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Citations for Qiagen :
1751 - 1800 of 3717 citations for 4 Methoxy 2 2 3 4 5 5 Hexacb 13C12 99% 50 Ug Ml In Toluene since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2019Quote: Genomic DNA was isolated from 3T3-L1 preadipocytes (day 0) and differentiated adipocytes (day 5) using DNeasy Tissue kit (Qiagen). Two µg of each DNA sample was bisulfite modified using EpiTect Bisulfite kit (Qiagen) ...
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bioRxiv - Neuroscience 2019Quote: ... RNA clean-up from TRAP samples and 5% of the unbound fractions from TRAP samples was performed using RNeasy Mini Kit (Qiagen) following manufacturer’s instructions ...
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bioRxiv - Microbiology 2019Quote: ... Approximately 5×106 HEK293 cells were used to isolate RNA with the All Prep RNA/DNA Mini Kit (Qiagen; 80204). cDNA was generated using 1μg of RNA with oligo(dT ...
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bioRxiv - Microbiology 2020Quote: ... Cells were lysed by boiling (5 min 95°C) followed by bead beating (3mm beads, 30 Hz for 1 min) (TissueLyser II, Qiagen) and sonication bath (3×10 sec at 4°C ...
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bioRxiv - Immunology 2020Quote: ... Within these gates CD8+MR1-5-OP-RU- and CD8+MR1-5-OP-RU+ T cell populations were sorted directly into lysis buffer for RNA isolation (RNeasy Plus Micro kit, Qiagen). Purity of ungated sorted CD8+humMR1-5-OP-RU+ cells was between 91-99% (Fig ...
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bioRxiv - Plant Biology 2021Quote: ... Approximately 250 plants showing the long root phenotype of the revertant were selected at 5 DAS and pooled for DNA extraction using the DNeasy Plant Mini Kit (Qiagen) according to the manufacturer’s instructions ...
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bioRxiv - Evolutionary Biology 2020Quote: ... several loci were amplified simultaneously from 1 μl of extracted DNA using 5 μl of the Multiplex PCR Master Mix (Qiagen) and varying amounts of the pooled primer mixes (Pool A ...
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bioRxiv - Microbiology 2020Quote: ... 5 µl RNA was used in a one-step real-time RT-PCR using the Rotor-Gene probe kit (Qiagen) according to instructions of the manufacturer ...
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bioRxiv - Microbiology 2021Quote: ... samples were weighed and homogenized in DMEM containing 10% FBS and 1% antibiotics using 5 mm stainless steel beads (Qiagen) and the TissueLyser II (Qiagen) ...
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bioRxiv - Pathology 2021Quote: ... 24 hours after HCort treatment all medium was removed using the Bluewasher (BlueCatBio) and cells were lysed for 5 minutes using RLT plus buffer (Qiagen), snap frozen on dry-ice and stored at −80 °C ...
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bioRxiv - Plant Biology 2019Quote: Total RNA was isolated from 5 DAC shoot cuttings of WT and miR476a-OE plants using RNeasy Plant Mini Kit (QIAGEN). Messenger RNA was purified and used for Illumina sequencing according to the guide of the Illumina mRNA sequencing sample preparation ...
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bioRxiv - Pathology 2021Quote: Striped dolphin brain and lung samples were submitted to total RNA extraction using the TissueLyser II (QI-AGEN) and highspeed shaking in Eppendorf tubes with stainless steel beads (5 mm diameter, QIAGEN). Homogenates were centrifuged at 14,000 rpm for 3 min to remove tissue debris ...
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bioRxiv - Evolutionary Biology 2021Quote: ... Genomic DNA was extracted from a pooled sample of 5 individuals using a DNeasy Blood & Tissue kit (Qiagen, Hilden, Germany). Extracted DNA was randomly fragmented to a size of 350bp then end-polished ...
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bioRxiv - Neuroscience 2021Quote: ... The frozen brain tissue blocks of WRM and BF were boiled for 8 min and homogenized in 5% acetic acid using a TissueLyser LT (Qiagen) for 6 min at 50 Hz ...
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bioRxiv - Microbiology 2021Quote: ... total environmental DNA was extracted from approximately 5 g of sediment subsampled from inner part of WRC using DNeasy PowerMax Soil Kit (Qiagen) with a minor modification 79 ...
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bioRxiv - Microbiology 2022Quote: ... Genomic DNA was extracted from a pooled sample of 5 individuals using a DNeasy Blood & Tissue kit (Qiagen, Hilden, Germany). Extracted DNA was randomly fragmented to a size of 350bp then end-polished ...
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bioRxiv - Developmental Biology 2021Quote: Total RNA was collected from multiple stages of zebrafish development (30%-epiboly, 9 somite, Prim-5, High-pec and Larvae) using the miRNeasy kit (Qiagen), according to the manufacturer’s instructions ...
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bioRxiv - Biophysics 2021Quote: ... Capture probes that contained locked nucleic acid (LNA) residues were purchased either from IDT with a 5′ Cy3 modification and HPLC purification or from Qiagen with a 5′ amino modification with HPLC purification ...
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bioRxiv - Neuroscience 2020Quote: ... PCR amplicons of exon 5 of the mouse Nprl3 gene were purified using a PCR purifications Kit (Qiagen, Hilden, Germany) and sent to the Massachusetts General Hospital Center for Computational and Integrative Biology DNA Core for whole amplicon next-generation sequencing (NGS ...
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bioRxiv - Microbiology 2021Quote: Total RNA (5×106 cells) was extracted from FEA or CRFK cell lines by using the RNeasy Plus Mini Kit (Qiagen). RNA from FEA ...
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bioRxiv - Molecular Biology 2020Quote: Total RNA for PIWIL3 5′ RACE was extracted from the ovaries of 8-week-old golden hamsters using ISOGEN (Nippon Gene) and RNeasy (Qiagen). 5′ RACE was performed using the SMARTer RACE 5/3 kit (Takara Bio ...
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bioRxiv - Microbiology 2022Quote: ... The eluted RNA from tissues and swabs was analyzed by one-step qRT-PCR using 5 μL input RNA with the QuantiFast Probe PCR kit (Qiagen) and primer/probe sets targeting the NiV Malaysia and HeV N gene ...
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bioRxiv - Developmental Biology 2022Quote: ... medial and proximal samples for each replicate were homogenised with an OMNI TH handheld 5 mm tip probe in 400 μl lysis buffer (Qiagen) for 20 seconds ...
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bioRxiv - Microbiology 2022Quote: ... the slurry was diluted to an OD600 of 5 and genomic DNA was purified using the DNeasy Blood & Tissue DNA purification kit (QIAGEN). In parallel ...
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bioRxiv - Immunology 2022Quote: Cells were centrifuged for 5 minutes at maximum speed and RNA was extracted with the RNeasy Plus Micro Kit (Qiagen) following the manufacturer’s recommendations ...
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bioRxiv - Microbiology 2022Quote: Cyanobacterial cells were collected by centrifugation of liquid cultures at 10000 g for 5 min and DNA was extracted from cell pellets with the DNeasy PowerBiofilm kit (Qiagen) following manufacturer’s instructions.
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bioRxiv - Immunology 2022Quote: ... and homogenized twice for 1-5 minutes each at 25Hz using a TissueLyser LT sample disruptor (Qiagen, Part No. 85600) with a 12 tube Tissue Lyser LT adapter (Qiagen ...
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bioRxiv - Molecular Biology 2022Quote: ... the nuclear pellet was sonicated for 5 min and genomic DNA was extracted using the QIAamp DNA Mini kit (Qiagen) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2022Quote: ... the nuclear pellet was sonicated for 5 min and nuclear gDNA was extracted following the protocol of the DNeasy Blood & Tissue kit (Qiagen) with addition of RNase A treatment.
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bioRxiv - Cell Biology 2022Quote: ... For each experimental condition total RNA was prepared from 5 OD600 units of cells using the RNeasy Plus RNA Isolation Kit (Qiagen). The synthesis of cDNA was performed using 500 ng of prepared RNA ...
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bioRxiv - Cancer Biology 2022Quote: RNA was isolated from 5 million cells per cell line using the Qiagen AllPrep DNA/RNA extraction kits (Qiagen, 80004). Nucleic acid quantification and quality detection were performed and RNA sequencing libraries were generated via Illumina Stranded mRNA prep ...
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bioRxiv - Cancer Biology 2022Quote: Total RNA from 5-20 mg of melanoma primary and metastatic tissues was extracted using AllPrep DNA/RNA Mini isolation kit (Qiagen). A total of 100 ng of total RNA was used as input for RNA- seq libraries using SMARTer Stranded Total RNA-seq - Pico input (Takara Bio USA ...
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bioRxiv - Microbiology 2023Quote: ... These tissues were then weighed and homogenized by either Omni homogenizer (Omni International, Omni Tissue Homogenizer (TH) 115V) or by 5-mm stainless steel beads with a TissueLyser (Qiagen) and used for onward analysis of infectious virus and vRNA quantification ...
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bioRxiv - Microbiology 2023Quote: ... DNA for metagenomes was extracted from 5 and 0.2 μm filters (47 mm diameter) using the DNAeasy Power Soil Pro kit (Qiagen, Sweden). DNA from viral fraction (142 mm diameter membranes ...
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bioRxiv - Neuroscience 2023Quote: ... After this step the samples were adjusted to RT for 5 minutes and processed using the RNeasy Lipid Tissue Mini Kit (Qiagen) as described in the user manual ...
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bioRxiv - Cancer Biology 2023Quote: ... RNA extraction from all fractions was performed using Qiazol at 65°C for 5 minutes with regular mixing and then purified with miRNeasy kit (Qiagen) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2023Quote: ... samples were incubated with 100 µL DTT (100 mM) at RT for 5 min and recovered by the RNeasy clean up kit (Qiagen) using the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2023Quote: ... The DNA was resolved by 1.5 % agarose followed by excision and isolation within the size range of 300-600 bp using Gel Extraction Kit (Qiagen). NGS libraries were pooled and quantified by qPCR using NEBNext Library Quant Kit (NEB) ...
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bioRxiv - Microbiology 2023Quote: ... Cells were washed and incubated in Proteinase K (Roth) and Ribonuclease A (Roth; 5 min, RT) before isolating cellular and viral DNA with DNeasy Blood & Tissue Kit (Qiagen). qPCR was performed using FastStart Universal Probe Master (Roche ...
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bioRxiv - Microbiology 2023Quote: ... Total RNA was isolated from pooled triplicates 5 hpi with RNeasy Mini columns according to the manufacturers instructions (RNeasy Kits, Qiagen) and strand-specific qPCRs were performed as described previously (38) ...
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bioRxiv - Cancer Biology 2023Quote: ... The material stored in Qiazol was heated at 65°C for 5 min and RNA was extracted using the RNeasy Lipid Tissue Kit including the optional DNase digestion (Qiagen). RNA quality and concentration were assessed using a 2100 Bioanalyzer (Agilent Technologies ...
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bioRxiv - Genomics 2022Quote: ... We incubated the reaction for 5 minutes at 55 °C in a G-Storm GS1 (G-Storm) thermal cycler and after incubation added 5 μl Buffer PB (19066, QIAGEN) to inactivate and strip the transposase from the tagmented DNA ...
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bioRxiv - Immunology 2023Quote: ... supplemented with 10% FBS and 1x antibiotic-antimycotic by a TissueLyser II bead mill with 5-mm stainless steel beads (Qiagen). After brief centrifugation ...
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bioRxiv - Microbiology 2023Quote: ... calf muscle and pancreas were collected in 500 μl of PBS containing one (heart and pancreas) or two (calf muscle) 5 mm stainless steel beads (QIAGEN), homogenized with TissueLyser II (QIAGEN ...
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bioRxiv - Microbiology 2023Quote: ... The sample was stored with RNAprotect following the manufacturer protocol (Protocol 5 from RNAprotect Bacteria Reagent Handbook 01/2020, Qiagen) at -80°C until processed ...
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bioRxiv - Immunology 2023Quote: ... 5×104 cells were resuspended in buffer RLT supplemented with ý-mercaptoethanol and processed with an RNeasy Micro Kit (Qiagen) per manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2023Quote: ... by centrifugation (200 x g for 5 min at 25°C. Whole RNA was isolated using the Qiagen RNeasy kit (Qiagen) according to the manufacturer’s description ...
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bioRxiv - Molecular Biology 2023Quote: ... treated with either the Pin-point system or SpCas9 was extracted 5 days post electroporation using the Gentra Puregene Cell Kit (Qiagen) following manufacturer’s instructions ...
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bioRxiv - Genomics 2023Quote: ... The bead-bound gDNA was isothermally amplified for 3 hours at 30 °C then 10 minutes at 65 °C using a miniaturised (1/5 vols) Repli-g Single-Cell assay (Qiagen). The amplified gDNA was cleaned up with 0.8 × vols Ampure XP and 80 % ethanol ...
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bioRxiv - Cancer Biology 2023Quote: ... fresh 5 mM DTT) and incubated for 45 minutes at 65°C before addition of 40 μg Proteinase K (Qiagen) and further incubation for 1.5 hours at 37°C ...