Labshake search
Citations for Qiagen :
1751 - 1800 of 10000+ citations for Mouse Marginal Zone B And B1 Cell Specific Protein MZB1 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2024Quote: Vector genome copy number was quantified by qPCR analysis of total DNA extracted from mouse tissues using Qiagen DNeasy Blood and Tissue kit and Tissue Lyser II (Qiagen, Hilden, Germany). Genomic RNA was eliminated by RNase digestion (Qiagen ...
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bioRxiv - Neuroscience 2024Quote: Eye tissue was suspended in Buffer RLT/eye (1 eye/mouse; 10-18 mice/genotype) and prepared using the RNeasy kit (Qiagen, Germantown, MD). RNA quality was assessed by the 18S/28S rRNA bands in capillary electrophoresis (Bioanalyzer ...
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bioRxiv - Developmental Biology 2022Quote: ... SPACA6 concentrated to 7 mg mL−1 in Buffer B was mixed in a 1:1 volumetric ratio (0.3:0.3 mL) with JCSG+ (Qiagen), Cryos (Qiagen) ...
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bioRxiv - Molecular Biology 2023Quote: Inhibitors (antimiRs) against miR26b-5p and miR200a/b/c-3p were purchased from Qiagen (339130 and 339160 respectively). AntimiRs in injection media (1mM Tris-HCL pH 7.5 and 0.5 mM EDTA in embryo grade water ...
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bioRxiv - Immunology 2022Quote: ... Three hundred cells from three to six mouse per condition were sorted directly into 25 μl TCL buffer (Qiagen, 1031576) supplemented with 1%β-mercaptoethanol at single cell precision using a BD FACS Aria II instrument ...
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bioRxiv - Immunology 2021Quote: ... Total RNA from the cells and EVs were first isolated and purified using an RNeasy Mini Kit and a miRNeasy Serum/Plasma kit (Qiagen, Hilden, Germany), respectively ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: RNA was extracted from clarified cell culture supernatants (16,000 g x 10 min) and from infected cells using QIAamp Viral RNA Mini Kit and RNeasy Plus mini kit (Qiagen, Hilden, Germany), respectively ...
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bioRxiv - Genetics 2021Quote: ... and that of cultured cells was obtained using the AllPrep DNA/RNA/miRNA Universal kit or the miRNeasy mini kit (QIAGEN, Hilden, Germany). The samples were reverse-transcribed into cDNA with the High-Capacity cDNA reverse transcription kit (Thermo Fisher Scientific).
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bioRxiv - Bioengineering 2022Quote: ... Total RNA from the cells and EVs was isolated and purified using an RNeasy Mini Kit and a miRNeasy Serum/Plasma kit (Qiagen, Hilden, Germany), respectively ...
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A conserved isoleucine in the binding pocket of RIG-I controls immune tolerance to mitochondrial RNAbioRxiv - Immunology 2022Quote: ... was extracted from whole cell RNA extracts (total RNA) using the miRNeasy Mini Kit and the RNeasy MiniElute Cleanup Kit (both Qiagen, Hilden, Germany). Extraction was performed according to the manufactureŕs instructions.
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bioRxiv - Microbiology 2023Quote: ... Clarified cell lysate was processed using a Qiagen RNeasy kit and in-column treatment with a DNase I kit (Qiagen, Germantown, MD) for purification of total RNA ...
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bioRxiv - Genomics 2022Quote: Total RNA was extracted from Min6 cells using RNeasy Plus mini kit and from pancreatic islets using RNeasy Plus micro kit (Qiagen, ref 74034) following the manufacturer’s instruction ...
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bioRxiv - Neuroscience 2023Quote: ... The left hippocampus was homogenized on ice in the lysis buffer from the AllPrep® DNA/RNA/Protein Mini Kit (QIAGEN, Hilden, Germany). A sample from the lysate was used to quantify total DNA and RNA content using the appropriate Qubit® Fluorometer kit (Cat ...
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bioRxiv - Cell Biology 2019Quote: Genomic DNA from 60 million unsorted cells (500× coverage of the GeCKO v2 library) and 6 million sorted cells was isolated using Blood & Cell Culture DNA Midi Kit (QIAGEN, 13343) according to the manufacturer’s instructions ...
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bioRxiv - Synthetic Biology 2019Quote: ... total RNA was extracted from cells from stable-cell lines created using a RNeasy Plus Mini Kit (Qiagen, catalog number: 74134) following the Manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2021Quote: ... Cell pellets were then rinsed with 1X PBS and processed for subcellular fractionation (Qproteome Cell Compartment Kit, Qiagen; following manufacturer’s protocol). Nuclear fractions were isolated and protein precipitated by incubation in 4x volume of ice-cold acetone for 15 minutes followed by pelleting (10 min ...
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bioRxiv - Microbiology 2020Quote: Total cellular DNA was extracted from cells (cell lines or PBMCs) using a QIAmp DNA Blood mini kit (Qiagen, Hilden, Germany) according to manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2021Quote: ... Total RNA from untreated cells (24 h) and IFNγ-treated cells (6 or 24 h) were extracted with RNeasy plus mini kit (Qiagen, 74134) according to manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2020Quote: Total RNA was extracted for each of the persistently infected cells as well as the cell lines without NDV infection (negative control) using Qiagen RNeasy micro kit (Qiagen, Germany) following the manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2022Quote: Cell lysis and RNA extraction from siRNA transfected cells were performed using the RNeasy RNA extraction kit (Qiagen Inc, Germantown, MD). 1 µg of total RNA was reverse transcribed with the High-capacity RNA-to-cDNA kit (Applied Biosystems ...
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bioRxiv - Neuroscience 2022Quote: E3 and E4 primary microglia were plated at 5×106 cells/well in 6-well plates and RNA was extracted from the cells using the RNEasy Plus Mini Kit (Qiagen #74136) and converted to cDNA using High-Capacity RNA-to-cDNA kit (Thermo #4387406 ...
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bioRxiv - Genomics 2019Quote: ... and the remaining cells in single-cell capture (T1, T2, and T3 samples) using the QIAamp DNA Mini kit (Qiagen, 51304), and 500 ng of DNA were used to construct exome sequencing libraries with the same reagents as the single cell DNA-seq.
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bioRxiv - Genomics 2021Quote: ... the cells were collected and total RNA was extracted from the cells using the Qiagen RNeasy® Plus Kit (Qiagen, 74134). One µg of RNA was then used to synthesize cDNA using the Bio-RAD iScript cDNA Synthesis Kit (Bio-RAD ...
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bioRxiv - Cell Biology 2019Quote: ... MCMBP depleted cells using siRNA and MCMBP-KO (clone #1 and #2) cells was isolated using RNeasy Mini Kit (Qiagen, 74104). The cDNA was synthesized using High-Capacity cDNA Reverse Transcription Kit (Thermo Fischer Scientific ...
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bioRxiv - Evolutionary Biology 2019Quote: ... A pellet of 200-400 µl of animals was resuspended in 400 µl Cell Lysis Solution (Qiagen Gentra Puregen Cell kit) with 5 µl proteinase K (20 mg/ml ...
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bioRxiv - Evolutionary Biology 2022Quote: ... log-phase cells in the range of 1–2 × 108 cells using the Qiagen DNeasy® Blood and Tissue kit (Qiagen). We resuspended the genomic DNA in 10 mM Tris-HCl pH 8.5 and stored it at 4° until submission to the McDonnell Genome Institute at Washington University in St ...
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bioRxiv - Cancer Biology 2024Quote: Total RNA was extracted from CD33+ ITGA7-sorted primary AML cells (> 4x105 cells/sample) using the RNeasy Mini kit (Qiagen, 74106) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2023Quote: Total RNA from 1,000 H3K27me3 HI/LO β-cells or from 50,000 sorted βHI and βLO cells was extracted using the miRNeasy FFPE Kit (QIAGEN, 217504), followed by the NEBNext® Single Cell/Low Input RNA Library Prep Kit for Illumina® (E6420L) ...
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bioRxiv - Molecular Biology 2024Quote: ... ∼5×107 live cells were harvested per replicate and cell pellets underwent same-day nuclei preparation using Qiagen Blood & Cell Culture DNA Maxi Kit (Qiagen, #13362) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2021Quote: ARNO siRNA (Mm_Pscd2_3) or a negative non-specific siRNA control (Allstar siRNA) were transfected into SFs using HiPerFect transfection reagent (all Qiagen,UK) according to the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2021Quote: ... quantitative RT-PCR, and immunoblotting. For knock-down experiments, non-specific (siNS, No. 1027310) or AHR (siAHR, SI02780148) siRNA (QIAGEN, Inc.) was transfected with LipofectamineTM RNAiMAX (Thermo Fisher Scientific Inc. ...
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bioRxiv - Cell Biology 2019Quote: ... 25-50ng cDNA and 10 pmol gene specific primer were used in a 10 μl SYBR green reaction mix (Qiagen, 204056) for Quantitative RT-PCR (Supplemental Table 1 ...
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bioRxiv - Cancer Biology 2021Quote: ... Total mRNA were extracted from frozen cell pellets using RNeasy Extraction Kit (Qiagen 74136) and submitted to the Nationwide Children’s Hospital Institute for Genomic Medicine for RNA quality measurements (RIN and DV200) ...
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bioRxiv - Developmental Biology 2021Quote: RNA was extracted from frozen cell pellets using the DNA/RNA mini kit (Qiagen) and subsequently DNase treated using the TURBO DNA-free kit ‘Rigorous DNase treatment’ protocol ...
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bioRxiv - Developmental Biology 2021Quote: ... RNA was extracted from cells with the RNeasy Micro Kit (Qiagen, Catalog No. 74004) using the Qiacube automated system (Qiagen) ...
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bioRxiv - Cell Biology 2020Quote: ... DNA was isolated from cell pellets using the DNeasy Blood & Tissue kit (69505, Qiagen). Primers were generated to amplify the surrounding region of the guide RNA target sites with the following primer pairs ...
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bioRxiv - Cell Biology 2020Quote: ... RNA was extracted from collected cells using the Qiagen RNeasy Plus Mini Kit (Qiagen) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2020Quote: Mitochondria from 107 HeLa cells were extracted using a Qproteome Mitochondria Isolation Kit (Qiagen). After the final wash with mitochondria storage buffer ...
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bioRxiv - Cell Biology 2020Quote: RNA from Flp-In™293 cells was extracted using the RNeasy kit (Qiagen) in accordance with the manufacturer’s instructions that included the genomic DNA digestion with DNaseI (Qiagen) ...
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bioRxiv - Cell Biology 2020Quote: ... and run on the RT^2 First Strand Kit (Stem cell PCR array) (Qiagen). The array was run in triplicate (N=3 ...
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bioRxiv - Immunology 2021Quote: ... RNA was extracted from NK cells using the RNeasy Mini Kit (Qiagen, Hilden, Germany) and RNAseq was performed (Supplemental Methods) ...
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bioRxiv - Immunology 2021Quote: Cell and lung tissue RNA was extracted with RNeasy Miniprep Kit (QIAGEN, Cat# 74136), in accordance with the manufacturer’s instruction ...
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bioRxiv - Immunology 2021Quote: Total RNA from cells was extracted using the RNeasy Mini Kit (Qiagen, Hilden, Germany), and residual DNA contaminations were removed with the RNase-free DNase set (Qiagen ...
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bioRxiv - Genetics 2021Quote: ... All plasmids used for mammalian cell experiments were purified using plasmid Miniprep kits (Qiagen).
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bioRxiv - Genetics 2021Quote: ... Plasmid DNA was extracted from competent cells with the QIAprep Spin Miniprep Kit (Qiagen) and linearized with Xmal ...
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bioRxiv - Immunology 2020Quote: Total RNA was purified from FACS sorted cells using the RNeasy Micro kit (Qiagen). RNA quality was assessed with an Agilent 2100 Bioanalyzer (RNA integrity number>9 for all samples) ...
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bioRxiv - Molecular Biology 2022Quote: RNA was isolated from 1×107 cells with the RNeasy® Mini Kit (Qiagen) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2019Quote: RNA was extracted from three independent cell populations using the RNeasy Mini Kit (QIAGEN), according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2021Quote: ... RNA was extracted from granulosa cells using RNeasy Mini kit (Qiagen, Chadstone, VIC, Australia). RNA quality was assessed using the RNA ScreenTape System (Agilent ...
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bioRxiv - Molecular Biology 2021Quote: ... Total RNA extraction from bacterial cells was performed using an RNeasy mini kit (Qiagen) according to the manufacturer’s instructions ...