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Citations for Qiagen :
1601 - 1650 of 10000+ citations for Mouse Marginal Zone B And B1 Cell Specific Protein MZB1 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2022Quote: DNA was purified for sequencing from cells infected in six well plates using a blood and cell culture DNA mini kit (Qiagen). Samples were sequenced at the Northwestern University Genomics Core Facility using NextGen Illumina HiSeq SR500 sequencing ...
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bioRxiv - Immunology 2023Quote: ... Genomic DNA was extracted from puromycin selected SUDHL4 cells and untreated WT cells using the QIAamp DNA Blood Mini Kit (Qiagen) following the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2022Quote: ... the cell culture supernatants were collected and cells were lysed using RLT Buffer from the RNeasy Mini Kit (#74106; Qiagen).
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bioRxiv - Cell Biology 2022Quote: Genomic DNA was isolated from 1 million cells of each cell line using the QIAamp®DNA Micro Kit (QIAGEN) following the manufacturer’s guidelines ...
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bioRxiv - Biochemistry 2023Quote: ... Mitochondria isolation from HEK293T cells was performed after 24 hours of cell transfection using the Q proteome Mitochondria Isolation Kit (QIAGEN). Cell lysis and mitochondria homogenisation were conducted as per the manufacturer’s instructions ...
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bioRxiv - Pathology 2023Quote: ... RNA from CD4+ T cells (2×106 cells per condition) was extracted by using the RNeasy Plus Mini kit (QIAGEN). Extracted total RNA was quantified using the Qubit broad range RNA assay ...
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bioRxiv - Developmental Biology 2023Quote: ... we used 6 mL of 55 °C Cell Lysis Solution from The Gentra Puregene® Cell and Tissue Kit (Qiagen) with 0.1 mg/mL proteinase K and 1% β-mercaptoethanol ...
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bioRxiv - Molecular Biology 2023Quote: Bulk-RNA free of genomic DNA for RT-qPCR analysis was extracted from cell pellets (≤ 2 x 106 cells) using the RNeasy plus spin-column purification kit (Qiagen) and performed using the Qiacube liquid handler (Qiagen ...
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bioRxiv - Molecular Biology 2023Quote: Genomic DNA from Library-Diverse was isolated by Blood & Cell Culture DNA Maxi Kit (Qiagen; 30 million cells per condition). Genomic DNA from the TRIP library editing experiments was isolated by DNeasy Blood & Tissue Kit (4 million cells per condition) ...
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bioRxiv - Bioengineering 2023Quote: ... phenol-free total RNA was extracted from cells directly in cell culture wells using RNeasy Plus Micro Kit with gDNA eliminator columns (Qiagen) following the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2023Quote: Cells collected from either 3D culture organoids or sorted cells were lysed in RLT buffer from RNeasy kit (Qiagen, 74106). RNA samples were isolated using the same kit following the manufacturer’s recommendations ...
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bioRxiv - Immunology 2023Quote: Genomic DNA from ex vivo isolated CD8+ T cell subsets or in vitro cultured cells was extracted using the DNeasy Blood & Tissue Kits (Qiagen) and subsequently bisulfite-converted by using the EZ DNA Methylation-Lightning Kit (Zymo Research ...
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bioRxiv - Genetics 2023Quote: ... Genomic DNA Extraction and Purification: Genomic DNA was extracted from 5 M cells using the Gentra Puregene Cell Kit (Qiagen) following the manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2024Quote: Genomic DNA from freshly sorted single cells was extracted and amplified using the REPLI-g Advanced Single Cell kit (Qiagen) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2024Quote: The DNA was extracted from the FACS sorted cells and whole genomes were amplified using multiple displacement amplification technique (MDA) with the REPLI-g Single Cell kit (Qiagen) by following the manufacturer instructions ...
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bioRxiv - Cell Biology 2024Quote: ... genomic DNA was extracted from cell pellets containing at least 1e6 cells/pellet using the DNeasy Blood and tissue kit (Qiagen), followed by bisulfite conversion using the Epitech Fast Bisulfite Conversion Kit (Qiagen) ...
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bioRxiv - Microbiology 2019Quote: ... Synthetized cDNA was subjected to a PCR array specific for the human antiviral response (RT² Profiler PCR array – PAHS-122Z, SA Biosciences, Qiagen), according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2022Quote: ... from the significant negatively selected genes to define the “DMG specific dependency genes” and performed pathway analysis on the gene set that was identified as DMG specific dependency in at least two of the DMG lines using the Core Analysis of IPA (QIAGEN Inc. ...
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bioRxiv - Genetics 2022Quote: ... qRT-PCR was performed by QuantiTect SYBR Green PCR using miRNA- specific primers provided as miScript Universal primer and miScript Primer assay (QIAGEN) and Thermal Cycler Dice Real Time System (TAKARA).
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bioRxiv - Genomics 2019Quote: ... For the bacteria-specific 341F/805R47 primer pairs a different reaction mixture was used containing 10x Standard Taq Reaction buffer (Qiagen), 2 mM MgCl2 ...
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bioRxiv - Biochemistry 2019Quote: ... The bisulfite treated DNA was then used for PCR amplification using the amplicon and primers with treatment specific barcodes and HotStarTaq DNA Polymerase (Qiagen). PCR products were resolved on an 1% agarose gel ...
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bioRxiv - Cancer Biology 2021Quote: ... Quantitative real-time PCR (QPCR) using gene-specific primers (supplementary table S9) was performed using QuantiNova SYBR Green mix (Qiagen) on an MX3000P real-time PCR machine (Stratagene) ...
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bioRxiv - Microbiology 2019Quote: ... the coding region of MW1875 or MW1874 amplified by PCR using specific primers was cloned into pQE30 (QIAGEN, Tokyo, Japan) which is used for the construction of histidine-tagged recombinant proteins ...
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bioRxiv - Biochemistry 2021Quote: 200 ng of DNA-free total RNA extracted from replicates was used for gene-specific cDNA synthesis by the MLV-reverse transcriptase (Qiagen). Quantitative (q)-PCRs were performed using the PowerUp SYBR Green Master Mix (ThermoFisher Scientific ...
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bioRxiv - Immunology 2020Quote: ... the miScript Universal Primer was used alongside miR specific miScript primer assays (miR-206 cat. no. MS00001869 and U6 cat. no. MS00033740; Qiagen).
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bioRxiv - Neuroscience 2023Quote: Biological processes and regulatory networks exhibiting diet-specific activation patterns were investigated using Ingenuity Pathway Analysis (IPA; Qiagen, Hilden, Germany). Given the limited number of DEGs identified at a false discovery rate (FDR ...
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bioRxiv - Cancer Biology 2024Quote: ... cDNA generated after performing reverse transcription of RNA product was subjected to quantitative PCR (qPCR) analysis using gene-specific predesigned primers (RT2 qPCR Primer Assay, Qiagen), listed in Supplementary Table S4 ...
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bioRxiv - Biochemistry 2023Quote: ... HBEC5i cells with treated with either non-specific siRNA or Flvcr2 siRNA were harvested and kept in 1 mL TRI reagent (Qiagen).
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bioRxiv - Immunology 2023Quote: ... cDNAs were synthesized using High Capacity RNA-to-cDNA kit (ThermoFischer Scientific) and quantitative RT-PCR were carried out using specific primers (Table S2) and SYBR Green PCR Master Mix (Qiagen). PCR amplification of Gapdh was performed to control for sample loading and normalization between samples ...
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bioRxiv - Molecular Biology 2023Quote: ... or to mature primate-specific miR-608 (AM608, 15-mer, as a control with no predicted complementary sequences in mice) (LNA, Exiqon, Qiagen). DIO mice were injected intravenously with 3.3 mg/kg oligonucleotide for three consecutive days and were sacrificed 0 ...
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bioRxiv - Cell Biology 2022Quote: ... were performed on significantly altered proteins in CAVIN1-null cells using the “core analysis” function included in the Ingenuity Pathway Analysis (IPA) software (QIAGEN Bioinformatics ...
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bioRxiv - Biochemistry 2019Quote: Mutant Chinese hamster BiP proteins with an N-terminal His6-tag were purified as described before with modifications (Preissler et al, 2017b). Proteins were expressed in M15 Escherichia coli (E. coli) cells (Qiagen). The bacterial cultures were grown in LB medium supplemented with 100 µg/ml ampicillin and 50 µg/ml kanamycin at 37 ° C to an OD600nm of 0.8 and expression was induced with 1 mM IPTG ...
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bioRxiv - Bioengineering 2021Quote: The dataset of differentially abundant proteins between groups were classified for cell compartment association with Ingenuity Pathway Analysis (IPA, Qiagen) and for matrisome categories using The Matrisome Project database,[28] ...
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bioRxiv - Microbiology 2022Quote: ... Gene expression of 84 heat shock genes in mock-infected or 229E-infected cells was analyzed using Human Heat Shock Proteins & Chaperones RT2 Profiler PCR array (Qiagen). Real-time PCR analyses were performed with specific primers ...
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bioRxiv - Immunology 2021Quote: ... Samples were then centrifuged and plasma isolated and stored at -20°C prior to testing in the QuantiFERON IFNγ ELISA (Qiagen). Acceptance criteria for the assay specified by manufacturers were always met.
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bioRxiv - Cancer Biology 2022Quote: ... and protein extraction were performed according to manufacturer instructions (AllPrep DNA/RNA/Protein minikit; Qiagen). Each spin column flowthrough (DNA ...
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bioRxiv - Microbiology 2023Quote: ... The His-tagged proteins were purified using the QIAexpress Ni-NTA Protein Purification System (Qiagen) with 0.1 M ...
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bioRxiv - Neuroscience 2022Quote: PCR with forward and reverse primers (see Table 1) was performed on mouse hypothalamic cDNA (isolated with miRNeasy Mini Kit, Qiagen, Hilden, Germany). PCR products were ligated into pGEMT.easy (Promega ...
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bioRxiv - Genomics 2020Quote: ... and AH (20 weeks) old C57/Bl6/J mouse hearts (n= 4-6/group) using the RNeasy Mini Kit (Qiagen, Cat.74106) according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2020Quote: ... Total RNA and miRNA fractions were isolated from the mouse frozen tissue with the miRNeasy Micro Kit protocol (Qiagen, Toronto, ON, Canada). All RNA samples were determined to have 260/280 and 260/230 values ≥1.8 ...
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bioRxiv - Microbiology 2022Quote: ... DNA was extracted and purified from 20 mg (2 pellets) of stool from each mouse using the QIAamp Fast DNA Stool Mini Kit (Qiagen, Germantown, MD). The concentration of DNA in samples was determined by spectrophotometry ...
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bioRxiv - Cancer Biology 2022Quote: Total RNAs from mouse tumors were extracted and purified using the RNeasy Mini Kit and RNase-free DNase Set (QIAGEN, Valencia, CA) following the protocol provided by the manufacturer ...
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bioRxiv - Neuroscience 2021Quote: DNA was isolated from tail biopsies using the Gentra Puregene Mouse Tail Kit according to the manufacturer’s instructions (Qiagen, Valenica, CA, USA). Genomic DNA was digested with BamHI-HF (New England Biolabs ...
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bioRxiv - Neuroscience 2019Quote: ... Total RNA and miRNA fractions were isolated from the mouse frozen tissue with the miRNeasy Micro Kit protocol (Qiagen, Toronto, ON, Canada). All RNA samples were determined to have 260/280 and 260/230 values ≥1.8 ...
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bioRxiv - Microbiology 2021Quote: ... Total genomic DNA was extracted from each ked lysate and blood (camel, mouse, and rabbit) using DNeasy Blood & Tissue Kit (Qiagen, Hilden, Germany) following the manufacturer’s instructions.
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bioRxiv - Cell Biology 2023Quote: ... Genomic DNA was isolated from the tail tip of a wild-type C57BL/6J mouse using the DNEasy Blood and Tissue Kit (Qiagen, Hilden, Germany). The genomic C-terminal region of OGT was amplified using the Q5 Hot Start High Fidelity 2x master mix (New England Biolabs) ...
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bioRxiv - Cancer Biology 2023Quote: Total RNAs from mouse tumors were extracted and purified using the RNeasy Mini Kit and RNase-free DNase Set (QIAGEN, Valencia, CA) following the protocol provided by the manufacturer ...
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bioRxiv - Biochemistry 2023Quote: Mouse liver RNA was extracted from 10 mg (± 2 mg) of preserved tissue using RNEasy kits and QIAshredders (QIAGEN; Germantown, MD, USA). Aliquots of RNA from each sample were assessed on an Agilent 2100 Bioanalyzer (Agilent Technologies ...
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bioRxiv - Molecular Biology 2024Quote: Total RNA was isolated from blood-free cranial lobes of the right mouse lung using the RNeasy Tissue Mini Kit (Qiagen, Hombrechtikon, Switzerland). Isolated RNA was reverse-transcribed into cDNA using the Transcriptor First Strand cDNA Synthesis Kit (Roche Diagnostics ...
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bioRxiv - Microbiology 2024Quote: ... Parasite genomic DNA was purified from the blood of a transfer mouse using the Qiagen QIAamp DNA Blood Kit (Qiagen, Cat# 51106), and genotyping PCR was performed to assess integration into the target locus ...