poly(A ...
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bioRxiv - Plant Biology 2020Quote: ... Aliquots of RNA (1 µg) from each sample were reverse transcribed to cDNA using the QuantiTect® Reverse Transcription Kit (QIAGEN), according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2021Quote: ... The amplified products were analyzed onto 1% agarose gel (low EEO) and extracted and purified from the gel using the QIAprep Miniprep Kit (Qiagen, Netherlands). The 16S rRNA sequencing reactions were performed using 785F (5’-GGATTAGATACCCTGGTA-3’ ...