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Citations for Qiagen :
1601 - 1650 of 2200 citations for tert Butyl 3 5 2 amino ethyl thiophen 3 yl propionate since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2023Quote: DNA was extracted from the bacterial strains grown in VL55 medium with 5 mM pyruvate using the Qiagen Genomic-tip 100/G DNA isolation kit and associated DNA buffers (Qiagen) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2023Quote: ... Metagenomic sequencing libraries were constructed from extracted DNA samples with 10 μL (1/5 volume) reactions using the QIAseq FX DNA Library Kit (QIAGEN). Each metagenomic sequencing library was sequenced using the Illumina NextSeq 2000 System 2 x 150 bp configuration.
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bioRxiv - Microbiology 2023Quote: DNA was extracted from Day 11 populations (n=20, 5/treatment) using Qiagen DNeasy Blood and Tissue Kit (Qiagen, Germany) adapted from the manufacturer’s instructions to include a lysostaphin lysis step(50) ...
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bioRxiv - Cancer Biology 2023Quote: ... we FACS-sorted single nuclei in 384-well plates prefilled with 5 μL of Vapor-Lock (Qiagen, cat. no. 981611) per well ...
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bioRxiv - Cancer Biology 2023Quote: ... of 5 million AMO1 shIRE1 cells previously treated per condition was isolated using the AllPrep DNA/RNA Mini Kit (Qiagen). gDNA was quantified using Nanodrop spectrophotometer (Thermo Fisher Scientific ...
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bioRxiv - Cancer Biology 2023Quote: ... fresh 5 mM DTT) and incubated for 45 minutes at 65°C before addition of 40 μg Proteinase K (Qiagen) and further incubation for 1.5 hours at 37°C ...
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bioRxiv - Molecular Biology 2023Quote: cDNA was produced from 1 μg of total RNA (final cDNA concentration 5 ng/ml) using QuantiTech Reverse Transcription kit (Qiagen) according to the manufacturer’s instructions.
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bioRxiv - Microbiology 2022Quote: ... The eluted RNA from tissues and swabs was analyzed by one-step qRT-PCR using 5 μL input RNA with the QuantiFast Probe PCR kit (Qiagen) and primer/probe sets targeting the NiV Malaysia and HeV N gene ...
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bioRxiv - Developmental Biology 2022Quote: ... medial and proximal samples for each replicate were homogenised with an OMNI TH handheld 5 mm tip probe in 400 μl lysis buffer (Qiagen) for 20 seconds ...
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bioRxiv - Microbiology 2022Quote: ... the slurry was diluted to an OD600 of 5 and genomic DNA was purified using the DNeasy Blood & Tissue DNA purification kit (QIAGEN). In parallel ...
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bioRxiv - Immunology 2022Quote: Cells were centrifuged for 5 minutes at maximum speed and RNA was extracted with the RNeasy Plus Micro Kit (Qiagen) following the manufacturer’s recommendations ...
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bioRxiv - Microbiology 2022Quote: Cyanobacterial cells were collected by centrifugation of liquid cultures at 10000 g for 5 min and DNA was extracted from cell pellets with the DNeasy PowerBiofilm kit (Qiagen) following manufacturer’s instructions.
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bioRxiv - Immunology 2022Quote: mRNA was isolated from Boyden chamber migration assay-derived Treg pellets from 5 HD and 4 uRRMS patients using RNeasy micro kit (Qiagen) according to manufacturer’s instructions ...
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bioRxiv - Immunology 2022Quote: ... and homogenized twice for 1-5 minutes each at 25Hz using a TissueLyser LT sample disruptor (Qiagen, Part No. 85600) with a 12 tube Tissue Lyser LT adapter (Qiagen ...
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bioRxiv - Microbiology 2023Quote: ... Total RNA was isolated from pooled triplicates 5 hpi with RNeasy Mini columns according to the manufacturers instructions (RNeasy Kits, Qiagen) and strand-specific qPCRs were performed as described previously (38) ...
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bioRxiv - Cancer Biology 2023Quote: ... The material stored in Qiazol was heated at 65°C for 5 min and RNA was extracted using the RNeasy Lipid Tissue Kit including the optional DNase digestion (Qiagen). RNA quality and concentration were assessed using a 2100 Bioanalyzer (Agilent Technologies ...
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bioRxiv - Biophysics 2023Quote: ... The lysate was pelleted by centrifugation at 30,000 x g for 30 min and the supernatant was mixed with 5 mL pre-equilibrated Ni-NTA resin (Qiagen) in a 50 mL falcon tube ...
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bioRxiv - Genomics 2022Quote: ... We incubated the reaction for 5 minutes at 55 °C in a G-Storm GS1 (G-Storm) thermal cycler and after incubation added 5 μl Buffer PB (19066, QIAGEN) to inactivate and strip the transposase from the tagmented DNA ...
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bioRxiv - Immunology 2023Quote: ... supplemented with 10% FBS and 1x antibiotic-antimycotic by a TissueLyser II bead mill with 5-mm stainless steel beads (Qiagen). After brief centrifugation ...
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bioRxiv - Microbiology 2023Quote: ... calf muscle and pancreas were collected in 500 μl of PBS containing one (heart and pancreas) or two (calf muscle) 5 mm stainless steel beads (QIAGEN), homogenized with TissueLyser II (QIAGEN ...
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bioRxiv - Microbiology 2023Quote: ... The sample was stored with RNAprotect following the manufacturer protocol (Protocol 5 from RNAprotect Bacteria Reagent Handbook 01/2020, Qiagen) at -80°C until processed ...
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bioRxiv - Biophysics 2023Quote: ... Cleaved PCNA was separated from His-SUMO and uncleaved His-SUMO-PCNA by incubating the protein solution with 5 ml Ni-NTA suspension (Qiagen) resuspended in solution D on a tilting table for 30 min ...
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bioRxiv - Cell Biology 2023Quote: ... and added to appropriate volumes of buffer RLT and homogenised at 50 Hz for 5 minutes using the TissueLyser LT (Qiagen), until all tissue was homogenised ...
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bioRxiv - Immunology 2023Quote: ... 5×104 cells were resuspended in buffer RLT supplemented with ý-mercaptoethanol and processed with an RNeasy Micro Kit (Qiagen) per manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2023Quote: ... by centrifugation (200 x g for 5 min at 25°C. Whole RNA was isolated using the Qiagen RNeasy kit (Qiagen) according to the manufacturer’s description ...
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bioRxiv - Molecular Biology 2023Quote: ... treated with either the Pin-point system or SpCas9 was extracted 5 days post electroporation using the Gentra Puregene Cell Kit (Qiagen) following manufacturer’s instructions ...
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bioRxiv - Immunology 2024Quote: RNA was isolated from 5×106 sorted WSU-FSCCL IgG+ and IgM+ cells using the RNeasy mini kit (Qiagen, 74004). RNA quality control was performed using a Bioanalyzer (Agilent) ...
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bioRxiv - Genomics 2024Quote: Total RNA was isolated from 5 × 106 cells harvested from frozen patient vials using the RNeasy Plus mini-Kit (Qiagen). Libraries were prepared with the NEBNext® rRNA Depletion Kit and were sequenced using the Illumina NextSeq500 platform ...
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bioRxiv - Neuroscience 2024Quote: ... 100 µl of serum was centrifuged at 2000 x g for 5 min to remove debris and processed using the DNeasy Blood and Tissue Kit (Qiagen), with the ethanol precipitation step performed overnight at -20°C ...
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bioRxiv - Genomics 2024Quote: ... Each sample was mixed with 5 µL of one of 6 Illumina adapter sequences (specified in the Qiagen protocol appendix) and 2 µL of ultralow input ligase ...
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bioRxiv - Systems Biology 2024Quote: ... for 5 minutes and immediately lysed in the β-Mercaptoethanol-containing lysis solution from the RNeasy minikit (Qiagen Cat # 74104). RNA was extracted following the Qiagen kit instruction manual and eluted in 10mM Tris-HCl ...
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bioRxiv - Genomics 2024Quote: ... The pooled cells were centrifuged at 500 × g for 5 minutes at 4°C and resuspended in 100 μl of buffer EB (Qiagen).
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bioRxiv - Immunology 2024Quote: Parasite DNA quantification by qPCR was conducted on genomic DNA extracted from 5% of every brain homogenate using the DNEasy Blood & Tissue Kit (QIAGEN). As previously outlined (58) ...
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bioRxiv - Molecular Biology 2024Quote: ... The medium was thoroughly aspirated and replaced with 20 µl of 1x PBS containing 5 mg/ml of RNase A (Qiagen) and 12 mM of purified H6-mNeonGreen-NLS protein as a loading control ...
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bioRxiv - Immunology 2024Quote: Samples were double-sorted using a FACSAria or FACS Astrios: 600-1000 cells from each population were collected into 5 μl TCL buffer (Qiagen) containing 1% beta-mercaptoethanol (β-ME ...
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bioRxiv - Neuroscience 2024Quote: ... the anterior segment was pelleted by centrifugation for 5 minutes at 600g and then subjected to total RNA extraction using a RNeasy Mini Kit (QIAGEN). Following RNA extraction ...
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bioRxiv - Neuroscience 2024Quote: ... total RNA was extracted from the anterior half of the body from 10-12 5 dpf larvae per sample at using the Rneasy Mini Kit (Qiagen). cDNA was synthesized using the High-Capacity cDNA Reverse Transcription Kit (Applied Biosystems) ...
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bioRxiv - Physiology 2024Quote: Tissues were disrupted using stainless steel beads of 5 mm diameter in a Tissue Lyser LT (Qiagen, Venlo, The Netherlands). The resulting tissue homogenates were purified using the RNeasy Plus Mini Kit (Qiagen ...
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bioRxiv - Neuroscience 2024Quote: ... RNA was extracted from input and IP samples by incubation with 1 mL Trizol at room temperature for 5 minutes and then RNeasy Lipid Tissue Mini Kit (Qiagen) via the manufacturer’s protocol with on-column DNase treatment (Roche) ...
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bioRxiv - Cancer Biology 2024Quote: Genomic DNA was extracted from 5 IGH+ and 7 IGHUND lymphoma cases using either QIAamp DNA FFPE Tissue Kit (QIAGEN) (FFPE tissues ...
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bioRxiv - Zoology 2024Quote: ... Multiplex PCR was performed in 10 µl reaction volume containing 5 µl 2x QIAGEN Multiplex PCR Master Mix (QIAGEN, Germany), 2 µl 5x Q-Solution (QIAGEN) ...
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bioRxiv - Cancer Biology 2024Quote: ... and homogenised in NP40 lysis buffer (as described previously) using mechanical shearing with 5 mm stainless steel metal beads (Qiagen). Debris were cleared by centrifugation ...
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bioRxiv - Evolutionary Biology 2020Quote: ... 1 mL of stabilization mix (RNAprotect Bacteria Reagent - Qiagen diluted with PBS in a 2:1 volume ratio) was applied on plates ...
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bioRxiv - Developmental Biology 2021Quote: ... RNAs were isolated from 6-month H19ΔICR/H19ΔICR and H19+/H19+ littermates (2 per genotype) using RNeasy Plus Mini Kit (Qiagen). Samples with RNA Integrity numbers >9 were Ribosomal RNA depleted using RiboZero Gold Kit (Illumina) ...
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bioRxiv - Neuroscience 2020Quote: ... The 205bp amplicon from PCR 2 was gel extracted using the MinElute gel extraction kit (Qiagen Cat#: 28606), and sent to Genewiz for Amplicon-EZ targeted deep sequencing and Sanger sequencing ...
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bioRxiv - Cell Biology 2021Quote: ... The PCR #2 products were validated using gel electrophoresis and purified using the QIAquick Gel extraction kit (QIAGEN). The purified libraries were then quantified by Nanodrop and Qubit prior to sequencing on an Illumina MiSeq.
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bioRxiv - Systems Biology 2021Quote: ... Differences between samples and controls were calculated based on the 2−ΔΔCT method using RNU6 control primer (Qiagen) as normalizer ...
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bioRxiv - Plant Biology 2020Quote: ... Total RNA was extracted from 2-week-old Arabidopsis seedlings using an RNeasy Plant Mini Kit (Qiagen, Germany) according to the manufacturer’s instructions ...
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bioRxiv - Genomics 2021Quote: ... single cell directly into 2 µl lysis buffer (30 mM Tris-HCl [pH = 8.0], 10 mM NaCl, 0.2 µL Proteinase K [Qiagen, #19133] ...
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SARS-CoV-2 comprehensive receptor profiling: mechanistic insight to drive new therapeutic strategiesbioRxiv - Cell Biology 2021Quote: ... each pre-incubated at a 2:1 molar ratio with Penta His Alexa Fluor 647 Conjugate (Qiagen, UK). Hits (duplicate AF647 positive spots ...