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Citations for Qiagen :
1701 - 1750 of 2200 citations for tert Butyl 3 5 2 amino ethyl thiophen 3 yl propionate since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2021Quote: Cells were seeded in 6-well and 12-well plates at a density of 1.25×105-5×105 cells per well and transfected with miR-320a mimic (Qiagen, 339173 YM00471432) or antagomir (GenePharma ...
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bioRxiv - Immunology 2021Quote: ... Single cells were sorted into individual wells of a 96-well plate containing 5 μl of lysis buffer (TCL buffer (Qiagen #1031576) with 1% of 2-b-mercaptoethanol) ...
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bioRxiv - Bioengineering 2021Quote: ... The 319 bp DNA size of PCR products were clarified by 1% agarose gel electrophoresis using 5 μL PCR products and remained DNA were purified by QIAquick PCR Purification Kit (Qiagen, 28104). The purified PCR products were sequenced by Sanger Sequencing approach (GeneWiz ...
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bioRxiv - Cancer Biology 2020Quote: ... cells were collected by centrifugation (5 min at 500 x g) and RNA was isolated using RNeasy Mini Kit (RNeasy Mini Kit, Qiagen, Germany) according to the manufacturer’s protocol ...
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bioRxiv - Neuroscience 2022Quote: ... and the tissue was homogenized with a 5 mm tungsten carbide bead in a bead mill homogenizer (Qiagen, Tissue Lyser II) at 30 Hz for 1.5 minutes ...
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bioRxiv - Molecular Biology 2022Quote: ... and the sample was divided into 5 parts and each part was purified using one PCR purification columns (PCR purification kit, Qiagen 28106). 50ul elution buffer was used the elute each column ...
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bioRxiv - Developmental Biology 2020Quote: ... Cells were centrifuged for 5 minutes at 300g and resuspended in 1mL PBS + RNase Inhibitors (Invitrogen AM2694, Qiagen Beverly Inc Y9240L) and fixed as previously described for split-pool ligation-based RNAseq ...
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bioRxiv - Biochemistry 2020Quote: ... embryo pellets were homogenized by cryo-milling after addition of a 2.5 or 5 mm steel bead using a tissue lyser (QIAgen TissueLyser II) at 25 Hz for 15 seconds 3-6 times ...
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bioRxiv - Genetics 2022Quote: ... 0.08M KOH was added to each well and incubated at room temperature (RT) for 5 min and then washed with EB (Qiagen, 19086). Next ...
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bioRxiv - Genomics 2022Quote: ... The swabs were placed in Red 1.5 mL RINO® screw-cap tubes (NextAdvance) pre-filled with RNase-free zirconium oxide beads and QIAzol Lysis Reagent (Qiagen) was added ...
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bioRxiv - Microbiology 2021Quote: ... 5) 240 µL of the supernatant is subjected to viral RNA extraction using the QIAamp Viral RNA Mini Kit (Qiagen, #52904), according to manufacturer’s instructions ...
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bioRxiv - Genetics 2020Quote: ... suspended in 1 mL of (v/v) 50% ACN and homogenized in a bead mill (50 Hz, 5 min; TissueLyser LT, Qiagen, Germany) using two 5 mm tungsten balls ...
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bioRxiv - Genomics 2021Quote: ... We checked ASOs penetration in cells by means of the 5′-FAM-labeled control ASO A provided by Qiagen (Figure S5f).
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bioRxiv - Neuroscience 2021Quote: ... 5 ng of each PCR product was pooled into one tube for purification using the QIAquick PCR purification kit (Qiagen #28106) and eluted into 30 µL Nuclease Free Water ...
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bioRxiv - Cancer Biology 2020Quote: ... 5 SCID mT3-2D subcutaneous tumors and the mT3-2D cell line using a DNeasy Blood and Tissue Kit (Qiagen, #69504). Indexed ...
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bioRxiv - Immunology 2021Quote: ... RNA was extracted from the colon tissue of mice (n=5) by using the RNeasy mini kit (Cat# 74104, Qiagen, Germany) following the manufacturer’s protocol ...
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bioRxiv - Immunology 2020Quote: ... Single CD3-CD8-CD14- CD16- CD20+CD38+ BG505 SOSIP+YU2-gp140+ B cells were sorted into individual wells of a 96-well plates containing 5 μl of a lysis buffer (Qiagen, 1031576) per well using a FACS Aria III (Becton Dickinson) ...
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bioRxiv - Developmental Biology 2020Quote: ... the beads were transferred to a new 0.5ml tube and incubated in 100 μL ChIP elution buffer (10mM Tris-HCl pH8.0, 5mM EDTA, 300mM NaCl, 0.5% SDS) containing 5 µL proteinase K (Qiagen, 20mg/ml stock) at 55°C for 2 h and then at 65°C for 4 h ...
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bioRxiv - Developmental Biology 2022Quote: ... The resulting 1.5 L bacterial culture was incubated at 37°C for 2h and a Maxiprep performed using Qiagen MaxiPlus kit (Qiagen #12963) to a resulting 1 mg of plasmid DNA ...
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bioRxiv - Genomics 2022Quote: ... Individual nuclei were sorted on a Sony Sorter SH800 with a 100 μm sorting chip into wells of a 96-well plate containing 5 μl of Buffer TCL (Qiagen #1031576) with 1% β-mercaptoethanol (ThermoFisher #21985023).
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bioRxiv - Microbiology 2022Quote: ... The cells were then centrifuged at 300g for 5 minutes and lysed using buffer RLT provided in the RNAeasy Plus Micro Kit (Qiagen; 74034). After the lysate was homogenized using the QIAshredder Kit (Qiagen ...
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bioRxiv - Microbiology 2023Quote: ... 5% CO2 for 72 hours before lysis and RNA extraction using the RNeasy Micro Plus extraction kit (Qiagen, Cat. No. 74034). A further technical replicate was resuspended in RPMI supplemented with 20% FCS ...
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bioRxiv - Molecular Biology 2023Quote: ... 500 ng of plasmid DNA was transfected into each well using Effectene (4 μL of enhancer and 5 μL of Effectene reagent; Qiagen 301427). Unless otherwise noted ...
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bioRxiv - Systems Biology 2024Quote: ... The samples were then cooled for 5 minutes on ice and transferred to a pre-spun 15 mL MaXtract tube (Qiagen 129065) containing an equal volume of chloroform to the acid phenol used ...
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bioRxiv - Microbiology 2024Quote: ... and 10 zirconium beads were added to approximately 5 mg of intestinal tissue stored at -80 °C in RNA later tissue reagent (Qiagen, Germany). A Tissue lyser (Qiagen ...
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Human CCR6+ Th cells show both an extended stable gradient of Th17 activity and imprinted plasticitybioRxiv - Immunology 2023Quote: ... for 3 h at 37°C under 5% CO2 and total cellular RNA was purified using PureLink™ RNA kit (Life Technology) or RNeasy Mini Kit (Qiagen) and the concentration and RNA Integrity Number (RIN ...
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bioRxiv - Immunology 2022Quote: ... following the final boost (Week 18 or 23) were sorted into individual wells of a 96-well plate containing 5 μL of lysis buffer (TCL buffer (Qiagen, 1031576) with 1% of 2-β-mercaptoethanol) ...
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bioRxiv - Cell Biology 2023Quote: ... Spheroids were transferred into PCR-clean 1.5 mL microtubes with 100 µL of lysis buffer (LB1 from Macherey-Nagel or 700 µL of Buffer RLT (79216, Qiagen). Lysates were snap-frozen and stored at −80 °C ...
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bioRxiv - Plant Biology 2023Quote: PCR primers 515/806 with barcoding on the forward primer were used in a 28 cycle PCR (5 cycle used on PCR products) using the HotStar Taq Plus Master Mix Kit (Qiagen, USA) to target the 16S rRNA gene for region V3 and V4 ...
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bioRxiv - Genomics 2023Quote: ... 50 nuclei were sorted into each well of 4 twin.tec™ 96 Well LoBind PCR Plates that had 5 uL of EB buffer (Qiagen, 19086), 0.5 uL of 5 x mRNA Second Strand Synthesis buffer (New England Biolabs ...
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bioRxiv - Microbiology 2022Quote: ... The spooled DNA was washed with approximately 5 mL of 70% ethanol and dried before being suspended in 300µL of EB buffer (QIAGEN, Hilden, Germany). Purified DNA was quantified by Qubit (Thermofisher).
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bioRxiv - Cancer Biology 2023Quote: Ctnnb1 exon 3 skipping was confirmed in several tumour samples by extracting total RNA from 5-20 mg of tissue using the RNeasy Plus Universal minikit (Qiagen, #73404). cDNA was synthesized using the RevertAid protocol (ThermoScientific ...
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bioRxiv - Microbiology 2023Quote: ... 20-40 mg of tissue was placed in a tube containing 600 μl of OptiMEM and a 5 mm stainless steel bead (Qiagen; #69989) and homogenized in the Qiagen TissueLyser II by two dissociation cycles (1,800 oscillations/minute for 2 minutes ...
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bioRxiv - Synthetic Biology 2023Quote: ... 5 mL of the culture was immediately treated with a double volume (10 mL) of RNA protect bacteria reagent (Qiagen, Germany) for 5 minutes at room temperature ...
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bioRxiv - Genomics 2023Quote: The bacterial 16S rRNA V4 variable region was amplified for 5 cycles with the HotStarTaq Plus Mastermix Kit (Qiagen, Hilden, Germany). Samples were purified with Ampure XP beads (Beckman Coulter ...
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bioRxiv - Biochemistry 2023Quote: ... Cell lysates were clarified by additional centrifugation at 14,000 xg for 30 minutes and supernatants were incubated 1 hour at 4 °C with 5 mL of His-Pur™ Ni-NTA resin (Qiagen) previously washed 3 times with 25 mL of Lysis Buffer ...
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bioRxiv - Molecular Biology 2023Quote: Total DNA was extracted from mouse parotid gland tissue (n=5/group) following the manufacturer’s protocol for DNeasy Blood & Tissue Kit (Ref. No. 69504, Qiagen, Hilden, Germany). Mitochondrial DNA (mtDNA ...
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bioRxiv - Developmental Biology 2024Quote: ... Transfection was conducted with 5 μg plasmids (act-GAL4, UAS-lbm-GFP, UAS-upd2-mCherry) using Effectene Transfection Reagent (QIAGEN, #301425).
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bioRxiv - Cancer Biology 2024Quote: ... additional DNA from 1-5 mL of plasma was extracted using the QIAamp® Circulating Nucleic Acid Kit (Qiagen, catalogue # 55114). Illumina sequencing libraries were constructed using SRSLY® PicoPlus DNA NGS Library Preparation Kit (ClaretBio – Cat ...
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bioRxiv - Biochemistry 2024Quote: HEK293T cells were plated in 10 cm plates at 5 million cells/plate and the next day transiently transfected with the indicated plasmids using PolyFect (Qiagen 301105) according to the manufacturer’s instructions ...
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bioRxiv - Plant Biology 2024Quote: ... Colony PCR was performed with KOD One™ PCR Master Mix (Toyobo) to screen colonies before recovering plasmids with a 5 mL overnight culture and miniprep (QIAgen).
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bioRxiv - Evolutionary Biology 2024Quote: Total RNA was extracted from 5- or 7-day post-fertilization (dpf) stage animals using RNeasy Mini Kit (Qiagen, cat. 74104) according to the manufacturer’s instructions ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: Flash frozen LV tissue blocks from the infarct and border zones were blinded for identification and homogenised using a 5 mm stainless steel bead (Qiagen, 69989) and TissueLyserII (Qiagen ...
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bioRxiv - Genomics 2024Quote: ... a buffy coat from a fresh 5-ml blood sample tube was aliquoted for peripheral blood mononuclear cells (PBMCs) isolation using RBC Lysis Solution (QIAGEN, 158904). The purified PBMCs were counted ...
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bioRxiv - Immunology 2024Quote: ... as found in our previous study.5 Total RNA was extracted using the RNeasy Fibrous Tissue Mini Kit (Qiagen, Germantown, MD) following manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2024Quote: ... 5-day cell cultures were harvested and total genomic DNA was isolated using the DNeasy blood and tissue kit (QIAGEN 69506). This DNA was sequenced and analysed for mutations.
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bioRxiv - Cell Biology 2024Quote: RNA at the defined stages of differentiation (day 0, 1, 5, 8, 11, 14, 17, 27, 35, 50) was extracted using RNeasy Plus mini kit (Qiagen, 74136) according to the manufacturer’s guidelines ...
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bioRxiv - Evolutionary Biology 2024Quote: ... They were placed individually into 2 ml microcentrifuge tubes with 1 ml of distilled water and a stainless-steel bead (Ø 5 mm, and homogenised with a Qiagen TissueLyser LT at a frequency of 30 Hz for one minute ...
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bioRxiv - Biophysics 2021Quote: ... The solubilized fraction was then incubated for 2 h with 300 µL of pre-equilibrated Ni-NTA resin (QIAGEN) in Ni-NTA buffer (50 mM Tris-HCl ...
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bioRxiv - Biophysics 2021Quote: cfDNA was isolated from 2 mL of plasma for each sample via the QIAamp Circulating Nucleic Acid kit (Qiagen). ddPCR was performed using the QX200 Droplet Digital PCR System (Bio-Rad) ...