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Citations for Qiagen :
1851 - 1900 of 10000+ citations for Mouse T Cell Surface Glycoprotein CD8 Beta Chain CD8B ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Bioengineering 2020Quote: ... A Qiagen RT2 SYBR Green master mix with validated qPCR human primers (for HUVECs and BeWo) or mouse primers (for N27 cells) from Qiagen (Frederick) were used to determine relative magnitudes of gene-expression levels using RT-PCR ...
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bioRxiv - Bioengineering 2023Quote: Total RNA from cultured cells and mouse Achilles tendons were isolated with TRIzol reagent and RNeasy Mini Spin Column (Qiagen Sciences) and reversely transcribed into cDNAs using a SuperScript IV VILO Master Mix (Life Technologies).22,30 The relative abundances of genes of interest were determined by TaqMan PCR using primers and probes purchased from Applied Biosystems TaqMan Gene Expression Assays ...
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bioRxiv - Microbiology 2020Quote: ... RNAlater was removed from the sorted cells prior to RNA isolation and total RNA was isolated from the pelleted cells using the RNeasy mini kit (Qiagen, Hilden, Germany) following the manufacturer’s instructions with some modifications ...
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bioRxiv - Microbiology 2020Quote: ... RNA from cell lysates or cell culture supernatants was extracted using the Qiagen QIAamp viral RNA mini kit (Qiagen, Valencia, CA, USA). Reverse transcription (RT ...
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bioRxiv - Cancer Biology 2019Quote: ... RNA and DNA was extracted from the same cells from neuropathologist annotated tumour regions (>60% cancer cells) using appropriate Qiagen kits (Qiagen, Sussex, UK). Paired end strand-specific whole transcriptome libraries were prepared for 16 pairs using the NEBNext Ultra Directional RNA Library Prep Kit for Illumina (New England BioLabs ...
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bioRxiv - Cancer Biology 2020Quote: DNA coming from CD34+ cells of the patient and Mononuclear cells of Healthy Donors was extracted by means of DNeasy Blood & Tissue Kit (Qiagen, Hilden, Germany). 50ng of DNA were analysed through P056 MLPA kits (MRC Holland ...
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bioRxiv - Cell Biology 2021Quote: ... Genomic DNA was extracted from the control and the peptide-treated frozen cells using the Blood & Cell Culture DNA Midi Kit according to manufacturer’s instructions (Qiagen, ref. no. 13343). A first PCR was performed to amplify the lentiCRISPR sgRNA region using the following primers:
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bioRxiv - Cancer Biology 2020Quote: ... Genomic DNA was extracted from osimertinib-resistant cells as well as matched parental cells were extracted using a DNeasy Blood & Tissue Kit (Qiagen, Hilden, Germany). We reviewed all candidate alterations identified in resistant cells as well as parental cells and considered those identified only in resistant cells as candidate acquired resistance genomic alterations to osimertinib.
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bioRxiv - Microbiology 2022Quote: RNA was extracted from purified CD4+ TN and TCM cells and from 24ST1NLESG cells using the RNeasy mini kit (Qiagen, Valencia, CA) according to the manufacturer’s protocol ...
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bioRxiv - Biochemistry 2023Quote: ... gDNA was isolated from 5 x 106 Colo205 cells using the Blood and Cell Culture DNA Maxi Kit (Qiagen, catalog no. 13362) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2023Quote: gDNA samples from the unselected control (gDNACtrl) and 2nd (gDNAScreen) selected group cells were extracted using the Blood and Cell Culture DNA Midi Kit (#13343; QIAGEN, Germantown, MD).
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bioRxiv - Neuroscience 2024Quote: Total RNA was extracted from the SBMA OL cell model and the control cell model using the RNeasy Mini Kit (Qiagen, Hilden, Germany). The quality and quantity of the RNA obtained were checked using a NanoDrop 2000 spectrophotometer (Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2021Quote: ... and transfected with 1 μg of a pcDNA3.1 vector containing human αSyn cDNA (provided by T. Baron, Anses, Lyon, France) using Effecten transfection reagent (Qiagen). Positive clones were selected using geneticin and cultured in Dulbecco’s modified Eagle’s medium (DMEM/F-12 ...
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bioRxiv - Developmental Biology 2020Quote: ... the allantois was dissected out and lysed in 10 μl DirectPCR (Tail) digest reagent (Qiagen, catalog no. 102-T) supplemented with 1 μl of proteinase K overnight at 55°C ...
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bioRxiv - Biophysics 2023Quote: ... The α1(L9’T) and αm mutations were introduced individually or serially by site-specific mutagenesis (QuikChange II, Qiagen). Mutations βm and γm were obtained from GENEWIZTM ...
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bioRxiv - Biochemistry 2023Quote: The coding sequence of mouse Donson was amplified from XpressRef Universal Total mouse RNA (QIAGEN, 338114) by RT-PCR (Takara ...
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bioRxiv - Cancer Biology 2021Quote: ... The cell pellet was processed for total RNA isolation using an RNeasy Mini Kit (Qiagen).
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bioRxiv - Cancer Biology 2021Quote: Total mRNA was extracted from frozen cell pellets using the RNeasy Extraction Kit (Qiagen 74136). Reverse transcription and qPCR was performed using the iTaqTM Universal SYBR® Green 1-Step Kit (BioRad 1725151 ...
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bioRxiv - Neuroscience 2021Quote: Cells were harvested at DIV7 or DIV11 in buffer RLT (RNeasy RNA isolation kit, Qiagen) for total RNA extraction according to manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2021Quote: ... Total RNA was extracted from cells using a RNeasy Plus RNA extraction kit (Qiagen, 74136). An on-column DNase digest (Qiagen ...
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bioRxiv - Cancer Biology 2021Quote: Total RNA was extracted from cells using a RNeasy Plus RNA extraction kit (Qiagen, 74136) according to the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2020Quote: Total RNA was extracted from the specified cell lines using the RNeasy Mini Kit (Qiagen) with DNase treatment ...
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bioRxiv - Cell Biology 2020Quote: ... subsequently RNA was isolated from the cells using the RNeasy micro kit (Qiagen, Hilden, Germany) according to manufactures’ instructions ...
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bioRxiv - Cell Biology 2020Quote: RNA was extracted from pelleted cells using either an RNeasy Mini kit and QIAshredder (QIAGEN) or a Direct-zol RNA Miniprep kit (Zymo Research ...
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bioRxiv - Cell Biology 2020Quote: ... DNA was prepared using a Qiagen Blood and Cell Culture DNA Kit (Qiagen, Venlo, Netherlands). 15-20 µg DNA was added to 124 µL of 2M CaCl2 and the total volume brought to 1 mL ...
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bioRxiv - Cell Biology 2020Quote: ... DNA was extracted from 80 x106 cells using the QIAamp DNA Blood Maxi Kit (QIAGEN). sgRNA inserts were PCR amplified using ExTaq DNA Polymerase (Takara) ...
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bioRxiv - Cell Biology 2020Quote: RNA was extracted from cells using the RNeasy kit according to the manufacturer’s instructions (Qiagen) and RNA reverse transcribed using the SuperScript III Reverse Transcriptase (Thermo Fisher Scientific) ...
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bioRxiv - Cell Biology 2020Quote: RNA from 2×107 cells was isolated using the RNeasy Mini Kit (Qiagen, Venlo, Netherlands), cDNA was generated by reverse transcription on CFX96 real-time system (BioRad ...
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bioRxiv - Immunology 2021Quote: RNA was extracted from SZ95 sebocyte cells using the RNAeasy Plus universal kit (Qiagen 73404). RNA quality was assessed using Agilent 2100 Bioanalyzer ...
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bioRxiv - Immunology 2021Quote: ... The cells were harvested and the RNA was prepared using RNeasy Mini Kit (Qiagen, Germany) as per the manufacturers protocol ...
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bioRxiv - Genetics 2021Quote: ... and the DNA in the pellets was amplified with REPLI-g Single Cell kit (QIAGEN) for sequencing on the MiSeq or HiSeq 3000 sequencing system (Illumina).
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bioRxiv - Genetics 2021Quote: RNA from sorted microglial cells was isolated using the miRNeasy Serum/Plasma Kit (QIAGEN; 217184) and quantified on the Agilent BioAnalyzer 2100 ...
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bioRxiv - Cancer Biology 2020Quote: Genomic DNA was extracted from ccRCC cells using the DNeasy Blood and Tissue Kit (Qiagen). Epitect Methyl II PCR Array System (Qiagen ...
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bioRxiv - Developmental Biology 2020Quote: Total RNA was prepared from sorted or dissociated cells with an RNeasy Mini Kit (Qiagen) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2022Quote: ... All plasmids for mammalian cell experiments were purified using the Plasmid Plus Midi Kit (Qiagen) or the Spin Miniprep Kit (Qiagen) ...
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bioRxiv - Cancer Biology 2022Quote: Total RNA was extracted from cell lines or frozen tumour samples using RNeasy kit (Qiagen). Frozen tumour samples were homogenised prior to RNA extraction either using a syringe and needle or QIAshredder columns (Qiagen) ...
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bioRxiv - Neuroscience 2021Quote: Total RNA from cultured cells was isolated with Qiagen RNeasy mini extraction kit (Qiagen, #74106) following the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2020Quote: ... cells were harvested and RNA was extracted using RNeasy Plus Mini Kit (Qiagen, Hilden, Germany). Subsequently ...
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bioRxiv - Molecular Biology 2021Quote: ... Genomic DNA from mESC founder lines was extracted with Gentra Puregene Cell Kit (Qiagen, 158745) following the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2022Quote: ... gDNA was extracted from Cas9-mgRNA cells using Qiagen DNeasy Blood & Tissue kit (Qiagen #69506). The final product was eluted in 60 uL of elution buffer and quantified using QuBit (Thermo) ...
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bioRxiv - Immunology 2022Quote: RNA transcripts were isolated from cells using a Qiagen RNEasy Mini RNA isolation kit (Qiagen) per the manufacturer’s instructions ...
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bioRxiv - Genomics 2020Quote: Total RNA was extracted from 1–2 million cells using the AllPrep Mini kit (QIAGEN) according to the manufacturer’s instructions and 1 μg of total RNA was used to prepare each RNA-seq library ...
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bioRxiv - Genomics 2020Quote: RNA was purified from approximately 1 × 107 CHM13 cells using an RNeasy kit (Qiagen; 74104) and prepared into Iso-Seq libraries following a standard protocol68 ...
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bioRxiv - Genomics 2020Quote: ... S2 cells were transfected with 1 ug plasmid DNA using the Effectene reagent kit (Qiagen). The plasmid DNA which contains cDNA of FLAG-tagged ECDs are under the metallothionein promoter control ...
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bioRxiv - Genomics 2020Quote: ... Genomic DNA was extracted and purified from cells using QIAamp DNA Micro Kit (Qiagen 56304) with the user-developed protocol QA43 and no more than ~0.5M cells per column ...
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bioRxiv - Molecular Biology 2021Quote: RNA was extracted from the induced harvested cells using the RNeasy Plus Mini Kit (Qiagen). This RNA was used for qPCR to validate the RBP knockdown as well as for semi-quantitative RT-PCR experiments to check the splicing pattern of endogenous CD19 ...
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bioRxiv - Genomics 2019Quote: ... gDNA from PC3 and DU145 cells were purified using the QIAamp DNA Mini Kit (Qiagen) and sonicated to ~170 bp using a Covaris S2 sonicator to create a synthetic DNA template with similar physical properties to cfDNA.
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bioRxiv - Cancer Biology 2019Quote: Melanoma cell lines were collected and RNA isolated with the RNeasy kit (Qiagen, Valencia, CA). After subsequent cDNA amplification using Superscript III (Invitrogen ...
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bioRxiv - Genetics 2019Quote: DNA from whole cell populations was purified using Qiagen DNeasy Blood & Tissue Kit (Qiagen 69504). PCR amplification was performed with MyTaq DNA Polymerase (Bioline BIO-21108 ...
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bioRxiv - Microbiology 2020Quote: Viral RNA was extracted from cell culture supernatants using QIAamp Viral RNA Mini Kit (Qiagen) and reverse-transcripted to cDNA using LunaScript RT SuperMix kit (New England Biolabs ...