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Citations for Qiagen :
2001 - 2050 of 3717 citations for 4 Methoxy 2 2 3 4 5 5 Hexacb 13C12 99% 50 Ug Ml In Toluene since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genetics 2022Quote: ... and extracted and amplified from vitrified blastocysts by thawing in phosphate-buffered saline supplemented with 5% bovine serum albumin and processing with REPLI-g whole genome amplification methods (Qiagen, Cat. #150343). Informed consent was obtained from all participants (custodians of the blastocysts ...
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bioRxiv - Developmental Biology 2022Quote: ... cDNA synthesis of 100 ng total RNA was performed with miRCURY LNA RT Kit (10 µl volume reaction) which adds a 5’ universal tag of a poly(A) tail to mature miRNA templates (QIAGEN, Germantown, MD). cDNA template was diluted 1:10 ...
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bioRxiv - Molecular Biology 2020Quote: ... Cells were pelleted at 500 g for 5 min and total RNA was extracted using RNeasy Mini Kit (QIAgen, Cat No. 74104), including DNAse digestion step (QIAgen ...
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bioRxiv - Neuroscience 2021Quote: ... tMCAO mice at 24 h and 48 h post reperfusion were used for total mRNA isolation according to the protocols of the Isol-RNA Lysis Reagent (5 PRIME, Gaithersburg, USA) and the bead-milling TissueLyser system (Qiagen, Hilden, Germany). QuantiTect® Reverse Transcription Kit (Qiagen ...
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bioRxiv - Immunology 2020Quote: YFP+ Treg cells were sorted from spleen and LN of WT or Usp22fl/flFoxp3YFP-Cre mice (n=5 per group) and total RNA was isolated from 1×106 cells per sample using an RNeasy Mini Kit (Qiagen, Cat# 74104) as previously described47 ...
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bioRxiv - Cancer Biology 2019Quote: ... The long genomic DNA was isolated from 5-10mg tumor core using Gentra Puregene Tissue Kit following the manufacturer’s instructions (Qiagen, Cat. No 158667). Briefly ...
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bioRxiv - Developmental Biology 2022Quote: ... The PCR strips were then incubated for 15 min at 37 °C in a thermocycler and the reaction was stopped by adding 5 μl RLT plus buffer (Qiagen, Cat# 1053393) to each well ...
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bioRxiv - Molecular Biology 2022Quote: ... and isolated keratinocytes and fibroblasts from the skin and Wound7 (n = 5/each group) (Table 1 and Table S1) by using the miRNeasy Mini kit (Qiagen, Hilden, Germany) and prepared for library construction ...
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bioRxiv - Genomics 2022Quote: ... The mixture was centrifuged at 13,000 rpm for 5 mins and 140 µl of supernatant used for RNA extraction (Qiagen viral RNA kit). The final TNA was eluted in 60 µl of molecular grade water and stored at −80°C.
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bioRxiv - Biochemistry 2023Quote: ... gDNA was isolated from 5 x 106 Colo205 cells using the Blood and Cell Culture DNA Maxi Kit (Qiagen, catalog no. 13362) according to the manufacturer’s instructions ...
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bioRxiv - Plant Biology 2023Quote: ... unexpanded leaves was extracted from 14 individuals (7 early-blooming, 5 late-blooming, and the two parents) with a DNeasy Mini Plant Kit (Qiagen, Hilden, Germany) and quantified with a Quant-iTTM PicoGreenTM dsDNA assay kit (Thermo Fisher Scientific ...
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bioRxiv - Plant Biology 2023Quote: ... and pXVE::YFP-cPMEI8 plants treated with control MS and 5 µM estradiol for 24 h using the RNeasy Plant Kit (QIAGEN, Hilden, Germany). For RNA isolation from meristematic and elongation zones ...
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bioRxiv - Immunology 2023Quote: ... Tissue RNA was isolated from preserved samples using 30 minutes of automated homogenization at 30 Hz with 5 mm ball-bearings in RLT Plus Lysis Buffer (Qiagen TissueLyser II), RNA was then isolated using a RNeasy Plus Micro Kit (Qiagen ...
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bioRxiv - Molecular Biology 2023Quote: Parotid glands were removed from mice (n=5/group) and total DNA was immediately isolated using the DNeasy Blood & Tissue Kit (Ref. No. 69504, Qiagen, Hilden, Germany). DNA samples were diluted with nuclease-free water to a concentration of 10 ng/uL ...
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bioRxiv - Immunology 2024Quote: Genomic DNA was then isolated from 1-5 million isolated PBMCs using the DNeasy Blood and Tissue kit (Qiagen; Cat. No. 69504) following manufacturer instructions ...
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bioRxiv - Cancer Biology 2023Quote: ... 3’mRNA-seq libraries were prepared using QIAseq UPX 3’ Transcriptome Kit (QIAGEN). In brief ...
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bioRxiv - Evolutionary Biology 2020Quote: ... Approximately half the volume of each feces sample (100-200 mg) was immersed in 3 ml of Inhibit EX Buffer (QIAGEN, Hilden) and stored at room temperature ...
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bioRxiv - Immunology 2022Quote: ... The lysate was then clarified by centrifugation at 16,000 x g for 45 minutes and then applied to 3 mL Ni-NTA resin (Qiagen, Valencia, CA) equilibrated with buffer A ...
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bioRxiv - Cancer Biology 2023Quote: ... medium was removed and cells were lysed on the plate by addition of 3 ml of lysis reagent RLT Plus (Lot 169010027, Qiagen, Germany). 1 ml of lysate was transferred to a 2 ml Eppendorf tube ...
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bioRxiv - Microbiology 2020Quote: Total RNA extraction was performed using a squash buffer (10 mM Tris base, 1 mM EDTA, 50 mM NaCl) supplemented with 1:8 part Proteinase K (Qiagen, 15mg/ml). Mosquito abdomens with the midguts and heads with thoraces were individually collected in 50 μl of squash buffer at 7 and 14 days ...
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bioRxiv - Microbiology 2023Quote: ... Single colonies were picked and grown overnight in LB broth with 50 μg/mL Kanamycin before plasmids were isolated using a QIAprep Spin Mini Kit (Qiagen, Germantown, MD), confirmed by restriction enzyme digest ...
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bioRxiv - Biophysics 2021Quote: ... and the bindingPCA plasmid containing the ligand GAB2 or CRIPT fused to DHFR1,2 (pGJJ006 and pGJJ066 respectively) were digested with the same enzymes and purified using the QIAquick Gel Extraction Kit (QIAGEN).
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bioRxiv - Cell Biology 2020Quote: ... RNA was further digested with TURBO DNase overnight at 37°C (10 U per 2 μg of RNA) and purified with the RNeasy MinElute Cleanup Kit (Qiagen) following the manufacturer’s instructions.
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bioRxiv - Cell Biology 2020Quote: ... TRAF3 KO1 and TRAF3/p100 double KO cells from 2 experimental repeats was isolated using the RNeasy Mini Kit (Qiagen). RNA quality control was performed using a 2100 Bioanalyzer (Agilent Technologies) ...
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bioRxiv - Developmental Biology 2021Quote: ... run on a 2% agarose gel and a band of 300bp approximately was cut and gel purified using QIAquick Gel Extraction Kit (QIAGEN). Library concentration was determined with KAPA Library Quantification Kit for Illumina Platforms (Kapa Biosystems) ...
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bioRxiv - Immunology 2022Quote: ... the DNA was extracted from 100 mg mouse fecal samples (9-week-old, 2 weeks after final oral gavage treatment) using QIAamp PowerFecal Pro DNA kit (Qiagen) following the manufacturer’s instruction ...
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bioRxiv - Biophysics 2022Quote: ... Purified protein was diluted in PBS buffer (pH = 7.2) and the fluorescence intensity was recorded at 60 °C in the Rotor-Gene 6600 real-time PCR cycler (Qiagen) for 18 h ...
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bioRxiv - Genomics 2020Quote: ... total RNA was extracted from cells grown in five different salinity treatments (0, 2, 12, 24, 36 parts per thousand) using the RNeasy Plant Kit (Qiagen), and 15 Illumina libraries were prepared using the Kapa mRNA HyperPrep kit and sequenced on the Illumina HiSeq2000 platform at the Beijing Genomics Institute.
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bioRxiv - Genomics 2019Quote: Total RNAs from the knockdown (shCTCF#1 and shCTCF#2) and control (shLuc) cells were extracted using miRNeasy Micro Kit (QIAGEN). cDNA was synthesized by using oligo (dT)20 and SuperScript III Reverse Transcriptase (Invitrogen ...
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bioRxiv - Immunology 2021Quote: ... Twenty consecutive 10 μm sections were placed on glass slides for subsequent IHC (sections 1-2, 7-8, 13-14, & 19-20) or placed in 600 μl buffer RLT (Qiagen) containing 1% 2-mercaptoethanol (Sigma-Aldrich ...
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bioRxiv - Genomics 2019Quote: ... Purified products were loaded onto a 2% E-gel and gel extracted using a QiaQuick Gel Extraction kit (Qiagen 28704). The molarity of the gel-extracted PCR product was quantified using KAPA library quant (KK4824 ...
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bioRxiv - Genomics 2020Quote: The commonly up- or downregulated genes in cluster 1 and 2 compared to cluster 0 of untreated MDA-MB-231 cells were subjected to the Ingenuity Pathway Analysis (IPA) (QIAGEN) (Kramer ...
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bioRxiv - Cancer Biology 2019Quote: Mouse muscles was pulverized in liquid nitrogen and homogenized 2 × 0.5 minutes at 30 Hz using a TissueLyser II bead mill (Qiagen, USA) in ice-cold homogenization buffer ...
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bioRxiv - Genetics 2019Quote: ... purified using an E-gel electrophoresis system (Agarose 2%) followed by column purification with a MinElute PCR Purification Kit (Qiagen). In order to introduce the doped sequence in the full-length TDP-43 sequence the purified oligonucleotide was cloned into 100 ug of linearized pRS416 Gal TDP-43 by a Gibson approach (Supplementary Table 1 ...
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bioRxiv - Microbiology 2019Quote: ... medium at 37°C and was made competent with rubidium chloride according to the method provided in the QIAexpressionist manual protocol 2 (Qiagen). When antibiotic selection was required ...
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bioRxiv - Immunology 2020Quote: RNA was extracted from IEC isolations by adding a volume containing 2 x 106 cells in PBS to QIAshredder spin columns (QIAGEN) followed by centrifugation at 9,300 g for 1 min ...
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bioRxiv - Evolutionary Biology 2019Quote: ... 2 to 10 g slices of liver tissue were homogenized in cell lysis solution and proteinase K (Qiagen, Germantown, USA). DNA was extracted from the liver homogenates and resuspended in buffer ...
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bioRxiv - Biochemistry 2019Quote: Genomic DNA was extracted from 2 g of frozen Annona muricata seed powder with the DNEasy Mericon Food Kit (QIAGEN) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2019Quote: ... Alterations to the protocol included additional lysozyme treatment and bead beating with stainless steel beads for 2 min/20 Hz in a tissue lyser (Qiagen). In each sample including the blank control ...
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bioRxiv - Genomics 2020Quote: ... SARS-CoV-2 nucleic acids were isolated from 300 µl viral transport media using the QIAamp Viral RNA Mini kit (Qiagen) according to the manufacturer’s protocol ...
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bioRxiv - Genetics 2020Quote: ... Total viral RNA was extracted from patient specimens and tested for the presence of SARS-CoV-2 with an EZ1 virus extraction kit and EZ1 Advanced XL instrument with the Virus card (Qiagen), or QIASymphony DSP Virus kit and QIASymphony instrument (Qiagen).
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bioRxiv - Immunology 2021Quote: ... Copies of SARS-CoV-2 nucleocapsid (N) gene in homogenized tissues were determined using QuantiNova SYBR Green PCR kit (Qiagen) along with 2019-nCoV RUO Kit (Integrated DNA Technologies ...
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bioRxiv - Microbiology 2021Quote: The cells were lysed by high speed vortexing for 2 minutes using lysing matrix E tubes (MP Biomedical) in buffer RLT (Qiagen) amended with 1% 2-mercaptoethanol ...
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bioRxiv - Cell Biology 2021Quote: ... Mitochondria were purified from approximately 2*106 cultured HeLa cells continuously expressing the mitochondrial matrix marker su9-mCherry using the Qproteome Mitochondria Isolation Kit (Qiagen) following manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2020Quote: ... The elution supernatants were combined (a 20 μL elute sample was taken and combined with 20 μL 2 × SDS loading buffer) and RNAs were extracted using a miRNeasy kit (QIAGEN) according to the manufacturer’s instructions.
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bioRxiv - Bioengineering 2020Quote: ... Nested PCR was then performed with 2 μl of cDNA in 25 μl reactions with the HotStar Taq Plus Kit (QIAGEN), using 5’ leader sequence–specific and 3’ IgG-specific primers (64) ...
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bioRxiv - Bioengineering 2021Quote: The changes in mRNA levels were compared by Relative Expression Software Tool (REST® 2009 v2.013) using 2-ΔΔCT relative quantitative analysis and also confirmed by GeneGlobe Data Analysis Center (Qiagen). Student t test has been used throughout the study ...
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bioRxiv - Bioengineering 2021Quote: ... dsDNA product was run on a 2% agarose gel and extracted with the Qiaex II Gel Extraction Kit (Qiagen, 20021). dsDNA was transcribed to RNA using 5 reactions of the Durascribe transcription kit as described above ...
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bioRxiv - Bioengineering 2020Quote: ... Cells were harvested 2 days post-transfection and genomic DNA (gDNA) was purified with the QIAamp DNA Blood Mini Kit (Qiagen). The genomic region surrounding the pegRNA target site was then amplified by PCR with the following primers ...
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bioRxiv - Bioengineering 2020Quote: ... The PCR fragments were incubated with DpnI for 2 h at 37°C to degrade the template plasmids before being purified using a PCR purification kit (Qiagen). The purified PCR fragments were digested overnight at 37°C using NheI and XhoI and ligated overnight at 16°C to backbone vector pYZ125 ...