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Citations for Qiagen :
1601 - 1650 of 10000+ citations for Rat Wide range C Peptide CP CLIA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genomics 2023Quote: ... The BD FACS Melody sorter device was used for sorting single cells into 96-well plates (FrameStar®, 4TI-0960/C) containing 2.5 μl RLT plus buffer (Qiagen). Plates were then spun down at 1,000g for 1 minute at 4°C ...
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bioRxiv - Genomics 2023Quote: ... to the eluted samples and digestion was carried out for 2 hours at 55°C followed by PCR purification (Qiagen). Sequencing libraries were prepared using the NEBNext Ultra II DNA Library Preparation Kit (New England Biolabs ...
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bioRxiv - Neuroscience 2023Quote: ... The remaining cells were pelleted at 1000 x g for 10 minutes at 4°C and lysed in 350 µL of Buffer RLT (Qiagen) supplemented with 3.5 µL of 2-β mercaptoethanol (#444203 ...
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Sweetwater: an underrated crude glycerol for sustainable lipid production in non-conventional yeastsbioRxiv - Systems Biology 2023Quote: ... tubes were vortexed for 15 s and submitted to cell disruption for 15-min at 4°C using a TissueLyser II (Qiagen) at 30 Hz ...
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bioRxiv - Cancer Biology 2023Quote: ... fresh 5 mM DTT) and incubated for 45 minutes at 65°C before addition of 40 μg Proteinase K (Qiagen) and further incubation for 1.5 hours at 37°C ...
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bioRxiv - Pathology 2023Quote: ... tissue and cells were lysed in RIPA lysis buffer supplemented with orthovanadate and a cocktail of protease inhibitors at 4°C (using a TissueLyser (Qiagen) to homogenize liver tissue ...
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bioRxiv - Evolutionary Biology 2023Quote: ... We collected three biological replicates of first molars from both species at three postnatal stages (P1, P15, and P21) and immediately preserved them at −80°C in lysis buffer (Buffer RLT; Qiagen) supplemented with 40 μM dithiothreitol ...
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bioRxiv - Genomics 2023Quote: ... tubes were incubated at 56°C for 1 hour followed by addition of 2ul of RNAse A (100mg/ml, QIAGEN) to 1ml of saliva sample and incubated at room temperature for 5 minutes ...
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bioRxiv - Microbiology 2023Quote: ... Lysates were centrifuged for 30 min at 38,000 g and 4°C and cleared supernatants loaded onto Ni-NTA columns with 0.5 mL bed volume (1018244 Qiagen, Germany). The columns were washed sequentially with 3 column volumes (CV ...
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bioRxiv - Molecular Biology 2023Quote: ... The resulting samples were supplemented with 300 mM NaCl and 10 mM imidazole and incubated overnight at 4 °C on a stirring wheel with 50 µl Ni-NTA agarose resin slurry (QIAGEN) pre-equilibrated with wash buffer I (50 mM Tris-HCl pH 7.8 ...
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bioRxiv - Microbiology 2023Quote: ... Cells were incubated at 37 °C with agitation for six hours after which time 1 mL cell pellets were treated with RNAprotect (Qiagen). Cell pellets were washed in 0.5 mL PBS pH 7.4 ...
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bioRxiv - Developmental Biology 2023Quote: ... Then samples and inputs were incubated for 4 hr at 37°C with 1.5 µl of 10 mg/ml RNase A (Qiagen, 1007885) and 15 µl 10% sodium dodecyl sulfate and 3.5 µl 20 mg/ml Proteinase K (Thermo Fisher ...
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bioRxiv - Microbiology 2024Quote: ... mixed with 1 mL phenol:chloroform:isoamyl alcohol (25:24:1 at pH 8) at 70°C for 12 min and bead beating for 2 min (Tissue Lyser II, Qiagen). The mixture was centrifuged at 4°C for 3 min at maximum speed and the aqueous phase was transferred to a new reaction tube ...
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bioRxiv - Microbiology 2024Quote: ... Bacteria are harvested by centrifugation (5 minutes, 4°C) and washed with 1 ml cold PBS containing RNA stabilization reagents (RNAprotect, Qiagen) before flash freezing and storage at -80°C ...
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bioRxiv - Biochemistry 2023Quote: ... Lysate was clarified by centrifugation at 5 °C at 18500 rpm for 30 min and loaded to gravity flow Ni-NTA agarose resin (QIAGEN) previously equilibrated with wash buffer WB3_1 (50 mM Tris-HCl pH 8 ...
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bioRxiv - Biochemistry 2023Quote: ... Lysates were clarified by centrifugation at 5 °C at 18 500 rpm for 30 min and loaded to gravity flow Ni-NTA agarose resin (QIAGEN) previously equilibrated with wash buffer WB2_1 (50 mM Tris-HCl pH 8 ...
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bioRxiv - Biochemistry 2024Quote: ... The remaining supernatant was centrifuged again at 5,000 x g for 20 minutes at 4°C and equilibrated for 1 hour in 3 mL of Ni-NTA resin (Qiagen) that was pre-equilibrated in the extraction buffer ...
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bioRxiv - Molecular Biology 2024Quote: ... Chromatin was treated with 1% SDS and 0.1 M NaHCO3 for 6 h at 65°C to reverse the cross-links and DNA was purified using columns (Qiagen) followed by ChIP-seq library preparation using ThruPLEX kit (Rubicon ...
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bioRxiv - Molecular Biology 2024Quote: ... The swabs were removed after heat incubation at 56 °C for 2 hours by transferring samples including swab material to QIAshredder tubes (Qiagen) and centrifugation at 12,000 rcf for 2 min.
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bioRxiv - Microbiology 2024Quote: ... Lysates were centrifuged for 30 min at 30000 x g in 4 °C and the resulting supernatants were gently mixed with Ni-NTA Agarose beads (Qiagen) pre-equilibrated with lysis buffer ...
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bioRxiv - Cancer Biology 2024Quote: ... Tumour samples were fresh-frozen in liquid nitrogen and stored at -80°C for protein isolation or preserved in RNAlater solution (Qiagen) for RNA isolation ...
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bioRxiv - Biochemistry 2024Quote: ... Lysed cells were sedimented by centrifugation at 20,000 RCF at 4 °C for 30 min and the lysate was subsequently loaded onto pre-equilibrated Ni-NTA agarose resin (Qiagen) at 4 °C ...
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bioRxiv - Biochemistry 2024Quote: ... The cell pellet was removed after centrifugation (16000 rpm, 4 °C, 1 h) and supernatant was loaded into a Ni-NTA agarose column (Qiagen). Protein was eluted in an elution buffer (50 mM Tris-HCl ...
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bioRxiv - Cell Biology 2024Quote: ... a second transfection of a 0.6 μg plasmid encoding either the C-terminal GFP11 tagged M2tail(368-466) or M2 receptor was performed using the Effectene Transfection Reagent (Qiagen). After washing each well with 2 mL PBS ...
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bioRxiv - Molecular Biology 2021Quote: ... Total genomic DNA extraction was performed using a commercial genomic DNA kit (DNeasy Blood & Tissue Kit, Qiagen). Photoproducts were quantified using a pre-optimized procedure (Thierry Douki ...
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bioRxiv - Immunology 2021Quote: Total RNA was isolated using the RNeasy Mini plus Kit or the RNeasy Micro plus Kit (Qiagen). After determining RNA concentrations by spectrophotometry ...
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bioRxiv - Microbiology 2021Quote: ... blank DNA extraction kits (N=14)) controls using the DNeasy PowerLyzer Powersoil kit (Qiagen, Germamtown, MD, USA), with minor modifications to the manufacturer’s protocols as previously described [109 ...
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bioRxiv - Physiology 2020Quote: ... DNA was isolated using Qiagen MagAttract PowerMicrobiome kit DNA/RNA kit (Qiagen, catalog no. 27500-4-EP) on the EpMotion 5075 (Eppendorf ...
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bioRxiv - Microbiology 2020Quote: ... RNA from Trizol samples was purified using a commercial kit (RNeasy® Mini Kit, Qiagen no. 74104) with additional on-column DNAse treatment (Qiagen no ...
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bioRxiv - Immunology 2020Quote: ... RNA was isolated using an RNeasy Mini Kit with the addition of RNase-free DNase kit (Qiagen) per manufacturer’s instructions ...
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bioRxiv - Microbiology 2020Quote: ... cellular RNA was extracted using the QIAshredder kit and the RNeasy Mini kit (79654 and 74106, Qiagen). The cDNA was generated using High Capacity cDNA reverse transcript kit (4368814 ...
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bioRxiv - Cancer Biology 2020Quote: Total RNA was isolated using an AllPrep DNA/RNA Mini kit or RNeasy Plus Micro kit (Qiagen). cDNA was prepared using the SuperScript III Reverse transcription Kit (Invitrogen) ...
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bioRxiv - Microbiology 2020Quote: ... The RNA extraction process was referred to the instructions of the kit (RNeasy Mini kit, QIAGEN, Germany). The extracted RNA was detected by ultra-fine ultraviolet spectrophotometer (NanoDrop ...
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bioRxiv - Plant Biology 2019Quote: ... The PCR product was purified by either QIAquick Gel Extraction Kit or QIAquick PCR Purification Kit (Qiagen), cloned into the donor vector pDONR207 using Gateway BP Clonase (Invitrogen) ...
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bioRxiv - Cell Biology 2019Quote: Total RNA was purified with RNeasy plus micro kit or RNeasy plus mini kit (Qiagen, Hilden, Dermany). Complementary DNA was synthesized by a SuperScript III First-Strand Synthesis System (Invitrogen) ...
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bioRxiv - Genetics 2021Quote: ... Genomic DNA was purified using the Xtreme DNA isolation kit (Isohelix) or DNeasy Blood & Tissue Kit (Qiagen). These kits were used to purify DNA from crude lysates or from tissue samples preserved in RNAlater solution (Sigma).
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bioRxiv - Microbiology 2021Quote: ... and viral DNA was extracted and purified using a commercial kit (DNeasy Blood and Tissue Kit, Qiagen) following manufacturer’s instructions ...
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bioRxiv - Immunology 2020Quote: ... Amplified scFv PCR fragments were purified with Gel Extraction kit (Qiaquick Gel Extraction Kit, Cat# 28706, QIAGEN), and subjected to SfiI (Fast Digest SfiI ...
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bioRxiv - Microbiology 2022Quote: gDNA was purified from Bacteroidales using a DNeasy UltraClean Microbial Kit or DNeasy Blood & Tissue Kit (Qiagen). Up to 2 μg in 200 μL TE buffer was sheared by ultrasonication to an average of 3–5 kb with a Covaris S220 focused ultrasonicator using red miniTUBEs ...
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bioRxiv - Microbiology 2023Quote: ... #342746 and #343061 using different DNA extraction kits: DNeasy Blood and Tissue kit (Qiagen GmbH, Hilden, Germany), and NucleoSpin Virus (Macherey-Nagel ...
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bioRxiv - Microbiology 2023Quote: ... We compared the extraction kits QIAamp UCP Pathogen Mini Kit (Cat No. 50214, Qiagen, Hilden, Germany; ‘Q’) versus ZymoBIOMICS DNA Microprep Kit (Cat No ...
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bioRxiv - Bioengineering 2023Quote: ... coli and purified for cell culture application through commercially available kits (Qiagen, Maxi Plasmid Kit, Cat# 12162).
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bioRxiv - Cancer Biology 2023Quote: ... Plasmid DNA from single colonies was extracted with the QIAprep Spin Miniprep Kit and Maxi Kits (Qiagen) and confirmed by Sanger sequencing (Genewiz ...
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bioRxiv - Bioengineering 2023Quote: ... before lysed using RLT buffer supplied in the RNeasy Mini Kit (Qiagen extraction kit, 74104, Qiagen, Germany). RNAs were collected by following the manufacturer’s protocol ...
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bioRxiv - Bioengineering 2023Quote: ... before lysed using RLT buffer supplied in the RNeasy Mini Kit (Qiagen extraction kit, 74104, Qiagen, Germany). RNAs were collected by following the manufacturer’s protocol ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... Total DNA was isolated from cells adherent cultures by Genomic Mini Kit (Qiagen Dneasy blood & tissue kit) according to the producer’s instructions ...
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bioRxiv - Microbiology 2024Quote: ... an EZ1 extraction was performed using a Qiagen kit with EZ1 DNA Tissue Kit (Qiagen, Courtaboeuf, France) in accordance with the manufacturer’s guidelines ...
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bioRxiv - Molecular Biology 2024Quote: ... Cells were harvested and total RNA isolated using an RNA isolation kit (RNeasy Mini kit, Qiagen, 74106). 8 µg of total RNA were treated with DNaseI and purified according to the manufacturer’s protocol (TURBO DNA-free kit ...
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bioRxiv - Microbiology 2024Quote: DNA was extracted using DNAeasy Power Soil Pro kit or DNAeasy PowerMax Soil kit (Qiagen, Kista, Sweden) for 47 mm and 142 mm filters ...
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bioRxiv - Neuroscience 2024Quote: ... Fig S1B) using the Qiagen RNeasy mini kit (RNeasy Mini Kit, Cat No. 74104, Qiagen, Hilden, Germany) ...